A high-performance liquid chromatography （HPLC） method has been developed for the first time to simultaneously quantify the four active ingredients, namely aloha, baicalein, aloe-emodin and wogonin, in Dangguilonghu...A high-performance liquid chromatography （HPLC） method has been developed for the first time to simultaneously quantify the four active ingredients, namely aloha, baicalein, aloe-emodin and wogonin, in Dangguilonghui tablet. The marker compotmds were separated on the Diamonsil C18 column at a flow rate of 1.0 mL/min with a mobile phase of methanol-acetonitrile-0.3% aqueous phosphoric acid （220： 4： 200, v/v/v） and while the detection wavelength was set at 225 nm. The assay was linear over the range of 1.450 - 29.00 ug/mL （r = 0.9992） for aloin, 0.4050 - 8.100ug/mL （r = 0.9994） for baicalein, 0.1100 - 2.200 ug/mL （r = 0.9997） for aloe-emodin, 0.2160 - 4.320 ug/mL （r = 0.9991） for wogonin, respectively. The average sample recoveries at three concentration levels were 100.7% （RSD = 0.88%）, 101.0% （RSD = 0.89%）, 100.0% （RSD = 1.3%） and 100.1% （RSD = 1.1%） for these constituents, respectively. This method is suitable for the quality control of Dangguilonghui tablet.展开更多
In order to investigate the protective effect of aloe anthraquinones on growth and development of soybean against ultraviolet B radiation stress from the morphological structure and physio-chemical indices. The result...In order to investigate the protective effect of aloe anthraquinones on growth and development of soybean against ultraviolet B radiation stress from the morphological structure and physio-chemical indices. The results showed that, stressed by the enhanced ultraviolet b radiation, the soybean gave a dwarfed plant, shrunken leaf area and decreased photosynthetic pigment, while an ascended MDA content. Spraying aloe anthraquinones effectively relieved the reductions of chloro- phyll content and biomass and decreased the production of MDA under the radia- tion of UV-B. Moreover, under the UV-B radiation, waxy substances on epidermal cells increased remarkably and the stomas showed obvious subsidence, while spraying aloe anthraquinones could maintain the structure and shape of cells similar to that under natural light, and the stomas subsidence as well.展开更多
Objective To observe the effect of aloe-emodin on the expression of proliferating cell nuclear antigen (PCNA) in smooth muscle cells (SMCs) after arterial injury and study the molecular mechanism of inhibition of alo...Objective To observe the effect of aloe-emodin on the expression of proliferating cell nuclear antigen (PCNA) in smooth muscle cells (SMCs) after arterial injury and study the molecular mechanism of inhibition of aloe-emodin on SMC proliferation.Methods Deendothelialization was performed at the abdominal aorta in Japanese white rabbits using a 3F Fogarty arterial embolectomy catheter. 48 hours later, the medium of abdominal aorta was isolated and primary SMCs culture was performed. Cells were synchronized to G0 by serum starvation, then aloe-emodin at a concentration of 20?μg/ml was added to the culture medium containing 10%［v/v］ fetal calf serum. Vehicle was also added to the medium as a control. After 18 hours, the expression of PCNA at the level of mRNA and protein were examined using techniques of RT/PCR, Western blotting and inmmunocytochemistry respectively. Results Compared with the control group, the expression of PCNA mRNA and protein was prominently decreased after addition of aloe-emodin. Conclusion The inhibition of aloe-emodin on SMCs proliferation may be caused by inhibiting the expression of the PCNA gene.展开更多
This paper reports the extraction of aloe-emodin from aloe by microwave-assisted extraction. The effects of various factors, including the solvent, the ratio （mL/g） of the solvent to the sample, microwave irradiatio...This paper reports the extraction of aloe-emodin from aloe by microwave-assisted extraction. The effects of various factors, including the solvent, the ratio （mL/g） of the solvent to the sample, microwave irradiation time and microwave power, were discussed in the experiments. The yield of aloe-emodin was determined by HPLC. The optimized conditions for micro- wave-assisted extraction of aloe-emodin were concluded as follows： the solvent is 80% ethanol （V/V） solution, microwave ir- radiation time is 3 rain and microwave power is 340 W. Additionally, HPLC fingerprint was developed for consistency evalua- tion of aloe. The similarities of 3 aloe samples obtained by microwave-assisted extraction, ultrasound-assisted extraction and Soxhlet extraction were more than 0.9, indicating that 3 aloe samples were consistent. Compared with Soxhlet extraction and ultrasound-assisted extraction, microwave extraction is a rapid method with higher yield and less solvent consumption. Aloe samples treated by microwave-assisted extraction, ultrasound-assisted extraction and Soxhlet extraction were observed using transmission electronic microscopy. The micrographs provide evidence of more breakage of chloroplasts treated by micro- wave-assisted extraction as compared to Soxhlet extraction and ultrasound-assisted extraction.展开更多
文摘A high-performance liquid chromatography （HPLC） method has been developed for the first time to simultaneously quantify the four active ingredients, namely aloha, baicalein, aloe-emodin and wogonin, in Dangguilonghui tablet. The marker compotmds were separated on the Diamonsil C18 column at a flow rate of 1.0 mL/min with a mobile phase of methanol-acetonitrile-0.3% aqueous phosphoric acid （220： 4： 200, v/v/v） and while the detection wavelength was set at 225 nm. The assay was linear over the range of 1.450 - 29.00 ug/mL （r = 0.9992） for aloin, 0.4050 - 8.100ug/mL （r = 0.9994） for baicalein, 0.1100 - 2.200 ug/mL （r = 0.9997） for aloe-emodin, 0.2160 - 4.320 ug/mL （r = 0.9991） for wogonin, respectively. The average sample recoveries at three concentration levels were 100.7% （RSD = 0.88%）, 101.0% （RSD = 0.89%）, 100.0% （RSD = 1.3%） and 100.1% （RSD = 1.1%） for these constituents, respectively. This method is suitable for the quality control of Dangguilonghui tablet.
基金Supported by National Natural Science Foundation of China(31270225)~~
文摘In order to investigate the protective effect of aloe anthraquinones on growth and development of soybean against ultraviolet B radiation stress from the morphological structure and physio-chemical indices. The results showed that, stressed by the enhanced ultraviolet b radiation, the soybean gave a dwarfed plant, shrunken leaf area and decreased photosynthetic pigment, while an ascended MDA content. Spraying aloe anthraquinones effectively relieved the reductions of chloro- phyll content and biomass and decreased the production of MDA under the radia- tion of UV-B. Moreover, under the UV-B radiation, waxy substances on epidermal cells increased remarkably and the stomas showed obvious subsidence, while spraying aloe anthraquinones could maintain the structure and shape of cells similar to that under natural light, and the stomas subsidence as well.
基金theFoundationoftheMinistryofHealth People’sRepublicofChina (No 94 2 2 0 7)
文摘Objective To observe the effect of aloe-emodin on the expression of proliferating cell nuclear antigen (PCNA) in smooth muscle cells (SMCs) after arterial injury and study the molecular mechanism of inhibition of aloe-emodin on SMC proliferation.Methods Deendothelialization was performed at the abdominal aorta in Japanese white rabbits using a 3F Fogarty arterial embolectomy catheter. 48 hours later, the medium of abdominal aorta was isolated and primary SMCs culture was performed. Cells were synchronized to G0 by serum starvation, then aloe-emodin at a concentration of 20?μg/ml was added to the culture medium containing 10%［v/v］ fetal calf serum. Vehicle was also added to the medium as a control. After 18 hours, the expression of PCNA at the level of mRNA and protein were examined using techniques of RT/PCR, Western blotting and inmmunocytochemistry respectively. Results Compared with the control group, the expression of PCNA mRNA and protein was prominently decreased after addition of aloe-emodin. Conclusion The inhibition of aloe-emodin on SMCs proliferation may be caused by inhibiting the expression of the PCNA gene.
基金supported by the National Key Technology R&D Program (2006BAI09B07-02)
文摘This paper reports the extraction of aloe-emodin from aloe by microwave-assisted extraction. The effects of various factors, including the solvent, the ratio （mL/g） of the solvent to the sample, microwave irradiation time and microwave power, were discussed in the experiments. The yield of aloe-emodin was determined by HPLC. The optimized conditions for micro- wave-assisted extraction of aloe-emodin were concluded as follows： the solvent is 80% ethanol （V/V） solution, microwave ir- radiation time is 3 rain and microwave power is 340 W. Additionally, HPLC fingerprint was developed for consistency evalua- tion of aloe. The similarities of 3 aloe samples obtained by microwave-assisted extraction, ultrasound-assisted extraction and Soxhlet extraction were more than 0.9, indicating that 3 aloe samples were consistent. Compared with Soxhlet extraction and ultrasound-assisted extraction, microwave extraction is a rapid method with higher yield and less solvent consumption. Aloe samples treated by microwave-assisted extraction, ultrasound-assisted extraction and Soxhlet extraction were observed using transmission electronic microscopy. The micrographs provide evidence of more breakage of chloroplasts treated by micro- wave-assisted extraction as compared to Soxhlet extraction and ultrasound-assisted extraction.
