In this study,a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV)E2 glycoprotein was expressed in E.coli to a obtain target protein.This target protein was used as a coating...In this study,a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV)E2 glycoprotein was expressed in E.coli to a obtain target protein.This target protein was used as a coating antigen to establish an indirect ELISA for specifically detecting anti-CSFV antibodies in serum samples from pigs.The P/N cut-off value of this assay was 1.92 by receiver operating characteristic curve(ROC)analysis based on 30 negative sera and 80 positive samples.The test gave 97.5%sensitivity and 96.7%specificity compared with the indirect hemagglutination(IHA)test.The inter-assay and intra-assay coefficients of variation (CVs)for 16 sera were both≤6.8%.No cross-reactivity between the coating antigen and anti-bovine viral diarrhoea virus(BVDV)antibodies was observed.展开更多
In the present study,we aimed to investigate the effects of Polygonum hydropiper aqueous extract(PHE)on the expression levels of inflammatory cytokines and cytochrome P450(CYPs)in mice with E.coli-induced diarrhea.BAL...In the present study,we aimed to investigate the effects of Polygonum hydropiper aqueous extract(PHE)on the expression levels of inflammatory cytokines and cytochrome P450(CYPs)in mice with E.coli-induced diarrhea.BALB/c mice were randomly divided into the control group,model group,enrofloxacin-treated group,and two PHE-treated groups with different doses.The diarrhea model was established by intraperitoneal injection of enteropathogenic E.coli(EPEC)in mice.The enrofloxacin-treated group was given enrofloxacin at 5 mg/kg by intragastric gavage(i.g.)for 11 d.PHE-treated groups were given PHE at 5 g/kg and 10 g/kg by i.g.for 11 d.The histopathological characteristics of the duodenum and liver were observed by HE staining.The levels of inflammatory cytokines and CYPs in the duodenum and liver of mice were determined by ELISA.The m RNA and protein expressions of CYPs were determined by q RT-PCR and Western blotting analysis,respectively.The results showed that PHE could significantly alleviate the injury of the duodenum and liver induced by EPEC infection,reduce the contents and m RNA expressions of inflammatory cytokines,and regulate the m RNA and protein expressions of the major subtypes of CYPs.These findings indicated that PHE had an apparent therapeutic effect on EPEC-induced diarrhea,and its mechanism might be related to inhibition of inflammatory cytokines and regulation of CYPs.展开更多
文摘In this study,a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV)E2 glycoprotein was expressed in E.coli to a obtain target protein.This target protein was used as a coating antigen to establish an indirect ELISA for specifically detecting anti-CSFV antibodies in serum samples from pigs.The P/N cut-off value of this assay was 1.92 by receiver operating characteristic curve(ROC)analysis based on 30 negative sera and 80 positive samples.The test gave 97.5%sensitivity and 96.7%specificity compared with the indirect hemagglutination(IHA)test.The inter-assay and intra-assay coefficients of variation (CVs)for 16 sera were both≤6.8%.No cross-reactivity between the coating antigen and anti-bovine viral diarrhoea virus(BVDV)antibodies was observed.
基金Key Project at Central Government Level:The ability establishment of sustainable use for valuable Chinese medicine resources(Grant No.2060302-2004-03)。
文摘In the present study,we aimed to investigate the effects of Polygonum hydropiper aqueous extract(PHE)on the expression levels of inflammatory cytokines and cytochrome P450(CYPs)in mice with E.coli-induced diarrhea.BALB/c mice were randomly divided into the control group,model group,enrofloxacin-treated group,and two PHE-treated groups with different doses.The diarrhea model was established by intraperitoneal injection of enteropathogenic E.coli(EPEC)in mice.The enrofloxacin-treated group was given enrofloxacin at 5 mg/kg by intragastric gavage(i.g.)for 11 d.PHE-treated groups were given PHE at 5 g/kg and 10 g/kg by i.g.for 11 d.The histopathological characteristics of the duodenum and liver were observed by HE staining.The levels of inflammatory cytokines and CYPs in the duodenum and liver of mice were determined by ELISA.The m RNA and protein expressions of CYPs were determined by q RT-PCR and Western blotting analysis,respectively.The results showed that PHE could significantly alleviate the injury of the duodenum and liver induced by EPEC infection,reduce the contents and m RNA expressions of inflammatory cytokines,and regulate the m RNA and protein expressions of the major subtypes of CYPs.These findings indicated that PHE had an apparent therapeutic effect on EPEC-induced diarrhea,and its mechanism might be related to inhibition of inflammatory cytokines and regulation of CYPs.
