Crypthecodinium cohnii(dinoflagellate) and Schizochytrium sp.(thraustochytrid) are the main sources for docosahexaenoic acid(DHA). The present study aimed to evaluate the antioxidant activity of petroleum ether, ethyl...Crypthecodinium cohnii(dinoflagellate) and Schizochytrium sp.(thraustochytrid) are the main sources for docosahexaenoic acid(DHA). The present study aimed to evaluate the antioxidant activity of petroleum ether, ethyl acetate, n-butanol, and water fractions of alcohol aqueous extracts of these two microalgae and to provide a theoretical basis for comprehensive utilization. The antioxidant activity was determined by total antioxidant capacity(TAC) determination, 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging assay, ferrous ion-chelating ability(FICA) assay, and reducing power(RP) assay. The total phenolic content(TPC) and total flavonoid content(TFC) were also measured by the Folin-Ciocalteu and spectrophotometry methods, respectively. The results indicated that the extracts from these two microalgae possessed good antioxidant capacity. Analysis showed that most antioxidant performance indicators(TAC, DPPH, and RP) were positively correlated with the TPC of the extracts, suggesting that the phenolics might be the major components in C. cohnii and Schizochytrium sp., contributing to their antioxidative function. Therefore, the polar fractions of C. cohnii and Schizochytrium sp. could be further examined and considered for application in health products or cosmetics.展开更多
A total of 13 types of endophytic fungi,classified into six genera,were isolated from Hosta.ventricosa based on 18 S rDNA sequencing of ITS region and microscopic examination.The antioxidant activities of the crude ex...A total of 13 types of endophytic fungi,classified into six genera,were isolated from Hosta.ventricosa based on 18 S rDNA sequencing of ITS region and microscopic examination.The antioxidant activities of the crude extracts were investigated by T-AOC,DPPH and ABTS＋ methods.The results showed that the crude extracts from Fusarium oxysporum（HoV1） and Epicoccum（HoV4）,which presented higher phenol levels（1.90±0.03 mg/g,and 2.41±0.01 mg/g,respectively）,exhibited stronger comprehensive antioxidant activities,with higher T-AOC values（63.46±0.26 U/g,and 66.05±0.71 U/g,respectively）,lower EC_（50） values against ABTS~＋（30.25±0.05 μg/m L,and 24.66±0.65 μg/m L,respectively） and DPPH radicals（83.42±0.24 μg/m L,and 59.136 μg/m L,respectively）.Moreover,Fusarium oxysporum（Ho V8） showed excellent scavenging activity against DPPH radicals（EC_（50） = 16.02 μg/m L）,which was better than BHT.All of them could be useful in the developing of new natural antioxidant agents.展开更多
基金supported by the Shandong Academy of Agriculture Sciences(No.2015YQN32)the Ministry of Science and Technology of China(No.2014DFA32120)the National Natural Science Foundation of China(No.81471000)
文摘Crypthecodinium cohnii(dinoflagellate) and Schizochytrium sp.(thraustochytrid) are the main sources for docosahexaenoic acid(DHA). The present study aimed to evaluate the antioxidant activity of petroleum ether, ethyl acetate, n-butanol, and water fractions of alcohol aqueous extracts of these two microalgae and to provide a theoretical basis for comprehensive utilization. The antioxidant activity was determined by total antioxidant capacity(TAC) determination, 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging assay, ferrous ion-chelating ability(FICA) assay, and reducing power(RP) assay. The total phenolic content(TPC) and total flavonoid content(TFC) were also measured by the Folin-Ciocalteu and spectrophotometry methods, respectively. The results indicated that the extracts from these two microalgae possessed good antioxidant capacity. Analysis showed that most antioxidant performance indicators(TAC, DPPH, and RP) were positively correlated with the TPC of the extracts, suggesting that the phenolics might be the major components in C. cohnii and Schizochytrium sp., contributing to their antioxidative function. Therefore, the polar fractions of C. cohnii and Schizochytrium sp. could be further examined and considered for application in health products or cosmetics.
基金National Natural Science Foundation of China(Grant No.81703387)the Foundation for the demonstration of tea-fruit-medicine organic ecological safety belt in Ya’an
文摘A total of 13 types of endophytic fungi,classified into six genera,were isolated from Hosta.ventricosa based on 18 S rDNA sequencing of ITS region and microscopic examination.The antioxidant activities of the crude extracts were investigated by T-AOC,DPPH and ABTS＋ methods.The results showed that the crude extracts from Fusarium oxysporum（HoV1） and Epicoccum（HoV4）,which presented higher phenol levels（1.90±0.03 mg/g,and 2.41±0.01 mg/g,respectively）,exhibited stronger comprehensive antioxidant activities,with higher T-AOC values（63.46±0.26 U/g,and 66.05±0.71 U/g,respectively）,lower EC_（50） values against ABTS~＋（30.25±0.05 μg/m L,and 24.66±0.65 μg/m L,respectively） and DPPH radicals（83.42±0.24 μg/m L,and 59.136 μg/m L,respectively）.Moreover,Fusarium oxysporum（Ho V8） showed excellent scavenging activity against DPPH radicals（EC_（50） = 16.02 μg/m L）,which was better than BHT.All of them could be useful in the developing of new natural antioxidant agents.
