目的探究安宫牛黄丸对脑梗塞大鼠血清血管内皮生长因子水平的影响。方法选取60只健康大鼠作为研究对象,随机分为治疗组和对照组。使用Longa线栓法制备局灶脑缺血模型,对照组大鼠采用3 m L生理盐水灌胃;治疗组以等量安宫牛黄丸生理盐水...目的探究安宫牛黄丸对脑梗塞大鼠血清血管内皮生长因子水平的影响。方法选取60只健康大鼠作为研究对象,随机分为治疗组和对照组。使用Longa线栓法制备局灶脑缺血模型,对照组大鼠采用3 m L生理盐水灌胃;治疗组以等量安宫牛黄丸生理盐水溶液灌胃(1.13 g/54 m L)。灌胃14 d后,检测两组大鼠脑神经缺损评分及血清VEGF水平。结果治疗后治疗组大鼠血清VEGF水平高于对照组,脑神经缺损评分显著低于对照组(P<0.05)。结论安宫牛黄丸能够提升局灶脑缺血大鼠血清VEGF水平,对脑梗塞大鼠有治疗效果。展开更多
Objective： To observe the effect of electroacupuncture （EA） on the expression of erythropoie-tin-producing hepatocyte receptor B2 （EphB2） in the cortex around the infracted area of middle cerebral artery occlusi...Objective： To observe the effect of electroacupuncture （EA） on the expression of erythropoie-tin-producing hepatocyte receptor B2 （EphB2） in the cortex around the infracted area of middle cerebral artery occlusion （MCAO） rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia. Methods： A total of 180 male Sprague-Dawley （SD） rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups： postoperative 3 d, postoperative 14 d and postoperative 2l d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride （TFC） staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators. Results： The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group （P〈0.05）. The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells （EphB2＋） around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group （P〈0.05）, and the positive cell number was higher than that in the sham operation group （P〈0.01）. Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2＋ and postsynaptic density-95 positive （PSD-95＋） were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group （P〈0.05）. Conclusion： Electroacupuncture at Ganshu （BL 18） and Shenshu （BL 23） can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.展开更多
文摘目的探究安宫牛黄丸对脑梗塞大鼠血清血管内皮生长因子水平的影响。方法选取60只健康大鼠作为研究对象,随机分为治疗组和对照组。使用Longa线栓法制备局灶脑缺血模型,对照组大鼠采用3 m L生理盐水灌胃;治疗组以等量安宫牛黄丸生理盐水溶液灌胃(1.13 g/54 m L)。灌胃14 d后,检测两组大鼠脑神经缺损评分及血清VEGF水平。结果治疗后治疗组大鼠血清VEGF水平高于对照组,脑神经缺损评分显著低于对照组(P<0.05)。结论安宫牛黄丸能够提升局灶脑缺血大鼠血清VEGF水平,对脑梗塞大鼠有治疗效果。
基金supported by the Open Fund for Colleges and Universities Innovation Platform of Hunan Province,No.14K070Key Project of Hunan Province Administration of Traditional Chinese Medicine,No.201310Innovation Fund Project for Graduate Student of Hunan Province,No.CX2014B360~~
文摘Objective： To observe the effect of electroacupuncture （EA） on the expression of erythropoie-tin-producing hepatocyte receptor B2 （EphB2） in the cortex around the infracted area of middle cerebral artery occlusion （MCAO） rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia. Methods： A total of 180 male Sprague-Dawley （SD） rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups： postoperative 3 d, postoperative 14 d and postoperative 2l d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride （TFC） staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators. Results： The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group （P〈0.05）. The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells （EphB2＋） around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group （P〈0.05）, and the positive cell number was higher than that in the sham operation group （P〈0.01）. Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2＋ and postsynaptic density-95 positive （PSD-95＋） were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group （P〈0.05）. Conclusion： Electroacupuncture at Ganshu （BL 18） and Shenshu （BL 23） can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.
