大孔吸附树脂提取纯化通光散中总甾苷的工艺研究

目的:优选通光散乙醇提取液经树脂纯化的最佳工艺条件。方法:用正交试验法进行优选,以二氢肉珊瑚苷元为指标测定总甾苷含量。结果:吸附工艺的最佳条件为A_3B_2C_2D_3,即药液浓度为4mg·mL^(-1),温度为45℃,流速为2.0BV·h^(-1),径高比1:10,解吸附工艺的最佳条件为A_2B_3C_2,即洗脱剂用量8BV、洗脱剂温度65℃、洗脱流速2BV·h^(-1)。结论:通光散的乙醇提取物经大孔树脂吸附纯化后总甾苷含量明显增加,本研究为通光散中总甾苷的制备工艺提供依据。

大孔吸附树脂分离纯化胡黄连中胡黄连苷Ⅱ的工艺研究

胡黄连为玄参科植物胡黄连（Picrorhiza scrophu-lariiflora Pennell）的干燥根茎,是一味传统中药,具有清热凉血、燥湿消疳的作用,用于小儿惊痫、疳积、泻痢、骨蒸劳热、自汗、盗汗、吐血、目疾、痔瘘、疮肿等。近年来国内外研究发现,

大孔吸附树脂法纯化黄花草木樨总皂苷工艺的研究

目的优化大孔吸附树脂法纯化黄花草木樨叶中总皂苷的工艺。方法:以黄花草木樨中总皂苷的含量为指标,考察了5种大孔吸附树脂对黄花草木樨中总皂苷的吸附及解吸效果,筛选出最适皂苷纯化的树脂,并对纯化工艺进行了优化。结果:AB-8大孔吸附树脂适合纯化黄花草木樨中的总皂苷,最佳洗脱条件为:上样浓度为1.0mg/ml,上柱吸附流速为2.0 ml·min-1,以70%乙醇洗脱,洗脱流速2.0ml·min-1,洗脱体积为5 BV。结论所选工艺方法简便,稳定,纯化效果好。

鸡血藤原花青素的纯化及活性评价

采用大孔吸附树脂对鸡血藤原花青素进行纯化,并对原花青素纯度、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除活性及α-葡萄糖苷酶抑制活性进行评价。比较3种大孔吸附树脂对原花青素静态吸附及解吸附能力,从D101、X-5及AB-8树脂筛选出X-5型树脂用于纯化。对X-5型树脂的动态吸附及解吸附条件进行优化,获得最适条件为:上样质量浓度6.00 mg/mL,上样流速2 BV/h,上样量10 BV,洗脱流速1 BV/h,洗脱剂用量2 BV。利用不同体积分数乙醇洗脱可得到不同纯度的原花青素,其中70%乙醇纯化物原花青素纯度最高,具有最强的DPPH自由基清除活性及α-葡萄糖苷酶抑制活性。相关性分析表明原花青素可能是鸡血藤抗氧化及抑制α-葡萄糖苷酶的主要活性成分。

三七总皂苷分离纯化工艺研究

目的研究D-101型大孔吸附树脂分离纯化三七总皂苷的工艺条件及参数。方法以三七总皂苷的含量为考察指标,研究三七总皂苷纯化过程中,D-101型大孔吸附树脂吸附容量、洗脱溶媒及其用量,并用正交试验法优选最佳洗脱条件。结果通过D-101型大孔吸附树脂纯化后,70%乙醇洗脱液干燥后总固物中三七总皂苷纯度可达90%。结论本纯化工艺设计科学合理、成本低、污染少,可作为纯化三七总皂苷成分生产工艺的依据。

Optimization of Preparative Separation and Purification of Total Polyphenols from Sargassum tenerrimum by Column Chromatography

Polyphenols from the ethanol extracts of Sargassum tenerrimum （ST） with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents： macroporous resin, silica gel, and polyvinylpolypyrrolidone （PVPP）, were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as： with the optimal values shown in brackets, the concen- tration of extract solution （4 times diluted）, pH value （6-7）, adsorption speed （3BVh-1, bed volumes/per hour）, concentration of ethanol （80%）, elution speed （3 BV ht） and elution volume （7 BV）. The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.

Separation and Purification Process of Total Flavones from Litchi Pericarp by Macroporous Absorption Resin

[ Objective] This study aimed to optimize the conditions for purification of total flavones from litchi pericarp by macroporous absorption resin. E Method] The flavones adsorption rates and desorption rates of macroporous absorption resins （AB-8, HPD-600, D101 ） were compared, and the technological parameters of D101 during the purification process were investigated. E Result] D101 macroporous absorption resin was ap- propriate for the purification of total flavonoids from litchi pericarp. The optimal technological conditions were selected .. the pH of sample solution was 5.0; concentration of sample solution was 4 mg/ml, with a volume of 2.5BV; 80% ethanol was used as elution solution, with a volume of 2.0BV. [ Condusion] The content of total flavones achieved 83% after separation by D101 macoporous absorption resin.

Research on the Separation and Purification of Cordyceps sinensis polysaccharides by Macro porous Adsorption Resin

In this study, the separation and purification of Cordyceps sinensis polysaccharides by AB-8 and D-280 macro porous ad- sorption resin were researched, the adsorption characters when AB-8 macro porous adsorption resin processed Cordyceps sinensis leachate were determined, and the eluent were bleaching treated by D-280 macro porous adsorption resin. The results showed that the concentration of 2.0mg/mL, pH = 6 at a flow rate of 0.8mL/min. The yielding amount of polysaccharides is 246.4mg, and after decolouring treatment, the final yield is 207.1 mg.

红花提取物纯化过程的近红外光谱快速测定方法研究

目的:应用近红外光谱法,实现中药提取物大孔树脂纯化过程中质量控制指标含量的快速检测和吸附终点的快速判断。方法:本研究以红花提取物大孔树脂吸附过程为例,将近红外光谱在线分析技术与偏最小二乘法(PLS)、移动块标准偏差法(MBSD)等方法相结合,以HPLC为参照,构建红花提取物大孔树脂吸附过程的羟基红花黄色素A(HSYA)定量校正模型和吸附终点的定性检测方法。结果:所建立的HSYA定量校正模型的相关系数R达到0.999,校正集RPD和验证集RPD分别为5.54,5.22(RPD>5),模型定量效果良好;同时利用吸附过程采集的近红外光谱,通过MBSD计算和HPLC所获得的吸附终点判断结果接近(仅有1 min偏差)。结论:该法操作简便、快速无损,能够应用于红花提取物大孔树脂纯化过程中HSYA浓度的快速检测和吸附终点的快速判断。