真三轴大物模水力压裂裂缝起裂及扩展模拟实验

目的鄂尔多斯盆地长7致密砂岩储层岩石致密、非均质性强,孔喉分选性及连通性较差。前期常规压裂工艺改造手段单一,裂缝欠发育的致密储层改造效果不佳。方法根据量纲分析法(π定理)优化了物理模拟实验相似准则设计思路,设计了等比例缩小的压裂裂缝起裂及延伸模拟系统。利用大尺寸真三轴模拟实验系统,系统开展了30 cm×30 cm×30 cm致密砂岩露头岩样水力压裂模拟实验。利用三维扫描仪量化评价压裂裂缝改造面积,结合岩体破裂时的实验泵压峰值,揭示裂缝起裂及延伸规律。结果量化了天然裂缝及弱面、应力差、压裂液体类型和施工排量条件下压裂裂缝起裂压力及其改造面积,揭示了地质工程因素对压裂裂缝延伸行为的影响规律,明确了地质工程参数对压裂裂缝延伸及复杂缝网形成的影响程度。结论天然裂缝及弱面发育、低应力差、低黏滑溜水和高排量是获得复杂裂缝形态的有利因素,为研究区块体积压裂工艺试验进行了实验佐证和理论依据。建议采用“大液量、大排量、扩大波及体积”为主的体积压裂思路及工艺技术,以获得复杂缝网的改造目标。

页岩气藏暂堵转向压裂裂缝扩展规律模拟

暂堵转向压裂技术是提高非常规油气产能的重要方法之一,在非常规油气藏储层改造中已经得到了广泛应用。为进一步认识威远地区页岩气藏暂堵转向压裂裂缝扩展特征,文中通过真三轴大物模实验研究了暂堵转向压裂压力响应与岩样破裂形态,验证了暂堵产生复杂缝网结构的可行性,明确了在不同应力差条件下的暂堵后裂缝起裂规律;同时采用ABAQUS软件黏结单元模拟裂缝的起裂与扩展,研究了不同水平应力差、不同偏转角下暂堵转向压裂裂缝扩展规律。研究结果表明:水平应力差对初次裂缝与暂堵转向裂缝的开启与扩展均具有显著的影响,水平应力差越小时,一次和二次压裂时岩样的破裂压力就越大,岩样越不容易被压碎。裂缝扩展阻力的不断增加,提升了缝内净压力与裂缝宽度;相交缝二次扩展后开启的新裂缝与一次压裂夹角越小,新裂缝扩展效果越好。该研究对威远地区现场压裂和缝网形态预测具有一定的理论指导和借鉴意义。

真三轴岩心暂堵转向压裂物理模拟实验

为进一步认识暂堵压裂裂缝扩展机制,采用新型制作方法浇筑水泥试件,使其带有预置的人工裂缝段和暂堵剂,通过真三轴大物模实验,研究了不同条件下暂堵压裂裂缝的扩展特征。研究表明:在支撑剂与10%质量比纤维混合铺置情况下,试件裂缝起裂方向与水平最大主应力方向一致,预制裂缝的存在导致起裂压力降低;当预制裂缝中充填4/16目颗粒型暂堵剂与支撑剂、纤维均匀混合或者充填20/80目颗粒型暂堵剂与支撑剂、纤维段塞铺置时,起裂压力明显升高,并且形成了复杂裂缝,说明裂缝的形成与暂堵剂和支撑剂的铺置方式密切相关;当预制裂缝中充填4/16目颗粒型暂堵剂与支撑剂、纤维段塞铺置时,压裂过程中有一定的增压效果,但生成裂缝未发生明显转向。该实验明确了不同暂堵转向压裂条件下裂缝扩展特征,为暂堵转向压裂工艺提供了实验技术支撑。

Case study on the mechanics of NPR anchor cable compensation for large deformation tunnel in soft rock in the Transverse Mountain area,China

A study was conducted to analyze the deformation mechanism of strongly weathered quartz schist in the Daliangshan Tunnel,located in the western Transverse Mountain area.A large deformation problem was experienced during the tunnel construction.To mitigate this problem,a support system was designed incorporating negative Poisson ratio(NPR)anchor cables with negative Poisson ratio effect.Physical model experiments,field experiments,and numerical simulation experiments were conducted to investigate the compensation mechanical behavior of NPR anchor cables.The large deformations of soft rocks in the Daliangshan Tunnel are caused by a high ground stress,a high degree of joint fracture development,and a high degree of surrounding rock fragmentation.A compensation mechanics support system combining long and short NPR anchor cables was suggested to provide sufficient counter-support force(approximately 350 kN)for the surrounding rock inside the tunnel.Comparing the NPR anchor cable support system with the original support system used in the Daliangshan tunnel showed that an NPR anchor cable support system,combining cables of 6.3 m and 10.3 m in length,effectively prevented convergence of surrounding rock deformation,and the integrated settlement convergence value remained below 300 mm.This study provides an effective scientific basis for resolving large deformation problems in deeply buried soft rocks in western transverse mountain areas.

Reversal of hyperglycemia in diabetic rats by portal vein transplantation of islet-like cells generated from bone marrow mesenchymal stem cells

AIM: To study the capacity of bone marrow mesenchymal stem cells (BM-MSCs) trans-differentiating into islet-like cells and to observe the effect of portal vein transplantation of islet-like cells in the treatment of streptozotocin-induced diabetic rat. METHODS: BM-MSCs were isolated from SD rats and induced to differentiate into islet-like cells under defined conditions. Differentiation was evaluated with electron microscopy, RT-PCR, immunofluorescence and flow cytometry. insulin release after glucose challenge was tested with ELiSA. Then allogeneic islet-like cells were transplanted into diabetic rats via portal vein. Blood glucose levels were monitored and islet hormones were detected in the liver and pancreas of the recipient by immunohistochemistry. RESULTS: BM-MSCs were spheroid adherent monolayers with high CD90, CD29 and very low CD45 expression. Typical islet-like cells clusters were formed after induction. Electron microscopy revealed that secretory granules were densely packed within the cytoplasm of the differentiated cells. The spheroid cells expressed islet related genes and hormones. The insulin-positive cells accounted for 19.8% and mean fluorescence intensity increased by 2.6 fold after induction. The cells secreted a small amount of insulin that was increased 1.5 fold after glucose challenge. After transplantation, islet-like cells could locate in the liver expressing islet hormones and lower the glucose levels of diabetic rats during d 6 to d 20.CONCLUSION: Rat BM-MSCs could be transdifferentiated into islet-like cells in vitro . Portal vein transplantation of islet-like cells could alleviate the hyperglycemia of diabetic rats.

Visceral hypersensitivity and altered colonic motility after subsidence of inflammation in a rat model of colitis

AIM:Irritable bowel syndrome(IBS)is a functional bowel disorder characterized by visceral hypersensitivity and altered bowel motility.There is increasing evidence suggesting the role of inflammation in the pathogenesis of IBS,which addresses the possibility that formerly established rat model of colitis could be used as an IBS model after the inflammation subsided. METHODS:Colitis was induced by intracolonic instillation of 4% acetic acid in male Sprague-Dawley rats.The extent of inflammation was assessed by histological examination and myeloperoxidase(MPO)activity assay.After subsidence of colitis,the rats were subjected to rectal distension and restraint stress,then the abdominal withdrawal reflex and the number of stress-induced fecal output were measured, respectively. RESULTS:At 2 days post-induction of colitis,the colon showed characteristic inflammatory changes in histology and 8-fold increase in MPO activity.At 7 days post-induction of colitis,the histological features and MPO activity returned to normal.The rats at 7 days post-induction of colitis showed hypersensitive response to rectal distension without an accompaning change in rectal compliance,and defecated more stools than control animals when under stress.CONCLUSION: These results concur largely with the characteristic features of IBS, visceral hypersensitivity and altered defecation pattern in the absence of detectable disease, suggesting that this animal model is a methodologically convenient and useful model for studying a subset of IBS.

Effect of Scutellariae Radix extract on experimental dextran-sulfate sodium-induced colitis in rats

AIM： To investigate the effect of Scutellariae Radix extract （SRE） on ulcerative colitis （UC） in rats induced by dextran-sulfate sodium （DSS）. METHODS： Colitis was induced in male Sprague-Dawley （SD） rats （170-180 g） by 4% dextran sulfate sodium （DSS, wt/v, MW 54000） in drinking water for 8 d. The treated rats received 4% DSS and SRE orally （100 mg/kg per day）. Control rats received either tap water or SRE only. Macroscopic assessment which included body weight changes, fecal occult blood and stool consistency were determined daily. At the appointed time, the rats were sacrificed and the entire colons were removed. The colon length and the myeloperoxidase （MPO） activity were measured. The severity of colitis was graded by morphological and histological assessments. The ion transport activity of the colonic mucosa was assessed by electrophysiological technique. RESULTS： Rats treated with oral administration of 4% DSS regularly developed clinical and macroscopic signs of colitis. Treatment with SRE relieved the symptoms, including the reduction in body weight, shortening 2nd ulceration of the colon. Administration of SRE also significantly reduced the histological damage induced by DSS. Moreover, the Isc responses of the colonic mucosa to forskolin, were suppressed after the induction of colitis. The stimulated ion transport activity of DSS-rats treated with SRE displayed significant improvement in the secretory responsiveness. CONCLUSION： SRE was effective in treating acute DSS- induced ulcerative colitis, as gauged by reduced clinical disease, improved macroscopic and histological damage scores, and enhanced recovery of normal colonic secretory function.

Evaluation of Surface Air Temperature Change over China and the Globe during the Twentieth Century in IAP AGCM4.0

Based on time series and linear trend analysis, the authors evaluated the performance of the fourth gen- eration atmospheric general circulation model developed at the Institute of Atmospheric Physics, Chinese Academy of Sciences （IAP AGCM4.0）, in simulating surface air temperature （SAT） during the twentieth century over China and the globe. The numerical experiment is con- ducted by driving the model with the observed sea surface temperature and sea ice. It is shown that IAP AGCM4.0 can simulate the warming trend of the global SAT, with the major wanning regions in the high latitudes of the Northern Hemisphere and the mid-latitudes of the South- ern Hemisphere. While the simulated trend over the whole globe is close to the observation, the model trader- estimates the observed trend over the continents. More- over, the model simulates the spatial distribution of SAT in China, with a bias of approximately -2℃ in eastern China, but with a more serious bias in western China. Compared with the global mean, however, the correlation coefficient between the simulation and observation in China is significantly lower, indicating that there is large uncertainty in simulating regional climate change.

Statistically Downscaled Summer Rainfall over the Middle-Lower Reaches of the Yangtze River

The summer rainfall over the middle-lower reaches of the Yangtze River valley (YRSR) has been estimated with a multi-linear regression model using principal atmospheric modes derived from a 500 hPa geopotential height and a 700 hPa zonal vapor flux over the domain of East Asia and the West Pacific.The model was developed using data from 1958 92 and validated with an independent prediction from 1993 2008.The independent prediction was efficient in predicting the YRSR with a correlation coefficient of 0.72 and a relative root mean square error of 18%.The downscaling model was applied to two general circulation models (GCMs) of Flexible Global Ocean-Atmosphere-Land System Model (FGOALS) and Geophysical Fluid Dynamics Laboratory coupled climate model version 2.1 (GFDL-CM2.1) to project rainfall for present and future climate under B1 and A1B emission scenarios.The downscaled results pro-vided a closer representation of the observation compared to the raw models in the present climate.In addition,compared to the inconsistent prediction directly from dif-ferent GCMs,the downscaled results provided a consistent projection for this half-century,which indicated a clear increase in the YRSR.Under the B1 emission scenario,the rainfall could increase by an average of 11.9% until 2011 25 and 17.2% until 2036 50 from the current state;under the A1B emission scenario,rainfall could increase by an average of 15.5% until 2011 25 and 25.3% until 2036 50 from the current state.Moreover,the increased rate was faster in the following decade (2011 25) than the latter of this half-century (2036 50) under both emissions.

Induction of experimental acute ulcerative colitis in rats by administration of dextran sulfate sodium at low concentration followed by intracolonic administration of 30% ethanol

Several models of experimental ulcerative colitis have been reported previously. However, none of these models showed the optimum characteristics. Although dextran sulfate sodium-induced colitis results in inflammation resembling ulcera-tive colitis, an obvious obstacle is that dextran sulfate sodium is very expensive. The aim of this study was to develop an inex-pensive model of colitis in rats. Sprague-Dawley rats were treated with 2% dextran sulfate sodium in drinking water for 3 d fol-lowed by an intracolonic administration of 30% ethanol. The administration of 2% dextran sulfate sodium followed by 30% ethanol induced significant weight loss, diarrhea and hematochezia in rats. Severe ulceration and inflammation of the distal part of rat colon were developed rapidly. Histological examination showed increased infiltration of polymorphonuclear leukocytes, lymphocytes and existence of cryptic abscesses and dysplasia. The model induced by dextran sulfate sodium at lower concentra-tion followed by 30% ethanol is characterized by a clinical course, localization of the lesions and histopathological features similar to human ulcerative colitis and fulfills the criteria set out at the beginning of this study.

Gastric cancer induced by N-methyl-N'-nitro-N-nitrosoguanidine in rat with ulcers and expressions of ras and c-erbB2 genes

Objective: To observe the series of pathological changes during the development of gastric adenocarcinoma in ulcerative rats induced by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), and the expression profile of related oncogenic protein.Methods: MNNG was administered in rats with ulcers due to acetic acid treatment to induce gastric cancer, and the protein expressions of ras and c-erbB2 genes in the ulcer were examined immunohistochemically along with pathological examination.Results: The incidence of gastric adenocarcinoma in the model group reaches 40% (6/15), while none of the rats developed cancer in the control group with ulcers.Positive expressions of the proteins of p21ras and c-erbB2 were observed in the tissues undergoing canceration in the 6 rats of model group, but were not observed in the 5 control rats; p53 protein expression, however, failed to be detected in both groups.Conclusion: A new animal model of gastric cancer has been established in rats with gastric ulcer after MNNG treatment, which may facilitate the pharmacological research of gastric cancer.

East China Summer Rainfall during ENSO Decaying Years Simulated by a Regional Climate Model

The performance of the Climate version of the Regional Eta-coordinate Model （CREM）, a regional climate model developed by State Key Laboratory of Numerical modeling for Atmospheric Science and Geophysical Fluid Dynamics/Institute of Atmospheric Physics （LASG/IAP）, in simulating rainfall anomalies during the ENSO decaying summers from 1982 to 2002 was evalu- ated. The added value of rainfall simulation relative to reanalysis data and the sources of model bias were studied. Results showed that the model simulated rainfall anomalies moderately well. The model did well at capturing the above-normal rainfall along the Yangtze River valley （YRV） during E1 Nifio decaying summers and the below and above-normal rainfall centers along the YRV and the Huaihe River valley （HRV）, respectively, during La Nifia decaying summers. These features were not evident in rainfall products derived from the reanalysis, indicating that rainfall simulation did add value. The main limitations of the model were that the simulated rainfall anomalies along the YRV were far stronger and weaker in magnitude than the observations during E1 Nifio decaying summers and La Nifia decaying summers, respectively. The stronger magnitude above-normal rainfall during E1 Nifio decaying summers was due to a stronger northward transport of water vapor in the lower troposphere, mostly from moisture advection. An artificial, above-normal rainfall center was seen in the region north to 35°N, which was associated with stronger northward water vapor transport. Both lower tropospheric circulation bias and a wetter model atmosphere contributed to the bias caused by water vapor transport. There was a stronger southward water vapor transport from the southern boundary of the model during La Nifia decaying summers; less remaining water vapor caused anomalously weaker rainfall in the model as compared to observations.

Preventive and therapeutic effects of NF-kappaB inhibitor curcumin in rats colitis induced by trinitrobenzene sulfonic: acid

AIM: To ascertain the molecule mechanism of nuclear factor-KB (NF-κB) inhibitor curcumin preventive and therapeutic effects in rats' colitis induced by trinitrobenzene sulfonic acid (TNBS). METHODS: Sixty rats with TNBS-induced colitis were treated with 2.0% curcumin in the diet. Thirty positive control rats were treated with 0.5% sulfasalazine (SASP). Thirty negative control rats and thirty model rats were treated with general diet. Changes of body weight together with histological scores were evaluated. Survival rates were also evaluated. Cell nuclear NF-κB activity in colonic mucosa was evaluated by using electrophoretic mobility shift assay. Cytoplasmic IκB protein in colonic mucosa was detected by using Western Blot analysis. Cytokine messenger expression in colonic tissue was assessed by using semiquantitative reverse-transcription polymerase chain reaction. RESULTS: Treatment with curcumin could prevent and treat both wasting and histopathologic signs of rats with TNBS-induced intestinal inflammation. In accordance with these findings, NF-κB activation in colonic mucosa was suppressed in the curcumin-treated groups. Degradations of cytoplasmic IκB protein in colonic mucosa were blocked by curcumin treatment. Proinflammatory cytokine messenger RNA expression in colonic mucosa was also suppressed. CONCLUSION: This study shows that NF-κB inhibitor curcumin could prevent and improve experimental colitis in murine model with inflammatory bowel disease (IBD). The findings suggest that NF-κB inhibitor curcumin could be a potential target for the patients with IBD.

A P.hysical Numerical Ionospheric Model and Its Simulation Results

This paper describes the construction of a one-dimensional time-dependent theoretical ionospheric model, which is based on numerical solution of continuity and momentum equations for , and NO+. The model is designed to have an option to incorporate the observational ionospheric characteristic parameters into the numerical model to indirectly determine the upper boundary condition when solving the transport equations of O+. A preliminary simulation result of the model when used to simulate the ionosphere during April 18 ~ May 10, 1998, which includes both quiet and disturbed periods, showed that the model constructed is able to reproduce the observational results reasonably well both for quiet and disturbed periods.

NO3- and HSO3- Induced Corrosion of Simulated Archaeological Iron in Humid-Acid Atmosphere

NO3- and HSO3- induced corrosion of simulated archaeological iron in humid-acid atmosphere was studied by using periodic wet-dry tests. Surface tension tests, electrochemical impedance measurement, stereoscopic microscopy and energy dispersive atomic X-ray analysis (EDAX) were used to investigate the corrosion processes and products. The results of mass loss measurement showed that the corrosion kinetics can be approximately described by △W - AtB. With the addition of NO3-, the value of B increases. NO3- acts as an inhibitor in the HSO3- bearing pollutant during the corrosion process. Both the electrochemical impedance measurement and surface tension test confirmed the result of mass loss measurement. In addition, we found that the attack of anions to the metal at the initial corrosion stage was in good agreement with their surface activity.

Does knowledge of physical activity recommendations increase physical activity among Chinese college students？ Empirical investigations based on the transtheoretical model

Background: Based on the transtheoretical model, the current study investigated whether awareness of physical activity(PA) recommendations had an impact on the stages of PA behavior change and levels of PA among Chinese college students.Methods: In Study 1, with a cross-sectional study design, 9826 students were recruited, and their knowledge of international PA recommendations,PA stage distribution, and self-reported PA level were surveyed. Pearson's χ2 test was used to test whether those participants who were aware and not aware of PA guidelines were equally distributed across the stages of PA behavior, and independent t test was conducted to test the group difference in the actual levels of PA. In Study 2, 279 students who were not aware of the PA recommendations were randomly allocated to either an intervention group or a control group, and only those in the intervention group were presented with international PA guidelines. In both groups,students' PA stages and PA level were examined before the test and then 4 months post-test. Mc Nemar's test for correlated proportions and repeated-measures analysis of variance were conducted to examine the changes in PA stage membership and PA level after the intervention.Results: Study 1 results revealed that only 4.4% of the surveyed students had correct knowledge of PA recommendations. Those who were aware of the recommendations were in later stages of PA behavior(χ~2(4) = 167.19, p < 0.001). They were also significantly more physically active than those who were not aware of the recommendations(t(443.71) = 9.00, p < 0.001, Cohen's d = 0.53). Study 2 results demonstrated that the intervention group participants who were at the precontemplation and contemplation stages at the pre-test each progressed further in the PA stages in the post-test(χ~2(1) = 112.06, p < 0.001; χ~2(1) = 118.76, p = 0.03, respectively), although no significant change in PA level was observed(t(139) < 1, p = 0.89).Conclusion: The results showed that awareness of the PA recommendations was associated with higher stages and levels of PA behavior, and a brief educational exposure to PA recommendations led to improved stages of PA behavior but no change in the levels of PA among Chinese college students. More effective public health campaign strategies are needed to promote the dissemination of the PA recommendations and to raise the awareness of the Chinese student population.

The pain-related behavior changes correlate with the bone damage in a rat model of rheumatoid arthritis

In view of the extensive bone damage in rheumatoid arthritis, we used a commonly utilized animal model to detect behavioral changes in pain-related and the bone damage during the early disease, and to explore the correlation between bone damage and pain-related behavioral changes. Methods： Arthritis were induced in Sprague-Dawley （SD） male rats by injecting complete Freund＇s adjuvant （CFA） into the tails. Pain-related behavior changes were studied using the Hargreaves, VonFrey, and acetone tests on the 0, 7, 14, day and 28 day after CFA injection. The rats were sacrificed according the same schedule. The bone damage of the right proximal tibia was studied by microCT scan and bone histological slices. Results： Animals developed soft tissue inflammation and polyarthritis on 7 days after CFA injection, and arthritic score proved obvious arthritis were established within the study period. Mechanical hyperalgesia and cold allodynia were present in the affected hind paw from the 7 day through the 28 day, but the heat hyperalgesia and the mechanical allodynia lasted a short time after CFA injection. Trabecular bone number （Tb.N）, Tissue Mineral Content （TMC） and Bone Volume to Tissue Volume （BV/TV） in the proximal tibia by microCT scan were also reduced after induction, especial 14 days after CFA injection. The bone histologicalslices showed the trabecular bone and proteoglycan diminished, the bone damage severity scores became more severely on the 7 day after CFA injection. Using analysis of covariance, these changes had statistical significance compared with baseline. By linear regression analysis demonstrated mechanical hyperalgesia and cold allodynia correlated well with arthritic score, bone damage parameters and bone damage severity scores. Conclusion： Adjuvant-induced arthritis （AA） were observed after CFA injection and lasted within the later experimental period. Pain-related behavioral changes were observed in the early time of AA. Bone damage was also occurred with arthritis development. Pain-related behavioral change correlated well with arthritic score and bone damage parameters

Prunella vulgaris L. extract improves cellular immunity in MDR-TB challenged rats

Objective： To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis （MDR-TB）. Methods： Experimental animal model in rats was induced by MDR-TB. Normal group model group and Prunella vulgaris L. group were set up. The contents of IFN-7, IL-4, IL-10 and IL-12 were examined by ELISA. Their genome mRNAs were extracted, the target genes were amplified by PCR. RT-PCR was used to detect the mRNA levels of them. Results： The content of IFN-q, of the extract of Prunella vulgaris L. group was 1.98±0.67 pg/ml, IL-4 was 6.47±1.46 pg/ml, IL-10 was 12.13±3.43 pg/ml and IL-12 was 3.02±0.86 pg/ml. Compared with the model group, Prunella vulgaris L. group was notable difference in serum IFN-γ, IL-12 and IL-10 （P〈0.05）. The mRNA levels of IFN-γ, IL-12 increased and IL-10 decreased obviously, the differences were quite significant （P〈0.05）, but IL-4 had no obvious change. Conclusion： The extract of Prunella vulgaris L. can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription, accordingly provide the theory basis of healing of tuberculosis with it.

Effect of Chloride on the Atmospheric Corrosion of Cast Iron in Sulphur or Nitrogen-Bearing Pollutant Environment

The effect of chloride on the atmospheric corrosion of cast iron in sulphur or nitrogen-bearing pollutant was investigated by using periodic wet-dry test, electrochemical experiment and surface tension test. Scanning electron microscopy coupled with energy dispersive atomic （EDAX） and stereoscopic microscopy was used to identify the corrosion processes and products. Cl^- and NO3^- were shown accelerating effects during the whole corrosion process but depression effects were observed in Cl^- and HSO3^- bearing pollutant at the initial corrosion stage. However, with the corrosion going on, the depression effects was less obviously and the initial corrosion process was investigated from the viewpoint of surface activity. At the initial corrosion stage, the corrosion rate was proportional to the adsorptivity of anions, but as corrosion went on, the penetration effect of anions and different characteristics of the corrosion products began to dominate the corrosion process, which led to changes on the corrosion rate.

HELICITY ANALYSIS FOR HIGH-WIND AND UNCOMMON RAINSTORM PROCESSES OVER YUNNAN IN EARLY SUMMER

Helicity was applied to analyze several high-wind and rainstorm processes, which occurred from May to June, 2001 over Yunnan in early summer. The results of diagnostic analyses show that the rainstorm occurs in the area in which hp is positive at 700 hPa and energy is unstable. The change of helicity can reflect the movement and development of synoptic system and the position and intensity of the rainstorm. The value of hp is a negative center at the upper level and a positive at the lower level over the rainstorm position; moreover it can reflect the characteristics of vertical distribution and rotational motion.