[Objective] The paper aimed to provide the theoretical foundation for rational using the large-seed rice.[Method] Large-seed rice 31C122、31C125 ( nearly 50 g /1 000-weight) and normal rice varieties including Jiyuj...[Objective] The paper aimed to provide the theoretical foundation for rational using the large-seed rice.[Method] Large-seed rice 31C122、31C125 ( nearly 50 g /1 000-weight) and normal rice varieties including Jiyujing and Fuyu 333 as materials,the accumulative process for sugar and starch in seeds was compared and analyzed.[Result] Sugar content of inferior grains was higher than superior grains in all tested materials,sugar content of large seeds was lower than conventional ones;starch content of superior grains was higher than inferior grains,starch content of conventional ones was higher than large-seed rice,amylopectin content was higher than amylose content in all tested materials,amylose content of large-seed rice was lower than conventional ones;the peak of accumulation starch for superior grains of large-seed rice was later than conventional ones,inferior grains advanced;active saving period of starch for superior grains of large-seed rice was longer than conventional ones.[Conclusion] The quality of large-seed rice was better than conventional ones.展开更多
In order to provide a new utilization method for cadmium-contaminated rice,a preliminary study of the removal of rice starch cadmium by water,calcium hydroxide,sodium hydroxide and citric acid was conducted,the damage...In order to provide a new utilization method for cadmium-contaminated rice,a preliminary study of the removal of rice starch cadmium by water,calcium hydroxide,sodium hydroxide and citric acid was conducted,the damage degree of starch was compared by SEM,and then the recombinant rice was obtained by a twin-screw extruder using the starch which met the safety standard.The physical and chemical properties of recombinant rice were analyzed by acid hydrolysis and texture analyzer.The results showed that citric acid was a good rice cadmium removal agent.The quality of recombinant rice prepared from cadmium-removed rice starch was improved;the acid resistance was increased.Heavy metal contaminated rice can be a staple food with better quality and higher value through processing.展开更多
A limited number of studies have been conducted on the effects of ozone use on cereal and its components. Starch, an important natural macromolecule, is an essential component of cereals. In present study, the effects...A limited number of studies have been conducted on the effects of ozone use on cereal and its components. Starch, an important natural macromolecule, is an essential component of cereals. In present study, the effects of ozone on the structure, physico-chemical and microbiological properties of wheat, corn, potato and rice starches were investigated. Wheat starch, corn starch, potato starch and rice starch water mixtures were prepared in the ratio of 1:9 (dry sample to water ratio) and treated with ozone at a rate of 60 g/h for 1 hour, while unozonated samples were used as controls. Light microscopy represented that the structures of corn and potato starch samples were affected by 1 hr ozonation rather than the structures of wheat and rice starch samples. Colour of ozone-oxidized wheat, corn and potato starch samples could meet the consumer preference due to the higher lightness, higher whiteness and lower chroma value. However, high chroma, high yellownes and low whiteness values of ozonated rice starch is not adequate for consumer desire. Microbiological analysis showed that 1 hr ozonation reduced the total bacteria and mould/yeast counts significantly for all wheat, corn, potato and rice starch samples (P 〈 0.05) due to most probably the reduced pH of samples. Ozonation can be an alternative modification technique, but further research is needed to understand the certain benefits and limitations of ozone applications on starch oxidation for human safety and health.展开更多
A 12-week feeding trial was conducted to evaluate the effects of dietary stachyose on the growth performance, digestive enzymes activities and intestinal structures of juvenile turbot(Scophthalmus maximus L). Five iso...A 12-week feeding trial was conducted to evaluate the effects of dietary stachyose on the growth performance, digestive enzymes activities and intestinal structures of juvenile turbot(Scophthalmus maximus L). Five isonitrogenous(49.58% crude protein) and isolipidic(10.50% crude lipid) diets were formulated to contain 0(Control), 0.625%(S-0.625), 1.25%(S-1.25), 2.5%(S-2.5) and 5%(S-5) stachyose, respectively. With the increase of stachyose level, the growth performance and feed utilization of turbot, such as the specific growth rate, final mean body weight, weight gain rate and feed efficiency, increased significantly(P < 0.05) and then stabilized. The feed intake of fish fed S-5 was significantly higher(P < 0.05) than that of fish in other groups. The activities of trypsin, intestinal caseinolytic, stomach and intestinal amylase were significantly influenced by stachyose(P < 0.05). The highest values of trypsin and intestinal caseinolytic activities were observed in group S-1.25, while the highest activity of stomach amylase and the lowest activity of intestine amylase were observed in group S-5. No lesion or damage was found on the distal intestine structures of fish from all treatments, while the height of simple folds in the distal intestine was significantly increased(P< 0.05) when 1.25% or 2.5% stachyose was added in the diets. These results indicated that moderate level of stachyose(1.25%) improves the growth performance, feed utilization, digestive enzyme activities and the distal intestine structures of juvenile turbot.展开更多
The seasonal mean atmospheric precipitable water and water vapor transport over the Haihe River Basin (HRB) in North China with a focus on their interannual to interdecadal variability, and then the relationships of...The seasonal mean atmospheric precipitable water and water vapor transport over the Haihe River Basin (HRB) in North China with a focus on their interannual to interdecadal variability, and then the relationships of the interannual and interdecadal variability of the water cycle over the HRB to the Pacific Decadal Oscillation (PDO) and E1 Nino-Southern Oscillation (ENSO) phenomena were investigated using the observational and National Centers for Environmental Prediction (NCEP) reanalysis data. There was a strong interdecadal variability for the water cycle (such as precipitation and water vapor transport) over the region, with an abrupt change occurring mostly in the mid 1970s. The intensity of the East Asian summer monsoon largely affected the atmospheric water vapor transport. Generally, the net meridional convergence of the water vapor flux over the region was relatively large before 1965, and it declined gradually from then on with a further notable decrease since mid 1970s. Zonal water vapor transport was similar to meridional, but with a much smaller magnitude and no noteworthy turning in the mid 1970s. Results also suggested that the wind field played an important role in the water vapor transport over the HRB before the mid 1960s, and the interdecadal variability of the water cycle (precipitation, water vapor transport, etc.) in the summer was related to the PDO; however, interannual variation of the water vapor transport could also be related to the ENSO phenomena.展开更多
This paper gives an impetus in quantifying digestible carbohydrates, which are important components of cereals, legumes and vegetables. The HPLC method was applied for the first time in India to estimate the digestibl...This paper gives an impetus in quantifying digestible carbohydrates, which are important components of cereals, legumes and vegetables. The HPLC method was applied for the first time in India to estimate the digestible carbohydrates such as sugars and starches in different branded rice, legumes and vegetable samples which were procured from local market of twin cities of Hyderabad and Secunderabad in Andhra Pradesh State, India. In the present study, we estimated carbohydrates in vitro, mimicking the in vivo condition by incorporating enzymatic digestion. Among the rice varieties the analyzed total soluble sugars were in the range of 5.69% to 9.62%, vegetables 0.0% to 3.72% and legumes 0.04% to 0.98%. Soluble starches in rice samples ranged from 12.51% to 17.64%, in vegetables from 1.73% to 7.28% and in legumes from 23.76% to 38.71%. Insoluble starches in rice samples were observed to be bracketed in between 52.53% to 60.43%, where as in vegetables from 0.58% to 8.83% and in legumes from 16.13% to 29.22%. Concluding our observation, the total amount of starches and total sugars in rice fell in between 74.32% to 80.75%, in vegetables from 1.74% to 16.11%, and in legumes from 39.93% to 68.91%.展开更多
The authors described a novel submerged batch culture system that produced high levels of amylase by Aspergillus kawachii using whole barley (WB), the surface of which is covered by its husk. In this study, detailed...The authors described a novel submerged batch culture system that produced high levels of amylase by Aspergillus kawachii using whole barley (WB), the surface of which is covered by its husk. In this study, detailed analyses determining the amylase activities, residual sugars, fungal morphology and expression levels of genes were performed in a submerged culture using WB to address the mechanism underlying high amylase productivity in A. kawachii. High levels of glucoamylase and acid-stable u-amylase were produced in this culture, and expression levels of amylases, as well as glucose-repressive genes including high-affinity glucose transporter and peroxidase/catalase were also high. On the other hand, the morphology of mycelia was altered, with swollen, bulbous, multi-septum hyphae and conidiophores that normally form in a solid culture being partially generated. Furthermore, cell cycle and post-translational modification-related gene expression levels were altered, and were similar to those in the solid culture. These findings suggest that high amylase productivity in the submerged culture using WB is accompanied by both the up-regulation of amylase genes and activation of post-translational modifications due to fungal morphological changes being brought closer to those in the solid culture.展开更多
The primary interest to this study was to investigate the effect of milling parameters on the size of hydrophobically modified starch particles, aiming to produce small, uniformly sized modified starch microspheres. O...The primary interest to this study was to investigate the effect of milling parameters on the size of hydrophobically modified starch particles, aiming to produce small, uniformly sized modified starch microspheres. Octie, a commercial product originated from cornstarch modified using Octenyl Succinate Anhydride (OSA), was dispersed (3 wt%) using different media (water or ethanol) and subsequently wet-milled using a beads mill with zirconium beads at a rotation of 6,000 rpm up to 30 min. It was found that milling Octie in water dispersion for 3 min resulted in the smallest mean particle size (2.04 i 0.91 ktm), compared to unmilled modified starch granules (15.2 ~ 6.0 lam). Granular size and morphology changed considerably with further milling. For instance, very dense clusters with variable particle sizes (20.6 ~ 10.0 lam) were obtained after 30 min milling. As depicted by Scanning Electronic Microscopy, a large number of particles were apparently flattened during the milling process rather than broken, forming aggregates. Ultimately, within the range of experimental conditions tested, production of sub-micron modified starch particles was not possible.展开更多
Cardiac amyloidoses (CA), a disease caused by the pre- cipitation of amyloid proteins in myocardial extracellular matrix, is difficult to diagnose due to its lack of specific clinical manifestations, and can be easi...Cardiac amyloidoses (CA), a disease caused by the pre- cipitation of amyloid proteins in myocardial extracellular matrix, is difficult to diagnose due to its lack of specific clinical manifestations, and can be easily misdiagnosed.[1] However, due to the recent improved awareness and wide use of biopsy, most patients are now correctly diagnosed prior to death. This report concerns a 59-year-old male who presented with symptoms of double eyelid swelling and chest tightness without obvious causes.展开更多
The mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis occupy the intertidal zone.However,both species have their own unique diet.The former is an herbivore and the latter is a ca...The mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis occupy the intertidal zone.However,both species have their own unique diet.The former is an herbivore and the latter is a carnivore.In order to reveal the relationship between digestive enzyme activities and diets in the two species,the activities of protease(P),non-specific bile salt-activated lipase(BAL) and α-amylase(A) were determined in the stomach and intestine of adult mudskipper B.pectinirostris and Chinese black sleeper B.sinensis.The results showed that the activities of protease,BAL and α-amylase in the intestine of B.pectinirostris were significantly(P<0.05) higher than those in the stomach.In B.sinensis,gastric protease activity was not different from the intestinal protease(P>0.05),while BAL and α-amylase activities of the intestine were significantly(P<0.05) higher than those of the stomach.The activity of gastric protease in B.sinensis was significantly(P<0.05) higher than that in B.pectinirostris,while the activities of intestinal protease were not different between the two fish species(P>0.05).BAL activities of the stomach and intestine in B.sinensis were significantly(P<0.05) higher than those in B.pectinirostris,while α-amylase activities of the stomach and intestine in B.pectinirostris were significantly(P<0.05) higher than those in B.sinensis.The ratios of P/BAL,A/P and A/BAL of the digestive tract in B.pectinirostris were 1.5,107.3 and 158.6,respectively;and those in B.sinensis were 0.2,1.6 and 0.2,respectively.It can be concluded that food digestion in the adult B.pectinirostris is mainly carried out in the intestine,whereas in the adult B.sinensis it is initiated in the stomach and finishes in the intestine.The activities of BAL and α-amylase in B.pectinirostris and B.sinensis are well correlated with their diets.However,a clear-cut correlation between protease activity and diets is not found in these two species.展开更多
Gibbsite is the usual precipitation product from alumina refineries with either Bayer or sintering process.However,the advantage of boehmite precipitation over gibbsite precipitation is the significant energy saving i...Gibbsite is the usual precipitation product from alumina refineries with either Bayer or sintering process.However,the advantage of boehmite precipitation over gibbsite precipitation is the significant energy saving in the subsequent calcination step.The current investigation takes a pragmatic approach to measure precipitation ratios,determine product phase,morphology and particle size distribution,and assess the impacts and adjustment capability of main parameters such as seed,temperature,ethanol medium,and supersaturation on the precipitation kinetics and alumina hydrate type during co-precipitation process.The results clarify that gibbsite and boehmite both can be precipitated from supersaturated sodium aluminate solutions simultaneously,and the competitive formation between Al(OH)3 and γ-AlOOH determines the main precipitate phases from pregnant liquor.Boehmite seeds,high temperature and ethanol addition can promote the boehmite precipitation and improve the mass fraction of boehmite in products.Co-precipitation changes the multimodal distribution of seeds to a normal and well distribution of products,and the particle size is more than several times that of seeds.展开更多
Geniposide is a major bioactive constituent isolated from Gardeniajasminoides Ellis. To evaluate the pharmacokinetics of geniposide in pre-clinical studies, a rapid and specific liquid chromatography-tandem mass spect...Geniposide is a major bioactive constituent isolated from Gardeniajasminoides Ellis. To evaluate the pharmacokinetics of geniposide in pre-clinical studies, a rapid and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated. After simple protein precipitation, geniposide was analyzed on a DiamonsilR C18 column with a mobile phase of 10 mM ammonium acetate and methanol (20:80, v/v) at a flow rate of 0.6 mL/min. Detection was performed in "Truncated" multiple-reaction monitoring (MRM) mode with positive electrospray ionization (ESI) at m/z 411→411 for geniposide, and MRM mode with negative ESI ionization at m/z 415→295 for puerarin (internal standard, IS). Linearity was established in the concentration range from 10.0 to 5000 ng/mL. The extraction recoveries ranged from 84.8% to 90.5% at concentrations of 10.0, 500 and 4.5x 103 ng/mL. The lower limit of quantification (LLOQ) was 10.0 ng/mL with 50 ~tL plasma. The validated method was successfully applied to the pharmacokinetic study of geniposide in rats at a dose of 200 mg/kg by oral administration.展开更多
13-Amylase activity (BAA) and thermostability (BAT) are important traits for malt quality. In this study, 138 Tibetan annual wild barley accessions and 20 cultivated genotypes differing in BAh, were planted and an...13-Amylase activity (BAA) and thermostability (BAT) are important traits for malt quality. In this study, 138 Tibetan annual wild barley accessions and 20 cultivated genotypes differing in BAh, were planted and analyzed in 2009 and 2012. Significant differences were detected among genotypes in BAA and BAT. The cultivated genotypes had a mean BAA of 1137.6 U/g and a range of from 602.1 to 1407.5 U/g, while the wild accessions had a mean of 1517.9 U/g and a range of from 829.7 to 2310.0 U/g. The cultivated genotypes had a mean relative residual 13-amylase activity (RRBAA) of 61.6% and a range of from 22.2% to 82.3%, while the wild barleys had a mean of 57.8% and a range of from 21.9% to 96.1%. Moreover, there was a significant difference among genotypes in the response of RRBAA to the temperature and duration of heat treatment. The wild barleys had wider variation in BAA and BAT than cultivated genotvDes.展开更多
基金Supported by Sub-topic of National Science and Technology Support Program(2006BAD13B01-22)~~
文摘[Objective] The paper aimed to provide the theoretical foundation for rational using the large-seed rice.[Method] Large-seed rice 31C122、31C125 ( nearly 50 g /1 000-weight) and normal rice varieties including Jiyujing and Fuyu 333 as materials,the accumulative process for sugar and starch in seeds was compared and analyzed.[Result] Sugar content of inferior grains was higher than superior grains in all tested materials,sugar content of large seeds was lower than conventional ones;starch content of superior grains was higher than inferior grains,starch content of conventional ones was higher than large-seed rice,amylopectin content was higher than amylose content in all tested materials,amylose content of large-seed rice was lower than conventional ones;the peak of accumulation starch for superior grains of large-seed rice was later than conventional ones,inferior grains advanced;active saving period of starch for superior grains of large-seed rice was longer than conventional ones.[Conclusion] The quality of large-seed rice was better than conventional ones.
文摘In order to provide a new utilization method for cadmium-contaminated rice,a preliminary study of the removal of rice starch cadmium by water,calcium hydroxide,sodium hydroxide and citric acid was conducted,the damage degree of starch was compared by SEM,and then the recombinant rice was obtained by a twin-screw extruder using the starch which met the safety standard.The physical and chemical properties of recombinant rice were analyzed by acid hydrolysis and texture analyzer.The results showed that citric acid was a good rice cadmium removal agent.The quality of recombinant rice prepared from cadmium-removed rice starch was improved;the acid resistance was increased.Heavy metal contaminated rice can be a staple food with better quality and higher value through processing.
文摘A limited number of studies have been conducted on the effects of ozone use on cereal and its components. Starch, an important natural macromolecule, is an essential component of cereals. In present study, the effects of ozone on the structure, physico-chemical and microbiological properties of wheat, corn, potato and rice starches were investigated. Wheat starch, corn starch, potato starch and rice starch water mixtures were prepared in the ratio of 1:9 (dry sample to water ratio) and treated with ozone at a rate of 60 g/h for 1 hour, while unozonated samples were used as controls. Light microscopy represented that the structures of corn and potato starch samples were affected by 1 hr ozonation rather than the structures of wheat and rice starch samples. Colour of ozone-oxidized wheat, corn and potato starch samples could meet the consumer preference due to the higher lightness, higher whiteness and lower chroma value. However, high chroma, high yellownes and low whiteness values of ozonated rice starch is not adequate for consumer desire. Microbiological analysis showed that 1 hr ozonation reduced the total bacteria and mould/yeast counts significantly for all wheat, corn, potato and rice starch samples (P 〈 0.05) due to most probably the reduced pH of samples. Ozonation can be an alternative modification technique, but further research is needed to understand the certain benefits and limitations of ozone applications on starch oxidation for human safety and health.
基金supported by the Research Fund for the Doctoral Program of Higher Education of China(No.20120132120025)National Program on Key Basic Re-search Project(973 Program,2014CB138600)+1 种基金the Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province(No.BS2013 SW007)Modern Agro-Industry Technology Research System(No.nycytx-50-G07)
文摘A 12-week feeding trial was conducted to evaluate the effects of dietary stachyose on the growth performance, digestive enzymes activities and intestinal structures of juvenile turbot(Scophthalmus maximus L). Five isonitrogenous(49.58% crude protein) and isolipidic(10.50% crude lipid) diets were formulated to contain 0(Control), 0.625%(S-0.625), 1.25%(S-1.25), 2.5%(S-2.5) and 5%(S-5) stachyose, respectively. With the increase of stachyose level, the growth performance and feed utilization of turbot, such as the specific growth rate, final mean body weight, weight gain rate and feed efficiency, increased significantly(P < 0.05) and then stabilized. The feed intake of fish fed S-5 was significantly higher(P < 0.05) than that of fish in other groups. The activities of trypsin, intestinal caseinolytic, stomach and intestinal amylase were significantly influenced by stachyose(P < 0.05). The highest values of trypsin and intestinal caseinolytic activities were observed in group S-1.25, while the highest activity of stomach amylase and the lowest activity of intestine amylase were observed in group S-5. No lesion or damage was found on the distal intestine structures of fish from all treatments, while the height of simple folds in the distal intestine was significantly increased(P< 0.05) when 1.25% or 2.5% stachyose was added in the diets. These results indicated that moderate level of stachyose(1.25%) improves the growth performance, feed utilization, digestive enzyme activities and the distal intestine structures of juvenile turbot.
基金the Key Knowledge Innovation Project of the Chinese Academy of Sciences (Nos. KZCX2-SW-317and KZCX3-SW-226).
文摘The seasonal mean atmospheric precipitable water and water vapor transport over the Haihe River Basin (HRB) in North China with a focus on their interannual to interdecadal variability, and then the relationships of the interannual and interdecadal variability of the water cycle over the HRB to the Pacific Decadal Oscillation (PDO) and E1 Nino-Southern Oscillation (ENSO) phenomena were investigated using the observational and National Centers for Environmental Prediction (NCEP) reanalysis data. There was a strong interdecadal variability for the water cycle (such as precipitation and water vapor transport) over the region, with an abrupt change occurring mostly in the mid 1970s. The intensity of the East Asian summer monsoon largely affected the atmospheric water vapor transport. Generally, the net meridional convergence of the water vapor flux over the region was relatively large before 1965, and it declined gradually from then on with a further notable decrease since mid 1970s. Zonal water vapor transport was similar to meridional, but with a much smaller magnitude and no noteworthy turning in the mid 1970s. Results also suggested that the wind field played an important role in the water vapor transport over the HRB before the mid 1960s, and the interdecadal variability of the water cycle (precipitation, water vapor transport, etc.) in the summer was related to the PDO; however, interannual variation of the water vapor transport could also be related to the ENSO phenomena.
文摘This paper gives an impetus in quantifying digestible carbohydrates, which are important components of cereals, legumes and vegetables. The HPLC method was applied for the first time in India to estimate the digestible carbohydrates such as sugars and starches in different branded rice, legumes and vegetable samples which were procured from local market of twin cities of Hyderabad and Secunderabad in Andhra Pradesh State, India. In the present study, we estimated carbohydrates in vitro, mimicking the in vivo condition by incorporating enzymatic digestion. Among the rice varieties the analyzed total soluble sugars were in the range of 5.69% to 9.62%, vegetables 0.0% to 3.72% and legumes 0.04% to 0.98%. Soluble starches in rice samples ranged from 12.51% to 17.64%, in vegetables from 1.73% to 7.28% and in legumes from 23.76% to 38.71%. Insoluble starches in rice samples were observed to be bracketed in between 52.53% to 60.43%, where as in vegetables from 0.58% to 8.83% and in legumes from 16.13% to 29.22%. Concluding our observation, the total amount of starches and total sugars in rice fell in between 74.32% to 80.75%, in vegetables from 1.74% to 16.11%, and in legumes from 39.93% to 68.91%.
文摘The authors described a novel submerged batch culture system that produced high levels of amylase by Aspergillus kawachii using whole barley (WB), the surface of which is covered by its husk. In this study, detailed analyses determining the amylase activities, residual sugars, fungal morphology and expression levels of genes were performed in a submerged culture using WB to address the mechanism underlying high amylase productivity in A. kawachii. High levels of glucoamylase and acid-stable u-amylase were produced in this culture, and expression levels of amylases, as well as glucose-repressive genes including high-affinity glucose transporter and peroxidase/catalase were also high. On the other hand, the morphology of mycelia was altered, with swollen, bulbous, multi-septum hyphae and conidiophores that normally form in a solid culture being partially generated. Furthermore, cell cycle and post-translational modification-related gene expression levels were altered, and were similar to those in the solid culture. These findings suggest that high amylase productivity in the submerged culture using WB is accompanied by both the up-regulation of amylase genes and activation of post-translational modifications due to fungal morphological changes being brought closer to those in the solid culture.
文摘The primary interest to this study was to investigate the effect of milling parameters on the size of hydrophobically modified starch particles, aiming to produce small, uniformly sized modified starch microspheres. Octie, a commercial product originated from cornstarch modified using Octenyl Succinate Anhydride (OSA), was dispersed (3 wt%) using different media (water or ethanol) and subsequently wet-milled using a beads mill with zirconium beads at a rotation of 6,000 rpm up to 30 min. It was found that milling Octie in water dispersion for 3 min resulted in the smallest mean particle size (2.04 i 0.91 ktm), compared to unmilled modified starch granules (15.2 ~ 6.0 lam). Granular size and morphology changed considerably with further milling. For instance, very dense clusters with variable particle sizes (20.6 ~ 10.0 lam) were obtained after 30 min milling. As depicted by Scanning Electronic Microscopy, a large number of particles were apparently flattened during the milling process rather than broken, forming aggregates. Ultimately, within the range of experimental conditions tested, production of sub-micron modified starch particles was not possible.
文摘Cardiac amyloidoses (CA), a disease caused by the pre- cipitation of amyloid proteins in myocardial extracellular matrix, is difficult to diagnose due to its lack of specific clinical manifestations, and can be easily misdiagnosed.[1] However, due to the recent improved awareness and wide use of biopsy, most patients are now correctly diagnosed prior to death. This report concerns a 59-year-old male who presented with symptoms of double eyelid swelling and chest tightness without obvious causes.
基金Supported by Natural Science Foundation of China(No.40476056)the Key Projects of Science and Technology of Fujian Province(2003,No.262004SZ01-02)
文摘The mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis occupy the intertidal zone.However,both species have their own unique diet.The former is an herbivore and the latter is a carnivore.In order to reveal the relationship between digestive enzyme activities and diets in the two species,the activities of protease(P),non-specific bile salt-activated lipase(BAL) and α-amylase(A) were determined in the stomach and intestine of adult mudskipper B.pectinirostris and Chinese black sleeper B.sinensis.The results showed that the activities of protease,BAL and α-amylase in the intestine of B.pectinirostris were significantly(P<0.05) higher than those in the stomach.In B.sinensis,gastric protease activity was not different from the intestinal protease(P>0.05),while BAL and α-amylase activities of the intestine were significantly(P<0.05) higher than those of the stomach.The activity of gastric protease in B.sinensis was significantly(P<0.05) higher than that in B.pectinirostris,while the activities of intestinal protease were not different between the two fish species(P>0.05).BAL activities of the stomach and intestine in B.sinensis were significantly(P<0.05) higher than those in B.pectinirostris,while α-amylase activities of the stomach and intestine in B.pectinirostris were significantly(P<0.05) higher than those in B.sinensis.The ratios of P/BAL,A/P and A/BAL of the digestive tract in B.pectinirostris were 1.5,107.3 and 158.6,respectively;and those in B.sinensis were 0.2,1.6 and 0.2,respectively.It can be concluded that food digestion in the adult B.pectinirostris is mainly carried out in the intestine,whereas in the adult B.sinensis it is initiated in the stomach and finishes in the intestine.The activities of BAL and α-amylase in B.pectinirostris and B.sinensis are well correlated with their diets.However,a clear-cut correlation between protease activity and diets is not found in these two species.
基金Projects(50704030) supported by the National Natural Science Foundation of ChinaProject(KGCX2-YW-321-2) supported by the Knowledge Innovation Program of the Chinese Academy of Sciences
文摘Gibbsite is the usual precipitation product from alumina refineries with either Bayer or sintering process.However,the advantage of boehmite precipitation over gibbsite precipitation is the significant energy saving in the subsequent calcination step.The current investigation takes a pragmatic approach to measure precipitation ratios,determine product phase,morphology and particle size distribution,and assess the impacts and adjustment capability of main parameters such as seed,temperature,ethanol medium,and supersaturation on the precipitation kinetics and alumina hydrate type during co-precipitation process.The results clarify that gibbsite and boehmite both can be precipitated from supersaturated sodium aluminate solutions simultaneously,and the competitive formation between Al(OH)3 and γ-AlOOH determines the main precipitate phases from pregnant liquor.Boehmite seeds,high temperature and ethanol addition can promote the boehmite precipitation and improve the mass fraction of boehmite in products.Co-precipitation changes the multimodal distribution of seeds to a normal and well distribution of products,and the particle size is more than several times that of seeds.
基金Laboratory for Rare Disease of Shandong Province
文摘Geniposide is a major bioactive constituent isolated from Gardeniajasminoides Ellis. To evaluate the pharmacokinetics of geniposide in pre-clinical studies, a rapid and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated. After simple protein precipitation, geniposide was analyzed on a DiamonsilR C18 column with a mobile phase of 10 mM ammonium acetate and methanol (20:80, v/v) at a flow rate of 0.6 mL/min. Detection was performed in "Truncated" multiple-reaction monitoring (MRM) mode with positive electrospray ionization (ESI) at m/z 411→411 for geniposide, and MRM mode with negative ESI ionization at m/z 415→295 for puerarin (internal standard, IS). Linearity was established in the concentration range from 10.0 to 5000 ng/mL. The extraction recoveries ranged from 84.8% to 90.5% at concentrations of 10.0, 500 and 4.5x 103 ng/mL. The lower limit of quantification (LLOQ) was 10.0 ng/mL with 50 ~tL plasma. The validated method was successfully applied to the pharmacokinetic study of geniposide in rats at a dose of 200 mg/kg by oral administration.
基金Project supported by the National Natural Science Foundation of China (Nos. 31330055 and 31201205), the Zhejiang Provincial Natural Science Foundation of China (No. Z3110054), the 948 Project of Ministry of Agriculture of China (No. 2012-Z25), and the China Agriculture Research System (No. CARS-05)
文摘13-Amylase activity (BAA) and thermostability (BAT) are important traits for malt quality. In this study, 138 Tibetan annual wild barley accessions and 20 cultivated genotypes differing in BAh, were planted and analyzed in 2009 and 2012. Significant differences were detected among genotypes in BAA and BAT. The cultivated genotypes had a mean BAA of 1137.6 U/g and a range of from 602.1 to 1407.5 U/g, while the wild accessions had a mean of 1517.9 U/g and a range of from 829.7 to 2310.0 U/g. The cultivated genotypes had a mean relative residual 13-amylase activity (RRBAA) of 61.6% and a range of from 22.2% to 82.3%, while the wild barleys had a mean of 57.8% and a range of from 21.9% to 96.1%. Moreover, there was a significant difference among genotypes in the response of RRBAA to the temperature and duration of heat treatment. The wild barleys had wider variation in BAA and BAT than cultivated genotvDes.