Natural resistance associated macrophage protein (Nramp) is an innate resistance protein to intracellular parasites, which is expressed plentifully in macrophage cells. Nramp has been studied in mouse, human, cattle, ...Natural resistance associated macrophage protein (Nramp) is an innate resistance protein to intracellular parasites, which is expressed plentifully in macrophage cells. Nramp has been studied in mouse, human, cattle, rainbow trout and channel catfish. However, little was known about the structure of Pagrus major Nramp. In order to get the complete sequence of Pagrus major Nramp, a pair of primer is designed according to a 200bp known sequence of Pagrus major Nramp cDNA. By the use of SMART RACE, the full Nramp of Pagrus major cDNA about 5 000 bp was obtained, including about 200 bp 5′ terminal region (UTR), complete encoding region and 3′ terminal region. There were 3 ployA signals, which showed many possibilities of cutting at 3′ terminal region. The character of Pagrus major Nramp nucleotide sequence and deduced amino acid sequence are analyzed. 12 putative transmembrane(TM) regions, a consensus transport motif (CTM), a predicted protein kinase C phosphroylation site and three predicted N-link glycosylation sites are indicated in its deduced amino acid sequence. The ’consense transport motif’ CTM is located between TM8 and TM9. Furthermore, a protein kinase C phosphroylation site and three N-link glycosylation sites were predicted. The alignment of amino acid sequences between Pagrus major Nramp cDNA and several animals is analyzed and the deduced amino acid sequence of Pagrus major Nramp had 77.8%, 83.0%, 82.3%, 80.0%, 81.1%, 60.4%, 70.3%, 58.5%, 69.5% identity with rainbow trout α(AAD20721), rainbow trout β (AAD20722), channel catfish(AF400108), fathead minnow (AAF01778), common carp (CAB60196), mouse 1(AAA39838), mouse 2(AAC42051), human 1(D50403), human 2(NP-000608), respectively. The alignment reveals high conservation in TM and CTM regions. Analysis result makes us get familiar with the structure and character of fish Nramp, furthermore, offers some information for the enhancement of immunity of fish and genetic amelioration on fish breeding.展开更多
Mycogen公司宣布,其Agrigenetics植物科学子公司开发出抗玉米蠕虫Diabrotica spp.(玉米生产中的主要害虫)的玉米植株。 Mycogen的总裁兼CEO Jerry Caulder指出:公司已对将天然玉米植物中的抗根蠕虫遗传物质导入其优质商商化杂种的方法...Mycogen公司宣布,其Agrigenetics植物科学子公司开发出抗玉米蠕虫Diabrotica spp.(玉米生产中的主要害虫)的玉米植株。 Mycogen的总裁兼CEO Jerry Caulder指出:公司已对将天然玉米植物中的抗根蠕虫遗传物质导入其优质商商化杂种的方法克发明申请专利保护。他预测种植对玉米根蠕虫具有天然抗性的种子将在4～5年内实现商品化。展开更多
文摘Natural resistance associated macrophage protein (Nramp) is an innate resistance protein to intracellular parasites, which is expressed plentifully in macrophage cells. Nramp has been studied in mouse, human, cattle, rainbow trout and channel catfish. However, little was known about the structure of Pagrus major Nramp. In order to get the complete sequence of Pagrus major Nramp, a pair of primer is designed according to a 200bp known sequence of Pagrus major Nramp cDNA. By the use of SMART RACE, the full Nramp of Pagrus major cDNA about 5 000 bp was obtained, including about 200 bp 5′ terminal region (UTR), complete encoding region and 3′ terminal region. There were 3 ployA signals, which showed many possibilities of cutting at 3′ terminal region. The character of Pagrus major Nramp nucleotide sequence and deduced amino acid sequence are analyzed. 12 putative transmembrane(TM) regions, a consensus transport motif (CTM), a predicted protein kinase C phosphroylation site and three predicted N-link glycosylation sites are indicated in its deduced amino acid sequence. The ’consense transport motif’ CTM is located between TM8 and TM9. Furthermore, a protein kinase C phosphroylation site and three N-link glycosylation sites were predicted. The alignment of amino acid sequences between Pagrus major Nramp cDNA and several animals is analyzed and the deduced amino acid sequence of Pagrus major Nramp had 77.8%, 83.0%, 82.3%, 80.0%, 81.1%, 60.4%, 70.3%, 58.5%, 69.5% identity with rainbow trout α(AAD20721), rainbow trout β (AAD20722), channel catfish(AF400108), fathead minnow (AAF01778), common carp (CAB60196), mouse 1(AAA39838), mouse 2(AAC42051), human 1(D50403), human 2(NP-000608), respectively. The alignment reveals high conservation in TM and CTM regions. Analysis result makes us get familiar with the structure and character of fish Nramp, furthermore, offers some information for the enhancement of immunity of fish and genetic amelioration on fish breeding.
文摘Mycogen公司宣布,其Agrigenetics植物科学子公司开发出抗玉米蠕虫Diabrotica spp.(玉米生产中的主要害虫)的玉米植株。 Mycogen的总裁兼CEO Jerry Caulder指出:公司已对将天然玉米植物中的抗根蠕虫遗传物质导入其优质商商化杂种的方法克发明申请专利保护。他预测种植对玉米根蠕虫具有天然抗性的种子将在4～5年内实现商品化。
