The distribution of ^(125)I recombinant E. coli L-asparaginase in tissues ororgans and the excretion in urine, feces and bile were studied with in vivo radioactive tracertechnique. The amount of radioactivity excreted...The distribution of ^(125)I recombinant E. coli L-asparaginase in tissues ororgans and the excretion in urine, feces and bile were studied with in vivo radioactive tracertechnique. The amount of radioactivity excreted in urine, feces and bile within 24 h afterintravenous administration of ^(125)I recombinant E. col L-asparaginase to rats was 68.95% ,4.44%and 5.36% of the dose respectively. ^(125)I recombinant E. coli L-asparaginase in plasma samples wasdetermined. The levels of structural intact molecule in plasma samples were evaluated by SDS-PAGEand bio-imaging analyzer system. Pharmacokinetic parameters were assessed with a model-dependentmethod. The concentration-time curves of recombinant E. coli L-asparaginase after intravenousinjection at 1 250 IU·kg^(-1), 2 500, IU·kg^(-1), 5 000 IU·kg^(-1) to rats were consistent withthe two-compartment model. The first and terminal elimination t_(1/2) were 0.52 ~ 0.63 h and 2.39 ~2.76 h respectively. AUC was linearly related to the doses. The results of distribution in tissuesor organs and excretion in urine suggested that the metabolites of the enzyme were cleared bymechanisms of urinary excretion. Pharmacokinetics parameters of recombinant E. coli L- asparaginasein rats are warranted for the design of future clinical trials.展开更多
Chinese universities are facing a series of challenges in their accommodations, of which a predominant one is how to provide both students and staff with enough accommodations of a large scope and good quality in good...Chinese universities are facing a series of challenges in their accommodations, of which a predominant one is how to provide both students and staff with enough accommodations of a large scope and good quality in good time and with limited budgets. In order to find applicable approaches to solve this problem, the current situations of the Chinese higher education reform and the resulted rapid growth of student numbers whereby the requirement of lodgings is greatly increased are discussed. It is elucidated that in providing enough accommodations for staff and students, Chinese universities are confronted with difficulties including demand too big, lack of funding support, time limitation and incapability in both project management and facilities management. Industrialized building approach is demonstrated to be a desirable access to build large quantities of accommodations of good quality in limited time, and Private Financial Initiative (PFI) appears to be a prosperous solution to the fund shortage. Regarding project and facilities management, qualified professionals are available by either employing exterior consultants or training related staff of universities for such occupations.展开更多
Actinomycetes population from continental slope sediment of the Bay of Bengal was studied. Samples were collected during two voyages of FORV Sagar Sampada in 2004 （May-June） and 2005 （July） respectively from 11 tr...Actinomycetes population from continental slope sediment of the Bay of Bengal was studied. Samples were collected during two voyages of FORV Sagar Sampada in 2004 （May-June） and 2005 （July） respectively from 11 transects （each transect had ca. 200 m, 500 m, and 1 000 m depth stations）. The physicochemical parameters of overlying water, and sediment samples were also recorded. The actinomycete population ranged from 5.17 to 51.94 CFU/g dry sediment weight and 9.38 to 45.22 CFU/g dry sediment weight during the two cruises respectively. No actinomycete colony was isolated from stations in 1 000 m depth. Two-way analysis of variance showed significant variation among stations （ANOVA two-way, P〈0.05）, but no significance was found between the two cruises （ANOVA two-way, P〈0.05）. Populations in stations in 500 m depth in both cruises were higher than that of 200 m depth stations with statistically insignificant difference （ANOVA two-way, P〉0.05）. Three actinomycetes genera were identified. Streptomyces was found to be the dominating one in both the cruises, followed by Micromonospora, and Actinomyces. The spore of Streptomyces isolates showed the abundance in spiral spore chain. Spore surface was smooth. Multiple regression analysis revealed that the influencing physico-chemical factors were sediment pH, sediment temperature, TOC, porosity, salinity, and pressure. The media used in the present study was prepared with seawater. Thus, they may represent an autochthonous marine flora and deny the theory of land runoff carriage into the sea for adaptation to the salinity of the seawater and sediments.展开更多
基金ProjectsupportedbytheNationalNinth FivePlanKeyProjectFoundation No 96 90 2 0 1 2 5
文摘The distribution of ^(125)I recombinant E. coli L-asparaginase in tissues ororgans and the excretion in urine, feces and bile were studied with in vivo radioactive tracertechnique. The amount of radioactivity excreted in urine, feces and bile within 24 h afterintravenous administration of ^(125)I recombinant E. col L-asparaginase to rats was 68.95% ,4.44%and 5.36% of the dose respectively. ^(125)I recombinant E. coli L-asparaginase in plasma samples wasdetermined. The levels of structural intact molecule in plasma samples were evaluated by SDS-PAGEand bio-imaging analyzer system. Pharmacokinetic parameters were assessed with a model-dependentmethod. The concentration-time curves of recombinant E. coli L-asparaginase after intravenousinjection at 1 250 IU·kg^(-1), 2 500, IU·kg^(-1), 5 000 IU·kg^(-1) to rats were consistent withthe two-compartment model. The first and terminal elimination t_(1/2) were 0.52 ~ 0.63 h and 2.39 ~2.76 h respectively. AUC was linearly related to the doses. The results of distribution in tissuesor organs and excretion in urine suggested that the metabolites of the enzyme were cleared bymechanisms of urinary excretion. Pharmacokinetics parameters of recombinant E. coli L- asparaginasein rats are warranted for the design of future clinical trials.
文摘Chinese universities are facing a series of challenges in their accommodations, of which a predominant one is how to provide both students and staff with enough accommodations of a large scope and good quality in good time and with limited budgets. In order to find applicable approaches to solve this problem, the current situations of the Chinese higher education reform and the resulted rapid growth of student numbers whereby the requirement of lodgings is greatly increased are discussed. It is elucidated that in providing enough accommodations for staff and students, Chinese universities are confronted with difficulties including demand too big, lack of funding support, time limitation and incapability in both project management and facilities management. Industrialized building approach is demonstrated to be a desirable access to build large quantities of accommodations of good quality in limited time, and Private Financial Initiative (PFI) appears to be a prosperous solution to the fund shortage. Regarding project and facilities management, qualified professionals are available by either employing exterior consultants or training related staff of universities for such occupations.
文摘Actinomycetes population from continental slope sediment of the Bay of Bengal was studied. Samples were collected during two voyages of FORV Sagar Sampada in 2004 （May-June） and 2005 （July） respectively from 11 transects （each transect had ca. 200 m, 500 m, and 1 000 m depth stations）. The physicochemical parameters of overlying water, and sediment samples were also recorded. The actinomycete population ranged from 5.17 to 51.94 CFU/g dry sediment weight and 9.38 to 45.22 CFU/g dry sediment weight during the two cruises respectively. No actinomycete colony was isolated from stations in 1 000 m depth. Two-way analysis of variance showed significant variation among stations （ANOVA two-way, P〈0.05）, but no significance was found between the two cruises （ANOVA two-way, P〈0.05）. Populations in stations in 500 m depth in both cruises were higher than that of 200 m depth stations with statistically insignificant difference （ANOVA two-way, P〉0.05）. Three actinomycetes genera were identified. Streptomyces was found to be the dominating one in both the cruises, followed by Micromonospora, and Actinomyces. The spore of Streptomyces isolates showed the abundance in spiral spore chain. Spore surface was smooth. Multiple regression analysis revealed that the influencing physico-chemical factors were sediment pH, sediment temperature, TOC, porosity, salinity, and pressure. The media used in the present study was prepared with seawater. Thus, they may represent an autochthonous marine flora and deny the theory of land runoff carriage into the sea for adaptation to the salinity of the seawater and sediments.
