项七针对椎动脉型颈椎病大兔模型血清一氧化氮、血浆内皮素、血栓素B_2含量的影响

目的:研究项七针对椎动脉型颈椎病大兔模型血清一氧化氮(NO)、血浆内皮素(ET)、血栓素B2(TXB2)含量的影响。方法:将27只正常新西兰白兔局部注射异体血液制成椎动脉型颈椎病大兔模型,随机等分为对照组、项七针组、颈夹脊穴组。对照组不予针刺治疗,项七针组、颈夹脊穴组分别针刺项七针和颈夹脊穴,并测定NO、ET、TXB2的含量。结果:项七针组、颈夹脊穴组针刺治疗后NO、ET、TXB2的改变优于对照组(P<0.05);项七针组优于颈夹脊穴组(P<0.05)。结论:针刺可引起血液内ET、NO、TXB2含量的变化,在治疗椎动脉型颈椎病方面项七针优于颈夹脊穴。

猪喘气病弱毒苗的研究 2.冻干大兔苗的研制和应用

自1985年以来,用猪肺炎霉形体兔化弱毒株(简称兔化弱毒株)接种大兔,用大兔肺研制猪喘气病弱毒冻干大兔苗15批,每批600—1200头份,在试验室接种猪62头,定期X光胸透观察60天,肺部呈阴性反应猪54头(87%),轻反应猪8头(12.9%),攻毒后保护49/62,保护率79%,12头健康对照猪全部发病(100%),15批苗安检、效检全部合格,在北京、河北等8省、市、自治区的30个猪场免疫接种不同月龄、不同品种猪10363头,观察2—3年,该苗安全,效果明显,免疫期8个月以上,在-20℃左右保存10个月。

胆宁片防治非酒精性脂肪肝的实验研究

目的研究胆宁片对大兔脂肪肝的防治作用。方法采用高胆固醇膳食致兔脂肪肝模型,研究胆宁片对大兔非酒精性脂肪肝的防治作用。结果模型组与胆宁片组的TC数值分别为(15.36±9.01)mg/dL、(2.67±5.35)mg/dL,TG分别为(1.11±0.42)mmol/L、(0.56±0.2)mmol/L,LDL-C分别为(13.16±7.39)mmol/L、(1.98+5.00)mmol/L,HDL-C分别为(0.49±0.23)mmol/L、(0.90±0.29)mmol/L,2组数据相比较,差异有显著性统计学意义(P<0.01),模型组新西兰兔的肝脏组织CYP7A1 mRNA表达较胆宁片组低,其中模型组与胆宁片组条带值分别为(0.56±0.14)(1.02±0.09),差异有统计学意义(P<0.001)。而模型组CYP2E1 mRNA表达较胆宁片组高,其中模型组与胆宁片组条带值分别为(1.49±0.31)(1.22±0.20),差异有统计学意义(P<0.05)。结论胆宁片对实验性大兔脂肪肝有一定的治疗作用。

Diagnose of Rabbit Pasteurellosis and Colibacillosis Mixed Infection and Drug Sensitive Test

In order to diagnose diseased rabbits, dead rabbits were dissected, and 4 strains, DX01, DX02, JX01 and JX02 were obtained from bacterial isolation and culture. According to conventional biochemical method, physiological-biochemical test showed that DX01 and DX02 could produce acids and gases in the presence of mannitol, lactose, glucose and maltose, and did not ferment sucrose, reactions of sodium citrate, urea, V-P, hydrogen sulfide and gelatin were negative, while reactions of indole, M-R and nitrate were positive, which were in accordance with the characteristics of colibacillus; and JX01 and JX02 both fermented mannitol, sucrose, glucose, maltose, and did not ferment lactose, reactions of indole, M-R, V-P, hydrogen sulfide and gelatin were negative, while the reaction of nitrate was positive, which were in accordance with the characteristics of Pasteurella. 16S rDNA detection and sequencing showed that JX01 and JX02 shared 100% homology with Pas- teurella. The animal inoculation test of isolated bacteria showed that the mice in the experiment groups all died within 2 d. Original cultures were isolated from dead mice. DX01 and DX02 were identified as colibacillus, JX01 and JX02 were identified as Pasteurella, and the death of diseased rabbits was caused by mixed infection of pasteureUosis and colibacillosis. Drug sensitive test showed that DX01 and DX02 were only sensitive to kanamycin, ofloxacin and sulfamethoxydiazine, and not sensitive to other drugs, and JX01 and JX02 were sensitive to ofloxacin, sulfamethoxydiazine, gentamicin, furazolidone, doxycycline, streptomycin, trimoxazole and spectinomycin, and were moderately sensitive to cefradine. Ofloxacin and sulfamethoxydiazine are preferable for the clinical treatment of this disease.

Preparation of High Titer Et Immune Serum by Low-Aged Animals

[Objective] The study aimed to establish an approach to high titer Et immune serum preparation by low-aged Japanese rabbits. [Method] Antigen of Et was prepared at first, Japanese rabbits were taken as immunized animal, and divided into two groups for experiments, one is two-month-old group （T group）, and another is six-month-old group （S group）. Japanese rabbits were continuous by immunized with low-dose by using auricular intravenous method, then immune sera were collected. Immune serum antibody titer was determined with micro-agglutination reaction method. E Resultl Agglutination reaction showed that the Et serum titer of S group is higher than that of T group in the first testing with the same dose. But in the second testing, the serum titer of the T group and and the S group was consistent, [Conclusien] The animals in the T group （Japanese rabbits） were fewer months old, and produced high titer antiserum was which consistent with the S group, which indicated that the method of preparing of high titer Et immune serum by low-aged Japanese rabbits was feasible.

纳雍:名源剌雍河

纳雍县位于毕节地区东南部,乌蒙山脉东南麓.东南与织金、六枝相邻,西南与水城为界,西北与毕节、赫章毗连,东北与大方相接.土地总面积2448.2平方公里.有汉、苗、彝等民族.

三株未知菌的鉴定和药物敏感性试验

本试验通过采集滥用抗生素的兔场中腹泻的、身体消瘦的大兔及不给小兔喂乳(且腹泻)的母兔脾脏和淋巴样品进行无菌检验,检出3株未知细菌。通过细菌分离、PCR扩增、药敏试验,得出3株菌分别是大肠杆菌和葡萄球菌,而且这3株菌对多种抗生素产生耐药性。由此可见,抗生素的规范使用必须得到重视,怀疑发生细菌性感染时,应根据药敏试验结果和国家相关规定合理用药。

Antibodies against the C-terminal peptide of rabbit oviductin inhibit mouse early embryo development to pass 2-cell stage

A full-length rabbit oviductin cDNA(1909bp) was cloned. It consists of a 5’-UTR of 52bp, an open reading frame (ORF) of 1374bp and a 3’-UTR of 483bp and has more than 80% homology with that of other mammal oviductins. N-terminal peptide (NTP) (384 residues) and C-terminal peptide (CTP) (73 residues) of deduced protein precursor has about 80% and 50% identity with that of other mammals respectively. Fusion proteins GST-NTP 368(1R-368N)and GST-CTP73 (369F-441A) were expressed and purified. NH2-terminal of CTP sequencing reveals that the purified protein is consistent with the deduced one. In order to study the function of NTP and CTP the mouse anti-NTP and rabbit anti-CTP antisera were prepared. Tissue-specific (skeleton muscle, oviduct, uterus, ovary, liver, heart and brain) analysis indicated that rabbit oviductin was only found in oviduct. The conditioned medium derived from the rabbit oviduct mucosa epithelial cells has a function of overcoming the early embryonic development block of Kunming mous e cultured in vitro. Anti-CTP antiserum could totally inhibit the early embryo development at 2-cell stage cultured in the conditioned culture medium, but anti-NTP antiserum couldn’t. There was a positive relationship between the ratio of early embryos at development block and the dosage of anti-CTP antiserum added in the conditioned culture medium. These results suggest that oviductin has a function not only on fertilization, but also on the release of early embryonic development block, and the later function domain of rabbit oviductin may be situate in its C-terminal.

Lung tissue flap repairs esophagus defection with an inner chitosan tube stent

AIM： TO repair the partial esophagus defect with a chitosan stent, a new esophageal prosthesis made of pulmonary tissue with vascular pedicie.METHODS： Fifteen Japanese big ear white rabbits were divided into experimental group （n = 10） and control group （n = 5）. Esophagus defect in rabbits of experimental group was repaired using lung tissue flap with a chitosan tube stent, gross and histological appearance was observed at week 2, 4 and 8 after operation, and barium sulphate X-ray screen was performed at week 10 after operation. Esophagus defect of rabbits in control group was repaired using lung tissue flap with no chitosan tube stent, gross and histological appearance was observed at week 2, 4 and 8 after operation, and barium sulphate X-ray screen was performed at week 10 after operation.RESULTS： In the experimental group, 6 rabbits survived for over two weeks, the lung tissue flap healed esophageal defection, and squamous metaplasia occurred on the surface of lung tissue flap. At week 10 after operation, barium sulphate examination found that barium was fluent through the esophagus with no stricture or back stream, the creeping was good. In the control group, 4 rabbits survived for two weeks, the lung tissue flap healed esophageal defection with fibrous tissue hyperplasia, barium sulphate examination found that barium was fluent through the esophagus with a slight stricture or back stream, and the creeping was not good at week 10 after operation.CONCLUSION： Esophagus defect can be repaired using lung tissue flap with an inner chitosan tube stent.

The Relationship Between Behavior with Some Productive Traits of Rabbits

This study aimed to obtain, by selection two lines of New Zealand white rabbits differing in total motor activity and to compare them for fertility, prolificacy, mortality and weight gains of young rabbits from birth until weaning. The emotional states of animals such as fear were analysed with the behavioural tests （open field, tonic immobility） and stress-induced hyperthermia （SIH）. These tests were the criterion for assigning animals to two experimental groups： group I, 10 males and 20 females with the highest total motor activity （A）; group II, 10 males and 20 females showing little or no motor activity （N）. Important differences were found in male and female fertility. During the first week, 80% of males mated in group I compared to only 20% in group II. Despite their normal breeding condition, passive males were timid and ponderous during mating, unlike active males which mated willingly and rapidly. Fertility was high in both groups （87.5% and 94.7% in group I and II）. Unlike females from group II, females from group I showed no tendency towards nest building. 40% of females from group I gave birth in different cage areas, while 90% of females from group II gave birth in nest boxes with down and straw bedding. No differences were found between the active and passive lines for body weight at birth or at 35 days of age.

Clinico-pathological Studies in Experimental Eimeria Infection in Domestic Rabbits （Oryctolagus Cuniculus）

Clinico-pathological study of experimental Eimeria species infection （coccidiosis） such as E. magna, E. intestinalis, E. irresidua, E. media and E. perforans in rabbits was conducted with dose inocula of 1 lac sporulated oocysts. These rabbits were divided into two groups each consisting of 12 rabbits. Two rabbits of infected untreated group died of severe disease on the 27^th ＆ 28^th day post infection. The intestine of dead animals showed elevated lesions in the middle half portion with congestion and destruction of villar epithelium.

EFFECT OF ELECTROACUPUNCTURE ON PLASMA ANGIOTENSIN-ALDOSTERONE AND ATRIAL NATRIURETIC POLYPEPTIDE IN RABBITS WITH ACUTE CEREBRAL INFARCTION

Objective: To observe the therapeutic effect of electroacupuncture (EA) on plasma angiotensin (Ang Ⅱ), aldosterone (ALD) and atrial natriuretic polypeptide (ANP) contents in experimental cerebral infarction rabbits for analyzing the underlying mechanism of acupuncture in ameliorating blood supply of the brain tissue. Methods: A total of 80 rabbits were randomized into control (n = 8), pseudo-operation (n = 24), model (n = 24) and EA (n = 24) groups. Cerebral infarction model was established by infusion of self-thrombus into the carotid artery. EA (1 mA, 2 Hz) was applied to 'Baihui'(百会 GV 20) and 'Shuigou'(水沟 GV 26) for 30 min, once every 12 hours. Plasma Ang Ⅱ, ALD and ANP contents were detected with radioimmunoassay method. In the later 3 groups, blood samples were taken at 6 h, 24 h and 48 h after cerebral ischemia. Results: Compared with control and pseudo-operation groups, Ang Ⅱ and ALD contents of model group at 6 h, 24 h and 48 h after cerebral ischemia increased significantly while plasma ANP of the 3 time-courses of model group decreased considerably (P<0.01). In comparison with model group, results showed that Ang-Ⅱ and ALD contents of EA group decreased significantly whereas ANP level of EA group increased strikingly (P<0.01). Conclusion: Electroacupuncture has the effects of raising plasma ANP level and lowering plasma Ang-Ⅱ and ALD in cerebral infarction rabbits.

复骨健步片对兔膝关节骨性关节炎组织形态学的实验研究

目的:观察复骨健步片对兔膝关节骨性关节炎组织形态学的影响,探讨其治疗膝关节骨性关节炎的机制。方法:选用48只新西兰大白兔,随机分为正常组(A组),模型组(B组),壮骨关节丸组(C组)和复骨健步片组(D组),每组12只,造模成功后,在第2、4、6周后每组随机提取4只制备标本,通过关节活动度、X线、光镜及电镜检查相关的组织病理变化。结果:关节活动度比较:D组与同期B、C组比较患膝活动度明显增大(P<0.05);X线表现:C、D组与B组比较,关节间隙轻微变窄,未见明显骨赘形成;光镜观察:D组软骨表面尚光滑,潮线完整,滑膜未见明显炎性细胞浸润,而同期B、C组无此修复性改变。按Colanbo和Markin的标准进行积分统计表明,D组在第4、6周评分比同期B、C组明显降低,统计学比较有显著性差异(P<0.05)。电镜观察:D组软骨细胞成簇增殖肥大,较B组明显改善。结论:复骨健步片具有抗炎、保护和修复关节软骨的作用。

Expression of Hsp70 and Caspase-3 in rabbits after se- vere traumatic brain injury

Objective： To investigate the expres- sion of Caspase-3 and Hsp70 in rabbits after severe trau- matic brain injury （TBI） and to explore the feasibility of its application in estimation of injury time in forensic medicine. Methods： Arabbit model of heavy TBI was developed by high velocity impact on the parietal bone with an iron stick. Totally 8 healthy adult New Zealand white rabbits were randomly divided into control group （n=2） and injury group （n=6）. Four hours after injury, tissue specimens from the parietal lobe, temporal lobe, occipital lobe, cerebellum and brainstem were harvested to detect the expression of Hsp70 and Caspase-3 by immunohistochemistry. Besides, the gray values of cells positive for HspT0 and Caspase-3 were analyzed with an image analyzer. Results： Immunohistochemistry staining demonstrated a low level of Caspase-3 and Hsp70 expression in normal control group. While in injury group, both the Caspase-3 and Hsp70 expression was significantly elevated （P〈0.05）. Positive cells gathered around the lesion focus. Occipital lobe and cerebellum had fewer positive cells while temporal and brainstem had the fewest. Conclusion： The expression of Caspase-3 and HspT0 at an early stage following severe TBI is characteristic and can be applied to estimate the time of injury.

Therapeutic effect of osteogenically induced adipose derived stem cells on vascular deprivation-induced osteonecrosis of the femoral head in rabbits

Objective： To explore the therapeutic ef- fect of osteogenically induced adipose-derived stem cells （ADSCs） on vascular deprivation-induced osteonecrosis of the femoral head （ONFH） in rabbit model. Methods： Vascular deprivation-induced ONFH was established by intramuscular injection of methylpre- dnisolone, and vascular occlusion of the capital femoral epiphysis by electrocoagulation in adult New Zealand white rabbits. Eight weeks afLer the establishment of vascular dep- rivation-induced ONFH, animals were randomly divided into three equal groups. In Group A （control）, no therapy was given. In Group B, core decompression was performed by drilling a hole （1.2 mm in diameter） from the outer cortex 2.5 cm distal to the proximal end of the greater trochanter. In Group C, 1 x 107 osteogenically induced ADSCs were resus- pended in 0.5 ml PBS, and then injected directly into the femoral head. Femoral head specimens were obtained at postoperative 8 weeks. The bone formation and three-di- mensional microstructure of the femoral head was evaluated by micro-computed tomography scans （u-CT）. Immunohistochemical analysis was performed to detect the expression ofosteocalcin. Angiogenesis and repair of the femoral head were observed histologically. Results： In trabecular bone at the proximal femur region, the trabecular volume was higher in Group C （130.70 mm3± 4.33 mm3） than that in Groups A （101.07 mm3±7.76 mm3） and B （107.89 mm3±8.6g mm3, P〈0.01）. Bone volume was significantly increased in Group C （40.09 mm3±6.35 mm3） than in Groups A （29.65 mm3±4.61 mm3） and B （31.80 mm3± 4.01 mm3, P〈0.01）. The trabecular number was higher in Groups C （1.58±0.25） than other two groups （1.15±0.18, 1.16± 0.21, P〈0.01）. Bone mineral density showed statistically significant difference between Groups C and A or B （375.38± 23.06） mg HA/ccm, vs （313.73 ± 19.30） mg HA/ccm and （316.09± 16.45） mg HA/ccm, P〈0.01）. Histological examination indicated that there was more new bone formation in Group C than in other groups. Conclusion： Treatment with autologous osteogenically induced ADSCs transplantation results in an enhanced osteogenesis and microstructure of the vascular deprivation-induced osteonecrosis in rabbits.