The relationship between echolocation frequency (represented by dominant frequency, DF for short) and body size (body mass, forearm length and body length) in 8 species of horseshoe bats ( Rhinolophus cornutus, R. aff...The relationship between echolocation frequency (represented by dominant frequency, DF for short) and body size (body mass, forearm length and body length) in 8 species of horseshoe bats ( Rhinolophus cornutus, R. affinis, R. thomasi, R. rouxi, R. blythii, R. ferrumequinum, R. pearsoni, R. rex ) was examined. The eight species were captured in caves in five regions (Guiyang, Anlong, Xingyi, Anshun and Zhenning) of Guizhou Province in July and August 1999 and June 2000. The species were identified based on the descriptions in Mammals of GuiZhou (Luo et al .)and Key to the Identification of Chiroptera (Wang, unpublished). The bats were captured at the entrance to the caves at about 8 pm (the time when bats usually leave the caves), and were then put into a recording room near the capture locations where they could fly freely. Recordings of their echolocation calls were made bout 2 hours later using an ultra sound detector (U30, Ultra Sound Advice, UK) which recorded the calls of bats that were flying directly at the microphone at a distance of 1 m. Each bat was recorded 5 times and the signals were fed into a portable ultra sound processor (PUSP, Ultra Sound Advice, UK). The duration for recording was 1 1 s with a sampling frequency of 44 1 kHz. By replaying the recorded tapes the best quality recordings (the loudest and clearest with the least background noise) were replayed at 1/10 speed and re recorded using a digital sound recorder (Sony, MD 1, frequency response range: 30～20 000 Hz). The re recorded echolocation signals were analyzed using the sound processing software Cool Edit 2000, developed by the American Syntrillium Software Company. Ultra sound analysis referred to the sound spectrograms (frequency time graph), time domain spectrograms (energy time graph), energy spectrograms (energy frequency graph), and Hanning window to obtain an analytic precision of 256 Hz. The analysis attenuation was 60 dB. The DF, pulse duration and interpulse interval of the echolocation calls were recorded and the duty cycle, which represents the percentage of the pulse duration in the summation of the pulse duration and the interpulse interval, was calculated. The data are presented as +SD . Body size were measured using a vernier caliper and a balance; all measurements are presented as +SD. When flying, all eight species of bats had similar echolocation signals: the pattern of their echolocation calls was (FM ) CF FM (FM means Frequency modulated; CF means Constant frequency) with 1～2 harmonics. Pulse duration was more than 10 ms, the duty cycle was higher than 40%, the dominant frequency mainly concentrated on the CF part from 25 kHz to 120 kHz. The different species did, however, display different FM widths. Pearson (one of analytical methods in the software SPSS 10 0) was adopted to analyze the correlation between dominant frequency and body size. An obvious negative correlation was found between echolocation frequency and body size in horseshoe bats. The correlation coefficient of DF against forearm length was r=-0 714 (P=0 047; df=6) , DF against body mass r= -0 429 (P=0 289; df=6) and DF against body length r=-0 810 (P=0 015, df=6) . The dominant frequency was higher in smaller species. The species order in terms of dominant frequencies (highest to lowest) was: Rhinolophus cornutus>R. thomasi>R. rouxi>R. blythi>R.ferrumequinum>R. pearsoni>R. affinis>R. rex. The order of forearm length from top to bottom was: Rhinolophus pearsomi>R. rex>R. ferrumequinum>R. affinis>R. thomasi>R. rouxi>R. cornutus>R. blythi. The negative correlation between the dominant frequency and body size was not very strong, presumably a reflection of the influence of factors such as ecological competition and morphological and physiological features other than body size on echolocation calls. Divergence in echolocation calls allows bat species of similar body size to avoid competition with each other. The relationship between dominant frequency and body size probably occurs because the wave length of展开更多
Echolocation calls of 10 Chinese rhinolophid species were recorded to investigate the relationship between morphology and echolocation signals. All horseshoe bats use FM-CF-FM calls. Rhinolophus rex calls at 23.7 kHz,...Echolocation calls of 10 Chinese rhinolophid species were recorded to investigate the relationship between morphology and echolocation signals. All horseshoe bats use FM-CF-FM calls. Rhinolophus rex calls at 23.7 kHz, the lowest frequency in this genus. Call frequency was not correlated with body mass (P=0.200, 9 species). Close negative relationships were found between call frequency and ear length (r=-0.942, P<0.001) and also between call frequency and forearm length (r=-0.696, P<0.05). Residual analysis was carried out to remove the influence of other morphological features. After calculating ear length, forearm length residuals were not significantly related to call frequency (r=-0.095, P=0.808). The significance of the correlation between ear length and call frequency was slightly lowered (r=-0.642, P=0.062) after “removing” the influence of forearm length. Ear length, therefore, was a better predictor of call frequency than forearm length [Acta Zoologica Sinica 49(1):128-133,2003].展开更多
基金Supported by NSFC(30470537)NSF of Anhui Education Committee(ZD2008006-1)+2 种基金NSF of Anhui Provincial Laboratory of the Conservation and Exploitation of Biological ResourcesNSF of Anhui Key Laboratory of Biotic Environment and Ecological SecurityThe Open Foundation of the Key Laboratory of Molecular Evolution and Biodiversity of Anhui Normal University
文摘The relationship between echolocation frequency (represented by dominant frequency, DF for short) and body size (body mass, forearm length and body length) in 8 species of horseshoe bats ( Rhinolophus cornutus, R. affinis, R. thomasi, R. rouxi, R. blythii, R. ferrumequinum, R. pearsoni, R. rex ) was examined. The eight species were captured in caves in five regions (Guiyang, Anlong, Xingyi, Anshun and Zhenning) of Guizhou Province in July and August 1999 and June 2000. The species were identified based on the descriptions in Mammals of GuiZhou (Luo et al .)and Key to the Identification of Chiroptera (Wang, unpublished). The bats were captured at the entrance to the caves at about 8 pm (the time when bats usually leave the caves), and were then put into a recording room near the capture locations where they could fly freely. Recordings of their echolocation calls were made bout 2 hours later using an ultra sound detector (U30, Ultra Sound Advice, UK) which recorded the calls of bats that were flying directly at the microphone at a distance of 1 m. Each bat was recorded 5 times and the signals were fed into a portable ultra sound processor (PUSP, Ultra Sound Advice, UK). The duration for recording was 1 1 s with a sampling frequency of 44 1 kHz. By replaying the recorded tapes the best quality recordings (the loudest and clearest with the least background noise) were replayed at 1/10 speed and re recorded using a digital sound recorder (Sony, MD 1, frequency response range: 30～20 000 Hz). The re recorded echolocation signals were analyzed using the sound processing software Cool Edit 2000, developed by the American Syntrillium Software Company. Ultra sound analysis referred to the sound spectrograms (frequency time graph), time domain spectrograms (energy time graph), energy spectrograms (energy frequency graph), and Hanning window to obtain an analytic precision of 256 Hz. The analysis attenuation was 60 dB. The DF, pulse duration and interpulse interval of the echolocation calls were recorded and the duty cycle, which represents the percentage of the pulse duration in the summation of the pulse duration and the interpulse interval, was calculated. The data are presented as +SD . Body size were measured using a vernier caliper and a balance; all measurements are presented as +SD. When flying, all eight species of bats had similar echolocation signals: the pattern of their echolocation calls was (FM ) CF FM (FM means Frequency modulated; CF means Constant frequency) with 1～2 harmonics. Pulse duration was more than 10 ms, the duty cycle was higher than 40%, the dominant frequency mainly concentrated on the CF part from 25 kHz to 120 kHz. The different species did, however, display different FM widths. Pearson (one of analytical methods in the software SPSS 10 0) was adopted to analyze the correlation between dominant frequency and body size. An obvious negative correlation was found between echolocation frequency and body size in horseshoe bats. The correlation coefficient of DF against forearm length was r=-0 714 (P=0 047; df=6) , DF against body mass r= -0 429 (P=0 289; df=6) and DF against body length r=-0 810 (P=0 015, df=6) . The dominant frequency was higher in smaller species. The species order in terms of dominant frequencies (highest to lowest) was: Rhinolophus cornutus>R. thomasi>R. rouxi>R. blythi>R.ferrumequinum>R. pearsoni>R. affinis>R. rex. The order of forearm length from top to bottom was: Rhinolophus pearsomi>R. rex>R. ferrumequinum>R. affinis>R. thomasi>R. rouxi>R. cornutus>R. blythi. The negative correlation between the dominant frequency and body size was not very strong, presumably a reflection of the influence of factors such as ecological competition and morphological and physiological features other than body size on echolocation calls. Divergence in echolocation calls allows bat species of similar body size to avoid competition with each other. The relationship between dominant frequency and body size probably occurs because the wave length of
文摘Echolocation calls of 10 Chinese rhinolophid species were recorded to investigate the relationship between morphology and echolocation signals. All horseshoe bats use FM-CF-FM calls. Rhinolophus rex calls at 23.7 kHz, the lowest frequency in this genus. Call frequency was not correlated with body mass (P=0.200, 9 species). Close negative relationships were found between call frequency and ear length (r=-0.942, P<0.001) and also between call frequency and forearm length (r=-0.696, P<0.05). Residual analysis was carried out to remove the influence of other morphological features. After calculating ear length, forearm length residuals were not significantly related to call frequency (r=-0.095, P=0.808). The significance of the correlation between ear length and call frequency was slightly lowered (r=-0.642, P=0.062) after “removing” the influence of forearm length. Ear length, therefore, was a better predictor of call frequency than forearm length [Acta Zoologica Sinica 49(1):128-133,2003].
