Aim To study the effect of Isorhapontigenin (Iso) on copper-mediatedperoxidation of human low-density lipoprotein (LDL) and on the toxicity of oxidized LDL (ox-LDL) tomouse macrophages in vitro. Methods Human LDL from...Aim To study the effect of Isorhapontigenin (Iso) on copper-mediatedperoxidation of human low-density lipoprotein (LDL) and on the toxicity of oxidized LDL (ox-LDL) tomouse macrophages in vitro. Methods Human LDL from sera df normal lipidemic donors was separated bysequential ultracentrifugation. The separated human IDL 1 mg·mL^(-1) in phosphate buffer saline, pH7.4, was incubated with cupric sulfate (10 μmol·L^(-1) ) at 37℃ for 10 h in the presence orabsence of various concentrations of Iso. Malondialdehyde (MDA) formation, vitamin E consumption,electrophoretic mobility of LDL, mitochondria] membrane potential of mouse peritoneal macrophages,phagocytosis of neutral red, and release of nitric oxide (NO) from macrophages were determined byvarious methods. Results Iso 1 - 100 μmol·L^(-1) significantly inhibited the increase of MDAformation, vitamin E consumption and electrophoretic mobility of LDL induced by Cu^(2+) in aconcentration-dependent manner. The injury of the mitochondrial membrane potential of mouseperitoneal macrophages due to incubation with ox-LDL (0.1 mg·mL^(-1)) at 37℃ for 12 h was markedlyprotected by 10 μmol·L^(-1) Iso. After pretreat-ment of the macrophages with 10 μmol · L^(-1)of Iso and then exposure to ox-LDL for 4 h, the reduction of phagocytosis of neutral red and releaseof NO in response to lipopolysaccharide (IPS) stimulation were significantly prevented. ConclusionIso has protective action against Cu^(2+) - mediated LDL peroxidation and ox-LDL induced toxicity tomacrophages in vitro.展开更多
Objective： To investigate the therapeutic effects and mechanisms of Salvia miltiorrhizae （Danshen） in the treatment of severe acute pancreatitis （SAP）- or obstructive jaundice （OJ）-induced heart injury. Methods...Objective： To investigate the therapeutic effects and mechanisms of Salvia miltiorrhizae （Danshen） in the treatment of severe acute pancreatitis （SAP）- or obstructive jaundice （OJ）-induced heart injury. Methods： A total of 288 rats were used for SAP- （n= 108） and O J-associated （n= 180） experiments. The rats were randomly divided into sham-operated, model control, and Salvia miltiorrhizae-treated groups. According to the difference of time points after operation, SAP rats in each group were subdivided into 3, 6 and 12 h subgroups （n=12）, whereas OJ rats were subdivided into 7, 14, 21, and 28 d subgroups （n=lS）. At the corresponding time points after operation, the mortality rates of the rats, the contents of endotoxin and phospholipase A2 （PLA：） in blood, and pathological changes of the hearts were investigated. Results： The numbers of dead SAP and OJ rats in the treated groups declined as compared with those in the model control group, but not significantly （P〉0.05）. The contents ofendotoxin （at 6 and 12 h in SAP rats and on 7, 14, 21, and 28 d in OJ rats, respectively） and PLA2 （at 6 and 12 h in SAP rats and on 28 d in OJ rats, respectively） in the treated group were significantly lower than those in the model control group （P〈0.01 and P〈0.001, respectively）. Besides, myocardial pathological injuries were mitigated in SAP and OJ rats. Conclusion： In this study, we found that Salvia rniltiorrhizae improved myocardial pathological changes, reduced the content of PLA2 in blood, and decreased the mortality rates of SAP and OJ rats, exerting protective effects on the hearts of the rats.展开更多
文摘Aim To study the effect of Isorhapontigenin (Iso) on copper-mediatedperoxidation of human low-density lipoprotein (LDL) and on the toxicity of oxidized LDL (ox-LDL) tomouse macrophages in vitro. Methods Human LDL from sera df normal lipidemic donors was separated bysequential ultracentrifugation. The separated human IDL 1 mg·mL^(-1) in phosphate buffer saline, pH7.4, was incubated with cupric sulfate (10 μmol·L^(-1) ) at 37℃ for 10 h in the presence orabsence of various concentrations of Iso. Malondialdehyde (MDA) formation, vitamin E consumption,electrophoretic mobility of LDL, mitochondria] membrane potential of mouse peritoneal macrophages,phagocytosis of neutral red, and release of nitric oxide (NO) from macrophages were determined byvarious methods. Results Iso 1 - 100 μmol·L^(-1) significantly inhibited the increase of MDAformation, vitamin E consumption and electrophoretic mobility of LDL induced by Cu^(2+) in aconcentration-dependent manner. The injury of the mitochondrial membrane potential of mouseperitoneal macrophages due to incubation with ox-LDL (0.1 mg·mL^(-1)) at 37℃ for 12 h was markedlyprotected by 10 μmol·L^(-1) Iso. After pretreat-ment of the macrophages with 10 μmol · L^(-1)of Iso and then exposure to ox-LDL for 4 h, the reduction of phagocytosis of neutral red and releaseof NO in response to lipopolysaccharide (IPS) stimulation were significantly prevented. ConclusionIso has protective action against Cu^(2+) - mediated LDL peroxidation and ox-LDL induced toxicity tomacrophages in vitro.
基金supported by the Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang Province (Nos. 2003C130 and 2004C142)the Grave Foundation Project for Technological and Development of Hangzhou City (No. 2003123B19)the Intensive Foundation Project for Technology of Hangzhou City (No. 2004Z006), China
文摘Objective： To investigate the therapeutic effects and mechanisms of Salvia miltiorrhizae （Danshen） in the treatment of severe acute pancreatitis （SAP）- or obstructive jaundice （OJ）-induced heart injury. Methods： A total of 288 rats were used for SAP- （n= 108） and O J-associated （n= 180） experiments. The rats were randomly divided into sham-operated, model control, and Salvia miltiorrhizae-treated groups. According to the difference of time points after operation, SAP rats in each group were subdivided into 3, 6 and 12 h subgroups （n=12）, whereas OJ rats were subdivided into 7, 14, 21, and 28 d subgroups （n=lS）. At the corresponding time points after operation, the mortality rates of the rats, the contents of endotoxin and phospholipase A2 （PLA：） in blood, and pathological changes of the hearts were investigated. Results： The numbers of dead SAP and OJ rats in the treated groups declined as compared with those in the model control group, but not significantly （P〉0.05）. The contents ofendotoxin （at 6 and 12 h in SAP rats and on 7, 14, 21, and 28 d in OJ rats, respectively） and PLA2 （at 6 and 12 h in SAP rats and on 28 d in OJ rats, respectively） in the treated group were significantly lower than those in the model control group （P〈0.01 and P〈0.001, respectively）. Besides, myocardial pathological injuries were mitigated in SAP and OJ rats. Conclusion： In this study, we found that Salvia rniltiorrhizae improved myocardial pathological changes, reduced the content of PLA2 in blood, and decreased the mortality rates of SAP and OJ rats, exerting protective effects on the hearts of the rats.
