[Objective]The research aimed to study the calling and mating behavior of Malacosoma rectifascia Lajonquère and provide theoretical basis for utilization of sex pheromone to prevent and control M.rectifascia. [Me...[Objective]The research aimed to study the calling and mating behavior of Malacosoma rectifascia Lajonquère and provide theoretical basis for utilization of sex pheromone to prevent and control M.rectifascia. [Method]Through indoor and outdoor observation,effects of M.rectifascia mating behavior occurrence process,calling period,different sex ratio and population density on mating rate were studied. [Result]The sex ratio of M.rectifascia in nature was 1∶1.2,while in lab was 1∶1.8; the calling peak took place from 19：00 to 21：00;different sex ratio and population density had significant effects on mating behavior,and mating rate would be reduced if the population density was higher; both male and female M.rectifascia mate only once throughout their life. [Conclusion]Controlling M.rectifascia with sex pheromone has good application prospects.展开更多
The height growth of bamboo in 2008 and 2009 as well as the water contents of bamboo stump,leaves,straw,and rhizome in 2009 were measured at the Tianmu Mountain Nature Reserve.The results revealed that the relationshi...The height growth of bamboo in 2008 and 2009 as well as the water contents of bamboo stump,leaves,straw,and rhizome in 2009 were measured at the Tianmu Mountain Nature Reserve.The results revealed that the relationship between the height growth（Pn）and growth time（t）showed S-shaped curve in the two years;the water contents of bamboo stump,leaf,straw,rhizome and bamboo shoot were 0.50-2.31,0.46-1.8,0.50-1.38,0.56-1.58 and 0.89-8.96,respectively.Among them,the water content of bamboo shoot was the highest and noticeable changed,followed by bamboo stump,leaves,rhizome and straw in order.The water contents of the underground and aboveground parts respectively were 0.34-0.62 and 0.33-0.57.展开更多
Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleos...Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleosides were determined by using high performance capillary electrophoresis (HPCE). Beckman P/ACE System 5010 apparatus equipped with a UV detector and a Beckman untreated fused-silica capillary (57 cm 75 mm, 50 cm effective length) was used. Before sample injection, the capillary was rinsed with 1 molL-1 sodium hydroxide solution and running buffer for 5 min, respectively. A voltage of 20 kV was applied for the separation. Pressure injection was 586 kPa for 6 seconds, and the wavelength of detector was 254 nm. The running time was 20 min at 20 oC. The effect of humidity and heat on the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia was observed for 1, 3, 5 and 10 days at temperature 40 oC, and relative humidity 75%. Results: The content of nucleosides from natural Cordyceps sinensis was higher than that from cultured Cordyceps mycelia. But the contents of nucleosides from freshly collected natural Cordyceps sinensis were very low, even below the limit of quantitation. The contents of nucleosides from natural Cordyceps sinensis were significantly increased by humidity and heat, but this phenomenon was not observed in cultured Cordyceps mycelia. Conclusion: There are differences between the nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia. The nucleosides in natural Cordyceps sinensis may be derived from the degradation of nucleic acids. This implies that adenosine being used for the quality control of natural Cordyceps sinensis may have to be reconsidered.展开更多
[Objective] The aim was to provide theoretical basis for the cultivation and medicinal exploitation of Gastrodia e/ata. [Method] With four varieties of G. elata （Wu G. elata, Hong G. elata, Lv G. elata, Wu-Hong G. e/...[Objective] The aim was to provide theoretical basis for the cultivation and medicinal exploitation of Gastrodia e/ata. [Method] With four varieties of G. elata （Wu G. elata, Hong G. elata, Lv G. elata, Wu-Hong G. e/ata） as test matedals, box planting method and capillary electrophoresis determination were used to conduct the comparative analysis on the planting effect and biochemical fingerprint of four varieties of G. elata which were planted in the same conditions. [Result] There were no significant differences in the number of juvenile tubers after planting for 5 months, and the variety with the best growth potential was Hong G. elata. At 17 months after cultivation, there were no significant differences in the number of im- mature tubers, but the difference in the weight of immature tubers reached the significant level among the 4 varieties, among which Hong G. elata had the largest weight with the average reaching 3 042.20 g. After planting for 17 months, immature tubers of the 4 varieties were determined using capillary electrophoresis, and 4 large peaks were obtained on the maps, in which the 4th peak and the 1st peak had significant specificity. The 4th peak and 1st peak area ratio of Wu G. elata BI. was greater than or equal to 1, showing no intraspecific variation. [Conclusion] The study on the suitable growth environment and variation occurrence period of G. elata could provide theoretical basis for icultivation of G. elata.展开更多
The optimal formula of Beta-Cypermethrin aqueous capsule suspension was screened out through the experiment, preparation and manufacture of basic formulas, which has no sedimentation, suspended drops of oil, peels and...The optimal formula of Beta-Cypermethrin aqueous capsule suspension was screened out through the experiment, preparation and manufacture of basic formulas, which has no sedimentation, suspended drops of oil, peels and agglomerating and the diameter of capsule is in range of 10-30 靘, conformed to the technical requirement. The aftereffect of the 3.3% Beta-Cypermethrin aqueous capsule suspension which was manufactured according to the optimal formula was up more than 25 days. The experiments on controlling the larvae of Dendrolinmus superans Butler and the adults of Xylotrechus rusticus L were carried out with different concentrations of this chemical. The death rate reached 80% when 250 times solution of the chemical was sprayed on stem to control the larvae of D. superans. For control of the adults of X. rusticus, 200, 400 and 600 times solution of the chemical were applied and their control effects (death rate) reached 85.23%, 74.21% and 66.59% respec-tively. Two kinds of solution (200 times and 300 times) of the chemical were used to control the larvae of D. superans in large area, and the control effect for both concentrations was over 90%.展开更多
基金Supported by Shanxi Agricultural University Technology Innovation Fund Projects (2005011, 2004003)~~
文摘[Objective]The research aimed to study the calling and mating behavior of Malacosoma rectifascia Lajonquère and provide theoretical basis for utilization of sex pheromone to prevent and control M.rectifascia. [Method]Through indoor and outdoor observation,effects of M.rectifascia mating behavior occurrence process,calling period,different sex ratio and population density on mating rate were studied. [Result]The sex ratio of M.rectifascia in nature was 1∶1.2,while in lab was 1∶1.8; the calling peak took place from 19：00 to 21：00;different sex ratio and population density had significant effects on mating behavior,and mating rate would be reduced if the population density was higher; both male and female M.rectifascia mate only once throughout their life. [Conclusion]Controlling M.rectifascia with sex pheromone has good application prospects.
基金Supported by Science and Technology Department 973 Program(2005CB422207,2002CB111504,2002CB410811,2005CB422208)Science and Technology Department Data Share Platform Construction Program(2006DKA32300-08)+2 种基金Science Department National Cooperation Program(200073819)National Natural Science Fund Program(40671132)Scientific Fundamental Work Special Program(2007FY110300-8)~~
文摘The height growth of bamboo in 2008 and 2009 as well as the water contents of bamboo stump,leaves,straw,and rhizome in 2009 were measured at the Tianmu Mountain Nature Reserve.The results revealed that the relationship between the height growth（Pn）and growth time（t）showed S-shaped curve in the two years;the water contents of bamboo stump,leaf,straw,rhizome and bamboo shoot were 0.50-2.31,0.46-1.8,0.50-1.38,0.56-1.58 and 0.89-8.96,respectively.Among them,the water content of bamboo shoot was the highest and noticeable changed,followed by bamboo stump,leaves,rhizome and straw in order.The water contents of the underground and aboveground parts respectively were 0.34-0.62 and 0.33-0.57.
文摘Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleosides were determined by using high performance capillary electrophoresis (HPCE). Beckman P/ACE System 5010 apparatus equipped with a UV detector and a Beckman untreated fused-silica capillary (57 cm 75 mm, 50 cm effective length) was used. Before sample injection, the capillary was rinsed with 1 molL-1 sodium hydroxide solution and running buffer for 5 min, respectively. A voltage of 20 kV was applied for the separation. Pressure injection was 586 kPa for 6 seconds, and the wavelength of detector was 254 nm. The running time was 20 min at 20 oC. The effect of humidity and heat on the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia was observed for 1, 3, 5 and 10 days at temperature 40 oC, and relative humidity 75%. Results: The content of nucleosides from natural Cordyceps sinensis was higher than that from cultured Cordyceps mycelia. But the contents of nucleosides from freshly collected natural Cordyceps sinensis were very low, even below the limit of quantitation. The contents of nucleosides from natural Cordyceps sinensis were significantly increased by humidity and heat, but this phenomenon was not observed in cultured Cordyceps mycelia. Conclusion: There are differences between the nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia. The nucleosides in natural Cordyceps sinensis may be derived from the degradation of nucleic acids. This implies that adenosine being used for the quality control of natural Cordyceps sinensis may have to be reconsidered.
基金Supported by the Fund Project for Scientific Research of the Education Department of Yunnan(2016ZDX150)~~
文摘[Objective] The aim was to provide theoretical basis for the cultivation and medicinal exploitation of Gastrodia e/ata. [Method] With four varieties of G. elata （Wu G. elata, Hong G. elata, Lv G. elata, Wu-Hong G. e/ata） as test matedals, box planting method and capillary electrophoresis determination were used to conduct the comparative analysis on the planting effect and biochemical fingerprint of four varieties of G. elata which were planted in the same conditions. [Result] There were no significant differences in the number of juvenile tubers after planting for 5 months, and the variety with the best growth potential was Hong G. elata. At 17 months after cultivation, there were no significant differences in the number of im- mature tubers, but the difference in the weight of immature tubers reached the significant level among the 4 varieties, among which Hong G. elata had the largest weight with the average reaching 3 042.20 g. After planting for 17 months, immature tubers of the 4 varieties were determined using capillary electrophoresis, and 4 large peaks were obtained on the maps, in which the 4th peak and the 1st peak had significant specificity. The 4th peak and 1st peak area ratio of Wu G. elata BI. was greater than or equal to 1, showing no intraspecific variation. [Conclusion] The study on the suitable growth environment and variation occurrence period of G. elata could provide theoretical basis for icultivation of G. elata.
文摘The optimal formula of Beta-Cypermethrin aqueous capsule suspension was screened out through the experiment, preparation and manufacture of basic formulas, which has no sedimentation, suspended drops of oil, peels and agglomerating and the diameter of capsule is in range of 10-30 靘, conformed to the technical requirement. The aftereffect of the 3.3% Beta-Cypermethrin aqueous capsule suspension which was manufactured according to the optimal formula was up more than 25 days. The experiments on controlling the larvae of Dendrolinmus superans Butler and the adults of Xylotrechus rusticus L were carried out with different concentrations of this chemical. The death rate reached 80% when 250 times solution of the chemical was sprayed on stem to control the larvae of D. superans. For control of the adults of X. rusticus, 200, 400 and 600 times solution of the chemical were applied and their control effects (death rate) reached 85.23%, 74.21% and 66.59% respec-tively. Two kinds of solution (200 times and 300 times) of the chemical were used to control the larvae of D. superans in large area, and the control effect for both concentrations was over 90%.
