Thirty Wistar rats were randomly and evenly divided into control group, cerebral ischemia group and ischemia + electroacupuncture (EA) group. The bilateral common carotid arteries were occluded to induce acute cerebra...Thirty Wistar rats were randomly and evenly divided into control group, cerebral ischemia group and ischemia + electroacupuncture (EA) group. The bilateral common carotid arteries were occluded to induce acute cerebral ischemia. Nitric oxide (NO) and endothelin (ET)contents in the cerebral tissues and blood were measured under normal condition, immediately after ischemia and following EA. Results showed that after acute cerebral ischemia NO and ET contents in the cerebral tissues increased significantly (P<0.01) while serum ET increased and serum NO lowered obviously (P<0.05). Following EA of Baihui (GV 20) and Dazhui (GV 14), both NO and ET in cerebral tissues and serum turned to normal basically. It showed that EA could protect the cerebral tissues from injury induced by ischemia, NO and ET might participate in the modulation process of EA.展开更多
Summary : To observe the therapeutic effect of acupuncture plus medicament in the treatment of prostatic hyperplasia, Zhibian (BL 54) and Zhongji (CV 3) were deeply punctured with elongated needles and enema was ...Summary : To observe the therapeutic effect of acupuncture plus medicament in the treatment of prostatic hyperplasia, Zhibian (BL 54) and Zhongji (CV 3) were deeply punctured with elongated needles and enema was applied with self-prepared medicinal herbs. Then Qugu (CV2), Zhongliao(BL 33) and Xialiao(BL 34) were selected and connected with a herbal Ion Introduction Apparatus. The total effective rate was 92.4% in 41 cases. Elongated needle and herbal ion introduction can relieve the clinical symptoms of prostatic hyperplasia and improve the patients' life quality.展开更多
Objective: To investigate the effects of acupuncture on the number of associated phosphorylated proteins in brain tissues of middle cerebral artery occlusion (MCAO) rats, based on the protein microarray technique. ...Objective: To investigate the effects of acupuncture on the number of associated phosphorylated proteins in brain tissues of middle cerebral artery occlusion (MCAO) rats, based on the protein microarray technique. Methods: The MCAO model was prepared according to the modified occlusion method using occlusion lines. Forty healthy Sprague-Dawley (SD) rats were randomly divided into 4 groups using the lottery method: a sham operation group, a model group, a control point group and an acupoint group, with 10 rats in each group. Rats in the sham operation group and the model group only received binding without acupuncture. Rats in the acupoint group received acupuncture at Dazhui (GV 14), Baihui (GV 20) and Shuigou (GV 25); rats in the control point group received acupuncture at non-acupoint control points. The needle was twisted once for 1 min after insertion and another time in the middle of the 30 min needle retaining. Acupuncture was conducted once every 12 h for 6 consecutive times. At the end of the experiment, the neurological impairment score was collected, and cells of the ischemic brain tissues were extracted. The protein phosphorylation of the related signaling was detected using the 720 phosphorylated antibody microarray technique, and the differentially expressed proteins between groups were screened. Results: The neurological impairment scores after 72 h of treatment: compared with the sham operation group, the scores of the model group, the control point group and the acupoint group were significantly increased (P〈0.01); compared with the model group, the scores of the acupoint group and the control point group were significantly decreased (P〈0.01,P〈0.05); the score of the acupoint group was better than that of the control point group (P〈0.05). The results of the protein microarray: compared with the sham operation group, 48 proteins showed up-regulated phosphorylation (≥1.5 times) in the model group and the down-regulated was 28; compared with the model group, 35 proteins showed up-regulated phosphorylation in the control point group, and the down-regulated was 24. There were 29 proteins showing up-regulated phosphorylation in the acupoint group and the down-regulated was 51. The numbers of proteins involved in the function and signal transduction pathways were also different. Conclusion: Acupuncture at Dazhui (GV 14), Baihui (GV 20) and Shuigou (GV 25) can effectively repair brain injury. The ischemic injury of brain tissue may be caused by imbalance of a variety of proteins, and acupuncture can promote brain tissue repair by multi-functional and multi-channel regulation of the protein disorders.展开更多
Objective: To observe the real-time effect of scraping therapy for headache due to common cold. Methods: Forty-five patients with headache caused by cold were included to receive scraping therapy, and asked to take ...Objective: To observe the real-time effect of scraping therapy for headache due to common cold. Methods: Forty-five patients with headache caused by cold were included to receive scraping therapy, and asked to take hot water and a good rest after treatment. Visual Analog Scale (VAS) score was recorded before and after the scraping treatment. The patients were visited again at the second day to inspect the development of the disease, including whether the headache was relapsed or aggravated; how long it was between the relapse or aggravation and the scraping treatment; patients' behavior after the scraping treatment. Result: The VAS score ofpre-treatment was 6.45±1.95, and it was 1.43±0.65 after treatment. The results had statistical significance. In the revisit among the 45 patients, there were 39 patients whose headache didn't relapsed or aggravated. For the left 6 patients who got relapsed or aggravated headache, researcher found that they did not follow the doctor's order well. Conclusion: Scraping therapy has obvious real-time effect on headache caused by cold. However, whether the patients can follow the doctor's advice is a key factor in the relapse of headache.展开更多
文摘Thirty Wistar rats were randomly and evenly divided into control group, cerebral ischemia group and ischemia + electroacupuncture (EA) group. The bilateral common carotid arteries were occluded to induce acute cerebral ischemia. Nitric oxide (NO) and endothelin (ET)contents in the cerebral tissues and blood were measured under normal condition, immediately after ischemia and following EA. Results showed that after acute cerebral ischemia NO and ET contents in the cerebral tissues increased significantly (P<0.01) while serum ET increased and serum NO lowered obviously (P<0.05). Following EA of Baihui (GV 20) and Dazhui (GV 14), both NO and ET in cerebral tissues and serum turned to normal basically. It showed that EA could protect the cerebral tissues from injury induced by ischemia, NO and ET might participate in the modulation process of EA.
文摘Summary : To observe the therapeutic effect of acupuncture plus medicament in the treatment of prostatic hyperplasia, Zhibian (BL 54) and Zhongji (CV 3) were deeply punctured with elongated needles and enema was applied with self-prepared medicinal herbs. Then Qugu (CV2), Zhongliao(BL 33) and Xialiao(BL 34) were selected and connected with a herbal Ion Introduction Apparatus. The total effective rate was 92.4% in 41 cases. Elongated needle and herbal ion introduction can relieve the clinical symptoms of prostatic hyperplasia and improve the patients' life quality.
基金supported by National Natural Science Foundation of China No. 81303051, No. 30901901Province and Ministry Co-construction Key Laboratory for Internal Medicine of Traditional Chinese Medicine of the Education Ministry of China No. ZYNK201501+1 种基金Tuina Department of Yueyang Hostpital Affiliated to Hunan University of Chinese Medicine State Clinical Key SpecialtyTuina Key Discipline of State Administration of Traditional Chinese Medicine~~
文摘Objective: To investigate the effects of acupuncture on the number of associated phosphorylated proteins in brain tissues of middle cerebral artery occlusion (MCAO) rats, based on the protein microarray technique. Methods: The MCAO model was prepared according to the modified occlusion method using occlusion lines. Forty healthy Sprague-Dawley (SD) rats were randomly divided into 4 groups using the lottery method: a sham operation group, a model group, a control point group and an acupoint group, with 10 rats in each group. Rats in the sham operation group and the model group only received binding without acupuncture. Rats in the acupoint group received acupuncture at Dazhui (GV 14), Baihui (GV 20) and Shuigou (GV 25); rats in the control point group received acupuncture at non-acupoint control points. The needle was twisted once for 1 min after insertion and another time in the middle of the 30 min needle retaining. Acupuncture was conducted once every 12 h for 6 consecutive times. At the end of the experiment, the neurological impairment score was collected, and cells of the ischemic brain tissues were extracted. The protein phosphorylation of the related signaling was detected using the 720 phosphorylated antibody microarray technique, and the differentially expressed proteins between groups were screened. Results: The neurological impairment scores after 72 h of treatment: compared with the sham operation group, the scores of the model group, the control point group and the acupoint group were significantly increased (P〈0.01); compared with the model group, the scores of the acupoint group and the control point group were significantly decreased (P〈0.01,P〈0.05); the score of the acupoint group was better than that of the control point group (P〈0.05). The results of the protein microarray: compared with the sham operation group, 48 proteins showed up-regulated phosphorylation (≥1.5 times) in the model group and the down-regulated was 28; compared with the model group, 35 proteins showed up-regulated phosphorylation in the control point group, and the down-regulated was 24. There were 29 proteins showing up-regulated phosphorylation in the acupoint group and the down-regulated was 51. The numbers of proteins involved in the function and signal transduction pathways were also different. Conclusion: Acupuncture at Dazhui (GV 14), Baihui (GV 20) and Shuigou (GV 25) can effectively repair brain injury. The ischemic injury of brain tissue may be caused by imbalance of a variety of proteins, and acupuncture can promote brain tissue repair by multi-functional and multi-channel regulation of the protein disorders.
文摘Objective: To observe the real-time effect of scraping therapy for headache due to common cold. Methods: Forty-five patients with headache caused by cold were included to receive scraping therapy, and asked to take hot water and a good rest after treatment. Visual Analog Scale (VAS) score was recorded before and after the scraping treatment. The patients were visited again at the second day to inspect the development of the disease, including whether the headache was relapsed or aggravated; how long it was between the relapse or aggravation and the scraping treatment; patients' behavior after the scraping treatment. Result: The VAS score ofpre-treatment was 6.45±1.95, and it was 1.43±0.65 after treatment. The results had statistical significance. In the revisit among the 45 patients, there were 39 patients whose headache didn't relapsed or aggravated. For the left 6 patients who got relapsed or aggravated headache, researcher found that they did not follow the doctor's order well. Conclusion: Scraping therapy has obvious real-time effect on headache caused by cold. However, whether the patients can follow the doctor's advice is a key factor in the relapse of headache.