响应面法对大豆花粉离体萌发主要影响因子的优化

以辽宁省审定的大豆品种辽豆15号花粉为材料,探讨大豆花粉在离体培养条件下萌发、生长的主要影响因子。采用单因素试验筛选出大豆花粉萌发培养基最佳碳源为蔗糖,Ca^(2+)源为CaCl_2,有效植物生长调节剂为GA_3。通过Plackett-Burman设计法对影响大豆花粉管生长的8个因素进行了评价,筛选出具有显著效应的3个因素:初始pH值、蔗糖、GA_3。用最陡爬坡路径逼近花粉管长度响应区域,通过响应面法确定主要影响因素的最佳水平组合。结果表明,大豆花粉离体萌发的各影响因子的最佳水平组合为蔗糖浓度19.2%、GA_3 68.9 mg/L、初始pH值6.47、H_3BO_40.015%、Ca Cl20.05%、PEG-4000 7.5%、温度25℃、时间3 h。优化后离体培养花粉管长度达391.95μm,比优化前提高32.68%。

不同处理方式对蜜蜂采集大豆花粉的影响

为增加蜜蜂访问大豆花的积极性,提高杂交大豆的结荚率,利用巢内悬挂幼虫信息素和饲喂8-Br-c GMP、豆花糖浆处理蜂群,对照组为饲喂糖浆(1:1)的蜂群。采用收集花粉、摄像观察和荧光定量的方法,对3种处理蜂群的花粉重量、采集蜂数量和Amfor和Ammvl的m RNA相对表达量进行比较。采用浓度梯度筛选出的400 LEqu幼虫信息素和0.5 mg/L 8-Br-c GMP、豆花糖浆处理蜂群,对照组大豆花粉重量百分比为3.05%,3个处理组分别为10.42%、8.53%和5.99%,400 LEqu幼虫信息素、0.5 mg/L 8-Brc GMP与对照组之间存在显著差异(P<0.05)。采集大豆花粉的蜜蜂数量百分比,对照组为55.09%,3个处理组均高于对照组,处理组之间均不存在显著差异(P>0.05)。3种处理方式均可以提高蜜蜂采集大豆花粉的积极性,提高采粉蜂的数量,增加访问大豆花的机率。

应用Plackett-Burman设计法筛选大豆花粉萌发的主要影响因素

通过单因素试验筛选出大豆花粉萌发培养基最佳碳源为蔗糖,Ca2+源为CaCl2,有效植物生长调节剂为GA3;采用Plackett-Burman设计法对选取的8个相关影响因素进行了评价,筛选出3个主要影响因素,分别为初始pH、蔗糖和GA3,且3个因素在PB设计的水平范围内对响应值的影响均为正效应。

High correlation of specific IgE sensitization between birch pollen, soy and apple allergens indicates pollen-food allergy syndrome among birch pollen allergic patients in northern China

Background： Birch pollen sensitization and associated pollen-food syndrome among Chinese allergic patients have not been investigated. Methods： Sera from 203 allergic patients from the northern part of China and collected during February to July 2014 were investigated. Specific immunoglobulin E （IgE） against birch pollen extract Bet v and major birch pollen allergen Bet v 1 were measured using the ADVIA Centaur. The presence of major apple allergen Mal d 1 and soy bean allergen Gly m 4 specific IgE was measured by ImmunoCAP 100. Results： Among the 203 sere, 34 sera （16.7%） had specific IgE to Bet v and of these, 28 sere （82.4%） contained Bet v 1-specific IgE. Among the 28 sera with Bet v 1-specific IgE, 27 sera （96.4%） contained Mal d 1-specific IgE and 22 sera （78.6%） contained Gly m 4-specific igE. Of the 34 Bet v-positive sera, 6 sera （17.6%） contained no specific IgE for Bet v 1, Mal d 1, or Gly m 4. Almost all Bet v-positive sera were donated during the birch pollen season. Conclusions： The prevalence of birch allergy among patients visiting health care during pollen season can be as high as 16.7% in Tangshan City. The majority of Chinese birch allergic patients are IgE-sensitized to the major birch pollen allergen Bet v 1 as well as to the major apple allergen Mal d 1 and soy bean allergen Gly m 4. A relatively high number of patients （17.6%） are IgE-sensitized to birch pollen allergen（s） other than Bet v 1. The high prevalence of specific IgE to Mal d 1 and Gly m 4 among Bet v 1-sensitized patients indicates that pollen-food allergy syndrome could be of clinical relevance in China.