NLRX1 (NLR family member X1)是细胞内模式识别受体NLRs家族中重要成员之一,在炎症反应和抗病毒等天然免疫中主要发挥负调控作用。为了解鸡NLRX1 (chNLRX1)分子的特征及其生物学功能,本研究采用cDNA末端快速克隆(RACE)技术,从鸡肺脏组...NLRX1 (NLR family member X1)是细胞内模式识别受体NLRs家族中重要成员之一,在炎症反应和抗病毒等天然免疫中主要发挥负调控作用。为了解鸡NLRX1 (chNLRX1)分子的特征及其生物学功能,本研究采用cDNA末端快速克隆(RACE)技术,从鸡肺脏组织中克隆了chNLRX1基因的cDNA序列,其最长开放阅读框(ORF)为2 970 bp,编码989个氨基酸,含有较为保守的结构域,分子构象分析显示chNLRX1在空间结构上更接近于鱼类NLRX1;将chNLRX1真核表达质粒转染HEK293T细胞和鸡DF-1细胞,显示chNLRX1主要定位于细胞质内的线粒体区;以腺病毒介导RNAi干扰chNLRX1表达,采用荧光显微镜观察和western blot方法筛选出干扰效率相对较高的si RNA (sh-NLRX1-2)。上述结果为进一步研究chNLRX1的生物学功能奠定基础。展开更多
Diabetic nephropathy(DN)is currently the most common complication of diabetes.It is considered to be one of the leading causes of end-stage renal disease(ESRD)and affects many diabetic patients.The pathogenesis of DN ...Diabetic nephropathy(DN)is currently the most common complication of diabetes.It is considered to be one of the leading causes of end-stage renal disease(ESRD)and affects many diabetic patients.The pathogenesis of DN is extremely complex and has not yet been clarified;however,in recent years,increasing evidence has shown the important role of innate immunity in DN pathogenesis.Pattern recognition receptors(PRRs)are important components of the innate immune system and have a significant impact on the occurrence and development of DN.In this review,we classify PRRs into secretory,endocytic,and signal transduction PRRs according to the relationship between the PRRs and subcellular compartments.PRRs can recognize related pathogen-associated molecular patterns(PAMPs)and danger-associated molecular patterns(DAMPs),thus triggering a series of inflammatory responses,promoting renal fibrosis,and finally causing renal impairment.In this review,we describe the proposed role of each type of PRRs in the development and progression of DN.展开更多
基金Project supported by the National Natural Science Foundation of China(Nos.81060063 and 81660129).
文摘Diabetic nephropathy(DN)is currently the most common complication of diabetes.It is considered to be one of the leading causes of end-stage renal disease(ESRD)and affects many diabetic patients.The pathogenesis of DN is extremely complex and has not yet been clarified;however,in recent years,increasing evidence has shown the important role of innate immunity in DN pathogenesis.Pattern recognition receptors(PRRs)are important components of the innate immune system and have a significant impact on the occurrence and development of DN.In this review,we classify PRRs into secretory,endocytic,and signal transduction PRRs according to the relationship between the PRRs and subcellular compartments.PRRs can recognize related pathogen-associated molecular patterns(PAMPs)and danger-associated molecular patterns(DAMPs),thus triggering a series of inflammatory responses,promoting renal fibrosis,and finally causing renal impairment.In this review,we describe the proposed role of each type of PRRs in the development and progression of DN.
