栖息地丧失和退化严重威胁全球两栖类动物的生存与繁衍,栖息地保护在两栖动物保育中具有高的优先性。大凉螈(Liangshantriton taliangensis)是国内外广泛关注的横断山区特有珍稀有尾类物种。结合大凉螈33个分布位点和9个环境变量数据,...栖息地丧失和退化严重威胁全球两栖类动物的生存与繁衍,栖息地保护在两栖动物保育中具有高的优先性。大凉螈(Liangshantriton taliangensis)是国内外广泛关注的横断山区特有珍稀有尾类物种。结合大凉螈33个分布位点和9个环境变量数据,本文运用最大熵(MaxEnt)模型预测了大凉螈当前潜在适宜分布区,再根据现有自然保护地(5个国家级和9个省级)分布进行了保护空缺分析,同时,借助比例体重指数法(SMI)衡量了7个种群共218个个体的身体状况,比较了保护区内外种群间的差异,利用归一化植被指数(NDVI)识别了保护区内植被变化,据此对四川栗子坪国家级自然保护区的保护成效进行了评估。结果表明,(1)影响大凉螈分布的最主要环境变量为NDVI(贡献值34.33%)、最干月份降水量(贡献值26.81%)和海拔(贡献值20.92%);(2)大凉螈适宜生境主要集中在小相岭和凉山山系,占研究区域总面积的15.38%,现有自然保护地覆盖了大凉螈23.62%的适宜生境,仍有3760.91km^(2)的保护空缺;(3)栗子坪保护区内雄性个体肥满度指数显著大于保护区外个体(t-test,P=0.006),雌性个体肥满度指数无显著差异(t-test,P=0.214),保护区内近6年NDVI值显著高于保护区建立前(Mann-Whitney U test,P<0.0001),均提示总体保护成效优。结合研究结果,提出了确立大凉螈为重要保护目标物种、扩大现有保护区、建立保护小区或拯救中心、建设生态廊道等保护地建设的物种恢复措施,以提升对大凉螈的保护能力。展开更多
Alkaline phosphatase(AKP)was isolated and purified from the skin of Tylototriton taliangensis Liu and its kinetic property was examined.The partially purified alkaline phosphatase was purified by salting\|out,CM\|cell...Alkaline phosphatase(AKP)was isolated and purified from the skin of Tylototriton taliangensis Liu and its kinetic property was examined.The partially purified alkaline phosphatase was purified by salting\|out,CM\|cellulose ion\|exchange column and gel filtration with Sephadex G\|150.The purified enzyme from skin moved as a single electrophoretic band in PAGE.The specific activity was 90.26 units/mg protein.Its subunit weight was 42.0 kD as determined with SDS\|PAGE.The optimum pH value for the enzyme was pH9.0.The optimum temperature was about 34℃.The Michealis\|Menton constant( K \-m)was 0.83 mmol/L on the disodium phenyl phosphate.Activity differences of the enzymes were determined when metal ions effected on the AKP.The results showed that K + was found to have no inhibition of AKP activity.Mg 2+ ,Ca 2+ ,Cu 2+ could activate the AKP and the higher the metal ions concentration were,the more the activity of AKP increased.When 3.0 mmol/L Cu 2+ was used,the activity of AKP could rise to 187%.Ni 2+ ,Zn 2+ and Ag + could inhibit the AKP and the higher the metal ions concentration were,the more the activity of AKP decreased.When 3.0 mmol/L Ag + was used,the activity of AKP retained 23% only.展开更多
The full coding region of brain derived neurotrophic factor (BDNF) gene from Tylototriton taliangensis genome was amplified by PCR with the primers designed according to the sequence of human BDNF gene. Sequence analy...The full coding region of brain derived neurotrophic factor (BDNF) gene from Tylototriton taliangensis genome was amplified by PCR with the primers designed according to the sequence of human BDNF gene. Sequence analysis shows that the coding region has 744 bp in length and is highly homologous with that of mammals and aves. The homology of deduced BDNF protein sequence is about 90% with that of human. It is the first time to amplify and analyze BDNF gene from the newt genome, which will provide useful information for protecting and studying the severely endangered species. Fig1,Tab1,Ref展开更多
文摘栖息地丧失和退化严重威胁全球两栖类动物的生存与繁衍,栖息地保护在两栖动物保育中具有高的优先性。大凉螈(Liangshantriton taliangensis)是国内外广泛关注的横断山区特有珍稀有尾类物种。结合大凉螈33个分布位点和9个环境变量数据,本文运用最大熵(MaxEnt)模型预测了大凉螈当前潜在适宜分布区,再根据现有自然保护地(5个国家级和9个省级)分布进行了保护空缺分析,同时,借助比例体重指数法(SMI)衡量了7个种群共218个个体的身体状况,比较了保护区内外种群间的差异,利用归一化植被指数(NDVI)识别了保护区内植被变化,据此对四川栗子坪国家级自然保护区的保护成效进行了评估。结果表明,(1)影响大凉螈分布的最主要环境变量为NDVI(贡献值34.33%)、最干月份降水量(贡献值26.81%)和海拔(贡献值20.92%);(2)大凉螈适宜生境主要集中在小相岭和凉山山系,占研究区域总面积的15.38%,现有自然保护地覆盖了大凉螈23.62%的适宜生境,仍有3760.91km^(2)的保护空缺;(3)栗子坪保护区内雄性个体肥满度指数显著大于保护区外个体(t-test,P=0.006),雌性个体肥满度指数无显著差异(t-test,P=0.214),保护区内近6年NDVI值显著高于保护区建立前(Mann-Whitney U test,P<0.0001),均提示总体保护成效优。结合研究结果,提出了确立大凉螈为重要保护目标物种、扩大现有保护区、建立保护小区或拯救中心、建设生态廊道等保护地建设的物种恢复措施,以提升对大凉螈的保护能力。
文摘Alkaline phosphatase(AKP)was isolated and purified from the skin of Tylototriton taliangensis Liu and its kinetic property was examined.The partially purified alkaline phosphatase was purified by salting\|out,CM\|cellulose ion\|exchange column and gel filtration with Sephadex G\|150.The purified enzyme from skin moved as a single electrophoretic band in PAGE.The specific activity was 90.26 units/mg protein.Its subunit weight was 42.0 kD as determined with SDS\|PAGE.The optimum pH value for the enzyme was pH9.0.The optimum temperature was about 34℃.The Michealis\|Menton constant( K \-m)was 0.83 mmol/L on the disodium phenyl phosphate.Activity differences of the enzymes were determined when metal ions effected on the AKP.The results showed that K + was found to have no inhibition of AKP activity.Mg 2+ ,Ca 2+ ,Cu 2+ could activate the AKP and the higher the metal ions concentration were,the more the activity of AKP increased.When 3.0 mmol/L Cu 2+ was used,the activity of AKP could rise to 187%.Ni 2+ ,Zn 2+ and Ag + could inhibit the AKP and the higher the metal ions concentration were,the more the activity of AKP decreased.When 3.0 mmol/L Ag + was used,the activity of AKP retained 23% only.
文摘The full coding region of brain derived neurotrophic factor (BDNF) gene from Tylototriton taliangensis genome was amplified by PCR with the primers designed according to the sequence of human BDNF gene. Sequence analysis shows that the coding region has 744 bp in length and is highly homologous with that of mammals and aves. The homology of deduced BDNF protein sequence is about 90% with that of human. It is the first time to amplify and analyze BDNF gene from the newt genome, which will provide useful information for protecting and studying the severely endangered species. Fig1,Tab1,Ref
