本研究测定我国1900份大豆栽培种(G.max)、半野生型(G.gracilis)、野生种(G.soja)的种皮过氧化物酶活性和根部荧光性基因表型频率。研究表明,这两个性状基因在我国 Soja 亚属三个种中的表型频率分布是不同的。野生种和半野生型具有较高...本研究测定我国1900份大豆栽培种(G.max)、半野生型(G.gracilis)、野生种(G.soja)的种皮过氧化物酶活性和根部荧光性基因表型频率。研究表明,这两个性状基因在我国 Soja 亚属三个种中的表型频率分布是不同的。野生种和半野生型具有较高频率的种皮过氧化物酶高活性和根部非荧光性基因,而栽培种则频率较低。各基因表型频率在栽培种中与种皮颜色、生育习性、结荚习性、百粒重等农艺性状有密切关系,其频率分布在我国地理纬度上表现出一定的规律性。结果还表明种皮过氧化物酶低活性基因和荧光性基因可能在大豆栽培驯化过程中得到积累。展开更多
A study was conducted in the field of the Institute of Vegetable Crops, Jiangsu province from July 2000 to August 2003. The transgenic roundup-ready soybean was sown in the middle of the field in a circular manner for...A study was conducted in the field of the Institute of Vegetable Crops, Jiangsu province from July 2000 to August 2003. The transgenic roundup-ready soybean was sown in the middle of the field in a circular manner for 5 circles, with the distance of 3 m, from one circle to another. Then the wild soybean was planted in plots as the rays of the circles in 8 directions (N, E, W, S, NE, NW, SE and SW), spaced every 5 m until 50 m. Each plot comprised 25 plants. In the second year, the wild soybean seeds from the first year were planted in the field together with the original wild soybean as check. Before flowering time, high concentrations of roundups (about 4-5 times of the normal dose) were sprayed on the plants and the surviving plants were identified. The leaves were taken to the lab for DNA extraction to determine the unique DNA for roundup-ready soybean (CTAB method). About 2% of the plants survived, but some leaves were yellow. One plant of wild soybean was found to have the roundup-ready gene from the original roundup-ready soybean. The other surviving wild soybeans should also had some fragments of the roundup tolerance gene. However, the DNA bands were not very clear in the PCR map.展开更多
文摘本研究测定我国1900份大豆栽培种(G.max)、半野生型(G.gracilis)、野生种(G.soja)的种皮过氧化物酶活性和根部荧光性基因表型频率。研究表明,这两个性状基因在我国 Soja 亚属三个种中的表型频率分布是不同的。野生种和半野生型具有较高频率的种皮过氧化物酶高活性和根部非荧光性基因,而栽培种则频率较低。各基因表型频率在栽培种中与种皮颜色、生育习性、结荚习性、百粒重等农艺性状有密切关系,其频率分布在我国地理纬度上表现出一定的规律性。结果还表明种皮过氧化物酶低活性基因和荧光性基因可能在大豆栽培驯化过程中得到积累。
文摘A study was conducted in the field of the Institute of Vegetable Crops, Jiangsu province from July 2000 to August 2003. The transgenic roundup-ready soybean was sown in the middle of the field in a circular manner for 5 circles, with the distance of 3 m, from one circle to another. Then the wild soybean was planted in plots as the rays of the circles in 8 directions (N, E, W, S, NE, NW, SE and SW), spaced every 5 m until 50 m. Each plot comprised 25 plants. In the second year, the wild soybean seeds from the first year were planted in the field together with the original wild soybean as check. Before flowering time, high concentrations of roundups (about 4-5 times of the normal dose) were sprayed on the plants and the surviving plants were identified. The leaves were taken to the lab for DNA extraction to determine the unique DNA for roundup-ready soybean (CTAB method). About 2% of the plants survived, but some leaves were yellow. One plant of wild soybean was found to have the roundup-ready gene from the original roundup-ready soybean. The other surviving wild soybeans should also had some fragments of the roundup tolerance gene. However, the DNA bands were not very clear in the PCR map.
