The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a...The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a single injection of STZ （streptozotocin） （60 mg/kg bw （body weight））. Twenty diabetic rats, weighing 263 ± 5 g, were divided into two groups fed a casein diet supplemented or not with Po extract （1 g/kg bw）, for four weeks. Control group （n = 6） received 0.23-0.25 mL of citrate buffer and was fed a standard diet during the experiment. The study was carried out at Oran University, Algeria and the entire experiments lasted from September 2011 to July 2012. Blood was obtained from the abdominal aorta of rats after fasting overnight and standard methods were used for the extraction of spices, determination of glycemia, insulinemia, lipid peroxidation and antioxidant enzymes activities. Portulaca oleracea treated compared to untreated rats, glycemia and HbAIc values were respectively 2.8- and 1.7-fold lower. TBARS （thiobarbituric acid reactive substances） concentrations were reduced in RBC （red blood cells） （-54%） and plasma （-65%）. Moreover, in liver and kidney, TBARS values were respectively 1.8- and 2-fold lower. SOD （superoxide dismutase） and GSH-Px （glutathione peroxidase） activities were increased respectively by ＋38% and ＋85%, in liver. GSSG-Red （glutathione reductase） activity was 1.9-fold higher in kidney, while CAT （catalase） was improved in kidney （＋48%）. In RBCs, SOD, GSH-Px, GSSH-Red and CAT activities were increased by 31%, 42%, 56% and ＋50%, respectively. These data have cast a new light on the actions of Portulaca oleracea and its antioxidant potential benefits in preventing diabetes and its complications.展开更多
Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity,chemopreventative and insecticidal properties. In this study,the toxic effects of L. elliptica essential oi...Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity,chemopreventative and insecticidal properties. In this study,the toxic effects of L. elliptica essential oil against Sprague-Dawley rat’s red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125,250,and 500 mg/(kg body weight),respectively,and the control group received distilled water. Full blood count,RBC osmotic fragility,RBC morphological changes,and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb),mean cell hemoglobin concentration (MCHC),mean cell volume (MCV),and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05),the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinocyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However,the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.展开更多
文摘The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a single injection of STZ （streptozotocin） （60 mg/kg bw （body weight））. Twenty diabetic rats, weighing 263 ± 5 g, were divided into two groups fed a casein diet supplemented or not with Po extract （1 g/kg bw）, for four weeks. Control group （n = 6） received 0.23-0.25 mL of citrate buffer and was fed a standard diet during the experiment. The study was carried out at Oran University, Algeria and the entire experiments lasted from September 2011 to July 2012. Blood was obtained from the abdominal aorta of rats after fasting overnight and standard methods were used for the extraction of spices, determination of glycemia, insulinemia, lipid peroxidation and antioxidant enzymes activities. Portulaca oleracea treated compared to untreated rats, glycemia and HbAIc values were respectively 2.8- and 1.7-fold lower. TBARS （thiobarbituric acid reactive substances） concentrations were reduced in RBC （red blood cells） （-54%） and plasma （-65%）. Moreover, in liver and kidney, TBARS values were respectively 1.8- and 2-fold lower. SOD （superoxide dismutase） and GSH-Px （glutathione peroxidase） activities were increased respectively by ＋38% and ＋85%, in liver. GSSG-Red （glutathione reductase） activity was 1.9-fold higher in kidney, while CAT （catalase） was improved in kidney （＋48%）. In RBCs, SOD, GSH-Px, GSSH-Red and CAT activities were increased by 31%, 42%, 56% and ＋50%, respectively. These data have cast a new light on the actions of Portulaca oleracea and its antioxidant potential benefits in preventing diabetes and its complications.
基金Project (No. 02-01-02-SF0205) supported by the Ministry of Science, Technology and Innovation of Malaysia
文摘Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity,chemopreventative and insecticidal properties. In this study,the toxic effects of L. elliptica essential oil against Sprague-Dawley rat’s red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125,250,and 500 mg/(kg body weight),respectively,and the control group received distilled water. Full blood count,RBC osmotic fragility,RBC morphological changes,and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb),mean cell hemoglobin concentration (MCHC),mean cell volume (MCV),and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05),the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinocyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However,the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.
