AIMTo test the effects of humic acids on innate microbial communities of the colon.METHODSWe followed the effects of oral supplementation with humic acids (Activomin<sup>®</sup>) on concentrati...AIMTo test the effects of humic acids on innate microbial communities of the colon.METHODSWe followed the effects of oral supplementation with humic acids (Activomin<sup>®</sup>) on concentrations and composition of colonic microbiome in 14 healthy volunteers for 45 d. 3 × 800 mg Activomin<sup>®</sup> were taken orally for 10 d followed by 3 × 400 mg for 35 d. Colonic microbiota were investigated using multicolor fluorescence in situ hybridization (FISH) of Carnoy fixated and paraffin embedded stool cylinders. Two stool samples were collected a week prior to therapy and one stool sample on days 10, 31 and 45. Forty-one FISH probes representing different bacterial groups were used.RESULTSThe sum concentration of colonic microbiota increased from 20% at day 10 to 30% by day 31 and remained stable until day 45 (32%) of humic acid supplementation (P < 0.001). The increase in the concentrations in each person was due to growth of preexisting groups. The individual microbial profile of the patients remained unchanged. Similarly, the bacterial diversity remained stable. Concentrations of 24 of the 35 substantial groups increased from 20% to 96%. Two bacterial groups detected with Bac303 (Bacteroides) and Myc657 (mycolic acid-containing Actinomycetes) FISH probes decreased (P > 0.05). The others remained unaffected. Bacterial groups with initially marginal concentrations (< 0.1 × 10<sup>9</sup>/mL) demonstrated no response to humic acids. The concentrations of pioneer groups of Bifidobacteriaceae, Enterobacteriaceae and Clostridium difficile increased but the observed differences were statistically not significant.CONCLUSIONHumic acids have a profound effect on healthy colonic microbiome and may be potentially interesting substances for the development of drugs that control the innate colonic microbiome.展开更多
Different from the traditional contact surface topography measurement,reflective intensity-modulated fiber optic sensor(RIM-FOS)has the unique advantages of non-contact nondestructive detection.This paper briefly intr...Different from the traditional contact surface topography measurement,reflective intensity-modulated fiber optic sensor(RIM-FOS)has the unique advantages of non-contact nondestructive detection.This paper briefly introduces the principle and performance of RIM-FOS for surface topography measurement and compares with several other methods of topography measurement.Based on the review of its development process,this paper summarizes and analyses the hot issues of RIM-FOS in the surface topography measurement,then predicts the future trend for a guidance of the further study.展开更多
Three common categories of bilateral asymmetry have been described： directional asymmetry, antisymmetry, and FA （fluctuating asymmetry）. FA is the most subtle of the three types of asymmetry, and differs from the p...Three common categories of bilateral asymmetry have been described： directional asymmetry, antisymmetry, and FA （fluctuating asymmetry）. FA is the most subtle of the three types of asymmetry, and differs from the patterns of the others in that paired structures tend to be symmetric in size. The analysis of FA allows to estimate the influence of stress factors on animal development and enables evaluation of resistance to stress. The aim of this work was to estimate the symmetry of skulls of a contemporary pure goat breed, as there is currently no study of FA as an estimate of individual quality for domestic goats. For this purpose, 11 landmarks were digitized in two dimensions from the crania of 24 adult goats （12 males and 12 females） belonging to the ＂White Rasquera＂ breed. The skulls showed nearly perfect bilateral symmetry. The low detected values of FA in skulls revealed a weak influence of developmental stress on this goat contemporary population and its strong ability to compensate stress.展开更多
The application of railway wheel noise compensation method is considered. The effect can be obtained by shift a pair of usual parallel rail joints on a pair of offset rail joints, for example, the right rail joint is ...The application of railway wheel noise compensation method is considered. The effect can be obtained by shift a pair of usual parallel rail joints on a pair of offset rail joints, for example, the right rail joint is shifted concerning the left rail joint on the certain distance. The distance is depend on the speed of train and promotes the correlation interconnection between excited acoustical pulses. The intensity wheel knock spectrograms corresponding of different distance between rail joints registered by the microphone are estimated. The spectrograms considerably differed from each other in low frequency band are analyzed. The result of practical using the acoustic noise compensation method is proved.展开更多
文摘AIMTo test the effects of humic acids on innate microbial communities of the colon.METHODSWe followed the effects of oral supplementation with humic acids (Activomin<sup>®</sup>) on concentrations and composition of colonic microbiome in 14 healthy volunteers for 45 d. 3 × 800 mg Activomin<sup>®</sup> were taken orally for 10 d followed by 3 × 400 mg for 35 d. Colonic microbiota were investigated using multicolor fluorescence in situ hybridization (FISH) of Carnoy fixated and paraffin embedded stool cylinders. Two stool samples were collected a week prior to therapy and one stool sample on days 10, 31 and 45. Forty-one FISH probes representing different bacterial groups were used.RESULTSThe sum concentration of colonic microbiota increased from 20% at day 10 to 30% by day 31 and remained stable until day 45 (32%) of humic acid supplementation (P < 0.001). The increase in the concentrations in each person was due to growth of preexisting groups. The individual microbial profile of the patients remained unchanged. Similarly, the bacterial diversity remained stable. Concentrations of 24 of the 35 substantial groups increased from 20% to 96%. Two bacterial groups detected with Bac303 (Bacteroides) and Myc657 (mycolic acid-containing Actinomycetes) FISH probes decreased (P > 0.05). The others remained unaffected. Bacterial groups with initially marginal concentrations (< 0.1 × 10<sup>9</sup>/mL) demonstrated no response to humic acids. The concentrations of pioneer groups of Bifidobacteriaceae, Enterobacteriaceae and Clostridium difficile increased but the observed differences were statistically not significant.CONCLUSIONHumic acids have a profound effect on healthy colonic microbiome and may be potentially interesting substances for the development of drugs that control the innate colonic microbiome.
基金Youth Science and Technology Research Foundation of Shanxi Province(No.2015021104)Programs for Science and Technology Development of Shanxi Province(No.201703D121028-2)
文摘Different from the traditional contact surface topography measurement,reflective intensity-modulated fiber optic sensor(RIM-FOS)has the unique advantages of non-contact nondestructive detection.This paper briefly introduces the principle and performance of RIM-FOS for surface topography measurement and compares with several other methods of topography measurement.Based on the review of its development process,this paper summarizes and analyses the hot issues of RIM-FOS in the surface topography measurement,then predicts the future trend for a guidance of the further study.
文摘Three common categories of bilateral asymmetry have been described： directional asymmetry, antisymmetry, and FA （fluctuating asymmetry）. FA is the most subtle of the three types of asymmetry, and differs from the patterns of the others in that paired structures tend to be symmetric in size. The analysis of FA allows to estimate the influence of stress factors on animal development and enables evaluation of resistance to stress. The aim of this work was to estimate the symmetry of skulls of a contemporary pure goat breed, as there is currently no study of FA as an estimate of individual quality for domestic goats. For this purpose, 11 landmarks were digitized in two dimensions from the crania of 24 adult goats （12 males and 12 females） belonging to the ＂White Rasquera＂ breed. The skulls showed nearly perfect bilateral symmetry. The low detected values of FA in skulls revealed a weak influence of developmental stress on this goat contemporary population and its strong ability to compensate stress.
文摘The application of railway wheel noise compensation method is considered. The effect can be obtained by shift a pair of usual parallel rail joints on a pair of offset rail joints, for example, the right rail joint is shifted concerning the left rail joint on the certain distance. The distance is depend on the speed of train and promotes the correlation interconnection between excited acoustical pulses. The intensity wheel knock spectrograms corresponding of different distance between rail joints registered by the microphone are estimated. The spectrograms considerably differed from each other in low frequency band are analyzed. The result of practical using the acoustic noise compensation method is proved.
