An entomopathogenic strain of Bacillus sp. was isolated from diseased red slug caterpillars of the leaf-feediug pest of tea, Eterusia magnifica, from the Darjeeling foothill region. Analysis of the bacterimn based on ...An entomopathogenic strain of Bacillus sp. was isolated from diseased red slug caterpillars of the leaf-feediug pest of tea, Eterusia magnifica, from the Darjeeling foothill region. Analysis of the bacterimn based on polyphasic approach such as growth phase, biochemical tests, whole body＂ protein, crystal protein profiles along with bioassay （i.e. LC50 and LT50 values） established it as a different strain but close to Bacillus thuringiensis kurstaki （Btk）, the commercial microbial pesticides of lepidopterans. Among biochemical parameters differences were noted between the new strain and Btk in ONPG, lysine decarboxylase, omithin decarboxylase, urease, nitrate reduction, V-P and glucose utilization tests. PAGE analysis of the whole body protein for the new strain recorded a 34 kDa band which was absent in Btk （used as reference）. Crystal protein profile of the newly isolated bacterial strain showed 53 and 49 kDa bands whereas in Btk only 52 kDa band was evident. Although the LC50 values of the new strain and Btk were close, their LT50 values were much different, the new strain showing a lower value than Btk. In light of the above differences and in absence of any report of entomopathogenic bacterial strain of E. magnifica, the isolated strain of Bacillus appeared to be new to science and hence was designated as RS01. The new strain opens up the possibility of its futttre use as microbial pesticide after standardizing its formulation and checking its safety aspects.展开更多
Alternative Low-Input Agriculture Systems （ALIAS） center advocates organic and sustainable food production system through farm/field demonstration and piloting. The center undertakes activities, including research a...Alternative Low-Input Agriculture Systems （ALIAS） center advocates organic and sustainable food production system through farm/field demonstration and piloting. The center undertakes activities, including research and development, extension and training, technology dissemination, linkaging and promotional activities. The study established a campus-based demonstration technology showcasing ALIAS to facilitate easy technology diffusion. The objectives of this research were to promote： （1） bio-fertilizers and plant-based pesticides in managing insect pests and diseases of organic vegetables and fruit crops; （2） lotus-tilapia integration as a new modality of farming system in frequently flooded areas; （3） honeybee as pollinators to enhance productivity of organic vegetable and fruit crops; （4） Adlai as intercrop to organic vegetable and fruit crops in support to rice sufficiency; （5） native chicken-coffee integration. The results show that： （1） microbial inoculants and plant-based pesticides are effective in controlling diseases and insect pest, respectively; （2） lotus integration in tilapia production system have no negative effect on the tilapia yield; （3） honeybees as pollinators enhances fruit setting by 42%-68% in different crops; （4） Adlai is a viable intercrop for organic vegetable and fruit crops; （5） native chicken integration was found to be beneficial in terms of soil fertility, insect pest control, natural weeding practices and cultivation in the coffee plantation.展开更多
文摘An entomopathogenic strain of Bacillus sp. was isolated from diseased red slug caterpillars of the leaf-feediug pest of tea, Eterusia magnifica, from the Darjeeling foothill region. Analysis of the bacterimn based on polyphasic approach such as growth phase, biochemical tests, whole body＂ protein, crystal protein profiles along with bioassay （i.e. LC50 and LT50 values） established it as a different strain but close to Bacillus thuringiensis kurstaki （Btk）, the commercial microbial pesticides of lepidopterans. Among biochemical parameters differences were noted between the new strain and Btk in ONPG, lysine decarboxylase, omithin decarboxylase, urease, nitrate reduction, V-P and glucose utilization tests. PAGE analysis of the whole body protein for the new strain recorded a 34 kDa band which was absent in Btk （used as reference）. Crystal protein profile of the newly isolated bacterial strain showed 53 and 49 kDa bands whereas in Btk only 52 kDa band was evident. Although the LC50 values of the new strain and Btk were close, their LT50 values were much different, the new strain showing a lower value than Btk. In light of the above differences and in absence of any report of entomopathogenic bacterial strain of E. magnifica, the isolated strain of Bacillus appeared to be new to science and hence was designated as RS01. The new strain opens up the possibility of its futttre use as microbial pesticide after standardizing its formulation and checking its safety aspects.
文摘Alternative Low-Input Agriculture Systems （ALIAS） center advocates organic and sustainable food production system through farm/field demonstration and piloting. The center undertakes activities, including research and development, extension and training, technology dissemination, linkaging and promotional activities. The study established a campus-based demonstration technology showcasing ALIAS to facilitate easy technology diffusion. The objectives of this research were to promote： （1） bio-fertilizers and plant-based pesticides in managing insect pests and diseases of organic vegetables and fruit crops; （2） lotus-tilapia integration as a new modality of farming system in frequently flooded areas; （3） honeybee as pollinators to enhance productivity of organic vegetable and fruit crops; （4） Adlai as intercrop to organic vegetable and fruit crops in support to rice sufficiency; （5） native chicken-coffee integration. The results show that： （1） microbial inoculants and plant-based pesticides are effective in controlling diseases and insect pest, respectively; （2） lotus integration in tilapia production system have no negative effect on the tilapia yield; （3） honeybees as pollinators enhances fruit setting by 42%-68% in different crops; （4） Adlai is a viable intercrop for organic vegetable and fruit crops; （5） native chicken integration was found to be beneficial in terms of soil fertility, insect pest control, natural weeding practices and cultivation in the coffee plantation.
