[Objective] This study was to raise the reproductive efficiency of a Dianjiang Peony variety Taipinghong. [Method] The Taipinghong was studied on the best selection and collection time of explant, the induction in the...[Objective] This study was to raise the reproductive efficiency of a Dianjiang Peony variety Taipinghong. [Method] The Taipinghong was studied on the best selection and collection time of explant, the induction in the early generation and the subculture multiplication. [Result] The best explant of Taipinghong was bulbil, and the best collecting time was March. The best induction recipe in early generation was modified MS medium （ammonium nitrate rate was reduced by half; calcium ion concentration doubled）＋arrageenan 6 g/L＋succharcose 30 g/L＋NAA 0.2 mg/L＋6-BA 2.0 mg/L＋GA3 0.5 mg/L＋AC 100 mg/L. [Conclusion] In the solution to browning phenomenon, both dark and AC （activated charcoal） treatments could obviously decrease the browning rate of explant.展开更多
文摘[Objective] This study was to raise the reproductive efficiency of a Dianjiang Peony variety Taipinghong. [Method] The Taipinghong was studied on the best selection and collection time of explant, the induction in the early generation and the subculture multiplication. [Result] The best explant of Taipinghong was bulbil, and the best collecting time was March. The best induction recipe in early generation was modified MS medium （ammonium nitrate rate was reduced by half; calcium ion concentration doubled）＋arrageenan 6 g/L＋succharcose 30 g/L＋NAA 0.2 mg/L＋6-BA 2.0 mg/L＋GA3 0.5 mg/L＋AC 100 mg/L. [Conclusion] In the solution to browning phenomenon, both dark and AC （activated charcoal） treatments could obviously decrease the browning rate of explant.
