A multivalent inactivated Escherichia coli vaccine for forest musk deer by using serotypes O4,O26,and O139 with Al(OH)3 adjuvant was prepared.The vaccine did not cause any adverse reactions in forest musk deer.The i...A multivalent inactivated Escherichia coli vaccine for forest musk deer by using serotypes O4,O26,and O139 with Al(OH)3 adjuvant was prepared.The vaccine did not cause any adverse reactions in forest musk deer.The immunogenic effects of the vaccine were experimentally investigated in pregnant and young forest musk deer.The serum antibody titers of pregnant and young forest musk deer were determined by performing the micro-agglutination test.The serum antibody titers of pregnant forest musk deer were more stable from 35th to 68th d after the third vaccination,and the serum antibody titers of four pregnant forest musk deer were maintained 25,25,25,and 24 on 68th d after the third vaccination.Young forest musk deer showed serum antibody titers which were obtained due to nursing.Young forest musk deer were administered the first intramuscular vaccine injection at an age of approximately 60 days due to a fall in maternal antibody titers.The serum antibody titers of young forest musk deer were higher after the third vaccination and maintained at approximately the same level until they were 137 days old.The maternal antibodies and the antibodies produced by young forest musk deer could be helpful for protecting the young musk deer from the infections of pathogenic Escherichia coli strains(serotypes O4,O26,and O139)for 137 days after birth(during the nursing period and the period when the forest musk deer were susceptible to diseases).展开更多
Some rodent-dispersed seeds have a hard seed-coat(e.g.woody endocarp).Specific scrapes or dental marks on the hard seed-coat left by rodents when they eat these seeds can be used to identify seed predators.In this stu...Some rodent-dispersed seeds have a hard seed-coat(e.g.woody endocarp).Specific scrapes or dental marks on the hard seed-coat left by rodents when they eat these seeds can be used to identify seed predators.In this study we measured the morphological traits of endocarp-remains of seeds of wild apricot Prunus armeniaca used by Chinese white-bellied rats Niviventor confucianus and Korean field mice Apodemus peninsulae.We established their Fisher's linear discriminant functions to separate endocarp-remains between the two predators.A total of 90.0% of the endocarp-remains left by Korean field mice and 88.0% of those left by Chinese white-bellied rats were correctly classified.The overall percentage of correct classification was 89.0%.One hundred and sixty endocarp-remains of unknown what species predated them were classified using the functions.The method may allow more reliable quantitative studies of the effects of Chinese white-bellied rats and Korean field mice on seed consumption and dispersal of wild apricot and this study might be used for reference in other studies of seed predators identification on hard seeds.展开更多
The spatial structure and variation of the upwelling in the waters east and northeast of Hainan Island, China during 2000-2007 were investigated using a nested high-resolution Princeton Ocean Model (POM) forced by Qui...The spatial structure and variation of the upwelling in the waters east and northeast of Hainan Island, China during 2000-2007 were investigated using a nested high-resolution Princeton Ocean Model (POM) forced by QuikSCAT winds. The model produced good simulations of the summer upwelling and the seasonal and annual variability. Strong upwelling occurs from mid-July to mid-August with a peak east of Hainan Island associated with the southwesterly monsoon in the South China Sea. Sensitivity experiments indicated that when the local wind stress controls the variability of the upwelling, the large-scale circulation significantly enhances the upwelling northeast of Hainan Island by inducing a local upwelling and transporting cold water northeast-ward along the island's east coast. The joint effects of the local wind stress and large-scale circulation result in stronger upwelling northeast of Hainan Island. This implies that the annual variation of the upwelling northeast of Hainan Island is controlled not only by the local alongshore wind stress but also by the large-scale circulation. This result will help us investigate the decadal variation of the upwelling in this region in the future.展开更多
Turbot(Scophthalmus maximus L.) reddish body iridovirus(TRBIV) was propagated in turbot fin cells(TF cells) and inactivated as the TRBIV vaccine with its protection efficiency evaluated in this study.TF cells were cul...Turbot(Scophthalmus maximus L.) reddish body iridovirus(TRBIV) was propagated in turbot fin cells(TF cells) and inactivated as the TRBIV vaccine with its protection efficiency evaluated in this study.TF cells were cultured in 10% bovine calf serum(BCS)-containing MEM medium(pH7.0) at 22℃,in which TRBIV propagated to a titer as high as 105.6 TCID50 mL-1.The TRBIV was inactivated with 0.1% formalin and formulated with 0.5% aluminum hydroxide.The inactivated vaccine caused neither cytopathogenic effect(CPE) on TF cells nor pathogenic effect on turbots.After being administered with the vaccine twice via muscle injection,the turbot developed high-tittered TRBIV neutralizing antibodies in a dose-dependent manner.The vaccine protected the turbot from dying with an immunoprotection rate of 83.3% as was determined via subcutaneous vaccination in the laboratory and 90.5% via bath vaccination in turbot farms,respectively.The inactivated vaccine was very immunogenic,efficiently preventing tur-bot from death.It holds the potential of being applied in aquaculture.展开更多
The greatest Phanerozoic mass extinction happened at the end-Permian to earliest Triassic. About 95% species, 82% genera, and more than half families became extinct, constituting the sole macro-mass extinction in geol...The greatest Phanerozoic mass extinction happened at the end-Permian to earliest Triassic. About 95% species, 82% genera, and more than half families became extinct, constituting the sole macro-mass extinction in geological history. This event not only caused the great extinction but also destroyed the 200 Myr-long Paleozoic marine ecosystem, prompted its transition to Mesozoic ecosystem, and induced coal gap on land as well as reef gap and chert gap in ocean. The biotic crisis during the Paleozoic-Mesozoic transition was a long process of co-evolution between geospheres and biosphere. The event sequence at the Permian-Triassic boundary (PTB) reveals two-episodic pattern of rapidly deteriorating global changes and biotic mass ex- tinction and the intimate relationship between them. The severe global changes coupling multiple geospheres may have affect- ed the Pangea integration on the Earth's surface spheres, which include: the Pangea integration→enhanced mountain height and basin depth, changes of wind and ocean current systems; enhanced ocean basin depth→the greatest Phanerozoic regression at PTB, disappearance of epeiric seas and subsequent rapid transgression; the Pangea integration→thermal isolation effect of continental lithosphere and decrease of mid-ocean ridges→development of continental volcanism; two-episode volcanism causing LIPs of the Emeishan Basalt and the Siberian Trap (25%251 Ma)→global warming and mass extinction; continental aridification and replacement of monsoon system by latitudinal wind system→destruction of vegetation; enhanced weathering and CH4 emission→negative excursion of δ^13C; mantle plume→crust doming→regression; possible relation between the Illawarra magnetic reversal and the PTB extinction, and so on. Mantle plume produced the Late Permian LIPs and mantle convection may have caused the process of the Pangea integration. Subduction, delamination, and accumulation of the earth's cool lithospheric material at the "D" layer of CMB started mantle plume by heat compensation and disturbed the outer core ther- too-convection, and the latter in turn would generate the mid-Permian geomagnetic reversal. These core and mantle perturbations may have caused the Pangea integration and two successive LIPs in the Permian, and probably finally the mass extinction at the PTB.展开更多
Carbonate carbon isotope (δ^13Ccarb) has received considerable attention in the Permian-Triassic transition for its rapid negative shift coinciding with the great end-Permian mass extinction event. The mechanism ha...Carbonate carbon isotope (δ^13Ccarb) has received considerable attention in the Permian-Triassic transition for its rapid negative shift coinciding with the great end-Permian mass extinction event. The mechanism has long been debated for such a c~ δ^13Ccarb negative excursion through the end-Permian crisis and subsequent large perturbations in the entire Early Triassic. A δ^13Ccarb depth gradient is observed at the Permian-Triassic boundary sections of different water-depths, i.e., the Yangou, Meishan, and Shangsi sections, and such a large δ^13Ccarb-depth gradient near the end-Permian mass extinction horizon is believed to result from a stratified Paleotethys Ocean with widespread anoxic/euxinic deep water. The evolution of δ^13Ccarb-depth gradient com- bined with paleontological and geochemical data suggests that abundant cyanobacteria and vigorous biological pump in the immediate aftermath of the end-Permian extinction would be the main cause of the large δ^13Ccarb-depth gradient, and the enhanced continental weathering with the mass extinction on land provides a mass amount of nutriment for the flourishing cyanobacteria. Photic zone anoxia/euxinia from the onset of chemocline upward excursion might be the direct cause for the mass extinction whereas the instability of chemocline in the stratified Early Triassic ocean would be the reason for the delayed and involuted biotic recovery.展开更多
The end-Paleozoic biotic crisis is characterized by two-phase mass extinctions;the first strike,resulting in a large decline of sessile benthos in shallow marine environments,occurred at the end-Guadalupian time.In or...The end-Paleozoic biotic crisis is characterized by two-phase mass extinctions;the first strike,resulting in a large decline of sessile benthos in shallow marine environments,occurred at the end-Guadalupian time.In order to explore the mechanism of organisms' demise,detailed analyses of depositional facies,fossil record,and carbonate carbon isotopic variations were carried out on a Maokou-Wujiaping boundary succession in northwestern Sichuan,SW China.Our data reveal a negative carbon isotopic excursion across the boundary;the gradual excursion with relatively low amplitude(2.15‰) favors a long-term influx of isotopically light 12 C sourced by the Emeishan basalt trap,rather than by rapid releasing of gas hydrate.The temporal coincidence of the beginning of accelerated negative carbon isotopic excursion with onsets of sea-level fall and massive biotic demise suggests a cause-effect link between them.Intensive volcanic activity of the Emeishan trap and sea-level fall could have resulted in detrimental environmental stresses and habitat loss for organisms,particularly for those benthic dwellers,leading to their subsequent massive extinction.展开更多
The positron-electron annihilation process in(2,2)-difluoropropane molecule and the corresponding gammaray spectra are studied by quantum chemistry method.The positrophilic electrons in(2,2)-difluoropropane molecule a...The positron-electron annihilation process in(2,2)-difluoropropane molecule and the corresponding gammaray spectra are studied by quantum chemistry method.The positrophilic electrons in(2,2)-difluoropropane molecule are found for the first time.The theoretical predictions show that the outermost 2s electrons of fluoride atoms play an important role in positron-electron annihilation process of(2,2)-difluoropropane.In the present scheme,the correlation coefficient between the theoretical gamma-ray spectra and the experiments can be 99%.The present study gives an alternative annihilation model for positron-electron pair in larger molecules.展开更多
基金Supported by Youth Foundation of Education Department in Sichuan Province(07ZB060)Scientific and Technological Supporting Project in Science and Technology Bureau of Sichuan Province(2009SZ0228)~~
文摘A multivalent inactivated Escherichia coli vaccine for forest musk deer by using serotypes O4,O26,and O139 with Al(OH)3 adjuvant was prepared.The vaccine did not cause any adverse reactions in forest musk deer.The immunogenic effects of the vaccine were experimentally investigated in pregnant and young forest musk deer.The serum antibody titers of pregnant and young forest musk deer were determined by performing the micro-agglutination test.The serum antibody titers of pregnant forest musk deer were more stable from 35th to 68th d after the third vaccination,and the serum antibody titers of four pregnant forest musk deer were maintained 25,25,25,and 24 on 68th d after the third vaccination.Young forest musk deer showed serum antibody titers which were obtained due to nursing.Young forest musk deer were administered the first intramuscular vaccine injection at an age of approximately 60 days due to a fall in maternal antibody titers.The serum antibody titers of young forest musk deer were higher after the third vaccination and maintained at approximately the same level until they were 137 days old.The maternal antibodies and the antibodies produced by young forest musk deer could be helpful for protecting the young musk deer from the infections of pathogenic Escherichia coli strains(serotypes O4,O26,and O139)for 137 days after birth(during the nursing period and the period when the forest musk deer were susceptible to diseases).
基金funded by the National Natural Science Foundation of China(30800120) and the Foundation for New Teachers of Huazhong Normal University
文摘Some rodent-dispersed seeds have a hard seed-coat(e.g.woody endocarp).Specific scrapes or dental marks on the hard seed-coat left by rodents when they eat these seeds can be used to identify seed predators.In this study we measured the morphological traits of endocarp-remains of seeds of wild apricot Prunus armeniaca used by Chinese white-bellied rats Niviventor confucianus and Korean field mice Apodemus peninsulae.We established their Fisher's linear discriminant functions to separate endocarp-remains between the two predators.A total of 90.0% of the endocarp-remains left by Korean field mice and 88.0% of those left by Chinese white-bellied rats were correctly classified.The overall percentage of correct classification was 89.0%.One hundred and sixty endocarp-remains of unknown what species predated them were classified using the functions.The method may allow more reliable quantitative studies of the effects of Chinese white-bellied rats and Korean field mice on seed consumption and dispersal of wild apricot and this study might be used for reference in other studies of seed predators identification on hard seeds.
基金Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (Nos. KZCX2-YW-Q11-02, KZCX2-EW-208)the One Hundred Talent Program of Chinese Academy of Sciences+1 种基金the National Natural Science Foundation of China (No. 41076009)the Youth Frontier Science Project of the South China Sea Institute of Oceanology(No. SQ200914)
文摘The spatial structure and variation of the upwelling in the waters east and northeast of Hainan Island, China during 2000-2007 were investigated using a nested high-resolution Princeton Ocean Model (POM) forced by QuikSCAT winds. The model produced good simulations of the summer upwelling and the seasonal and annual variability. Strong upwelling occurs from mid-July to mid-August with a peak east of Hainan Island associated with the southwesterly monsoon in the South China Sea. Sensitivity experiments indicated that when the local wind stress controls the variability of the upwelling, the large-scale circulation significantly enhances the upwelling northeast of Hainan Island by inducing a local upwelling and transporting cold water northeast-ward along the island's east coast. The joint effects of the local wind stress and large-scale circulation result in stronger upwelling northeast of Hainan Island. This implies that the annual variation of the upwelling northeast of Hainan Island is controlled not only by the local alongshore wind stress but also by the large-scale circulation. This result will help us investigate the decadal variation of the upwelling in this region in the future.
基金supported by the National High Technology Research and Development Program of China(863 Program)(2006AA10A401)
文摘Turbot(Scophthalmus maximus L.) reddish body iridovirus(TRBIV) was propagated in turbot fin cells(TF cells) and inactivated as the TRBIV vaccine with its protection efficiency evaluated in this study.TF cells were cultured in 10% bovine calf serum(BCS)-containing MEM medium(pH7.0) at 22℃,in which TRBIV propagated to a titer as high as 105.6 TCID50 mL-1.The TRBIV was inactivated with 0.1% formalin and formulated with 0.5% aluminum hydroxide.The inactivated vaccine caused neither cytopathogenic effect(CPE) on TF cells nor pathogenic effect on turbots.After being administered with the vaccine twice via muscle injection,the turbot developed high-tittered TRBIV neutralizing antibodies in a dose-dependent manner.The vaccine protected the turbot from dying with an immunoprotection rate of 83.3% as was determined via subcutaneous vaccination in the laboratory and 90.5% via bath vaccination in turbot farms,respectively.The inactivated vaccine was very immunogenic,efficiently preventing tur-bot from death.It holds the potential of being applied in aquaculture.
基金supported by the National Basic Research Program of China(Grant No.2011CB808800)the 111 Project(Grant No.B08030)+1 种基金the National Natural Science Foundation of China(Grant Nos.40621002,40830212&40921062)the Fundamental Research Funds for the Central Universities(CUG130407)
文摘The greatest Phanerozoic mass extinction happened at the end-Permian to earliest Triassic. About 95% species, 82% genera, and more than half families became extinct, constituting the sole macro-mass extinction in geological history. This event not only caused the great extinction but also destroyed the 200 Myr-long Paleozoic marine ecosystem, prompted its transition to Mesozoic ecosystem, and induced coal gap on land as well as reef gap and chert gap in ocean. The biotic crisis during the Paleozoic-Mesozoic transition was a long process of co-evolution between geospheres and biosphere. The event sequence at the Permian-Triassic boundary (PTB) reveals two-episodic pattern of rapidly deteriorating global changes and biotic mass ex- tinction and the intimate relationship between them. The severe global changes coupling multiple geospheres may have affect- ed the Pangea integration on the Earth's surface spheres, which include: the Pangea integration→enhanced mountain height and basin depth, changes of wind and ocean current systems; enhanced ocean basin depth→the greatest Phanerozoic regression at PTB, disappearance of epeiric seas and subsequent rapid transgression; the Pangea integration→thermal isolation effect of continental lithosphere and decrease of mid-ocean ridges→development of continental volcanism; two-episode volcanism causing LIPs of the Emeishan Basalt and the Siberian Trap (25%251 Ma)→global warming and mass extinction; continental aridification and replacement of monsoon system by latitudinal wind system→destruction of vegetation; enhanced weathering and CH4 emission→negative excursion of δ^13C; mantle plume→crust doming→regression; possible relation between the Illawarra magnetic reversal and the PTB extinction, and so on. Mantle plume produced the Late Permian LIPs and mantle convection may have caused the process of the Pangea integration. Subduction, delamination, and accumulation of the earth's cool lithospheric material at the "D" layer of CMB started mantle plume by heat compensation and disturbed the outer core ther- too-convection, and the latter in turn would generate the mid-Permian geomagnetic reversal. These core and mantle perturbations may have caused the Pangea integration and two successive LIPs in the Permian, and probably finally the mass extinction at the PTB.
基金supported by "973 Program" (Grant No. 2011CB808800)National Natural Science Foundation of China (Grant Nos. 40830212,40921062,41172312)+2 种基金Doctoral Fund of Ministry of Education of China (Grant No. 200804910503)Fund of State Key Laboratory of Biogeology and Environmental Geology(Grant No. BGEG0802)Scientific and Technological Project of Jiangxi (Grant No. GJJ10623)
文摘Carbonate carbon isotope (δ^13Ccarb) has received considerable attention in the Permian-Triassic transition for its rapid negative shift coinciding with the great end-Permian mass extinction event. The mechanism has long been debated for such a c~ δ^13Ccarb negative excursion through the end-Permian crisis and subsequent large perturbations in the entire Early Triassic. A δ^13Ccarb depth gradient is observed at the Permian-Triassic boundary sections of different water-depths, i.e., the Yangou, Meishan, and Shangsi sections, and such a large δ^13Ccarb-depth gradient near the end-Permian mass extinction horizon is believed to result from a stratified Paleotethys Ocean with widespread anoxic/euxinic deep water. The evolution of δ^13Ccarb-depth gradient com- bined with paleontological and geochemical data suggests that abundant cyanobacteria and vigorous biological pump in the immediate aftermath of the end-Permian extinction would be the main cause of the large δ^13Ccarb-depth gradient, and the enhanced continental weathering with the mass extinction on land provides a mass amount of nutriment for the flourishing cyanobacteria. Photic zone anoxia/euxinia from the onset of chemocline upward excursion might be the direct cause for the mass extinction whereas the instability of chemocline in the stratified Early Triassic ocean would be the reason for the delayed and involuted biotic recovery.
基金supported by National Natural Science Foundation of China(Grant No. 40839907)
文摘The end-Paleozoic biotic crisis is characterized by two-phase mass extinctions;the first strike,resulting in a large decline of sessile benthos in shallow marine environments,occurred at the end-Guadalupian time.In order to explore the mechanism of organisms' demise,detailed analyses of depositional facies,fossil record,and carbonate carbon isotopic variations were carried out on a Maokou-Wujiaping boundary succession in northwestern Sichuan,SW China.Our data reveal a negative carbon isotopic excursion across the boundary;the gradual excursion with relatively low amplitude(2.15‰) favors a long-term influx of isotopically light 12 C sourced by the Emeishan basalt trap,rather than by rapid releasing of gas hydrate.The temporal coincidence of the beginning of accelerated negative carbon isotopic excursion with onsets of sea-level fall and massive biotic demise suggests a cause-effect link between them.Intensive volcanic activity of the Emeishan trap and sea-level fall could have resulted in detrimental environmental stresses and habitat loss for organisms,particularly for those benthic dwellers,leading to their subsequent massive extinction.
基金Supported by the National Natural Science Foundation of China under Grant No.11347011the Natural Science Foundation Project of Shandong Province under Grant No.ZR2011AM0102014 Technology Innovation Fund of Ludong University under Grant Nos.1d151007 and ld15l016
文摘The positron-electron annihilation process in(2,2)-difluoropropane molecule and the corresponding gammaray spectra are studied by quantum chemistry method.The positrophilic electrons in(2,2)-difluoropropane molecule are found for the first time.The theoretical predictions show that the outermost 2s electrons of fluoride atoms play an important role in positron-electron annihilation process of(2,2)-difluoropropane.In the present scheme,the correlation coefficient between the theoretical gamma-ray spectra and the experiments can be 99%.The present study gives an alternative annihilation model for positron-electron pair in larger molecules.