5-FU结合大黄■虫汤诱导胃癌细胞凋亡的实验研究

目的:探讨5-FU结合大黄虫汤诱导胃癌细胞凋亡的作用。方法:将人胃癌BGC—823细胞裸鼠移植瘤动物模型,随机分为4组(每组12只):5-FU结合大黄虫汤组,大黄虫汤组,5-FU组,生理盐水组。连续用药3周,用药结束后脱颈处死裸鼠,统计各组抑瘤率;流式细胞仪检测移植瘤细胞凋亡率,RT-PCR检测凋亡关键因子Caspase-3表达。结果:5-FU结合大黄虫汤组抑瘤率高于大黄虫汤组、5-FU组(P<0.01);5-FU结合大黄虫汤组胃癌细胞凋亡率明显高于大黄虫汤组、5-FU组(P<0.01,P<0.05),5-FU结合大黄虫汤组Caspase-3水平最高,与大黄虫汤组、5-FU组比较有显著差异(P<0.01,P<0.05)。结论:5-FU结合大黄虫汤能提高胃癌细胞凋亡率,抑制胃癌的增长。

大黄■虫汤治疗外科术后非感染性发热(血瘀证)疗效研究

目的观察大黄■虫汤治疗外科手术患者术后非感染性发热(血瘀证)的临床疗效并探讨其作用机制。方法68例患者采用随机数字表法分为治疗组与对照组各34例。对照组给予西医常规治疗,治疗组在对照组基础上加大黄■虫汤口服治疗。通过比较两组患者发热次数、体温降至正常时间、完全退热时间、中医证候积分进行疗效评价。结果治疗组临床总有效率为94.11%,高于对照组的73.53%(P<0.05)。治疗组发热次数、用药后体温降至正常时间、完全退热时间均短于对照组(均P<0.05)。两组治疗后中医证候积分与治疗前比较均降低(均P<0.05),且治疗组中医证候积分低于对照组(P<0.05)。治疗组治疗后发热、手术局部刺痛、肢体麻木中医证候积分均低于对照组(均P<0.05)。但两组皮下瘀斑中医证候积分比较,差别则不大(P>0.05)。结论大黄■虫汤治疗外科术后非感染性发热(血瘀证)患者疗效确切,可降低患者发热次数,使患者体温尽早降至正常,缩短患者住院时间。

Research Progress on Classical Prescriptions in Treating Mammary Glands Hyperplasia

According to Zhongjing Zhang's academic thought,gynecological diseases mostly result from deficiency,accumulated cold,and qi stagnation,so the treatment of gynecological miscellaneous diseases should take qi and blood as the general principle,and focus on the liver.The pathogenesis of mammary glands hyperplasia(MGH)is liver and kidney deficiency,disharmony of the thoroughfare vessel and the conception vessel,the causes of which are emotional imbalance,liver qi depression,and failure of transportation due to spleen deficiency.The superficial causes include qi stagnation,phlegm coagulation,and blood stasis,which are mainly related to the liver,spleen,kidney,the thoroughfare vessel,and the conception vessel.In clinical treatment of MGH,Xiaochaihu Decoction or Sini Powder can be used for qi stagnation syndrome,Xiao Xianxiong Decoction for phlegm coagulation syndrome,Guizhi Fuling Pill or Dahuang Zhechong Pill for spleen deficiency syndrome,and Wenjing Decoction for the deficiency-cold syndrome.