[Objective] This study was to find out the artificial diet formula for Erigoni- dium graminicolum （Sundevall） to realize its indoor artificial breeding. [Method] Using orthogonal decomposition method, the diet most ...[Objective] This study was to find out the artificial diet formula for Erigoni- dium graminicolum （Sundevall） to realize its indoor artificial breeding. [Method] Using orthogonal decomposition method, the diet most suitable for the normal and quick growth of Erigonidium graminicolum （sundevall）, was developed by adding different species and different proportions of fresh insects to the artificial diet based on the formula of total nutrient solution proposed by Zhao Jing-zhao. [Result] The most suit- able formula for the development and growth of Erigonidium graminicolum （Sundevall） is 10 ml of egg white, 2 g of honey, 2 g of sucrose, 10 ml of water, 5 g of Ci- cadella viridis, moderate amount of cornmeal, compound vitamin B1 and vitamin C1. [Conclusion] The development of new type artificial diet can provide timely and nec- essary material premise for its large-scale indoor propagation.展开更多
In order to evaluate the anti-influenza virus activity of the effective monomer from Folium Isatidis (FI) in vivo,we established mice model with viral pneumonia and divided them into 3 different dose groups,then obser...In order to evaluate the anti-influenza virus activity of the effective monomer from Folium Isatidis (FI) in vivo,we established mice model with viral pneumonia and divided them into 3 different dose groups,then observed their lung indexes,pulmonary pathological changes,pulmonary virus hemagglitination titers,living time and death rates.The results showed that the monomer could reduce the pulmonary index from 2.64 to 1.93,1.63 and 1.40 (P<0.01) and decrease the hemagglitination titer from 1.15 to 0.84,0.70 and 0.59 (P<0.01).In addition,different groups of FI could significantly lessen the mortality rate from 100% to 30%,25% and 15%,and prolong the living time from 5.1d to 6.5d,8.4d and 8.9d respectively(P<0.01).The high dose (75 mg/kg/d) has the similar effect with 100 mg/kg/d dose of virazole(P>0.05),and more effective than 200 mg/kg/d dose of antiviral liquor (P<0.05).展开更多
Traditional Chinese medicine （TCM） has been widely used in China for thousands of years, and has received more and more recognition worldwide. Indigoplant （Folium Polygoni Tinctorii）, a TCM, has been used in vario...Traditional Chinese medicine （TCM） has been widely used in China for thousands of years, and has received more and more recognition worldwide. Indigoplant （Folium Polygoni Tinctorii）, a TCM, has been used in various diseases. In our study, we established a quality standard of Indigoplant according to the European Pharmacopoeia, and this standard has passed the early audit stages of the European Pharmacopoeia Commission. The lndigoplant samples were identified with high performance thin layer chromatography （HPTLC）, qualified with RP-HPLC quantitation, and analyzed with a series of quality tests. An accurate, reliable and robust HPLC method with gradient elution for quantitation was developed and validated with a one-variable-at-a-time （OVAT） robustness approach. Several tests, including the loss on drying, total ash and ash insoluble in hydrochloric acid of Indigoplant, were performed for quality analysis. Furthermore, six batches of the Indigoplant samples were appraised with this quality standard. In conclusion, the established quality standard was more internationally normative and applicable for the quality control of Indigoplant in practical application.展开更多
文摘[Objective] This study was to find out the artificial diet formula for Erigoni- dium graminicolum （Sundevall） to realize its indoor artificial breeding. [Method] Using orthogonal decomposition method, the diet most suitable for the normal and quick growth of Erigonidium graminicolum （sundevall）, was developed by adding different species and different proportions of fresh insects to the artificial diet based on the formula of total nutrient solution proposed by Zhao Jing-zhao. [Result] The most suit- able formula for the development and growth of Erigonidium graminicolum （Sundevall） is 10 ml of egg white, 2 g of honey, 2 g of sucrose, 10 ml of water, 5 g of Ci- cadella viridis, moderate amount of cornmeal, compound vitamin B1 and vitamin C1. [Conclusion] The development of new type artificial diet can provide timely and nec- essary material premise for its large-scale indoor propagation.
基金Natural Science Foundation of South Central University for Nationalities (YZQ05011)
文摘In order to evaluate the anti-influenza virus activity of the effective monomer from Folium Isatidis (FI) in vivo,we established mice model with viral pneumonia and divided them into 3 different dose groups,then observed their lung indexes,pulmonary pathological changes,pulmonary virus hemagglitination titers,living time and death rates.The results showed that the monomer could reduce the pulmonary index from 2.64 to 1.93,1.63 and 1.40 (P<0.01) and decrease the hemagglitination titer from 1.15 to 0.84,0.70 and 0.59 (P<0.01).In addition,different groups of FI could significantly lessen the mortality rate from 100% to 30%,25% and 15%,and prolong the living time from 5.1d to 6.5d,8.4d and 8.9d respectively(P<0.01).The high dose (75 mg/kg/d) has the similar effect with 100 mg/kg/d dose of virazole(P>0.05),and more effective than 200 mg/kg/d dose of antiviral liquor (P<0.05).
基金Program for Liaoning Innovative Research Team in University,Key Technologies in Quality Control of Traditional Chinese Medicines(Grant No.LT2012018)
文摘Traditional Chinese medicine （TCM） has been widely used in China for thousands of years, and has received more and more recognition worldwide. Indigoplant （Folium Polygoni Tinctorii）, a TCM, has been used in various diseases. In our study, we established a quality standard of Indigoplant according to the European Pharmacopoeia, and this standard has passed the early audit stages of the European Pharmacopoeia Commission. The lndigoplant samples were identified with high performance thin layer chromatography （HPTLC）, qualified with RP-HPLC quantitation, and analyzed with a series of quality tests. An accurate, reliable and robust HPLC method with gradient elution for quantitation was developed and validated with a one-variable-at-a-time （OVAT） robustness approach. Several tests, including the loss on drying, total ash and ash insoluble in hydrochloric acid of Indigoplant, were performed for quality analysis. Furthermore, six batches of the Indigoplant samples were appraised with this quality standard. In conclusion, the established quality standard was more internationally normative and applicable for the quality control of Indigoplant in practical application.
