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酶联免疫吸附分析法测定重组人生长激素中的大肠杆菌肽 被引量:1
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作者 张培培 杨化新 徐康森 《药物分析杂志》 CAS CSCD 北大核心 1995年第6期30-33,共4页
本文应用双抗夹心式ELISA法,测定重组人生长激素及其制剂中宿主蛋白杂质的含量。采用大肠杆菌肽为标准抗原,辣根过氧化物酶标记抗体,以四甲基联苯胺为底物。测定结果表明,该法的线性范围为0.39~25ng/ml;平均回收... 本文应用双抗夹心式ELISA法,测定重组人生长激素及其制剂中宿主蛋白杂质的含量。采用大肠杆菌肽为标准抗原,辣根过氧化物酶标记抗体,以四甲基联苯胺为底物。测定结果表明,该法的线性范围为0.39~25ng/ml;平均回收率为102.6%;最低检出限量为0.39ng/ml。本法具有特异性强、灵敏度高、定量准确等优点。 展开更多
关键词 ELISA 重组人生长激素 大肠杆菌肽
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Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli 被引量:6
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作者 陈欣欣 余果宇 +3 位作者 张彦 张云 申吉泓 李文辉 《Zoological Research》 CAS CSCD 北大核心 2009年第2期171-177,共7页
OH-CATH is a novel cathelicidin identified from king cobra. It showed strong antibacterial activity against various bacteria in the presence of 1% NaCl and no haemolytic activity toward human red blood cells even at a... OH-CATH is a novel cathelicidin identified from king cobra. It showed strong antibacterial activity against various bacteria in the presence of 1% NaCl and no haemolytic activity toward human red blood cells even at a high concentration. OH-CATH might serve as model molecules for the development of antimicrobial drugs. Understanding the action mechanism of OH-CATH and the reason for its selectivity against microbes is very important for this purpose. The bactericidal effect of the king cobra antimicrobial peptide OH-CATH on Gram-negative Escherichia coli (ATCC 25922) is observed by scanning electron microscopy (SEM) and transmitted electron microscopy (TEM). The SEM and TEM results suggested that the bactericidal mechanism of OH-CATH against Escherichia coli happened in three steps. Firstly, OH-CATH attached to the negatively charged bacterial wall by positively charged amino acid residues. In the second step, the accumulated OH-CATH aggregated and damaged the bacteria membrane in a pore-forming manner. In the last step, with the damage of cell permeability, the contents of the cells were released and eventually cells died. 展开更多
关键词 Antimicrobial peptide OH-CATH Escherichia coli Electron microscopy
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