The authors’ surveys in May-June 1999 (two cruises) at six sampling stations near nuclear power plants (NPP) and marine fish culture zones in Daya Bay, Guangdong, revealed species composition, densities and body-size...The authors’ surveys in May-June 1999 (two cruises) at six sampling stations near nuclear power plants (NPP) and marine fish culture zones in Daya Bay, Guangdong, revealed species composition, densities and body-size of the sea surface microlayer (SM) zooplankton (>35 μm). Results showed that protozoans and copepod nauplii were the predominant components, accounting for 65.40% to 95.56% of total zooplankton in abundance. The size-frequency distributions showed that the frequency of micro-zooplankton (0.02-0.2 mm) reached 0.8235. The SM zooplankton community structure revealed in the present study was quite different from that revealed by investigations in the 1980s in Daya Bay. Difference of sampling method has important influence on the obtained zooplankton community structure. SM zooplankton consisted of micro- and mesozooplankton (0.2-2.0 mm), with micro-zooplankton being predominant. Some possible cause-effect relations between the zooplankton community structure and mariculture, nuclear power plants cooling systems and sampling method are discussed.展开更多
Purpose: Physical activity (PA) and sedentary behavior (SB) are increasingly considered independent health behaviors. Additionally, current research suggests that both controlled and automatic determinants accoun...Purpose: Physical activity (PA) and sedentary behavior (SB) are increasingly considered independent health behaviors. Additionally, current research suggests that both controlled and automatic determinants account for their adoption. The purpose of this article was to identify intention-automaticity profiles toward PA and screen-based SB and to examine how those profiles are associated with different behavioral patterns. Method: Two cross-sectional studies based on self-report questionnaires were conducted with French high school students (Study 1: n = 198; Study 2: n = 185). Results: In all, 4 distinct motivational profiles appeared. The first 3 clusters emerged in both studies: "PA" (high levels of automaticity and intention for PA, low levels of automaticity and intention for screen-based SB); "screen" (high levels of automaticity and intention for screen-based SB, low levels of automatieity and intention for PA), and "mixed" (high levels of all variables), whereas the fourth cluster was observed only in Study 2: "high control" (below-mean levels of automaticity, high levels of intention toward both PA and screen-based SB). Adolescents with a screen profile displayed the least healthy behavioral pattern, whereas those in the PA profile demonstrated the most favorable behaviors. Conclusion: Future research is needed to extend these results to other populations using complementary assessment methods of automatic psychological processes and PA and SB behaviors.2018 Published by Elsevier B.V.on behalf of Shanghai University of Sport. This is an open access article under the CC BY-NC-ND license.(http://creativecommons.org/licenses/by-nc-nd/4.0/).展开更多
The uncertainty in assessing the numerous atmospheric pollutants transported via wind from arid and semi-arid regions is affecting the glacial ecosystem. In our study area due to the complexity of the system, a promin...The uncertainty in assessing the numerous atmospheric pollutants transported via wind from arid and semi-arid regions is affecting the glacial ecosystem. In our study area due to the complexity of the system, a prominent seasonal difference noticed among major ions(Ca^(2+), Mg^(2+), SO_4^(2-), and NO_3^-). There is a need for understanding the ions cycling as a whole and the directionality of the feedback loops in the system. Therefore, we provide an appraisal of our current hypothesis for seasonal difference in major ion concentration from snow samples for two corresponding years(2013 and 2015) at Dokriani Glacier. A systematic study of chemical compositionsin the shallow snow pit from Dokriani Glacier was undertaken for the pre-monsoon season to understand the cycling of major ions from atmosphere to solute acquisition process. The intimating connections of ions cycling in snow and its temporal behavior was observed and analyzed through various statistical tests. Among major ions, the SO_4^(2-)has the highest concentration among anions on an average considered as 14.21% in 2013 and 29.46% in 2015. On the other side Ca^(2+) is the dominant cation contributing 28.22% in 2013 and 15.3% in 2015 on average. The average ratio of Na+/Cl-was higher in 2013 whereas lower in 2015. The backward trajectory analysis suggests the possible sources of the ions transported from Central Asia through the Western Disturbance(WD) as a prominent source of winter precipitation mainly in the Central Himalaya. Ionicconcentration of Ca^(2+) in cations was highly dominated while in anion SO_4^(2-)played the major role. Factor analysis and correlation matrix suggested that, the precipitation chemistry is mostly influenced by anthropogenic, crustal, and sea salt sources over the studied region. The elemental cycling through ocean, atmosphere and biosphere opens up new ways to understand the geochemical processes operating at the glacierized catchments of the Himalaya. Moreover, increasing the field-based studies in the coming decades would also have the certain advantage in overcoming the conceptual and computational geochemical modelling difficulties.展开更多
Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production ...Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production and the lowest production of some hazardous by-products, especially acetic acid, were obtained in the media containing 0.085 mol·L-1 glucose and 0.019 mol·L-1 nitrogen (carbon-nitrogen ratio, 4.47:1). After thermal induction, when the concentrations of glucose and nitrogen in the media were 0.065 mol·L-1 and 0.017 mol·L-1 , respectively (carbon-nitrogen ratio, 3.82:1), the productivity of human-like collagen per cell was the highest while that of acetic acid was the lowest. The extended analysis showed that the production of lactic acid and propionic acid increased while that of some intermediate acids of the tricarboxylic acid cycle decreased if the dose of glucose increased.展开更多
AIM: To investigate the relationship between donor liver cold preservation, lung surfactant (LS) changes and acute lung injury (ALI) after liver transplantation. METHODS: Liver transplantation models were estab-...AIM: To investigate the relationship between donor liver cold preservation, lung surfactant (LS) changes and acute lung injury (ALI) after liver transplantation. METHODS: Liver transplantation models were estab- lished using male Wistar rats. Donor livers were pre- served in University of Wisconsin solution at 4 ℃ for different lengths of time. The effect of ammonium pyr- rolidinedithiocarbamate (PDTC) on ALI was also detect- ed. All samples were harvested after 3 h reperfusion.The severity of AU was evaluated by lung weight/body weight ratio, lung histopathological score, serum nitric oxide (NO) and endothelin (ET)-I levels, lung tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels. Lung surfactants (LSs) were determined by micellar electrokinetic capillary chromatography. RESULTS: With extended donor liver cold preservation time (CPT), lung histopathological scores, serum ET-i levels, lung weight/body weight ratio and the level of TNF-α and IL-1β in lung were increased significantly in the 180-min group compared with the sham group (3.16± 0.28 vs 1.12 ± 0.21, P 〈 0.001; 343.59±53.97 vs 141.53± 48.48, P 〈 0.001; 0.00687 ±0.00037 vs 0.00557 ±0.00056, P 〈 0.001; 17.5 ± 3.0 vs 1,3 ± 0.3, P 〈 0.001; 10.8± 2.3 vs 1.8 ± 0.4, P 〈 0.001), but serum NO levels decreased remarkably (74.67 ± 10.01 vs 24.97 ± 3.18, P 〈 0.001). The expression of lung phos- phatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) and phosphatidylserine (PS) increased when CPT was 〈 120 rain, and decreased when CPT was 〉 180 min (PC: 1318.89 ± 54.79 vs 1011.18± 59.99, P 〈 0.001; PE: 1504.45± 119.96 vs 1340.80±76.39, P = 0.0019; PI: 201.23 ± 34.82 vs 185.88 ± 17.04, P = 0.2265; PS: 300.43±32.95 vs 286.55± 55.55, P = 0.5054). All these ALI-associated indexes could be partially reversed by PDTC treatment.展开更多
To enlarge the detection range of traditional fiber-optic spectrometer,two rotating parabolic mirrors were first used in the design of fiber-optic collector for spectrometer,which can extend the detection range of spe...To enlarge the detection range of traditional fiber-optic spectrometer,two rotating parabolic mirrors were first used in the design of fiber-optic collector for spectrometer,which can extend the detection range of spectrometer from centimeter-scale to50m,as a super-low altitude remote sensing detector under passive lighting conditions.According to the performance requirements of fiber-spectrometer for optical collector,this study deduced the calculation formulae of design size of parabolic optical collector applied to different sensitivity spectrometers,different detection targets,different detection ranges and different weathers.Based on the calculation results,the model of fiber-optic collector with a diameter of16mm and a height of7.8mm was designed by ZEMAX.And then,TracePro,a light simulation software,was applied to ray tracing.The simulation results of optical radiation magnification agrees well with theoretical value,and the relative error is less than0.78%.This optical collector can effectively extend the detection range of spectrometer and expand its application fields and scopes.Compared with traditional refractive optical collector,it has several advantages of compact structure,low weight and low cost.展开更多
A coupled system of the interdecadal sea-air oscillator model is studied. The E1 Nifio-southem oscillation (ENSO) atmospheric physics oscillation is an abnormal phenomenon involved in the tropical Pacific ocean-atmo...A coupled system of the interdecadal sea-air oscillator model is studied. The E1 Nifio-southem oscillation (ENSO) atmospheric physics oscillation is an abnormal phenomenon involved in the tropical Pacific ocean-atmosphere interactions. The oscillator model is involved with the variations of both the eastern and western Pacific anomaly pat- terns. This paper proposes an ENSO atmospheric physics model using a method of the perturbation theory. The aim is to create an asymptotic solving method for the ENSO model. Employing the perturbed method, the asymptotic solution of corresponding problem is obtained, and the asymptotic behaviour of the solution is studied. Thus we can obtain the prognoses of the sea surface temperature anomaly and related physical quantities.展开更多
The flesh of spaghetti squash separates into strands when cooked. The purpose of this paper is to investigate the cause of strand separation (during cooking) by soaking for 24 h at 35 ℃ in solutions with three kind...The flesh of spaghetti squash separates into strands when cooked. The purpose of this paper is to investigate the cause of strand separation (during cooking) by soaking for 24 h at 35 ℃ in solutions with three kinds of pectin extractant. The changes in strand separation, firmness, histological structure and the pectin of flesh during soaking in 0.01 N HCI solution (pH 2.0), 0.035 M ammonium oxalate solution (pH 4.0) or 2% sodium hexametaphosphate solution (pH 4.0) were investigated. When flesh was soaked in the HCI solution, the separation into strands and removal of calcium and magnesium were greater than that soaked in other pectin extractants. High methoxyl pectin was extracted by soaking in HC1 solution (pH 2.0) due to removal of polyvalent cations. This result shows that high methoxyl pectin glues strands together in the flesh of spaghetti squash. The shape of the cells which constituted strands was round; on the other hand, that of cells surrounded strands was elongated. When cooked in boiling water or soaked at pH 2.0, the shape of the former cells was maintained, but the latter cells, which contributed to adhesion between strands, broke down. Thus, the flesh separated into strands. When flesh was boiled for 15-30 min, pectin degraded and dissolved in the cooking solution; consequently, the flesh separated into strands and also the middle lamella of cell walls of strands separated. However, pectin remaining in strands maintained their crispness.展开更多
The purpose of this paper is to investigate the relationship between pectic substances and the separation into strands during cooking of spaghetti squash. Spaghetti squash flesh separated into strands when boiled. Pec...The purpose of this paper is to investigate the relationship between pectic substances and the separation into strands during cooking of spaghetti squash. Spaghetti squash flesh separated into strands when boiled. Pectic substances of raw and cooked flesh were fractionated into three reagents. The galacturonic acid compositions of hydrochloric acid (HC1)-soluble pectin (PA), sodium acetate buffer-soluble pectin (PB) and sodium hexametaphosphate-soluble pectin (PC) of raw flesh were 69.0%, 28.9% and 2.1%, respectively. Also, the degree of esterification (DE) ofPA, PB and PC was 67.4%, 61.5% and 55.6%, respectively. The DE of pectin was from the greatest to the least: PA 〉 PB 〉 PC, respectively, and the diethylaminoethyl (DEAE)-cellulose column chromatograms of PA and PB showed that they were comparatively in high methoxyl pectin. Therefore, about 50% of pectic substances in flesh were released into a cooking solution during 15-30 min of cooking. Highmethoxylpectin was degraded by β-elimination during boiling. Consequently, the flesh was separated into strands. This suggests that highmethoxylpectin glues strands together in the flesh of spaghetti squash.展开更多
Studying the ecology of ants can be a powerful tool for conservation, While the effect of logging is mainly investigated by the comparison of species richness and composition, the impact on individual species are ofte...Studying the ecology of ants can be a powerful tool for conservation, While the effect of logging is mainly investigated by the comparison of species richness and composition, the impact on individual species are often neglected. This study investigated the effect of selective logging on the nest density, foraging range and colony size on the ground-dwelling ant Aphaenogaster swammerdami in Kirindy forest--Madagascar. This ant is a common ground-dwelling species in Kirindy, a western dry deciduous forest of Madagascar. Sampling was done in two sites of the forest: One part that was selectively lodged and another that have not been logged. Here we show that selective logging led to a decrease in colony size and density, while the foraging range seemed to be unaffected. Higher desiccation stress and lower food availability in the logged forest are most likely to be responsible for these results.展开更多
The future climate dynamical downscaling method is that output of general circulation models( GCMs) is employed to provide initial conditions,lateral boundary conditions,sea surface temperatures,and initial land surfa...The future climate dynamical downscaling method is that output of general circulation models( GCMs) is employed to provide initial conditions,lateral boundary conditions,sea surface temperatures,and initial land surface conditions to regional climate models( RCMs). There are two methods of downscaling: offline coupling and online coupling. The two kinds of coupling methods are described in detail by coupling the Weather Research and Forecasting model( WRF) with the Institute of Atmospheric Physics of Chinese Academy of Sciences Atmospheric General Circulation Model Version 4. 0( IAP AGCM4. 0) in the study. And the extreme precipitation event over Beijing on July 212012 is simulated by using the two coupling methods. Results show that online coupling method is of great value in improving the model simulation. Furthermore,the data exchange frequency of online coupling has some effect on simulation result.展开更多
This research was carried out to study the effect of fermentation on the chemical composition, anti-nutrient content, PH, titratable acidity, and microbiological changes of millet and soyabean blend. Millet and soyabe...This research was carried out to study the effect of fermentation on the chemical composition, anti-nutrient content, PH, titratable acidity, and microbiological changes of millet and soyabean blend. Millet and soyabean composite flours were mixed in gram of six combinations as follows millet and soyabean (A) = 100:0, millet and soyabean (B) = 90:10, millet and soyabean (C) = 80:20, millet and soyabean (D) = 70:30, millet and soyabean (E) = 60:40, millet and soyabean (F) = 50:50 and subjected to natural fermentation for 72 h. The following bacteria isolates were obtained from the fermentation; Lacobacillus fermentum, L. acidophilus, L. bulgaricus, L. plantarum, L. dextranicum, L. rhamnosus, L. delbrueckii, L. leichemanii, L. divergens, L. reuteri, L. jenseni, L. casei, L. salivarius, L. cellubiosus, Leuconostoc mesenteroide and Pediococcus acidilactis, of which L. plantarum was the most dominant the throughout the period of fermentation. There was decrease in pH with increase in TTA in all the samples. The result of the proximate analysis revealed a marginal increase in crude protein content of each sample (from 44.41 to 63.14, from 11.02 to 24.02, from 16.64 to 23.10, from 20.83 to 26.93, from 25.43 to 30.12, 39.12 to 35.86 and from 40.66 to 54.24%) There was increase in ash content and decrease in carbohydrate, fibre and fat contents of the fermented samples. Results from this research also show significant reduction in anti-nutritional content which are hydrogen cyanide, oxalate and phytate. Fermentation has modified the microbial and nutritional quality of the millet and soyabean blend and this has greatly improved the nutrient content of the blend.展开更多
Corrosion behavior of AZ91 magnesium alloy under NaCl particle deposition condition was investigated by gravimetric method and surface analysis technique.It was found that the mass gain increased rapidly at the beginn...Corrosion behavior of AZ91 magnesium alloy under NaCl particle deposition condition was investigated by gravimetric method and surface analysis technique.It was found that the mass gain increased rapidly at the beginning of exposure and then slowly with time.The corrosion morphologies were observed and the results showed that NaCl deposition resulted in the occurrence of localized corrosion.The composition of corrosion product was analyzed using X-ray photo electron spectroscopy.It was suggested that the corrosion product was a mixture of oxide and hydroxide of magnesium and aluminum.展开更多
The effect of Batroxobin on spatial memory disorder of left temporal ischemic rats and the expression of HSP32 and HSP70 were investigated with Morri`s water maze and immunohistochemistry methods. The results show... The effect of Batroxobin on spatial memory disorder of left temporal ischemic rats and the expression of HSP32 and HSP70 were investigated with Morri`s water maze and immunohistochemistry methods. The results showed that the mean reaction time and distance of temporal ischemic rats in searching a goal were significantly longer than those of the sham-operated rats and at the same time HSP32 and HSP70 expression of left temporal ischemic region in rats was significantly increased as compared with the sham-operated rats. However, the mean reaction time and distance of the Batroxobin-treated rats were shorter and they used normal strategies more often and earlier than those of ischemic rats. The number of HSP32 and HSP70 immune reactive cells of Batroxobin-treated rats was also less than that of the ischemic group. In conclusion, Batroxobin can improve spatial memory disorder of temporal ischemic rats; and the down-regulation of the expression of HSP32 and HSP70 is probably related to the attenuation of ischemic injury.展开更多
Purpose: To evaluate the effect of a hydroethanolic extract of Moltkia coerulea ointment (MCO) on the healing of excision wound in a rat model. Methods: Circular surgical full thickness excision wound, with 314 mm...Purpose: To evaluate the effect of a hydroethanolic extract of Moltkia coerulea ointment (MCO) on the healing of excision wound in a rat model. Methods: Circular surgical full thickness excision wound, with 314 mm2 size, was induced in the anterior-dorsal side of each rat. Three different doses of MCO (1%, 3% and 6%) were administrated. On Day 3, 7, 14 and 21, the tissue was sampled and immune cells, fibroblasts and fibrocytes distribution per one mm2 of wound area, collagen density and re-epithelialization were analyzed. Moreover, the total flavnoid, phenols and anti-oxidant potential of the MCO were evaluated. Ultimately, the percentage of wound contraction in different groups was compared with each other. Results: Hydroethanolic extract of MCO significantly (p 〈 0.05) increased wound contraction percentage. The animals in medium and high dose MCO-treated groups exhibited remarkably (p 〈 0.05) higher fibroblast and fibrocyte distribution and signifcantly (p 〈 0.05) lower immune cells infiltration. On Day 7 after injury, MCO up-regulated neovascularization in a dose-dependent way. Conclusion: Our data showed that MCO shortened the inflammation phase by provoking the fibroblast proliferation. Moreover, MCO promoted the healing process by up-regulating the angiogenesis and provoking the structural cells proliferation as well as increasing the collagen synthesis, cross-linking, and deposition.展开更多
The paper studies the ground vertical deformation and the geoid undulation caused by loading of neighboring buildings, based on the loading tides theory. The influence on elevation is also considered. The results show...The paper studies the ground vertical deformation and the geoid undulation caused by loading of neighboring buildings, based on the loading tides theory. The influence on elevation is also considered. The results show that the ground vertical deformation and the geoid undulation both reach millimeter magnitude. Therefore, it is obvious that the building loading significantly affects the precise engineering surveying, and it must be seriously considered in application.展开更多
文摘The authors’ surveys in May-June 1999 (two cruises) at six sampling stations near nuclear power plants (NPP) and marine fish culture zones in Daya Bay, Guangdong, revealed species composition, densities and body-size of the sea surface microlayer (SM) zooplankton (>35 μm). Results showed that protozoans and copepod nauplii were the predominant components, accounting for 65.40% to 95.56% of total zooplankton in abundance. The size-frequency distributions showed that the frequency of micro-zooplankton (0.02-0.2 mm) reached 0.8235. The SM zooplankton community structure revealed in the present study was quite different from that revealed by investigations in the 1980s in Daya Bay. Difference of sampling method has important influence on the obtained zooplankton community structure. SM zooplankton consisted of micro- and mesozooplankton (0.2-2.0 mm), with micro-zooplankton being predominant. Some possible cause-effect relations between the zooplankton community structure and mariculture, nuclear power plants cooling systems and sampling method are discussed.
文摘Purpose: Physical activity (PA) and sedentary behavior (SB) are increasingly considered independent health behaviors. Additionally, current research suggests that both controlled and automatic determinants account for their adoption. The purpose of this article was to identify intention-automaticity profiles toward PA and screen-based SB and to examine how those profiles are associated with different behavioral patterns. Method: Two cross-sectional studies based on self-report questionnaires were conducted with French high school students (Study 1: n = 198; Study 2: n = 185). Results: In all, 4 distinct motivational profiles appeared. The first 3 clusters emerged in both studies: "PA" (high levels of automaticity and intention for PA, low levels of automaticity and intention for screen-based SB); "screen" (high levels of automaticity and intention for screen-based SB, low levels of automatieity and intention for PA), and "mixed" (high levels of all variables), whereas the fourth cluster was observed only in Study 2: "high control" (below-mean levels of automaticity, high levels of intention toward both PA and screen-based SB). Adolescents with a screen profile displayed the least healthy behavioral pattern, whereas those in the PA profile demonstrated the most favorable behaviors. Conclusion: Future research is needed to extend these results to other populations using complementary assessment methods of automatic psychological processes and PA and SB behaviors.2018 Published by Elsevier B.V.on behalf of Shanghai University of Sport. This is an open access article under the CC BY-NC-ND license.(http://creativecommons.org/licenses/by-nc-nd/4.0/).
基金funded by the Department of Science and Technology,Government of India,New Delhi
文摘The uncertainty in assessing the numerous atmospheric pollutants transported via wind from arid and semi-arid regions is affecting the glacial ecosystem. In our study area due to the complexity of the system, a prominent seasonal difference noticed among major ions(Ca^(2+), Mg^(2+), SO_4^(2-), and NO_3^-). There is a need for understanding the ions cycling as a whole and the directionality of the feedback loops in the system. Therefore, we provide an appraisal of our current hypothesis for seasonal difference in major ion concentration from snow samples for two corresponding years(2013 and 2015) at Dokriani Glacier. A systematic study of chemical compositionsin the shallow snow pit from Dokriani Glacier was undertaken for the pre-monsoon season to understand the cycling of major ions from atmosphere to solute acquisition process. The intimating connections of ions cycling in snow and its temporal behavior was observed and analyzed through various statistical tests. Among major ions, the SO_4^(2-)has the highest concentration among anions on an average considered as 14.21% in 2013 and 29.46% in 2015. On the other side Ca^(2+) is the dominant cation contributing 28.22% in 2013 and 15.3% in 2015 on average. The average ratio of Na+/Cl-was higher in 2013 whereas lower in 2015. The backward trajectory analysis suggests the possible sources of the ions transported from Central Asia through the Western Disturbance(WD) as a prominent source of winter precipitation mainly in the Central Himalaya. Ionicconcentration of Ca^(2+) in cations was highly dominated while in anion SO_4^(2-)played the major role. Factor analysis and correlation matrix suggested that, the precipitation chemistry is mostly influenced by anthropogenic, crustal, and sea salt sources over the studied region. The elemental cycling through ocean, atmosphere and biosphere opens up new ways to understand the geochemical processes operating at the glacierized catchments of the Himalaya. Moreover, increasing the field-based studies in the coming decades would also have the certain advantage in overcoming the conceptual and computational geochemical modelling difficulties.
基金Supported by the National High Technology Research and Development Program of China (2006AA02Z246 2007AA03Z456) the National Natural Science Foundation of China (20776119 21076169)+4 种基金 Xi’an Research and Development Program(CX0735) the Scientific Research Program of Shaanxi Provincial Department of Education China (07JK417 07JC16 JG08181) the Natural Science Foundation of Shaanxi Province (2010JQ2012) the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023 20096101110014) Shaanxi Key Subject Program China
文摘Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production and the lowest production of some hazardous by-products, especially acetic acid, were obtained in the media containing 0.085 mol·L-1 glucose and 0.019 mol·L-1 nitrogen (carbon-nitrogen ratio, 4.47:1). After thermal induction, when the concentrations of glucose and nitrogen in the media were 0.065 mol·L-1 and 0.017 mol·L-1 , respectively (carbon-nitrogen ratio, 3.82:1), the productivity of human-like collagen per cell was the highest while that of acetic acid was the lowest. The extended analysis showed that the production of lactic acid and propionic acid increased while that of some intermediate acids of the tricarboxylic acid cycle decreased if the dose of glucose increased.
基金Supported by Grants from the National Natural Science Foundation of China,No.30200269Science and technology projects in Shannxi Province,No.2006k12-G3(6)Science and technology projects in Xi’an city,No.SF1025(3)
文摘AIM: To investigate the relationship between donor liver cold preservation, lung surfactant (LS) changes and acute lung injury (ALI) after liver transplantation. METHODS: Liver transplantation models were estab- lished using male Wistar rats. Donor livers were pre- served in University of Wisconsin solution at 4 ℃ for different lengths of time. The effect of ammonium pyr- rolidinedithiocarbamate (PDTC) on ALI was also detect- ed. All samples were harvested after 3 h reperfusion.The severity of AU was evaluated by lung weight/body weight ratio, lung histopathological score, serum nitric oxide (NO) and endothelin (ET)-I levels, lung tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels. Lung surfactants (LSs) were determined by micellar electrokinetic capillary chromatography. RESULTS: With extended donor liver cold preservation time (CPT), lung histopathological scores, serum ET-i levels, lung weight/body weight ratio and the level of TNF-α and IL-1β in lung were increased significantly in the 180-min group compared with the sham group (3.16± 0.28 vs 1.12 ± 0.21, P 〈 0.001; 343.59±53.97 vs 141.53± 48.48, P 〈 0.001; 0.00687 ±0.00037 vs 0.00557 ±0.00056, P 〈 0.001; 17.5 ± 3.0 vs 1,3 ± 0.3, P 〈 0.001; 10.8± 2.3 vs 1.8 ± 0.4, P 〈 0.001), but serum NO levels decreased remarkably (74.67 ± 10.01 vs 24.97 ± 3.18, P 〈 0.001). The expression of lung phos- phatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) and phosphatidylserine (PS) increased when CPT was 〈 120 rain, and decreased when CPT was 〉 180 min (PC: 1318.89 ± 54.79 vs 1011.18± 59.99, P 〈 0.001; PE: 1504.45± 119.96 vs 1340.80±76.39, P = 0.0019; PI: 201.23 ± 34.82 vs 185.88 ± 17.04, P = 0.2265; PS: 300.43±32.95 vs 286.55± 55.55, P = 0.5054). All these ALI-associated indexes could be partially reversed by PDTC treatment.
基金National Natural Science Foundation of China(No.61376052)State Key Laboratory Program(No.SKT1507)
文摘To enlarge the detection range of traditional fiber-optic spectrometer,two rotating parabolic mirrors were first used in the design of fiber-optic collector for spectrometer,which can extend the detection range of spectrometer from centimeter-scale to50m,as a super-low altitude remote sensing detector under passive lighting conditions.According to the performance requirements of fiber-spectrometer for optical collector,this study deduced the calculation formulae of design size of parabolic optical collector applied to different sensitivity spectrometers,different detection targets,different detection ranges and different weathers.Based on the calculation results,the model of fiber-optic collector with a diameter of16mm and a height of7.8mm was designed by ZEMAX.And then,TracePro,a light simulation software,was applied to ray tracing.The simulation results of optical radiation magnification agrees well with theoretical value,and the relative error is less than0.78%.This optical collector can effectively extend the detection range of spectrometer and expand its application fields and scopes.Compared with traditional refractive optical collector,it has several advantages of compact structure,low weight and low cost.
基金Under the auspices of National Natural Science Foundation of China (No.40876010)Key Direction in Knowledge Innovation Programs of Chinese Academy of Sciences (No. KZCX2-YW-Q03-08)+2 种基金Research and Development Special Fund for Public Welfare Industry (Meteorology) (No. GYHY200806010)LASG State Key Laboratory Special Fund, Foundation of E-Institutes of Shanghai Municipal Education Commission (No.E03004)Natural Science Foundation of Education Department of Fujian Province (No.JA10288)
文摘A coupled system of the interdecadal sea-air oscillator model is studied. The E1 Nifio-southem oscillation (ENSO) atmospheric physics oscillation is an abnormal phenomenon involved in the tropical Pacific ocean-atmosphere interactions. The oscillator model is involved with the variations of both the eastern and western Pacific anomaly pat- terns. This paper proposes an ENSO atmospheric physics model using a method of the perturbation theory. The aim is to create an asymptotic solving method for the ENSO model. Employing the perturbed method, the asymptotic solution of corresponding problem is obtained, and the asymptotic behaviour of the solution is studied. Thus we can obtain the prognoses of the sea surface temperature anomaly and related physical quantities.
文摘The flesh of spaghetti squash separates into strands when cooked. The purpose of this paper is to investigate the cause of strand separation (during cooking) by soaking for 24 h at 35 ℃ in solutions with three kinds of pectin extractant. The changes in strand separation, firmness, histological structure and the pectin of flesh during soaking in 0.01 N HCI solution (pH 2.0), 0.035 M ammonium oxalate solution (pH 4.0) or 2% sodium hexametaphosphate solution (pH 4.0) were investigated. When flesh was soaked in the HCI solution, the separation into strands and removal of calcium and magnesium were greater than that soaked in other pectin extractants. High methoxyl pectin was extracted by soaking in HC1 solution (pH 2.0) due to removal of polyvalent cations. This result shows that high methoxyl pectin glues strands together in the flesh of spaghetti squash. The shape of the cells which constituted strands was round; on the other hand, that of cells surrounded strands was elongated. When cooked in boiling water or soaked at pH 2.0, the shape of the former cells was maintained, but the latter cells, which contributed to adhesion between strands, broke down. Thus, the flesh separated into strands. When flesh was boiled for 15-30 min, pectin degraded and dissolved in the cooking solution; consequently, the flesh separated into strands and also the middle lamella of cell walls of strands separated. However, pectin remaining in strands maintained their crispness.
文摘The purpose of this paper is to investigate the relationship between pectic substances and the separation into strands during cooking of spaghetti squash. Spaghetti squash flesh separated into strands when boiled. Pectic substances of raw and cooked flesh were fractionated into three reagents. The galacturonic acid compositions of hydrochloric acid (HC1)-soluble pectin (PA), sodium acetate buffer-soluble pectin (PB) and sodium hexametaphosphate-soluble pectin (PC) of raw flesh were 69.0%, 28.9% and 2.1%, respectively. Also, the degree of esterification (DE) ofPA, PB and PC was 67.4%, 61.5% and 55.6%, respectively. The DE of pectin was from the greatest to the least: PA 〉 PB 〉 PC, respectively, and the diethylaminoethyl (DEAE)-cellulose column chromatograms of PA and PB showed that they were comparatively in high methoxyl pectin. Therefore, about 50% of pectic substances in flesh were released into a cooking solution during 15-30 min of cooking. Highmethoxylpectin was degraded by β-elimination during boiling. Consequently, the flesh was separated into strands. This suggests that highmethoxylpectin glues strands together in the flesh of spaghetti squash.
文摘Studying the ecology of ants can be a powerful tool for conservation, While the effect of logging is mainly investigated by the comparison of species richness and composition, the impact on individual species are often neglected. This study investigated the effect of selective logging on the nest density, foraging range and colony size on the ground-dwelling ant Aphaenogaster swammerdami in Kirindy forest--Madagascar. This ant is a common ground-dwelling species in Kirindy, a western dry deciduous forest of Madagascar. Sampling was done in two sites of the forest: One part that was selectively lodged and another that have not been logged. Here we show that selective logging led to a decrease in colony size and density, while the foraging range seemed to be unaffected. Higher desiccation stress and lower food availability in the logged forest are most likely to be responsible for these results.
基金Supported by the National Natural Science Foundation of China(No.61602477)China Postdoctoral Science Foundation(No.2016M601158)National Key Research and Development Program of China(No.2016YFB0200804)
文摘The future climate dynamical downscaling method is that output of general circulation models( GCMs) is employed to provide initial conditions,lateral boundary conditions,sea surface temperatures,and initial land surface conditions to regional climate models( RCMs). There are two methods of downscaling: offline coupling and online coupling. The two kinds of coupling methods are described in detail by coupling the Weather Research and Forecasting model( WRF) with the Institute of Atmospheric Physics of Chinese Academy of Sciences Atmospheric General Circulation Model Version 4. 0( IAP AGCM4. 0) in the study. And the extreme precipitation event over Beijing on July 212012 is simulated by using the two coupling methods. Results show that online coupling method is of great value in improving the model simulation. Furthermore,the data exchange frequency of online coupling has some effect on simulation result.
文摘This research was carried out to study the effect of fermentation on the chemical composition, anti-nutrient content, PH, titratable acidity, and microbiological changes of millet and soyabean blend. Millet and soyabean composite flours were mixed in gram of six combinations as follows millet and soyabean (A) = 100:0, millet and soyabean (B) = 90:10, millet and soyabean (C) = 80:20, millet and soyabean (D) = 70:30, millet and soyabean (E) = 60:40, millet and soyabean (F) = 50:50 and subjected to natural fermentation for 72 h. The following bacteria isolates were obtained from the fermentation; Lacobacillus fermentum, L. acidophilus, L. bulgaricus, L. plantarum, L. dextranicum, L. rhamnosus, L. delbrueckii, L. leichemanii, L. divergens, L. reuteri, L. jenseni, L. casei, L. salivarius, L. cellubiosus, Leuconostoc mesenteroide and Pediococcus acidilactis, of which L. plantarum was the most dominant the throughout the period of fermentation. There was decrease in pH with increase in TTA in all the samples. The result of the proximate analysis revealed a marginal increase in crude protein content of each sample (from 44.41 to 63.14, from 11.02 to 24.02, from 16.64 to 23.10, from 20.83 to 26.93, from 25.43 to 30.12, 39.12 to 35.86 and from 40.66 to 54.24%) There was increase in ash content and decrease in carbohydrate, fibre and fat contents of the fermented samples. Results from this research also show significant reduction in anti-nutritional content which are hydrogen cyanide, oxalate and phytate. Fermentation has modified the microbial and nutritional quality of the millet and soyabean blend and this has greatly improved the nutrient content of the blend.
基金Projects(50671005,50971093)supported by the National Natural Science Foundation of ChinaProject(2007CB613705)supported by the National Basic Research Program of China
文摘Corrosion behavior of AZ91 magnesium alloy under NaCl particle deposition condition was investigated by gravimetric method and surface analysis technique.It was found that the mass gain increased rapidly at the beginning of exposure and then slowly with time.The corrosion morphologies were observed and the results showed that NaCl deposition resulted in the occurrence of localized corrosion.The composition of corrosion product was analyzed using X-ray photo electron spectroscopy.It was suggested that the corrosion product was a mixture of oxide and hydroxide of magnesium and aluminum.
文摘 The effect of Batroxobin on spatial memory disorder of left temporal ischemic rats and the expression of HSP32 and HSP70 were investigated with Morri`s water maze and immunohistochemistry methods. The results showed that the mean reaction time and distance of temporal ischemic rats in searching a goal were significantly longer than those of the sham-operated rats and at the same time HSP32 and HSP70 expression of left temporal ischemic region in rats was significantly increased as compared with the sham-operated rats. However, the mean reaction time and distance of the Batroxobin-treated rats were shorter and they used normal strategies more often and earlier than those of ischemic rats. The number of HSP32 and HSP70 immune reactive cells of Batroxobin-treated rats was also less than that of the ischemic group. In conclusion, Batroxobin can improve spatial memory disorder of temporal ischemic rats; and the down-regulation of the expression of HSP32 and HSP70 is probably related to the attenuation of ischemic injury.
文摘Purpose: To evaluate the effect of a hydroethanolic extract of Moltkia coerulea ointment (MCO) on the healing of excision wound in a rat model. Methods: Circular surgical full thickness excision wound, with 314 mm2 size, was induced in the anterior-dorsal side of each rat. Three different doses of MCO (1%, 3% and 6%) were administrated. On Day 3, 7, 14 and 21, the tissue was sampled and immune cells, fibroblasts and fibrocytes distribution per one mm2 of wound area, collagen density and re-epithelialization were analyzed. Moreover, the total flavnoid, phenols and anti-oxidant potential of the MCO were evaluated. Ultimately, the percentage of wound contraction in different groups was compared with each other. Results: Hydroethanolic extract of MCO significantly (p 〈 0.05) increased wound contraction percentage. The animals in medium and high dose MCO-treated groups exhibited remarkably (p 〈 0.05) higher fibroblast and fibrocyte distribution and signifcantly (p 〈 0.05) lower immune cells infiltration. On Day 7 after injury, MCO up-regulated neovascularization in a dose-dependent way. Conclusion: Our data showed that MCO shortened the inflammation phase by provoking the fibroblast proliferation. Moreover, MCO promoted the healing process by up-regulating the angiogenesis and provoking the structural cells proliferation as well as increasing the collagen synthesis, cross-linking, and deposition.
文摘The paper studies the ground vertical deformation and the geoid undulation caused by loading of neighboring buildings, based on the loading tides theory. The influence on elevation is also considered. The results show that the ground vertical deformation and the geoid undulation both reach millimeter magnitude. Therefore, it is obvious that the building loading significantly affects the precise engineering surveying, and it must be seriously considered in application.