[Objective] The aim was to provide a reference for studying the resistant ability of subtropical forest to freeze disaster and detecting the recovery and evolution of Huodendron biaristatum species after freeze disast...[Objective] The aim was to provide a reference for studying the resistant ability of subtropical forest to freeze disaster and detecting the recovery and evolution of Huodendron biaristatum species after freeze disaster.[Method] Effects of the damage caused by the southern ice storm in early 2008 on Huodendron biaristatum,dominant species of evergreen broad-leaved forest on the Damingshan,were studied in the aspect of population ecology.[Result] Among the 1 090 Huodendron biaristatum individuals （DBH ≥ 1 cm）,697 individuals were victims,accounting for 63.9%.The largest part of the victims was crown breakdown,which took up 37.6%.13.0% of the victims were bent,and 10.2% of the victims were trunk broken.The least part of the victims were uprooted,accounting for only 3.1%.Different types and extent of forestry damage had remarkable differences in diameter class,tree height,crown and taper.Rates of damage and crown breakdown increased with the increase of DBH,while bent rate decreased.Rates of damage,bent and trunk broken declined slowly with the increase of tree height,while crown breakdown rate followed the normal distribution.As the crown level increased,rates of damage and crown breakdown increased,while the rate of trunk broken decreased.Individuals with high taper were more susceptible to crown damage,bending,breaking and uprooted.[Conclusion] The resistant ability of Huodendron biaristatum species to freeze disaster is affected by many factors like DBH,tree height,crown,taper,etc..Huodendron biaristatum species in Damingshan affected by those factors show various kinds of damaged types dominant by mechanical damage,but there is few individual to die.展开更多
As a widely distributed geological and engineering material,the soil-rock mixture always undergoes frequentative and short-term freeze-thaw cycles in some regions.Its internal structure is destroyed seriously,but the ...As a widely distributed geological and engineering material,the soil-rock mixture always undergoes frequentative and short-term freeze-thaw cycles in some regions.Its internal structure is destroyed seriously,but the damage mechanism is not clear.Based on the damage factor,the damage research of properties of soil-rock mixture after different times of freeze-thaw cycles is investigated.Firstly,the size-distributed subgrade gravelly soil samples are prepared and undergo different times of freeze-thaw cycles periodically(0,3,6,10),and indoor large-scale triaxial tests are completed.Secondly,the degradation degree of elastic modulus is considered as a damage factor,and applied to macro damage analysis of soil-rock mixture.Finally,the mesoscopic simulation of the experiments is achieved by PFC3D,and the influence on strength between soil-rock particles caused by freeze-thaw cycles is analyzed.The results show that freeze-thaw cycles cause internal damage of samples by weakening the strength between mesoscopic soil-rock particles,and ultimately affect the macro properties.After freeze-thaw cycles,on the macro-scale,elastic modulus and shear strength of soil-rock mixture both decrease,and the decreasing degree is related to the times of cycles with the mathmatical quadratic form;on the meso-scale,freeze-thaw cycles mainly cause the degradation of the strength between soil-rock particles whose properties are different significantly.展开更多
Objective： The aim of our study was to observe the survival and morphological changes of thawed ovarian tis- sues after heterotopic transplantation. Methods： Twenty SPF-SD female rats （5-6 weeks old） were equally ...Objective： The aim of our study was to observe the survival and morphological changes of thawed ovarian tis- sues after heterotopic transplantation. Methods： Twenty SPF-SD female rats （5-6 weeks old） were equally randomized into the control group and experimental group. In control group, the freshly isolated ovaries were fixed in formalin. In experimental group, the freshly isolated ovaries were vitrified immediately and cut into thin slices. After stored in liquid nitrogen for 21 days, the tissues of experimental group were rapidly thawed and transplanted into back muscles of rats for 2 or 4 weeks, respectively. After that, all rats in experimental group were sacrificed and the ovarian tissues were collected and fixed in 4% formaldehyde solution. Then the ovarian tissues were stained with HE and observed under the light confocal microscope. Re- suits： With the naked eyes, there was no specific alteration except the size reduction with color changing. Under microscopy, we found normal cortex and medulla in the ovary, and the primordial follicles and follicles in various stages were observed in the cortex. The normal oocytes in ovarian tissues of experimental group were significant decreased than in the control group. Conclusion： The ovarian tissues survive well in experimental group and there is no significant difference in the proportion of follicles between different times （2 and 4 weeks） after grafting. Our results suggest that thawed ovarian tissues could survive after heterotopic transplantation into back muscles of rat models and maintain their morphology and function.展开更多
Objective: The aim of the study was to observe and compare the effects of cryopreservation and thawing meth- ods on rat ovarian tissues. Methods: Twenty 5-6 weeks old SPF-SD female rats were randomly divided into two ...Objective: The aim of the study was to observe and compare the effects of cryopreservation and thawing meth- ods on rat ovarian tissues. Methods: Twenty 5-6 weeks old SPF-SD female rats were randomly divided into two groups, with ten rats in each group. Freshly isolated ovaries saved as a control (group 1: fresh ovaries) in formalin-fixed or vitrified immediately after dissection (group 2: vitrified ovaries). Ovaries in vitrified group were processed into thin slices then cryo- preserved, stored in liquid nitrogen for 21 days, rapidly thawed and grossly examined. All of the collected ovaries underwent hematoxylin and eosin-stained paraffin serial sections and observed the microscopic evaluation in vitrified ovaries. Results: Grossly the vitrified ovaries turned pale color and the size was same as before freeze. The vitrified ovarian tissue had normal anatomical structures of cortex and medulla under the microscope and had no difference with the fresh control ovarian tis- sue. The number and distribution of the follicles were similar with the fresh ovarian tissue, but had smaller size and the gap between oocyte and the surrounding granulosa cells was increased. Few ooctyes were in irregular appearance however the morphology of follicular cells did not give a different appearance as compared to the fresh control ovarian tissue. Conclusion: Cryopreservation of ovarian tissues by vitrification method has some detrimental effect on the morphology of follicles but does not induce negative impact on the number, density and survival of the primordial ovarian follicles. However the whole follicle anatomical structures also had no significant changes.展开更多
基金Support by National Natural Science Foundation of China(30860059)~~
文摘[Objective] The aim was to provide a reference for studying the resistant ability of subtropical forest to freeze disaster and detecting the recovery and evolution of Huodendron biaristatum species after freeze disaster.[Method] Effects of the damage caused by the southern ice storm in early 2008 on Huodendron biaristatum,dominant species of evergreen broad-leaved forest on the Damingshan,were studied in the aspect of population ecology.[Result] Among the 1 090 Huodendron biaristatum individuals （DBH ≥ 1 cm）,697 individuals were victims,accounting for 63.9%.The largest part of the victims was crown breakdown,which took up 37.6%.13.0% of the victims were bent,and 10.2% of the victims were trunk broken.The least part of the victims were uprooted,accounting for only 3.1%.Different types and extent of forestry damage had remarkable differences in diameter class,tree height,crown and taper.Rates of damage and crown breakdown increased with the increase of DBH,while bent rate decreased.Rates of damage,bent and trunk broken declined slowly with the increase of tree height,while crown breakdown rate followed the normal distribution.As the crown level increased,rates of damage and crown breakdown increased,while the rate of trunk broken decreased.Individuals with high taper were more susceptible to crown damage,bending,breaking and uprooted.[Conclusion] The resistant ability of Huodendron biaristatum species to freeze disaster is affected by many factors like DBH,tree height,crown,taper,etc..Huodendron biaristatum species in Damingshan affected by those factors show various kinds of damaged types dominant by mechanical damage,but there is few individual to die.
基金Project(50908234)supported by the National Natural Science Foundation of ChinaProject(2017G002-K)supported by the Key Subject of Science and Technology Research and Development Plan of China Railway General Corporation
文摘As a widely distributed geological and engineering material,the soil-rock mixture always undergoes frequentative and short-term freeze-thaw cycles in some regions.Its internal structure is destroyed seriously,but the damage mechanism is not clear.Based on the damage factor,the damage research of properties of soil-rock mixture after different times of freeze-thaw cycles is investigated.Firstly,the size-distributed subgrade gravelly soil samples are prepared and undergo different times of freeze-thaw cycles periodically(0,3,6,10),and indoor large-scale triaxial tests are completed.Secondly,the degradation degree of elastic modulus is considered as a damage factor,and applied to macro damage analysis of soil-rock mixture.Finally,the mesoscopic simulation of the experiments is achieved by PFC3D,and the influence on strength between soil-rock particles caused by freeze-thaw cycles is analyzed.The results show that freeze-thaw cycles cause internal damage of samples by weakening the strength between mesoscopic soil-rock particles,and ultimately affect the macro properties.After freeze-thaw cycles,on the macro-scale,elastic modulus and shear strength of soil-rock mixture both decrease,and the decreasing degree is related to the times of cycles with the mathmatical quadratic form;on the meso-scale,freeze-thaw cycles mainly cause the degradation of the strength between soil-rock particles whose properties are different significantly.
文摘Objective： The aim of our study was to observe the survival and morphological changes of thawed ovarian tis- sues after heterotopic transplantation. Methods： Twenty SPF-SD female rats （5-6 weeks old） were equally randomized into the control group and experimental group. In control group, the freshly isolated ovaries were fixed in formalin. In experimental group, the freshly isolated ovaries were vitrified immediately and cut into thin slices. After stored in liquid nitrogen for 21 days, the tissues of experimental group were rapidly thawed and transplanted into back muscles of rats for 2 or 4 weeks, respectively. After that, all rats in experimental group were sacrificed and the ovarian tissues were collected and fixed in 4% formaldehyde solution. Then the ovarian tissues were stained with HE and observed under the light confocal microscope. Re- suits： With the naked eyes, there was no specific alteration except the size reduction with color changing. Under microscopy, we found normal cortex and medulla in the ovary, and the primordial follicles and follicles in various stages were observed in the cortex. The normal oocytes in ovarian tissues of experimental group were significant decreased than in the control group. Conclusion： The ovarian tissues survive well in experimental group and there is no significant difference in the proportion of follicles between different times （2 and 4 weeks） after grafting. Our results suggest that thawed ovarian tissues could survive after heterotopic transplantation into back muscles of rat models and maintain their morphology and function.
文摘Objective: The aim of the study was to observe and compare the effects of cryopreservation and thawing meth- ods on rat ovarian tissues. Methods: Twenty 5-6 weeks old SPF-SD female rats were randomly divided into two groups, with ten rats in each group. Freshly isolated ovaries saved as a control (group 1: fresh ovaries) in formalin-fixed or vitrified immediately after dissection (group 2: vitrified ovaries). Ovaries in vitrified group were processed into thin slices then cryo- preserved, stored in liquid nitrogen for 21 days, rapidly thawed and grossly examined. All of the collected ovaries underwent hematoxylin and eosin-stained paraffin serial sections and observed the microscopic evaluation in vitrified ovaries. Results: Grossly the vitrified ovaries turned pale color and the size was same as before freeze. The vitrified ovarian tissue had normal anatomical structures of cortex and medulla under the microscope and had no difference with the fresh control ovarian tis- sue. The number and distribution of the follicles were similar with the fresh ovarian tissue, but had smaller size and the gap between oocyte and the surrounding granulosa cells was increased. Few ooctyes were in irregular appearance however the morphology of follicular cells did not give a different appearance as compared to the fresh control ovarian tissue. Conclusion: Cryopreservation of ovarian tissues by vitrification method has some detrimental effect on the morphology of follicles but does not induce negative impact on the number, density and survival of the primordial ovarian follicles. However the whole follicle anatomical structures also had no significant changes.
