Background： Exercise-associated menstrual dysfunction （EAMD） is a common health problem in female athletes as a part of female athlete triad （FAT）, a condition related to low energy availability. In this study, w...Background： Exercise-associated menstrual dysfunction （EAMD） is a common health problem in female athletes as a part of female athlete triad （FAT）, a condition related to low energy availability. In this study, we explored the possibility that carbohydrate supplements can improve the status of EAMD and prevent exercise-induced ovarian injury in a FAT rat model. This research aimed to provide experimental evidence with regard to the relationship of energy intervention and EAMD. Methods： Forty-five female Sprague-Dawley rats （2 months old） were randomly divided into five experimental groups： control group （C）, 9-week exercise as model for EAMD （E）, post-EAMD recovery group （R）, oligosaccharide intervention group （O）, and glucose intervention group （G）. All rats were sacrificed at the end of 9 weeks. Serum samples were collected for measuring gonadotropin releasing hormone, follicle stimulating hormone, luteinizing hormone, 1713-estradiol and progesterone levels. The ovaries were taken for investigation of exercise- and carbohydrate-induced follicular subcellular structure changes. Results： Exercise induced irregular menstrual cycles and ovary subcellular structural damages, such as swollenness of mitochondria in rats from groups E and R. Both glucose and oligosaccharide supplements restored well-differentiated mitochondria in the ovarian follicular cells, and a significant improvement of endoplasmic reticulum and Golgi in swollenness in theca cells in groups O and G compared to groups C, E, and R. There was no difference in mitochondria subcellular structural changes between groups O and G. Group E showed attenuation of serum levels of 17β-estradiol and progesterone compared to C. There were no differences of 17β-estradiol serum levels among groups O, G, and R, while group G showed a lower level of progesterone than C. Conclusion： Female adult rats with 9-week continuous exercise can cause menstrual dysregulation as a model for EAMD. Post-EAMD intervention with glucose and oligosaccharide intake can normalize the menstrual cycle, restore the follicular subcellular structure, and reverse the exercise-induced reduction of ovary sex hormones. It suggests a positive feedback of hypothalamus-pituitary-ovary axis might be involved in the molecular mechanisms of energy intake in treating EAMD.展开更多
Objective To explore the effect of heat-reinforcing acupuncture combined with Bobath rehabilita on training for the gross motor func on measurement（GMFM） in children with cerebral palsy. Methods Sixty cases of cereb...Objective To explore the effect of heat-reinforcing acupuncture combined with Bobath rehabilita on training for the gross motor func on measurement（GMFM） in children with cerebral palsy. Methods Sixty cases of cerebral palsy were randomized into a heat-reinforcing acupuncture combined with rehabilitation training group（group A） and a rehabilitation group（group B）,30 cases in each group. Rehabilitation training was applied in group B and heat-reinforcing acupuncture was added in group A. Jiāji（夹脊 EX-B 2）,Jiānyú（肩髃 LI 15）,Qūchí（曲池 LI 11）,Hégi（合谷 LI 4）,Yánglíngquán（阳陵泉 GB 34）,Yīnlíngquán（阴陵泉 SP 9）,Xuánzhōng（悬钟 GB 39）,Zúsānli（足三里 ST 36）,Sānyīnjiāo（三阴交 SP 6）,Chéngshān（承山 BL 57）,Tài chōng（太冲 LR 3）,Tàixī（太溪 KI 3） and Shénmén（神门 HT 4） were chosen in group A and the heat-reinforcing acupuncture was applied once a day. For scalp acupuncture,Biihuì（百会 GV 20）,Sìshéncōng（四神聪 EX-HN 1）,Zhìsānzhēn（智三针）,Nǎosānzhēn（脑三针）,Nièsānzhēn（颞三针） and motor area（运动区） were punctured without any manipulation,once every other day,3 months as a treatment course and 2 courses were needed. GMFM were selected to assess the children with cerebral palsy before treatment,a er 3 months treatment and a er 6 months treatment. Results The total eff ec ve rate in group A was 70.00%（21/30）,which is superior to that of 60.00%（24/30） in group B（P0.05）. In group A,the GMFM scores of decubitus position and turn-over of body,creeping and kneeling,sitting,standing,walking,running and jumping after6 months treatment were significantly improved compared with that before treatment and after 3 months treatment（all P0.05）. In group B,above indices a er 6 months treatment were significantly improved compared with those before treatment,the standing score after 6 months treatment was significantly improved compared with that a er 3 months treatment（P0.05）; the GMFM scores of creeping,kneeling,sitting,standing,walking,running and jumping after 6 months treatment in group A were more significantly improved than that in group B（all P0.05）. Conclusion The heat-reinforcing acupuncture combined with Bobath rehabilita on training has a be er eff ect than rehabilitation training on the GMFM scores of creeping and kneeling,si ng,standing,walking,running and jumping of children with cerebral palsy. It can be shown that the combination of heat-reinforcing acupuncture and Bobath rehabilita on training can improve the gross motor func on of children with cerebral palsy.展开更多
基金supported by Shanghai Key Laboratory of Human Sport Competence Development and Maintenance,Shanghai University of Sport(NO.11DZ2261100)
文摘Background： Exercise-associated menstrual dysfunction （EAMD） is a common health problem in female athletes as a part of female athlete triad （FAT）, a condition related to low energy availability. In this study, we explored the possibility that carbohydrate supplements can improve the status of EAMD and prevent exercise-induced ovarian injury in a FAT rat model. This research aimed to provide experimental evidence with regard to the relationship of energy intervention and EAMD. Methods： Forty-five female Sprague-Dawley rats （2 months old） were randomly divided into five experimental groups： control group （C）, 9-week exercise as model for EAMD （E）, post-EAMD recovery group （R）, oligosaccharide intervention group （O）, and glucose intervention group （G）. All rats were sacrificed at the end of 9 weeks. Serum samples were collected for measuring gonadotropin releasing hormone, follicle stimulating hormone, luteinizing hormone, 1713-estradiol and progesterone levels. The ovaries were taken for investigation of exercise- and carbohydrate-induced follicular subcellular structure changes. Results： Exercise induced irregular menstrual cycles and ovary subcellular structural damages, such as swollenness of mitochondria in rats from groups E and R. Both glucose and oligosaccharide supplements restored well-differentiated mitochondria in the ovarian follicular cells, and a significant improvement of endoplasmic reticulum and Golgi in swollenness in theca cells in groups O and G compared to groups C, E, and R. There was no difference in mitochondria subcellular structural changes between groups O and G. Group E showed attenuation of serum levels of 17β-estradiol and progesterone compared to C. There were no differences of 17β-estradiol serum levels among groups O, G, and R, while group G showed a lower level of progesterone than C. Conclusion： Female adult rats with 9-week continuous exercise can cause menstrual dysregulation as a model for EAMD. Post-EAMD intervention with glucose and oligosaccharide intake can normalize the menstrual cycle, restore the follicular subcellular structure, and reverse the exercise-induced reduction of ovary sex hormones. It suggests a positive feedback of hypothalamus-pituitary-ovary axis might be involved in the molecular mechanisms of energy intake in treating EAMD.
基金Supported by a project from Bureau of Public Health of Shanghai:2008 L 029 A
文摘Objective To explore the effect of heat-reinforcing acupuncture combined with Bobath rehabilita on training for the gross motor func on measurement（GMFM） in children with cerebral palsy. Methods Sixty cases of cerebral palsy were randomized into a heat-reinforcing acupuncture combined with rehabilitation training group（group A） and a rehabilitation group（group B）,30 cases in each group. Rehabilitation training was applied in group B and heat-reinforcing acupuncture was added in group A. Jiāji（夹脊 EX-B 2）,Jiānyú（肩髃 LI 15）,Qūchí（曲池 LI 11）,Hégi（合谷 LI 4）,Yánglíngquán（阳陵泉 GB 34）,Yīnlíngquán（阴陵泉 SP 9）,Xuánzhōng（悬钟 GB 39）,Zúsānli（足三里 ST 36）,Sānyīnjiāo（三阴交 SP 6）,Chéngshān（承山 BL 57）,Tài chōng（太冲 LR 3）,Tàixī（太溪 KI 3） and Shénmén（神门 HT 4） were chosen in group A and the heat-reinforcing acupuncture was applied once a day. For scalp acupuncture,Biihuì（百会 GV 20）,Sìshéncōng（四神聪 EX-HN 1）,Zhìsānzhēn（智三针）,Nǎosānzhēn（脑三针）,Nièsānzhēn（颞三针） and motor area（运动区） were punctured without any manipulation,once every other day,3 months as a treatment course and 2 courses were needed. GMFM were selected to assess the children with cerebral palsy before treatment,a er 3 months treatment and a er 6 months treatment. Results The total eff ec ve rate in group A was 70.00%（21/30）,which is superior to that of 60.00%（24/30） in group B（P0.05）. In group A,the GMFM scores of decubitus position and turn-over of body,creeping and kneeling,sitting,standing,walking,running and jumping after6 months treatment were significantly improved compared with that before treatment and after 3 months treatment（all P0.05）. In group B,above indices a er 6 months treatment were significantly improved compared with those before treatment,the standing score after 6 months treatment was significantly improved compared with that a er 3 months treatment（P0.05）; the GMFM scores of creeping,kneeling,sitting,standing,walking,running and jumping after 6 months treatment in group A were more significantly improved than that in group B（all P0.05）. Conclusion The heat-reinforcing acupuncture combined with Bobath rehabilita on training has a be er eff ect than rehabilitation training on the GMFM scores of creeping and kneeling,si ng,standing,walking,running and jumping of children with cerebral palsy. It can be shown that the combination of heat-reinforcing acupuncture and Bobath rehabilita on training can improve the gross motor func on of children with cerebral palsy.
