The aim of this work was to study the effect of condensed tannins （CT） on the kinetic of gas production in vitro, on the three main species of Azorean pasture： Trifolium repens, Lotus corniculatus and Lolium perenn...The aim of this work was to study the effect of condensed tannins （CT） on the kinetic of gas production in vitro, on the three main species of Azorean pasture： Trifolium repens, Lotus corniculatus and Lolium perenne, using Quebracho extract as the source of CT, and also to calculate total tannins and condensed tannins on the species studied. Lotus corniculatus, Lolium perenne and Trifolium repens were tested in vitro with the inclusion of Quebracho extract in doses of 0%, 2.5% and 5% DM. The total content in tannins was determined in the 3 species. We have found, in comparison with standard tannic acid, that tannins exist in the flower of T. repens （0.81 equivalents of tannic acid） and in L .corniculatus （1.07 equivalents of tannic acid） but not in L .perenne （0 equivalents of tannic acid）. The concentration of condensed tannins in the 3 species was： 0.34 mg/mL for T. repens; 0.83 mg/mL for L. corniculatus and 0 mg/ml for L. perenne. We verified that a significant reduction （P 〈 0.05） of gas production happened for doses of 5% of CT in comparison with the other two concentrations： 0% and 2.5% of CT. The results of this study express a reduction of the fermentation rate, which implies a reduction of gas production, so, a reduction of methane emission to the atmosphere and an increase of exploitation of the protein by ruminants.展开更多
This study was conducted to study the effects of a commercial exogenous digestive enzymes （EDE） containing cellulases, xylanases, u-amylase and proteases from anaerobic bacterium and natural enhancer mixture （NEM）...This study was conducted to study the effects of a commercial exogenous digestive enzymes （EDE） containing cellulases, xylanases, u-amylase and proteases from anaerobic bacterium and natural enhancer mixture （NEM） containing allicin, high unit hydrolytic enzyme, Bacillus subtilis spores and ginseng extracts on survival, growth, carcass composition and feed cost/benefit in rabbitfish, Siganus rivulatus. Nine net cages （100 cm × 100 cm × 40 cm; L ×W ×H） were stocked with 10 juvenile rabbitfish （1.1 g per fish） each and placed in a large rectangular tank and offered feed at 4% body weight daily. Three replicates per treatment were offered three isonitrogenous and isocaloric diets, control and two supplemented diets with EDE or NEM at 2 g/kg diet for 74 days. Fish in all cages were weighed at two-week intervals and feed regimen was adjusted accordingly. Rabbitfish offered the control diet exhibited lower growth significantly （P 〈 0.05） and feed utilization than the other two experimental treatments. Experimental diet supplemented with NEM recorded the highest growth performance, feed conversion ratio, protein efficiency ratio, net protein utilization and energy retention significantly （P 〈 0.05） than the other two tested groups. Carcass composition and somatic parameters index was not affected by dietary EDE or NEM inclusion. Ultimately, when all variables are considered, EDE and NEM inclusion to diets appears to reduce feed cost per unit growth of rabbitfish.展开更多
[Objective]As a mosquito-repelling ornamental plant,Pelargonium×Citrosum Vanleenii(P.× Citrosum Vanleenii)is hard to be acquired because of its hybrid background,the paper was to a new regeneration system of...[Objective]As a mosquito-repelling ornamental plant,Pelargonium×Citrosum Vanleenii(P.× Citrosum Vanleenii)is hard to be acquired because of its hybrid background,the paper was to a new regeneration system of(P.× Citrosum Vanleenii).[Method] By studying the influence of plant growth regulators(PGRs)on explant type(leaves and petioles),the optimal combinations of PGRs to maximize SELSs(somatic embryo-like structure)and buds were established.[Result]0.2 mg/L NAA+1.0 mg/L BA was best for LS(leaves segments)and 0.2 mg/L NAA + 1.5 mg/L BAs was best for PS(petioles segments).Cultured plantlets were successfully acclimatized in soil where they grew normally without any morphological variation.Although both LS and PS were usable,the leaf was a better explant for induction of embryogenic calli,somatic embryo-like structures and buds.[Conclusion]This work offered a rapid and efficient system for plant regeneration of P.×Citrosum Vanleenii.展开更多
Hip dislocation in children can occur congenitally in isolation or in conjunction with other con- genital abnormalities. Traumatic hip dislocations in children are relatively uncommon and anterior dislocation of hip j...Hip dislocation in children can occur congenitally in isolation or in conjunction with other con- genital abnormalities. Traumatic hip dislocations in children are relatively uncommon and anterior dislocation of hip joint is even rarer. We report such a case following unusual mode of injury in a 12-year-old child. The patient underwent suc- cessful emergent closed reduction of left hip. The clinicalcourse and follow-up assessment of the patient was other- wise uneventful. At 2 years＇ follow-up there was no evi- dence of osteoarthritis, coxa magna, heterotrophic calcification, in congruency of the joints or avascular ne- crosis of the head of femur.展开更多
Celestine blue(CB)was introduced as a new electroactive indicator in DNA biosensors.The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods.The effect of buffer kind and p H on the...Celestine blue(CB)was introduced as a new electroactive indicator in DNA biosensors.The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods.The effect of buffer kind and p H on the electrochemical behavior of CB was studied.The peak currents of CB were linearly related to DNA concentration in the range of 5.0×10^(-9) to 1.0×10^(-7)mol/L.The detection limit of this approach was 4.76×10^(-10) mol/L.Based on spectrometry data a hypochromic effect was observed in UV-Vis spectra of CB with increasing DNA concentration.The results illustrate the possible interaction mode between CB and DNA is electrostatic binding.展开更多
文摘The aim of this work was to study the effect of condensed tannins （CT） on the kinetic of gas production in vitro, on the three main species of Azorean pasture： Trifolium repens, Lotus corniculatus and Lolium perenne, using Quebracho extract as the source of CT, and also to calculate total tannins and condensed tannins on the species studied. Lotus corniculatus, Lolium perenne and Trifolium repens were tested in vitro with the inclusion of Quebracho extract in doses of 0%, 2.5% and 5% DM. The total content in tannins was determined in the 3 species. We have found, in comparison with standard tannic acid, that tannins exist in the flower of T. repens （0.81 equivalents of tannic acid） and in L .corniculatus （1.07 equivalents of tannic acid） but not in L .perenne （0 equivalents of tannic acid）. The concentration of condensed tannins in the 3 species was： 0.34 mg/mL for T. repens; 0.83 mg/mL for L. corniculatus and 0 mg/ml for L. perenne. We verified that a significant reduction （P 〈 0.05） of gas production happened for doses of 5% of CT in comparison with the other two concentrations： 0% and 2.5% of CT. The results of this study express a reduction of the fermentation rate, which implies a reduction of gas production, so, a reduction of methane emission to the atmosphere and an increase of exploitation of the protein by ruminants.
文摘This study was conducted to study the effects of a commercial exogenous digestive enzymes （EDE） containing cellulases, xylanases, u-amylase and proteases from anaerobic bacterium and natural enhancer mixture （NEM） containing allicin, high unit hydrolytic enzyme, Bacillus subtilis spores and ginseng extracts on survival, growth, carcass composition and feed cost/benefit in rabbitfish, Siganus rivulatus. Nine net cages （100 cm × 100 cm × 40 cm; L ×W ×H） were stocked with 10 juvenile rabbitfish （1.1 g per fish） each and placed in a large rectangular tank and offered feed at 4% body weight daily. Three replicates per treatment were offered three isonitrogenous and isocaloric diets, control and two supplemented diets with EDE or NEM at 2 g/kg diet for 74 days. Fish in all cages were weighed at two-week intervals and feed regimen was adjusted accordingly. Rabbitfish offered the control diet exhibited lower growth significantly （P 〈 0.05） and feed utilization than the other two experimental treatments. Experimental diet supplemented with NEM recorded the highest growth performance, feed conversion ratio, protein efficiency ratio, net protein utilization and energy retention significantly （P 〈 0.05） than the other two tested groups. Carcass composition and somatic parameters index was not affected by dietary EDE or NEM inclusion. Ultimately, when all variables are considered, EDE and NEM inclusion to diets appears to reduce feed cost per unit growth of rabbitfish.
基金Supported by Fund of Jinhua Science Technology Foundation of China(2009-2-02)
文摘[Objective]As a mosquito-repelling ornamental plant,Pelargonium×Citrosum Vanleenii(P.× Citrosum Vanleenii)is hard to be acquired because of its hybrid background,the paper was to a new regeneration system of(P.× Citrosum Vanleenii).[Method] By studying the influence of plant growth regulators(PGRs)on explant type(leaves and petioles),the optimal combinations of PGRs to maximize SELSs(somatic embryo-like structure)and buds were established.[Result]0.2 mg/L NAA+1.0 mg/L BA was best for LS(leaves segments)and 0.2 mg/L NAA + 1.5 mg/L BAs was best for PS(petioles segments).Cultured plantlets were successfully acclimatized in soil where they grew normally without any morphological variation.Although both LS and PS were usable,the leaf was a better explant for induction of embryogenic calli,somatic embryo-like structures and buds.[Conclusion]This work offered a rapid and efficient system for plant regeneration of P.×Citrosum Vanleenii.
文摘Hip dislocation in children can occur congenitally in isolation or in conjunction with other con- genital abnormalities. Traumatic hip dislocations in children are relatively uncommon and anterior dislocation of hip joint is even rarer. We report such a case following unusual mode of injury in a 12-year-old child. The patient underwent suc- cessful emergent closed reduction of left hip. The clinicalcourse and follow-up assessment of the patient was other- wise uneventful. At 2 years＇ follow-up there was no evi- dence of osteoarthritis, coxa magna, heterotrophic calcification, in congruency of the joints or avascular ne- crosis of the head of femur.
文摘Celestine blue(CB)was introduced as a new electroactive indicator in DNA biosensors.The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods.The effect of buffer kind and p H on the electrochemical behavior of CB was studied.The peak currents of CB were linearly related to DNA concentration in the range of 5.0×10^(-9) to 1.0×10^(-7)mol/L.The detection limit of this approach was 4.76×10^(-10) mol/L.Based on spectrometry data a hypochromic effect was observed in UV-Vis spectra of CB with increasing DNA concentration.The results illustrate the possible interaction mode between CB and DNA is electrostatic binding.
