Objective: To study the effect of methylprednisolone (MP) on reperfusion injury in severe uncontrolled hemorrhagic shock and explore the possible mechanism involved. Methods: Twelve dogs were randomly divided into two...Objective: To study the effect of methylprednisolone (MP) on reperfusion injury in severe uncontrolled hemorrhagic shock and explore the possible mechanism involved. Methods: Twelve dogs were randomly divided into two groups, control group (Group I, n=6) and MP group (Group II, n=6). The animals were bled continuously from a femoral artery catheter to produce uncontrolled hemorrhagic shock models. Resuscitation with lactated Ringer’s (LR) solution was initiated when mean arterial pressure (MAP) decreased to 20 mm Hg, and MAP was maintained at 30-40 mm Hg. MP (4 mg/kg) was injected intravenously in Group II when resuscitation began. While in Group I, normal saline (NS) was injected instead. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured before exsanguination (T 1), when MAP decreased to 20 mm Hg (T 2), 60 min (T 3) and 120 min (T 4) after resuscitation. Heart rate, MAP and cardiac output (CO) levels were recorded concomitantly. Results: Infusion volume and hemorrhage volume shed from the superior mesenteric artery in Group I were higher than those in Group II (P< 0.01 and P< 0.05). After reperfusion, blood SOD levels decreased progressively and MDA levels increased rapidly in Group I. In Group II, blood SOD levels at T 3 and T 4 decreased as compared with that at T 1 but a stepwise increase was present. At T 4, blood SOD level was significantly higher in Group II than in Group I (P< 0.01). At T 3 and T 4, MDA levels were markedly lower in Group II than in Group I. During reperfusion, MAP was more steady in Group II than in Group I and survival rate after 120 min (at T 4) was higher in Group II than in Group I (P< 0.05). Conclusions: MP has a protective effect on severe uncontrolled hemorrhagic shock and subsequent reperfusion injury. The mechanism mainly involves the anti-lipid peroxidation activity of MP.展开更多
OBJECTIVE： To observe the impact of total flavo- noids from Ganshanbian （Herba Hyperici Attenuati） on the expression of vascular smooth muscle mem-brane L-type calcium channel alpha1 C subunit （CaL-olC） and ATP-s...OBJECTIVE： To observe the impact of total flavo- noids from Ganshanbian （Herba Hyperici Attenuati） on the expression of vascular smooth muscle mem-brane L-type calcium channel alpha1 C subunit （CaL-olC） and ATP-sensitive K＋ channel （KATp）-Kir6.1 mRNA, and explore the mechanisms of the antiar- rhythmic effect of Ganshanbian （Herba Hyperici At- tenuatl~ total flavonoids. METHODS： The treatment vonoids from Ganshanbian group was fed total fla- （Herba Hyperici Attenua- ti） for 7 days by gavage with 100 mg. kg-~. d 1. The blank control group and model control group were given the same amount of normal saline for 7 do Ar- rhythmias were induced by performing a myocardi- al ischemia-reperfusion and electrocardiogram was observed. Reverse transcription-polymerase chain reaction was used to detect the expression of CaL-a 1Cand KATp-Kir6.1 mRNA in the myocardial cell mem- brane of all groups of rats. RESULTS： Total flavonoids from Ganshanbian （Her- ba Hyperici Attenuat can delay the appearance of myocardial ischemia reperfusion arrhythmias, shorten the duration of myocardial ischemia reper- fusion arrhythmias, reduce heart rate, reduce cell membrane expression of CaL-a1C mRNA and en- hance the expression of K_ATp-Kir6.1 mRNA in myocar- dial ischemia-reperfusion arrhythmic rats. CONCLUSION： Total flavonoids from Ganshanbian （Herba Hyperici Attenuat can alleviate arrhythmias by affecting the expression of L-type calcium chan- nels and ATP-sensitive K_ATP channels.展开更多
文摘Objective: To study the effect of methylprednisolone (MP) on reperfusion injury in severe uncontrolled hemorrhagic shock and explore the possible mechanism involved. Methods: Twelve dogs were randomly divided into two groups, control group (Group I, n=6) and MP group (Group II, n=6). The animals were bled continuously from a femoral artery catheter to produce uncontrolled hemorrhagic shock models. Resuscitation with lactated Ringer’s (LR) solution was initiated when mean arterial pressure (MAP) decreased to 20 mm Hg, and MAP was maintained at 30-40 mm Hg. MP (4 mg/kg) was injected intravenously in Group II when resuscitation began. While in Group I, normal saline (NS) was injected instead. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured before exsanguination (T 1), when MAP decreased to 20 mm Hg (T 2), 60 min (T 3) and 120 min (T 4) after resuscitation. Heart rate, MAP and cardiac output (CO) levels were recorded concomitantly. Results: Infusion volume and hemorrhage volume shed from the superior mesenteric artery in Group I were higher than those in Group II (P< 0.01 and P< 0.05). After reperfusion, blood SOD levels decreased progressively and MDA levels increased rapidly in Group I. In Group II, blood SOD levels at T 3 and T 4 decreased as compared with that at T 1 but a stepwise increase was present. At T 4, blood SOD level was significantly higher in Group II than in Group I (P< 0.01). At T 3 and T 4, MDA levels were markedly lower in Group II than in Group I. During reperfusion, MAP was more steady in Group II than in Group I and survival rate after 120 min (at T 4) was higher in Group II than in Group I (P< 0.05). Conclusions: MP has a protective effect on severe uncontrolled hemorrhagic shock and subsequent reperfusion injury. The mechanism mainly involves the anti-lipid peroxidation activity of MP.
基金Supported by National Natural Science Foundation of China(Proteomics research on the complexes of Ganshanbian(Herba Hyperici Attenuati)and Salvia miltiorrhiza anti-reperfusion arrhythmia based on the iTRAQ technology.No.81173185)Key Project of Natural Science Foundation of Heilongjiang Province(Proteomics research on the complexes of Ganshanbian(Herba Hyperici Attenuati)and Salvia miltiorrhiza anti-reperfusion arrhythmia based on the iTRAQ technology.No.ZD201111)+1 种基金Heilongjiang Province Graduate Students Research Key Project(Proteomics research on the complexes of Ganshanbian(Herba Hyperici Attenuati)and Salvia miltiorrhiza anti-reperfusion arrhythmia based on the iTRAQ technology.No.YJSCX2012-334HLJ)Outstanding Talents Support Program of Heilongjiang University of Chinese Medicine(Proteomics research on the complexes of Ganshanbian(Herba Hyperici Attenuati)and Salvia miltiorrhiza anti-reperfusion arrhythmia based on the iTRAQ technology,2012)
文摘OBJECTIVE： To observe the impact of total flavo- noids from Ganshanbian （Herba Hyperici Attenuati） on the expression of vascular smooth muscle mem-brane L-type calcium channel alpha1 C subunit （CaL-olC） and ATP-sensitive K＋ channel （KATp）-Kir6.1 mRNA, and explore the mechanisms of the antiar- rhythmic effect of Ganshanbian （Herba Hyperici At- tenuatl~ total flavonoids. METHODS： The treatment vonoids from Ganshanbian group was fed total fla- （Herba Hyperici Attenua- ti） for 7 days by gavage with 100 mg. kg-~. d 1. The blank control group and model control group were given the same amount of normal saline for 7 do Ar- rhythmias were induced by performing a myocardi- al ischemia-reperfusion and electrocardiogram was observed. Reverse transcription-polymerase chain reaction was used to detect the expression of CaL-a 1Cand KATp-Kir6.1 mRNA in the myocardial cell mem- brane of all groups of rats. RESULTS： Total flavonoids from Ganshanbian （Her- ba Hyperici Attenuat can delay the appearance of myocardial ischemia reperfusion arrhythmias, shorten the duration of myocardial ischemia reper- fusion arrhythmias, reduce heart rate, reduce cell membrane expression of CaL-a1C mRNA and en- hance the expression of K_ATp-Kir6.1 mRNA in myocar- dial ischemia-reperfusion arrhythmic rats. CONCLUSION： Total flavonoids from Ganshanbian （Herba Hyperici Attenuat can alleviate arrhythmias by affecting the expression of L-type calcium chan- nels and ATP-sensitive K_ATP channels.
