Scapharca broughtonii is a commercially important and over-exploited species.In order to investigate its genetic diversity and population structure,43 novel polymorphic microsatellites were isolated and characterized....Scapharca broughtonii is a commercially important and over-exploited species.In order to investigate its genetic diversity and population structure,43 novel polymorphic microsatellites were isolated and characterized.The number of alleles per locus ranged from 3 to 22 with an average of 6.93,and the observed and expected heterozygosities varied between 0.233 and 1.000,and 0.250 and 0.953,with an average of 0.614 and 0.707,respectively.Three highly informative multiplex PCRs were developed from nine of those microsatellites for S.broughtonii.We evaluated and validated these multiplex PCRs in 8 full-sib families.The average polymorphism information content(PIC) was 0.539.The frequency of null alleles was estimated as 3.13% of all the alleles segregation based on a within-family analysis of Mendelian segregation patterns.Parentage analysis of real offspring demonstrated that 100% of all offspring were unambiguously allocated to a pair of parents based on 3 multiplex sets.Those 43 microsatellite loci with high variability will be helpful for the analysis of population genetics and conservation of wild stock of S.broughtonii.The 3 sets of multiplex PCRs could be an important tool of pedigree reconstruction,population genetic analysis and brood stock management.展开更多
Based on multiple proxies from the Southern Hemisphere, an austral summer (December-January-February: DJF) Antarctic Oscillation Index (AAO) since 1500 A.D. was reconstructed with a focus on interannual to interdecada...Based on multiple proxies from the Southern Hemisphere, an austral summer (December-January-February: DJF) Antarctic Oscillation Index (AAO) since 1500 A.D. was reconstructed with a focus on interannual to interdecadal variability (<50 a). By applying a multivariate regression method, the observational AAO-proxy relations were calibrated and cross-validated for the period of 1957 89. The regressions were employed to compute the DJF-AAO index for 1500 1956. To verify the results, the authors checked the explained variance (r 2 ), the reduction of error (RE), and the standard error (SE). Cross-validation was performed by applying a leave-one-out validation method. Over the entire reconstruction period, the mean values of r 2 , RE, and SE are 59.9%, 0.47, and 0.67, respectively. These statistics indicate that the DJF-AAO reconstruction is relatively skillful and reliable for the last ～460 years. The reconstructed AAO variations on the interannual and interdecadal timescales compare favorably with those of several shorter sea level pressure (SLP)-based AAO indices. The leading periods of the DJF-AAO index over the last 500 years are ～2.4, ～2.6, ～6.3, ～24.1, and ～37.6 years, all of which are significant at the 95% level as estimated by power spectral analysis.展开更多
基金supported by National Marine Public Welfare Research Program(201205023)National Natural Science Foundation of China(41276138)Doctoral Program of Ministry of Education of China(2013 0132110009)
文摘Scapharca broughtonii is a commercially important and over-exploited species.In order to investigate its genetic diversity and population structure,43 novel polymorphic microsatellites were isolated and characterized.The number of alleles per locus ranged from 3 to 22 with an average of 6.93,and the observed and expected heterozygosities varied between 0.233 and 1.000,and 0.250 and 0.953,with an average of 0.614 and 0.707,respectively.Three highly informative multiplex PCRs were developed from nine of those microsatellites for S.broughtonii.We evaluated and validated these multiplex PCRs in 8 full-sib families.The average polymorphism information content(PIC) was 0.539.The frequency of null alleles was estimated as 3.13% of all the alleles segregation based on a within-family analysis of Mendelian segregation patterns.Parentage analysis of real offspring demonstrated that 100% of all offspring were unambiguously allocated to a pair of parents based on 3 multiplex sets.Those 43 microsatellite loci with high variability will be helpful for the analysis of population genetics and conservation of wild stock of S.broughtonii.The 3 sets of multiplex PCRs could be an important tool of pedigree reconstruction,population genetic analysis and brood stock management.
基金supported by the National Natural Science Foundation of China (Grant No. 40675035)the National High Technology Research and Development Program of China (Grant No. 2008AA121704)the National Key Technologies R&D Program of China (Grant No. 2009BAC51B05)
文摘Based on multiple proxies from the Southern Hemisphere, an austral summer (December-January-February: DJF) Antarctic Oscillation Index (AAO) since 1500 A.D. was reconstructed with a focus on interannual to interdecadal variability (<50 a). By applying a multivariate regression method, the observational AAO-proxy relations were calibrated and cross-validated for the period of 1957 89. The regressions were employed to compute the DJF-AAO index for 1500 1956. To verify the results, the authors checked the explained variance (r 2 ), the reduction of error (RE), and the standard error (SE). Cross-validation was performed by applying a leave-one-out validation method. Over the entire reconstruction period, the mean values of r 2 , RE, and SE are 59.9%, 0.47, and 0.67, respectively. These statistics indicate that the DJF-AAO reconstruction is relatively skillful and reliable for the last ～460 years. The reconstructed AAO variations on the interannual and interdecadal timescales compare favorably with those of several shorter sea level pressure (SLP)-based AAO indices. The leading periods of the DJF-AAO index over the last 500 years are ～2.4, ～2.6, ～6.3, ～24.1, and ～37.6 years, all of which are significant at the 95% level as estimated by power spectral analysis.
