The universal primer of Luteovirus was designed and synthesized.An experimental system of RT PCR RFLP was developed in barley yellow dwarf virus (BYDVs).BYDVs can be distinguished qualitatively by RT PCR method.It was...The universal primer of Luteovirus was designed and synthesized.An experimental system of RT PCR RFLP was developed in barley yellow dwarf virus (BYDVs).BYDVs can be distinguished qualitatively by RT PCR method.It was seen that different serotypes of BYDVs have critical different RFLP patters when the PCR producs were digested by restriction enzyme HinfI.The RFLP patterns of 7 isolates of PAV serotype were greatly different.These results indicate there existed sequence variations among different serotypes of BYDVs and vector phenotypes of PAV serotype.There is no difference between MAV serotypes in RFLP analysis.The slight distinction in the segment of coat protein gene of BYDVs can be revealed by RT PCR RFLP system.展开更多
The IPTG inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E.coli BL21(DE3).High level expression of the specific protein was achieved by IPTG induction.The res...The IPTG inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E.coli BL21(DE3).High level expression of the specific protein was achieved by IPTG induction.The results of SDS PAGE and Western blottong show that the expression product which accumulates 19.5% of the total cellular proteins estimated by scanning is 24 kD BYDV GAV coat protein plus eleven amino acids of pET 5a.展开更多
文摘The universal primer of Luteovirus was designed and synthesized.An experimental system of RT PCR RFLP was developed in barley yellow dwarf virus (BYDVs).BYDVs can be distinguished qualitatively by RT PCR method.It was seen that different serotypes of BYDVs have critical different RFLP patters when the PCR producs were digested by restriction enzyme HinfI.The RFLP patterns of 7 isolates of PAV serotype were greatly different.These results indicate there existed sequence variations among different serotypes of BYDVs and vector phenotypes of PAV serotype.There is no difference between MAV serotypes in RFLP analysis.The slight distinction in the segment of coat protein gene of BYDVs can be revealed by RT PCR RFLP system.
文摘The IPTG inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E.coli BL21(DE3).High level expression of the specific protein was achieved by IPTG induction.The results of SDS PAGE and Western blottong show that the expression product which accumulates 19.5% of the total cellular proteins estimated by scanning is 24 kD BYDV GAV coat protein plus eleven amino acids of pET 5a.
