Bacillus subtilis produces many chemlcally-dwerse seconaary metaDolltes or interest to chemists ano biologlsts. Based on this, this review gives a detailed overview of the natural components produced by B. subtilis in...Bacillus subtilis produces many chemlcally-dwerse seconaary metaDolltes or interest to chemists ano biologlsts. Based on this, this review gives a detailed overview of the natural components produced by B. subtilis including cyclic lipopeptides, polypeptides, proteins （enzymes）, and non-peptide products. Their structures, bioactive ac- tivities and the relevant variants as novel lead structures for drug discovery are also described. The challenging effects of fermentation metabolites, isolation and purification, as well as the overproduction of bioactive com- pounds from B. subtilis by metabolic engineering, ＇~ere also highlighted. Systematically exploring biosynthetic routes and the functions of secondary metabolites from 13. subtilis may not only be beneficial in improving yields of the products, but also in helping them to be used in food industry and public medical service on a large-scale.展开更多
The Diels–Alder(D–A)reaction is one of the most powerful reactions in organic synthesis.The intermolecular D–A reaction attracts much less attentions than its intramolecular counterpart in natural product synthesis...The Diels–Alder(D–A)reaction is one of the most powerful reactions in organic synthesis.The intermolecular D–A reaction attracts much less attentions than its intramolecular counterpart in natural product synthesis,possibly due to the issues of reactivity and selectivity.In the past decade,the intermolecular D–A reaction has been increasingly utilized in the total synthesis of structurally complex natural products.In this article,we present a few examples for the elegant applications of the intermolecular D–A reaction that are inspired by biosynthetic hypotheses of the target natural products.These examples demonstrate that D–A reaction is not only useful for preparing building blocks but also powerful for coupling structurally complicated and sterically demanding segments in a highly chemo-and stereo-controlled fashion,which may inspire further developments of intermolecular D–A reaction from both strategy and methodology perspectives.展开更多
基金Supported by the National Natural Science Foundation of China(21376215)the National Science and Technology Major Project of New Drug,China(2012ZX09103101-075)+2 种基金the Innovative Research Platform co-constructed by Zhejiang University and Taizhou City,and the Science and Technology Project of Zhejiang Province(2014C33174)the Major State Basic Research Development Program of China(2011CB710803)the National High-Tech Research and Development Program of China(2012AA022302)
文摘Bacillus subtilis produces many chemlcally-dwerse seconaary metaDolltes or interest to chemists ano biologlsts. Based on this, this review gives a detailed overview of the natural components produced by B. subtilis including cyclic lipopeptides, polypeptides, proteins （enzymes）, and non-peptide products. Their structures, bioactive ac- tivities and the relevant variants as novel lead structures for drug discovery are also described. The challenging effects of fermentation metabolites, isolation and purification, as well as the overproduction of bioactive com- pounds from B. subtilis by metabolic engineering, ＇~ere also highlighted. Systematically exploring biosynthetic routes and the functions of secondary metabolites from 13. subtilis may not only be beneficial in improving yields of the products, but also in helping them to be used in food industry and public medical service on a large-scale.
基金financially supported by the Ministry of Science&Technology(2013CB836900)the National Natural Science Foundation of China(21290180,21172235,and 21222202)the Chinese Academy of Sciences
文摘The Diels–Alder(D–A)reaction is one of the most powerful reactions in organic synthesis.The intermolecular D–A reaction attracts much less attentions than its intramolecular counterpart in natural product synthesis,possibly due to the issues of reactivity and selectivity.In the past decade,the intermolecular D–A reaction has been increasingly utilized in the total synthesis of structurally complex natural products.In this article,we present a few examples for the elegant applications of the intermolecular D–A reaction that are inspired by biosynthetic hypotheses of the target natural products.These examples demonstrate that D–A reaction is not only useful for preparing building blocks but also powerful for coupling structurally complicated and sterically demanding segments in a highly chemo-and stereo-controlled fashion,which may inspire further developments of intermolecular D–A reaction from both strategy and methodology perspectives.
