This study was conducted to establish a simple convenient method for calculating crop coefficient, and provide a certain basis for the research of the empirical formula for calculating crop coefficient with plant heig...This study was conducted to establish a simple convenient method for calculating crop coefficient, and provide a certain basis for the research of the empirical formula for calculating crop coefficient with plant height which could be measured conveniently with regional differences, especially for the establishment of accurate irrigation schedule of potato in Yunnan. By the field experiment on potato under the condition of drip irrigation, it was found that the models of plant height with corrected FAO-56-recommended K and measured K were a quartic polynomial and a cubic polynomial, respectively, and the polynomial of potato plant height with measured crop coefficient was simpler with higher degree of fitting; and the differences between the period with the highest change rate of potato plant height and the periods with the greatest FAO-56-recommended K and measured K exhibited a differences of 3 d. In conclusion: In the future study of simple or empirical formula calculation of crop coefficient, plant height should be considered as a main dependent variable in that the calculation result would be closer to the measured crop coefficient with the problem of regional difference existing in the FAO method solved and the formula might be simpler; and the irrigation time of potato should be 3 d earlier than the irrigation time determined according to the corrected FAO-56-recommended crop coefficient, especially in the key water requirement stages of potato.展开更多
Fluoride contents in plants and soils in Kaili City were measured with fluorinion as per electrode method and the related characteristics were analyzed in order to explore effects of air fluoride pollution on plant an...Fluoride contents in plants and soils in Kaili City were measured with fluorinion as per electrode method and the related characteristics were analyzed in order to explore effects of air fluoride pollution on plant and soil.The results indicated that fluoride content in plants tended to be volatile in 135.62-1 420.97 μg/g and averaged 513.99 μg/g;fluoride content in soils changed from 240.50-340.36 μg/g and averaged 279.60 μg/g.The contents of plant and soil both exceeded background value,suggesting that plants and soils in the region have been polluted.In addition,fluoride contents differ significantly upon plants.In detail,the maximal content was in Camelliaolelfera Abel and the minimal in Camelliaolelfera Abel.The contents of fluoride in different plant species vary,as follows:shrub vine herbaceous plant arbor;evergreen plants deciduous plant;fluoride contents in plants and soils also differ in varying degrees upon research sites.展开更多
The characteristics of macrofauna communities in three mangrove assemblages [Avicennia marina+Acgiceras corniculatum (MC)community, A.corniculatum (C) community and Bruguiera gymnorrhiza+A.corniculatum (GC) co...The characteristics of macrofauna communities in three mangrove assemblages [Avicennia marina+Acgiceras corniculatum (MC)community, A.corniculatum (C) community and Bruguiera gymnorrhiza+A.corniculatum (GC) community] were studied in Zhanjiang Mangrove Nature Reserves during 2005 and 2006. Of the three mangrove assemblages, the macrofauna species number, density, biomass, Richness index and Shannon-Wiener index were the highest, and the Simpson dominance index was medial at MC community. However the Pielou Evenness index of MC community was slightly lower than that at C community. At C community, the number of macrofauna species obviously reduced, especially infaunal, caving and adhering life forms, and the biomass and density were the lowest. Because of the even distribution of individuals of different species, the Simpson dominance index was the lowest and the evenness index was the highest. Although the Richness index at C community was slightly lower than that at MC community, the Shannon-Wiener index was near to that at MC community. At GC community, the number of macrofauna species, especially infaunal and caving life forms, continued to decrease comparing C community, but the biomass and density increased slightly. As the distribution of individuals of different species was uneven, the Simpson dominance index was the highest and the Pielou Evenness index was the lowest. Furthermore, the Richness index dropped to the lowest. The Shannon-Wiener index also dropped accordingly to the lowest. The dominant life forms of MC were infaunal and caving, while those of C and GC community were both caving. The ratio of the GS/GSB of macrofauna communities in the three mangrove assemblages were 0.48, 0.63 and 0.80, respectively. The community structures at the same mangrove assemblages were all quite similar, with those at GC community being most similar. However, there were obvious differences among the community structures at the three different mangrove assemblages. These results implied that the different mangrove assemblages had different affects on the macrofauna communities and shed light on the macrofauna adaptation capability to specific habitats.展开更多
[ Objective] The study was to compare the structural and moisture characteristics of leaf from the plantlets of three types of omamental lily( Lilium brownii). [ Method ] The paraffin sections of leaves of tested li...[ Objective] The study was to compare the structural and moisture characteristics of leaf from the plantlets of three types of omamental lily( Lilium brownii). [ Method ] The paraffin sections of leaves of tested lily varieties were prepared and then observed under microscope, and the stomatal characteristics and moisture characteristics of tested lily varieties were measured. I Resaltl All the three ornamental lily varieties show isobilateral leaf, single layer of epicuticula and lower epidermis, and no obvious differentiation of palisade tissue and spongy tissue; their stomata distribute in lower epidermis, and the guard cells are dumbbell-shaped; all of these matedais present high moisture. For the leaf sick- ness, midrib sickness and mesophyll tissue sickness, the order was determined to be oriental lily 〉 Lilium/ongiflorum 〉 Asian lily; of the three types of ornamental lily, Ulium Iongiflorum has the largest stomatai aperture and Asian lily has the smallest; focusing the water potential and moisture, the turn was Asian lily 〉 oriental lily 〉 Lilium Iong'fflorum. [ Condusion] The study may facilitate the artificial regulation of the growth conditions of the plantlets of ornamental lily.展开更多
After insufflating extended endophytes phomopsis B3 ferment diluent on seedling bed of rice, the effect of microbial inoculum on rice growth was studied. The investigation result of seedlings before transplanting seed...After insufflating extended endophytes phomopsis B3 ferment diluent on seedling bed of rice, the effect of microbial inoculum on rice growth was studied. The investigation result of seedlings before transplanting seedling showed that leaf age,the number of green leaves,the number of tiller of individual plant and the weight of per 100 fresh plants in microbial inoculum treatment group increased 0.11,0.12 ,0. 03 and 2.6 g respectively compared with those of control group. 20 days after transplanting, increased tiller number in microbial inoculum treatment group was 55 500 hm^2 more than that in control group. 30 days after transplanting, tiller number in microbial inoculum treatment group was 42 000 hm^2 more than that in control group. In harvest season, the yield ,weight of I 0130 grain and seed-setting rate increased 300 kg/hm2, 0. 7 g and 0. 23% respectively compared with these in control group. The result of preliminary study demonstrated that microbial inoculum confected by endophytes phomopsis B3 could increase the number of strong seedling and yield.展开更多
[Objective] The aim of this study is to investigate species diversity of alpine vegetation in different altitudes of Daban mountain. [Method] Plant communities were surveyed in three different altitudes (3 025 m,3 40...[Objective] The aim of this study is to investigate species diversity of alpine vegetation in different altitudes of Daban mountain. [Method] Plant communities were surveyed in three different altitudes (3 025 m,3 405 m,3 813 m) of Daban mountain,the eastern Qilian,and the richness or diversity index of vegetation in three different altitudes was comparatively analyzed. [Result] The species richness decreased with the gradual increase of altitude,and species diversity of plant communities was relatively lower in medium altitude. Furthermore,community similarity also decreased with the increase of altitude,and β diversity of communities had a significant change. [Conclusion] Environmental factor change caused by different altitudes and human disturbance are important reasons for the change of species distribution pattern in different altitudes.展开更多
In order to investigate a better soilless culture medium and the possibility of grass clippings as alternative substrate for peat moss to grow Begonia cucullata Willd. in pots, the seedlings of widely cultivated B. cu...In order to investigate a better soilless culture medium and the possibility of grass clippings as alternative substrate for peat moss to grow Begonia cucullata Willd. in pots, the seedlings of widely cultivated B. cucullata were grown in pots with eight types of soilless substrates: T1(peat: perlite=1:1), T2(peat: perlite: pine bark=1:1:1), T3(corn stover: perlite: pine bark=1:1:1), T4(corn stover: perlite: pine bark=2:1:1), T5(grass clipping: perlite: pine bark=1:1:1), T6(grass clipping: perlite:pine bark=2:1:1), T7(vermiculite: perlite: pine bark=1:1:1) and T8(vermiculite: perlite: pine bark=2:1:1), respectively, in a shade house of Shanghai Chenshan Botanical Garden to evaluate plant growth performance. The results showed that the stem diameter of B. cucullata increased significantly from day 30 to day 60 after potting,and the plant height, leaf number and leaf area increased largest from day 60 to day 90 after potting in all treatments. The growth of plants performed best in T6,with the highest branch number, leaf thickness, flower number, aboveground fresh and dry weights and relative chlorophyll content. Therefore, the substrate with grass clipping: perlite: pine bark=2:1:1 was the best culture medium for growing B. cucullata in this study, and the grass clippings could replace peat moss as alternative substrate for growing B. cucullata in containers.展开更多
A algal bloom process had been simulated via field mesocosm experiment, and the change of phytoplankton assemblage of different sizes in different growing phases had been studied. Nutrients addition could promote the ...A algal bloom process had been simulated via field mesocosm experiment, and the change of phytoplankton assemblage of different sizes in different growing phases had been studied. Nutrients addition could promote the growth of phytoplankton In the mesocosm of Prorocentrum donghaiense (M1) and the mesocosm of natural waters (M2), and the peaks of chlorophyll a were 112.79 mg/m and 235.60 mg/m, respectively. The restraining effect of nano-phytoplankton on pico-phytoplankton growth was stronger in M2 than in M1. When nutrients were abundant, the relative growth rate of diatom was higher than that of P. donghaiense, and they reached the peak quickly and then came to die out very fast. The decreasing of Si promoted diatom bloom to die out.展开更多
With the significant increase of the number of entry mails in China, mail inspection has become more and more challenge. Australia and New Zealand are the two countries recognized as the ones with the most stringent i...With the significant increase of the number of entry mails in China, mail inspection has become more and more challenge. Australia and New Zealand are the two countries recognized as the ones with the most stringent inspection and quarantine measures in the world. The mail inspection work in China, Australia and New Zealand was compared on the aspects of mail quarantine system, inspection, quarantine treatment and punishment, based on which something worth learning and referring could be found and some suggestions were put forward, with the aim to provide theoretical basis for the animal and plant quarantine of entry mails.展开更多
ObjectiveThis study aimed to construct plant expression vector for recombinant human epidermal growth factor (hEGF) and further to provide a basis for the expression of hEGF in peanut hairy root system. MethodAccord...ObjectiveThis study aimed to construct plant expression vector for recombinant human epidermal growth factor (hEGF) and further to provide a basis for the expression of hEGF in peanut hairy root system. MethodAccording to the hEGF sequence in GenBank, hEGF was synthesized artificially; subsequently, hEGF gene was ligated with green fluorescent protein (GFP) gene, and their ligation product was then amplified with primers flanked with corresponding endonuclease cleavage sites, followed by double digestion by Sal I and EcoR I of the amplified products; next, pRI 101 AN DNA was extracted and digested by both Sal I and EcoR I; susequently, the digestion products of hEGF and GFP ligation fragment by Sal I and EcoR I and the digestion products of pRI 101 AN plasmid DNA by Sal I and EcoR I were ligated, and their ligation product was transformed into Escherichia coli XL10-Gold, followed by extraction of DNA from the recombinants exhibiting green fluorescence, which was then identified by enzymatic digestion and PCR, and the verified recombinant plasmid DNA was named pBZG101. ResultHuman epidermal growth factor gene (hEGF) and green fluorescent protein gene (GFP) were successfully ligated, and their ligation fragment was successfully ligated to pRI 101 AN DNA, finally with the acquirement of the plant expression vector for recombinant human epidermal growth factor-(pBZG101). ConclusionThe plant expression vector for recombinant human epidermal growth factor-(pBZG101)- was successfully constructed in this study.展开更多
基金Supported by the Scientific Research Project of Yunnan Agricultural University(A3007680)the Fund for Scientific Research of Department of Education+1 种基金Yunnan(2014y1902014y193)~~
文摘This study was conducted to establish a simple convenient method for calculating crop coefficient, and provide a certain basis for the research of the empirical formula for calculating crop coefficient with plant height which could be measured conveniently with regional differences, especially for the establishment of accurate irrigation schedule of potato in Yunnan. By the field experiment on potato under the condition of drip irrigation, it was found that the models of plant height with corrected FAO-56-recommended K and measured K were a quartic polynomial and a cubic polynomial, respectively, and the polynomial of potato plant height with measured crop coefficient was simpler with higher degree of fitting; and the differences between the period with the highest change rate of potato plant height and the periods with the greatest FAO-56-recommended K and measured K exhibited a differences of 3 d. In conclusion: In the future study of simple or empirical formula calculation of crop coefficient, plant height should be considered as a main dependent variable in that the calculation result would be closer to the measured crop coefficient with the problem of regional difference existing in the FAO method solved and the formula might be simpler; and the irrigation time of potato should be 3 d earlier than the irrigation time determined according to the corrected FAO-56-recommended crop coefficient, especially in the key water requirement stages of potato.
基金Supported by National Natural Science Foundation of China(31100187)Guizhou Science and Technology Foundation([2008]2030)Natural Science Foundation of Guizhou Provincial Education Department(2007054)~~
文摘Fluoride contents in plants and soils in Kaili City were measured with fluorinion as per electrode method and the related characteristics were analyzed in order to explore effects of air fluoride pollution on plant and soil.The results indicated that fluoride content in plants tended to be volatile in 135.62-1 420.97 μg/g and averaged 513.99 μg/g;fluoride content in soils changed from 240.50-340.36 μg/g and averaged 279.60 μg/g.The contents of plant and soil both exceeded background value,suggesting that plants and soils in the region have been polluted.In addition,fluoride contents differ significantly upon plants.In detail,the maximal content was in Camelliaolelfera Abel and the minimal in Camelliaolelfera Abel.The contents of fluoride in different plant species vary,as follows:shrub vine herbaceous plant arbor;evergreen plants deciduous plant;fluoride contents in plants and soils also differ in varying degrees upon research sites.
文摘The characteristics of macrofauna communities in three mangrove assemblages [Avicennia marina+Acgiceras corniculatum (MC)community, A.corniculatum (C) community and Bruguiera gymnorrhiza+A.corniculatum (GC) community] were studied in Zhanjiang Mangrove Nature Reserves during 2005 and 2006. Of the three mangrove assemblages, the macrofauna species number, density, biomass, Richness index and Shannon-Wiener index were the highest, and the Simpson dominance index was medial at MC community. However the Pielou Evenness index of MC community was slightly lower than that at C community. At C community, the number of macrofauna species obviously reduced, especially infaunal, caving and adhering life forms, and the biomass and density were the lowest. Because of the even distribution of individuals of different species, the Simpson dominance index was the lowest and the evenness index was the highest. Although the Richness index at C community was slightly lower than that at MC community, the Shannon-Wiener index was near to that at MC community. At GC community, the number of macrofauna species, especially infaunal and caving life forms, continued to decrease comparing C community, but the biomass and density increased slightly. As the distribution of individuals of different species was uneven, the Simpson dominance index was the highest and the Pielou Evenness index was the lowest. Furthermore, the Richness index dropped to the lowest. The Shannon-Wiener index also dropped accordingly to the lowest. The dominant life forms of MC were infaunal and caving, while those of C and GC community were both caving. The ratio of the GS/GSB of macrofauna communities in the three mangrove assemblages were 0.48, 0.63 and 0.80, respectively. The community structures at the same mangrove assemblages were all quite similar, with those at GC community being most similar. However, there were obvious differences among the community structures at the three different mangrove assemblages. These results implied that the different mangrove assemblages had different affects on the macrofauna communities and shed light on the macrofauna adaptation capability to specific habitats.
文摘[ Objective] The study was to compare the structural and moisture characteristics of leaf from the plantlets of three types of omamental lily( Lilium brownii). [ Method ] The paraffin sections of leaves of tested lily varieties were prepared and then observed under microscope, and the stomatal characteristics and moisture characteristics of tested lily varieties were measured. I Resaltl All the three ornamental lily varieties show isobilateral leaf, single layer of epicuticula and lower epidermis, and no obvious differentiation of palisade tissue and spongy tissue; their stomata distribute in lower epidermis, and the guard cells are dumbbell-shaped; all of these matedais present high moisture. For the leaf sick- ness, midrib sickness and mesophyll tissue sickness, the order was determined to be oriental lily 〉 Lilium/ongiflorum 〉 Asian lily; of the three types of ornamental lily, Ulium Iongiflorum has the largest stomatai aperture and Asian lily has the smallest; focusing the water potential and moisture, the turn was Asian lily 〉 oriental lily 〉 Lilium Iong'fflorum. [ Condusion] The study may facilitate the artificial regulation of the growth conditions of the plantlets of ornamental lily.
基金Supported by the National Natural Science Foundation of China(30770073,30500066)~~
文摘After insufflating extended endophytes phomopsis B3 ferment diluent on seedling bed of rice, the effect of microbial inoculum on rice growth was studied. The investigation result of seedlings before transplanting seedling showed that leaf age,the number of green leaves,the number of tiller of individual plant and the weight of per 100 fresh plants in microbial inoculum treatment group increased 0.11,0.12 ,0. 03 and 2.6 g respectively compared with those of control group. 20 days after transplanting, increased tiller number in microbial inoculum treatment group was 55 500 hm^2 more than that in control group. 30 days after transplanting, tiller number in microbial inoculum treatment group was 42 000 hm^2 more than that in control group. In harvest season, the yield ,weight of I 0130 grain and seed-setting rate increased 300 kg/hm2, 0. 7 g and 0. 23% respectively compared with these in control group. The result of preliminary study demonstrated that microbial inoculum confected by endophytes phomopsis B3 could increase the number of strong seedling and yield.
基金Supported by National Sci-tech Support Plan (2007BAC03A08-5)National Natural Science Foundation ( N0. 30570300, 30590381-02)The Third Period of 211 Project of Innovation Personnel Training for Postgraduate Education in Minzu University of China(0212110309090209)~~
文摘[Objective] The aim of this study is to investigate species diversity of alpine vegetation in different altitudes of Daban mountain. [Method] Plant communities were surveyed in three different altitudes (3 025 m,3 405 m,3 813 m) of Daban mountain,the eastern Qilian,and the richness or diversity index of vegetation in three different altitudes was comparatively analyzed. [Result] The species richness decreased with the gradual increase of altitude,and species diversity of plant communities was relatively lower in medium altitude. Furthermore,community similarity also decreased with the increase of altitude,and β diversity of communities had a significant change. [Conclusion] Environmental factor change caused by different altitudes and human disturbance are important reasons for the change of species distribution pattern in different altitudes.
基金Supported by the Science and Technology Project of Shanghai Municipal Greening and City Appearance Administration(F112421)Foundation of Science and Technology Commission of Shanghai Municipality(14DZ2260400)Environmental Protection Science and Technology Project of Hunan Province(XCJZ[2012]347,XCJZ[2013]229)~~
文摘In order to investigate a better soilless culture medium and the possibility of grass clippings as alternative substrate for peat moss to grow Begonia cucullata Willd. in pots, the seedlings of widely cultivated B. cucullata were grown in pots with eight types of soilless substrates: T1(peat: perlite=1:1), T2(peat: perlite: pine bark=1:1:1), T3(corn stover: perlite: pine bark=1:1:1), T4(corn stover: perlite: pine bark=2:1:1), T5(grass clipping: perlite: pine bark=1:1:1), T6(grass clipping: perlite:pine bark=2:1:1), T7(vermiculite: perlite: pine bark=1:1:1) and T8(vermiculite: perlite: pine bark=2:1:1), respectively, in a shade house of Shanghai Chenshan Botanical Garden to evaluate plant growth performance. The results showed that the stem diameter of B. cucullata increased significantly from day 30 to day 60 after potting,and the plant height, leaf number and leaf area increased largest from day 60 to day 90 after potting in all treatments. The growth of plants performed best in T6,with the highest branch number, leaf thickness, flower number, aboveground fresh and dry weights and relative chlorophyll content. Therefore, the substrate with grass clipping: perlite: pine bark=2:1:1 was the best culture medium for growing B. cucullata in this study, and the grass clippings could replace peat moss as alternative substrate for growing B. cucullata in containers.
基金National‘973’Program(2001CB409707)the Natural Science Foundation ofChina(No.40206016 and U0633007)+1 种基金the Key Program of National Natural Science Foundation of China(No.40531006)the Field Platform&Station Foundation of CAS.
文摘A algal bloom process had been simulated via field mesocosm experiment, and the change of phytoplankton assemblage of different sizes in different growing phases had been studied. Nutrients addition could promote the growth of phytoplankton In the mesocosm of Prorocentrum donghaiense (M1) and the mesocosm of natural waters (M2), and the peaks of chlorophyll a were 112.79 mg/m and 235.60 mg/m, respectively. The restraining effect of nano-phytoplankton on pico-phytoplankton growth was stronger in M2 than in M1. When nutrients were abundant, the relative growth rate of diatom was higher than that of P. donghaiense, and they reached the peak quickly and then came to die out very fast. The decreasing of Si promoted diatom bloom to die out.
文摘With the significant increase of the number of entry mails in China, mail inspection has become more and more challenge. Australia and New Zealand are the two countries recognized as the ones with the most stringent inspection and quarantine measures in the world. The mail inspection work in China, Australia and New Zealand was compared on the aspects of mail quarantine system, inspection, quarantine treatment and punishment, based on which something worth learning and referring could be found and some suggestions were put forward, with the aim to provide theoretical basis for the animal and plant quarantine of entry mails.
基金Supported by the Shangdong Natural Science Foundation(ZR2010HQ054)the Ministry of Agriculture Opening Project Fund of Key Laboratory of Rubber Biology/State Key Laboratory Breeding Base of Cultivation&Physiology for Tropical Crops(KLOF1106)the Special Fund for Backbone Teachers and Domestic Visiting Scholars of Shandong Higher Education Institutions9~~
文摘ObjectiveThis study aimed to construct plant expression vector for recombinant human epidermal growth factor (hEGF) and further to provide a basis for the expression of hEGF in peanut hairy root system. MethodAccording to the hEGF sequence in GenBank, hEGF was synthesized artificially; subsequently, hEGF gene was ligated with green fluorescent protein (GFP) gene, and their ligation product was then amplified with primers flanked with corresponding endonuclease cleavage sites, followed by double digestion by Sal I and EcoR I of the amplified products; next, pRI 101 AN DNA was extracted and digested by both Sal I and EcoR I; susequently, the digestion products of hEGF and GFP ligation fragment by Sal I and EcoR I and the digestion products of pRI 101 AN plasmid DNA by Sal I and EcoR I were ligated, and their ligation product was transformed into Escherichia coli XL10-Gold, followed by extraction of DNA from the recombinants exhibiting green fluorescence, which was then identified by enzymatic digestion and PCR, and the verified recombinant plasmid DNA was named pBZG101. ResultHuman epidermal growth factor gene (hEGF) and green fluorescent protein gene (GFP) were successfully ligated, and their ligation fragment was successfully ligated to pRI 101 AN DNA, finally with the acquirement of the plant expression vector for recombinant human epidermal growth factor-(pBZG101). ConclusionThe plant expression vector for recombinant human epidermal growth factor-(pBZG101)- was successfully constructed in this study.