Chemical communication plays an important role in survival and reproductive success in mammalian species. In the present study, we examined the ontogenetic pattern of behavioral responses of male giant pandas (Ailuro...Chemical communication plays an important role in survival and reproductive success in mammalian species. In the present study, we examined the ontogenetic pattern of behavioral responses of male giant pandas (Ailuropoda melanoleuca ) to urine odors of conspecific individuals. Our data showed that exposure to the urine of adult females induced a significant increase in sniffing and environmental sniffing/licking behaviors, but a decrease in biting behavior, in males. Males of different ages displayed specific behaviors to female urine odors. Adult males spent more time licking than juvenile and sub-adult males. Further, sub-adult and adult males displayed high levels of environmental sniffing/licking, which was absent in the juvenile males. Juvenile males displayed scent rubbing behavior significantly more frequently than sub-adult and adult males, and also spent more time showing biting behavior than sub-adult males. Finally, juvenile and sub-aduh males showed no difference in response to female and male urine odors. Together, these data suggest that chemosensory cues from conspecific urines induce age-specific responses in male giant pandas.展开更多
In this study, non radioactive Digoxigenin labeled ribosomal DNA(rDNA) probes were used for Southern blotting analysis to study the molecular phylogeny of the giant panda and related species. Restriction maps in the ...In this study, non radioactive Digoxigenin labeled ribosomal DNA(rDNA) probes were used for Southern blotting analysis to study the molecular phylogeny of the giant panda and related species. Restriction maps in the regions of rDNA spacers were compared between giant panda( Ailuropoda melanoleuca ), lesser panda( Ailurus fulgens ), Asiatic black bear( Selenarctos thibetanus ), sun bear( Helarctos malayanus ), raccoon( Procyon lotor ) and lynx( Felis lynx ). Phylogenetic trees for these species were constructed using maximum likelihood and parsimony method. The results show that in respect to rDNA RFLPs, the giant panda is more closely related to bear than to lesser panda; while the lesser panda is slightly related to the raccoon.展开更多
In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using t...In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.展开更多
The goal of the giant panda ( Ailuropoda melanoleuca ) breeding program is to develop a self sustaining,genetically diverse population.Due to the common problems about sexual incompatibility and a limited number of...The goal of the giant panda ( Ailuropoda melanoleuca ) breeding program is to develop a self sustaining,genetically diverse population.Due to the common problems about sexual incompatibility and a limited number of captive born males that breed naturally,artificial insemination (AI) has become a critical genetic management tool.It is common practice,however,to combine natural mating and AI using semen from non breeding males.From 1998 to 2000 at the Wolong breeding facility,12 of 18 (66.7%) females produced 20 cubs following combined natural mating and AI.The objective of this study was to determine the efficiency of AI without natural breeding.In 1998 and 2000,seven females were anesthetized for transcervical AI on two consecutive days.Ejaculates from six males were collected by electroejaculation,diluted in an egg yolk diluent containing 0% or 4% glycerol and used either fresh or following cold storage at 4℃ (for 24 or 48 h) or cryopreservation using the pellet freezing method.Mean (±SEM) ejaculate traits in six male sperm donors were:ejaculate volume,3.3±0.5 ml;sperm concentration,1,429.8±235.4×10 6/ml;sperm motility,81.7±2.1%;progression (0~5,5=best),3.1±0.1;and normal sperm,79.3±9.2%.For AI (n = 14) in seven females,mean inseminate traits were:spermic volume inseminated,2.4±0.3 ml;sperm motility,73.5±2.9%;progression,2.5±0.1;and total motile sperm inseminated/AI,684.2±118.2×10 6.Four of seven (57.1%) females became pregnant and produced five cubs of which four survived.Mean gestation and litter size was 131.5±9.7 days and 1.3±0.3 cubs/litter,respectively.These results indicate that the efficiency of AI is sufficient for recovering valuable genes from non breeding individuals to enhance genetic diversity in the ex situ population of giant pandas.展开更多
The Wenchuan Earthquake that occurred in May of 2008 caused damages to large areas of Sichuan,Gansu,and Shaanxi provinces in China.Reports from local governments and related management agencies show that the giant pan...The Wenchuan Earthquake that occurred in May of 2008 caused damages to large areas of Sichuan,Gansu,and Shaanxi provinces in China.Reports from local governments and related management agencies show that the giant panda nature reserves in the earthquake-hit areas were heavily damaged.Our estimates in this paper of the impacts of the earthquake on the giant panda in the earthquake-hit areas were made based on the interpretation of remote sensing images and information collected by field survey.A rapid assessment method was designed to estimate the damages of the earthquake on giant panda habitats.By using visual interpretation methods,we decoded the remote sensing images of the disaster area in the 49 giant panda nature reserves.Research results showed that the Wenchuan Earthquake and the succeeding secondary geological disasters caused great damages to the giant panda nature reserves and disturbed the normal life of the giant pandas there (e.g.,landscape fragmentation increased significantly).Undoubtedly,the life of the giant pandas there was affected.However,although the earthquake caused certain impacts on the giant pandas,it did not really threat their survival.Even so,we still strongly advocate for protection of the giant pandas,and have prioritized a couple of measures to be taken to restore the giant panda nature reserves in the earthquake-hit areas.展开更多
Many nature reserves are established to protect the habitat needs of particular endangered species of interest but their effectiveness for protecting other species is questionable.In this study,this effectiveness was ...Many nature reserves are established to protect the habitat needs of particular endangered species of interest but their effectiveness for protecting other species is questionable.In this study,this effectiveness was evaluated in a nature reserve network located in the Qinling Mountains,Shaanxi Province,China.The network of reserves was established mainly for the conservation of the giant panda,a species considered as a surrogate for the conservation of many other endangered species in the region.The habitat suitability of nine protected species,including the giant panda,was modeled by using Maximum Entropy(MAXENT)and their spatial congruence was analyzed.Habitat suitability of these species was also overlapped with nature reserve boundaries and their management zones(i.e.,core,buffer and experimental zones).Results show that in general the habitat of the giant panda constitutes a reasonable surrogate of the habitat of other protected species,and giant panda reserves protect a relatively high proportion of the habitat of other protected species.Therefore,giant panda habitat conservation also allows the conservation of the habitat of other protected species in the region.However,a large area of suitable habitat was excluded from the nature reserve network.In addition,four species exhibited a low proportion of highly suitable habitat inside the core zones of nature reserves.It suggests that a high proportion of suitable habitat of protected species not targeted for conservation is located in the experimental and buffer zones,thus,is being affected by human activities.To increase their conservation effectiveness,nature reserves and their management zones need to be re-examined in order to include suitable habitat of more endangered species.The procedures described in this study can be easily implemented for the conservation of many endangered species not only in China but in many other parts of the world.展开更多
In this study,we used remotely sensed data,GIS modeling,and statistical methods to evaluate the damage caused by the Wenchuan Earthquake (May 12,2008) to the giant panda (Ailuropoda melanoleuca) habitat in the World N...In this study,we used remotely sensed data,GIS modeling,and statistical methods to evaluate the damage caused by the Wenchuan Earthquake (May 12,2008) to the giant panda (Ailuropoda melanoleuca) habitat in the World Nature Heritage Sichuan Giant Panda Sanctuary (WHSGPS) in China.A landscape ecological analysis found increases of landscape heterogeneity,complexity,and fragmentation in the giant panda habitat after the earthquake.A terrain analysis found that slope and elevation are directly associated with the distribution of the damaged areas.As slope and elevation increase,the size of the damaged area keeps increase until to a peak,and then starts to drop.The total area of the damaged vegetation in our study area is 114.26 km 2,accounting for 3.78% of the study area;30.46% of that 114.26 km 2 is located in the Core Zone of WHSGPS.There are 18.57km 2 of the damaged vegetation located in the identified suitable giant panda habitat,accounting for 1.75% of the total area of suitable giant panda habitats in the study area.Based on these findings,we conclude that the Wenchuan Earthquake does not have significant impact on the WHSGPS as a whole.展开更多
The endangered giant panda (Ailuropoda melanoleuca) is endemic to th e mountains of Sichuan, Gansu and Shaanxi Provinces in China. The species had nu mbered over 2000 animals in early 1970s, but declined to no more th...The endangered giant panda (Ailuropoda melanoleuca) is endemic to th e mountains of Sichuan, Gansu and Shaanxi Provinces in China. The species had nu mbered over 2000 animals in early 1970s, but declined to no more than 1,000 anim als fragmented into perhaps 32 subpopulations in late 1990s as the result of num erous detrimental forces such as habitat shrinking, poaching and bamboo flowerin g. The mass flowering and followed die-off of bamboo played key roles in the dec lination of panda population in the past three decades. It trigged the starvatio n and following high mortality of giant pandas in 1970s over Min Mountains and i n 1980s across Qionglai Mountains in Sichuan Province. The situation of survivor ship is made worse by the fact that the panda habitat is fragmented into many sm all 'islands', each containing only a few pandas. Such small, isolated panda pop ulations are rendered even more vulnerable to extinction form threats such as ha bitat degradation, natural disaster, disease, and the deleterious effects of inb reeding. So restoring the panda habitat and reintroduction pandas to their histo rical habitat might be an important issue for protecting the giant panda in wild .展开更多
Seasonal changes of progesterone content (PSC) in hair of three female giant pandas and testosterone content (TSC) in hair of two male giant pandas were tested. It was found that the two hormones could be detected by ...Seasonal changes of progesterone content (PSC) in hair of three female giant pandas and testosterone content (TSC) in hair of two male giant pandas were tested. It was found that the two hormones could be detected by radioimmunoassay in all the hair samples scissored from the female and male giant pandas respectively. During March to early June, PSC in two non-pregnent giant pandas (±SD = 13.40 ± 10.06 and10.60±8.88 ng / g hair respectively) were higher than those in non-breeding season (3.07 ± 1.07 and 3.20 ±1.15 ng / g, P< 0.01). PSC in a 18--year--old female giant panda remained at low levels (2.72 ± 1.49 ng / g) during March to December. In a twin--beargiant panda, PSC (6.77± 3.66 ng / g) appeared higher than that.in non--pregnant giantpandas in non--breeding season. Around February tO the end of May, TSC in two male giant pandas (1.89± 1.71 and 1.82 ± 1.04 ng / g respectively) were also higher than that in non-breeding season (0.98 ± 0.57 and 0.75 ± 0.39 ng / g, P< 0.01). The findings from the study implied that giant panda's hair is possible to be used as a specimen tO carryout steroids research in the endangered species.展开更多
Environmental enrichment increases the expression of behavior and welfare. The aim of the study was to analyze situation of the social enrichment (training) of big cats and cheetahs in Czech and Slovak zoos based on...Environmental enrichment increases the expression of behavior and welfare. The aim of the study was to analyze situation of the social enrichment (training) of big cats and cheetahs in Czech and Slovak zoos based on the evaluation questionnaire. It was found out that social enrichment, as part of the environmental enrichment, was used in 50% of animal, 16 animal get training only. A lot of animal had positive reactions on enrichment.展开更多
“Bamboo has blossomed. Held in her mother’s arms, Mimi is counting the number of stars in the sky. How beautiful the stars are and where is my breakfast tomorrow...
A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T gr...A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T grew optimally at pH 7.0-8.0, at 28-30 ℃ and in the presence of NaCI concentrations from 0.0% to 0.5%. 16S rRNA gene sequence analysis indicated that strain 25-1m belongs to the genus Chryseobacterium within the family Flavo- bacteriaceae and is related most closely to C. camis G81m (96.4% similarity), C. lathyri RBA2-6T (95.8% similarity), and C. zeae JM1085T (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C15:0 (44.0%), iso-C17:0 3OH (19.8%) and C16:1 ω7c/16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA-DNA relatedness between strain 25-1m and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T (ccmoc AB2015133T=DSM 100396T).展开更多
文摘Chemical communication plays an important role in survival and reproductive success in mammalian species. In the present study, we examined the ontogenetic pattern of behavioral responses of male giant pandas (Ailuropoda melanoleuca ) to urine odors of conspecific individuals. Our data showed that exposure to the urine of adult females induced a significant increase in sniffing and environmental sniffing/licking behaviors, but a decrease in biting behavior, in males. Males of different ages displayed specific behaviors to female urine odors. Adult males spent more time licking than juvenile and sub-adult males. Further, sub-adult and adult males displayed high levels of environmental sniffing/licking, which was absent in the juvenile males. Juvenile males displayed scent rubbing behavior significantly more frequently than sub-adult and adult males, and also spent more time showing biting behavior than sub-adult males. Finally, juvenile and sub-aduh males showed no difference in response to female and male urine odors. Together, these data suggest that chemosensory cues from conspecific urines induce age-specific responses in male giant pandas.
文摘In this study, non radioactive Digoxigenin labeled ribosomal DNA(rDNA) probes were used for Southern blotting analysis to study the molecular phylogeny of the giant panda and related species. Restriction maps in the regions of rDNA spacers were compared between giant panda( Ailuropoda melanoleuca ), lesser panda( Ailurus fulgens ), Asiatic black bear( Selenarctos thibetanus ), sun bear( Helarctos malayanus ), raccoon( Procyon lotor ) and lynx( Felis lynx ). Phylogenetic trees for these species were constructed using maximum likelihood and parsimony method. The results show that in respect to rDNA RFLPs, the giant panda is more closely related to bear than to lesser panda; while the lesser panda is slightly related to the raccoon.
基金This research was supported by National Science Founda-tion of China (No. 30000123) and Conversation Department of Wildlife Ani-mal & Plants of State Forestry Bureau.
文摘In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.
文摘The goal of the giant panda ( Ailuropoda melanoleuca ) breeding program is to develop a self sustaining,genetically diverse population.Due to the common problems about sexual incompatibility and a limited number of captive born males that breed naturally,artificial insemination (AI) has become a critical genetic management tool.It is common practice,however,to combine natural mating and AI using semen from non breeding males.From 1998 to 2000 at the Wolong breeding facility,12 of 18 (66.7%) females produced 20 cubs following combined natural mating and AI.The objective of this study was to determine the efficiency of AI without natural breeding.In 1998 and 2000,seven females were anesthetized for transcervical AI on two consecutive days.Ejaculates from six males were collected by electroejaculation,diluted in an egg yolk diluent containing 0% or 4% glycerol and used either fresh or following cold storage at 4℃ (for 24 or 48 h) or cryopreservation using the pellet freezing method.Mean (±SEM) ejaculate traits in six male sperm donors were:ejaculate volume,3.3±0.5 ml;sperm concentration,1,429.8±235.4×10 6/ml;sperm motility,81.7±2.1%;progression (0~5,5=best),3.1±0.1;and normal sperm,79.3±9.2%.For AI (n = 14) in seven females,mean inseminate traits were:spermic volume inseminated,2.4±0.3 ml;sperm motility,73.5±2.9%;progression,2.5±0.1;and total motile sperm inseminated/AI,684.2±118.2×10 6.Four of seven (57.1%) females became pregnant and produced five cubs of which four survived.Mean gestation and litter size was 131.5±9.7 days and 1.3±0.3 cubs/litter,respectively.These results indicate that the efficiency of AI is sufficient for recovering valuable genes from non breeding individuals to enhance genetic diversity in the ex situ population of giant pandas.
基金supported by the Ministry of Science and Technology of China (2008BAK47B022008BAC44B04+2 种基金2008BAK50B062008BAC43B012006BAC08B06)
文摘The Wenchuan Earthquake that occurred in May of 2008 caused damages to large areas of Sichuan,Gansu,and Shaanxi provinces in China.Reports from local governments and related management agencies show that the giant panda nature reserves in the earthquake-hit areas were heavily damaged.Our estimates in this paper of the impacts of the earthquake on the giant panda in the earthquake-hit areas were made based on the interpretation of remote sensing images and information collected by field survey.A rapid assessment method was designed to estimate the damages of the earthquake on giant panda habitats.By using visual interpretation methods,we decoded the remote sensing images of the disaster area in the 49 giant panda nature reserves.Research results showed that the Wenchuan Earthquake and the succeeding secondary geological disasters caused great damages to the giant panda nature reserves and disturbed the normal life of the giant pandas there (e.g.,landscape fragmentation increased significantly).Undoubtedly,the life of the giant pandas there was affected.However,although the earthquake caused certain impacts on the giant pandas,it did not really threat their survival.Even so,we still strongly advocate for protection of the giant pandas,and have prioritized a couple of measures to be taken to restore the giant panda nature reserves in the earthquake-hit areas.
基金Under the auspices of National Natural Science Foundation of China(No.40901289)Major State Basic Research Development Program of China(No.2009CB421104),U.S.National Science Foundation
文摘Many nature reserves are established to protect the habitat needs of particular endangered species of interest but their effectiveness for protecting other species is questionable.In this study,this effectiveness was evaluated in a nature reserve network located in the Qinling Mountains,Shaanxi Province,China.The network of reserves was established mainly for the conservation of the giant panda,a species considered as a surrogate for the conservation of many other endangered species in the region.The habitat suitability of nine protected species,including the giant panda,was modeled by using Maximum Entropy(MAXENT)and their spatial congruence was analyzed.Habitat suitability of these species was also overlapped with nature reserve boundaries and their management zones(i.e.,core,buffer and experimental zones).Results show that in general the habitat of the giant panda constitutes a reasonable surrogate of the habitat of other protected species,and giant panda reserves protect a relatively high proportion of the habitat of other protected species.Therefore,giant panda habitat conservation also allows the conservation of the habitat of other protected species in the region.However,a large area of suitable habitat was excluded from the nature reserve network.In addition,four species exhibited a low proportion of highly suitable habitat inside the core zones of nature reserves.It suggests that a high proportion of suitable habitat of protected species not targeted for conservation is located in the experimental and buffer zones,thus,is being affected by human activities.To increase their conservation effectiveness,nature reserves and their management zones need to be re-examined in order to include suitable habitat of more endangered species.The procedures described in this study can be easily implemented for the conservation of many endangered species not only in China but in many other parts of the world.
基金supported by Sichuan Foundation of Excellent Scientists (Grant No.2010JZ0002)the Directional Project of Chinese Academy of Sciences (Grant No.KZX2-YW-333)
文摘In this study,we used remotely sensed data,GIS modeling,and statistical methods to evaluate the damage caused by the Wenchuan Earthquake (May 12,2008) to the giant panda (Ailuropoda melanoleuca) habitat in the World Nature Heritage Sichuan Giant Panda Sanctuary (WHSGPS) in China.A landscape ecological analysis found increases of landscape heterogeneity,complexity,and fragmentation in the giant panda habitat after the earthquake.A terrain analysis found that slope and elevation are directly associated with the distribution of the damaged areas.As slope and elevation increase,the size of the damaged area keeps increase until to a peak,and then starts to drop.The total area of the damaged vegetation in our study area is 114.26 km 2,accounting for 3.78% of the study area;30.46% of that 114.26 km 2 is located in the Core Zone of WHSGPS.There are 18.57km 2 of the damaged vegetation located in the identified suitable giant panda habitat,accounting for 1.75% of the total area of suitable giant panda habitats in the study area.Based on these findings,we conclude that the Wenchuan Earthquake does not have significant impact on the WHSGPS as a whole.
文摘The endangered giant panda (Ailuropoda melanoleuca) is endemic to th e mountains of Sichuan, Gansu and Shaanxi Provinces in China. The species had nu mbered over 2000 animals in early 1970s, but declined to no more than 1,000 anim als fragmented into perhaps 32 subpopulations in late 1990s as the result of num erous detrimental forces such as habitat shrinking, poaching and bamboo flowerin g. The mass flowering and followed die-off of bamboo played key roles in the dec lination of panda population in the past three decades. It trigged the starvatio n and following high mortality of giant pandas in 1970s over Min Mountains and i n 1980s across Qionglai Mountains in Sichuan Province. The situation of survivor ship is made worse by the fact that the panda habitat is fragmented into many sm all 'islands', each containing only a few pandas. Such small, isolated panda pop ulations are rendered even more vulnerable to extinction form threats such as ha bitat degradation, natural disaster, disease, and the deleterious effects of inb reeding. So restoring the panda habitat and reintroduction pandas to their histo rical habitat might be an important issue for protecting the giant panda in wild .
文摘Seasonal changes of progesterone content (PSC) in hair of three female giant pandas and testosterone content (TSC) in hair of two male giant pandas were tested. It was found that the two hormones could be detected by radioimmunoassay in all the hair samples scissored from the female and male giant pandas respectively. During March to early June, PSC in two non-pregnent giant pandas (±SD = 13.40 ± 10.06 and10.60±8.88 ng / g hair respectively) were higher than those in non-breeding season (3.07 ± 1.07 and 3.20 ±1.15 ng / g, P< 0.01). PSC in a 18--year--old female giant panda remained at low levels (2.72 ± 1.49 ng / g) during March to December. In a twin--beargiant panda, PSC (6.77± 3.66 ng / g) appeared higher than that.in non--pregnant giantpandas in non--breeding season. Around February tO the end of May, TSC in two male giant pandas (1.89± 1.71 and 1.82 ± 1.04 ng / g respectively) were also higher than that in non-breeding season (0.98 ± 0.57 and 0.75 ± 0.39 ng / g, P< 0.01). The findings from the study implied that giant panda's hair is possible to be used as a specimen tO carryout steroids research in the endangered species.
文摘Environmental enrichment increases the expression of behavior and welfare. The aim of the study was to analyze situation of the social enrichment (training) of big cats and cheetahs in Czech and Slovak zoos based on the evaluation questionnaire. It was found out that social enrichment, as part of the environmental enrichment, was used in 50% of animal, 16 animal get training only. A lot of animal had positive reactions on enrichment.
文摘“Bamboo has blossomed. Held in her mother’s arms, Mimi is counting the number of stars in the sky. How beautiful the stars are and where is my breakfast tomorrow...
基金supported by The National Basic Research Development Program of China(No.2009CB42115)the National Natural Science foundation of China(No.40901289),and WWF China
基金supported by the Chengdu Giant Panda Breeding Research Foundation(No.CPF2010-06)the National Key Technology R&D Program of China(No.2012BAC01B06)
文摘A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T grew optimally at pH 7.0-8.0, at 28-30 ℃ and in the presence of NaCI concentrations from 0.0% to 0.5%. 16S rRNA gene sequence analysis indicated that strain 25-1m belongs to the genus Chryseobacterium within the family Flavo- bacteriaceae and is related most closely to C. camis G81m (96.4% similarity), C. lathyri RBA2-6T (95.8% similarity), and C. zeae JM1085T (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C15:0 (44.0%), iso-C17:0 3OH (19.8%) and C16:1 ω7c/16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA-DNA relatedness between strain 25-1m and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T (ccmoc AB2015133T=DSM 100396T).