Purpose： To evaluate the effect of a hydroethanolic extract of Moltkia coerulea ointment （MCO） on the healing of excision wound in a rat model. Methods： Circular surgical full thickness excision wound, with 314 mm...Purpose： To evaluate the effect of a hydroethanolic extract of Moltkia coerulea ointment （MCO） on the healing of excision wound in a rat model. Methods： Circular surgical full thickness excision wound, with 314 mm2 size, was induced in the anterior-dorsal side of each rat. Three different doses of MCO （1%, 3% and 6%） were administrated. On Day 3, 7, 14 and 21, the tissue was sampled and immune cells, fibroblasts and fibrocytes distribution per one mm2 of wound area, collagen density and re-epithelialization were analyzed. Moreover, the total flavnoid, phenols and anti-oxidant potential of the MCO were evaluated. Ultimately, the percentage of wound contraction in different groups was compared with each other. Results： Hydroethanolic extract of MCO significantly （p 〈 0.05） increased wound contraction percentage. The animals in medium and high dose MCO-treated groups exhibited remarkably （p 〈 0.05） higher fibroblast and fibrocyte distribution and signifcantly （p 〈 0.05） lower immune cells infiltration. On Day 7 after injury, MCO up-regulated neovascularization in a dose-dependent way. Conclusion： Our data showed that MCO shortened the inflammation phase by provoking the fibroblast proliferation. Moreover, MCO promoted the healing process by up-regulating the angiogenesis and provoking the structural cells proliferation as well as increasing the collagen synthesis, cross-linking, and deposition.展开更多
Objective： The use of fibrin adhesives has a broad background in nerve repair. Currently the suboptimal physical properties of singledonor fibrin adhesives have restricted their usage. The present experiment studies ...Objective： The use of fibrin adhesives has a broad background in nerve repair. Currently the suboptimal physical properties of singledonor fibrin adhesives have restricted their usage. The present experiment studies the performance and physical characteristics of a modified fibrin glue prepared from single-donor human plasma in the repair of posterior tibial nerve of rat. Methods： Forty Wistar rats were divided into 5 groups; in the control group, tibial nerve was completely transected and no treatment was done, while in the four experimental groups the nerve stumps were reconnected by one suture, three sutures, one suture with fibrin glue and fibrin glue alone respectively. During 8 weeks of follow-up, Tibial Function Index was measured weekly and adhesive strength, inflammation and scar formation were assessed at the end of the study. Results： Nerve stumps dehiscence rate and adhesive strength were similar in all experimental groups and significantly differed from control group （P〈0.05）. By the end of the eighth follow-up week, functional recovery of one and three sutures groups were significantly higher than groups in which fibrin glue was used for repair （P〈0.05）. The amount of inflammation and scar tissue formation was similar among all groups. Conclusion： The study results show that the prepared single-donor fibrin adhesive has acceptable mechanical properties which could provide required adhesiveness and hold nerve stumps in the long term; yet, we acknowledge that more studies are needed to improve functional outcome ofsinszle donor fibrin adhesive repair.展开更多
文摘Purpose： To evaluate the effect of a hydroethanolic extract of Moltkia coerulea ointment （MCO） on the healing of excision wound in a rat model. Methods： Circular surgical full thickness excision wound, with 314 mm2 size, was induced in the anterior-dorsal side of each rat. Three different doses of MCO （1%, 3% and 6%） were administrated. On Day 3, 7, 14 and 21, the tissue was sampled and immune cells, fibroblasts and fibrocytes distribution per one mm2 of wound area, collagen density and re-epithelialization were analyzed. Moreover, the total flavnoid, phenols and anti-oxidant potential of the MCO were evaluated. Ultimately, the percentage of wound contraction in different groups was compared with each other. Results： Hydroethanolic extract of MCO significantly （p 〈 0.05） increased wound contraction percentage. The animals in medium and high dose MCO-treated groups exhibited remarkably （p 〈 0.05） higher fibroblast and fibrocyte distribution and signifcantly （p 〈 0.05） lower immune cells infiltration. On Day 7 after injury, MCO up-regulated neovascularization in a dose-dependent way. Conclusion： Our data showed that MCO shortened the inflammation phase by provoking the fibroblast proliferation. Moreover, MCO promoted the healing process by up-regulating the angiogenesis and provoking the structural cells proliferation as well as increasing the collagen synthesis, cross-linking, and deposition.
文摘Objective： The use of fibrin adhesives has a broad background in nerve repair. Currently the suboptimal physical properties of singledonor fibrin adhesives have restricted their usage. The present experiment studies the performance and physical characteristics of a modified fibrin glue prepared from single-donor human plasma in the repair of posterior tibial nerve of rat. Methods： Forty Wistar rats were divided into 5 groups; in the control group, tibial nerve was completely transected and no treatment was done, while in the four experimental groups the nerve stumps were reconnected by one suture, three sutures, one suture with fibrin glue and fibrin glue alone respectively. During 8 weeks of follow-up, Tibial Function Index was measured weekly and adhesive strength, inflammation and scar formation were assessed at the end of the study. Results： Nerve stumps dehiscence rate and adhesive strength were similar in all experimental groups and significantly differed from control group （P〈0.05）. By the end of the eighth follow-up week, functional recovery of one and three sutures groups were significantly higher than groups in which fibrin glue was used for repair （P〈0.05）. The amount of inflammation and scar tissue formation was similar among all groups. Conclusion： The study results show that the prepared single-donor fibrin adhesive has acceptable mechanical properties which could provide required adhesiveness and hold nerve stumps in the long term; yet, we acknowledge that more studies are needed to improve functional outcome ofsinszle donor fibrin adhesive repair.
