基于双链项头表的FP-growth改进算法

为减少事务数据库的扫描次数和提高FP-growth算法的伸缩性,本文提出利用双链项头表改进FP-growth算法。

CT头表改良定位穿刺术治疗高血压脑出血58例

目的改良CT头表定位方法,使穿刺靶点定位更精确。方法治疗58例高血压脑出血患者,剃发后在患侧头皮上贴两条定位标志线,行CT扫描,精确定位最佳穿刺靶点。结果靶点定位精确,穿刺引流成功。结论改良CT头表定位方法,更加精确,定位简便,快捷。引流血肿效果好。

不剃发颅内病灶CT头表定位

目的探索一种不剃发进行颅内病灶头表定位的方法。方法本组20例高血压性脑出血(基底节区血肿)病人,其中10例剃发后在发际外贴定位标志物,与此同时在同一病员的血肿附近贴定位标志物进行头表定位;另10例不剃头发(发际外贴标志物)进行头表定位。结果两种方法定位准确性完全一致,不剃发进行颅内病灶定位能在首次脑部CT扫描时同步完成。结论不剃头发颅内病灶CT头表定位,具有定位简便、快速、精确的特点,能充分满足一般急诊和微创手术病员的颅内病灶定位。

脑内病灶简易精确CT头表定位法

受日本学者工作的启发[1],近期我们利用CT机都具备的功能开发出一种更简易、精确的脑部病变体表投影CT定位方法,不需添加任何设备和软件,具有更简便易行,精度高,便于普及的优点,临床应用效果良好,报告如下.

用垂直数据格式构建FP增长树的算法

目前FP增长树的建立采用的是事务-项目集数据格式,即水平数据格式,扫描数据库需要2次。采用垂直数据格式,即项目-事务集数据格式,按照最小事务项目表优先的原则投影事务-项目以建立FP增长树,扫描数据库仅需1次。设计了独特的垂直项目头表独特的存储结构,便于垂直数据的存储、事务的投影、FP树的建立和垂直数据的增量更新。

可疑金融交易识别中的约束关联规则算法研究

在挖掘可疑金融数据中,提出一种快速的基于FP-tree的约束最大频繁项目集挖掘算法,把发现约束频繁项目集的问题转化为发现约束最大频繁项目集的问题。算法可以根据用户的需要,随时减少不满足约束条件的候选项目集的个数,提高运行效率,同时可确定挖掘主属性,确定生成的关联规则范围,为有效开展可疑金融交易识别提供了有益的参考。

Two new species in the bamboo-feeding genus Tiaobeinia(Hemiptera:Cicadellidae:Deltocephalinae)from China

Two new species of Mukariini,Tiaobeinia coarseata sp.nov.from Shaanxi and Tiaobeinia yuani sp.nov.from Gansu,are described.Detailed morphological descriptions and illustrations of these new species are given.A checklist of all known species in this tribe from China is provided and a key is proposed for all species of Tiaobeinia.

改进的频繁闭情节挖掘算法

为克服FCMMiner算法在挖掘频繁闭情节时存在的不足,基于最小且非重叠发生的支持度定义,提出一种事件序列上频繁闭情节挖掘算法FCM++。定义两种特殊的数据结构:频繁情节树(FET)、层头表(LH),采用广度优先搜索策略进行层扩展操作,扩展时将挖掘的频繁情节逐层压缩到FET和LH结点链中。通过动态维护FET及闭合性检查过程挖掘所有的频繁闭情节。实验结果表明,FCM++算法较FCMMiner算法有更高的挖掘效率,能有效地挖掘所有的频繁闭情节。

In-vivo characterization of DALM in ulcerative colitis with high-resolution probe-based confocal laser endomicroscopy

Recently,the use of confocal laser endomicroscopy(CLE) in the diagnosis of chronic ulcerative colitis(CUC) was reported.In this brief report we aimed to assess the application of probe-based CLE to characterize colonic mucosa and dysplasia in CUC.The study involved a patient presenting long-standing CUC.Confocal imaging of both the inflamed mucosa,a circumscribed lesion(dysplasiaassociated lesional mass),and adjacent colonic mucosa are demonstrated and the correlation between the CLE and histological images.Inflamed mucosa and dysplasia showed specific alteration of crypt architecture,cellular infiltration,and vessel architecture with an excellent correlation between CLE and standard histological examination.

THE PHYSICAL MODEL AND MATH MODEL OF CONTACT FAILURE ON SMALL CURRENT CONTACTS UNDER MECHANICAL ACTIONS

Based on the experimental results of the references [1] and [2], i. e., the contact resistance increases due to the contaminants formed on contact surface with the operating cycles,the physical model and math model of contact failure on small current contacts under mechanical actions are proposed through deep theoretical analysis in this paper.

Preliminary Assessment of Genetic Diversity in Three Taro （Colocasia esculenta L, Schott） Landraces Using Agro-morphological and SSR DNA Characterisation

Taro （Colocasia esculenta L. Schott） is an important underutilised crop in South Africa, East Africa and Indonesia. Three taro landraces, namely, Dumbe Lomfula （wild）, KwaNgwanase and Umbumbulu, were collected from two locations in KwaZulu-Natal （KZN）, South Africa, and planted at two locations, Pietermaritzburg （KZN） and Roodeplaat, Pretoria. Ago-morphological characterisation of vegetative and corm characteristics were done four months after planting and at harvest, respectively. Sampling for DNA fingerprinting using five SSR primers was done using leaf material four months after planting. Agro-morphological characterisation was useful in showing differences between the wild landrace and the two cultivated landraces, as well as identification of dasheen and eddoe types. SSR primer characterisation showed that despite significant morphological difference, the wild Dumbe Lomfula and Umbumbulu landraces were closely related but different from the KwaNgwanase landrace. Although landraces showed great morphological variation, this did not necessarily imply genetic variation. It is concluded that SSR primers are more useful for characterising taro landraces.

Molecular cloning,tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding

Mucins are important components of mucus, which form a natural, physical, biochemical and semipermeable mucosal layer on the epidermis offish gills, skin, and the gastrointestinal tract. As the first step towards characterizing the function of Muc2, we cloned a partial Megalobrama amblycephala Muc2 cDNA of 2 175 bp, and analyzed its tissue-specific expression pattern by quantitative real-time PCR （qPCR）. The obtained sequence comprised 41 bp 5＇-untranslated region （5＇-UTR）, 2 134 bp open reading frame encoding a protein of 711 amino acids. BLAST searching and phylogenetic analysis showed that the predicted protein contained several common secreted mucin-module domains （VWD-C8-TIL-VWD-C8） and had high homology with mucins from other vertebrates. Among four candidate reference genes （β-Actin, RP113a, RPII, 18S） for the qPCR, RPII was chosen as an appropriate reference gene because of its lowest variation in different tissues. M. amblycephala Muc2 was mainly expressed in the intestine, in the order （highest to lowest） middle-intestine 〉 fore-intestine 〉 hind-intestine. Muc2 was expressed relatively poorly in other organs （brain, liver, kidney, spleen, skin and gill）. Furthermore, after 20-days of starvation, M. amblycephala Muc2 expressions after refeeding for 0 h, 3 h, 16 h, 3 d, and 10 d were significantly decreased in the three intestinal segments （P〈0.05） at 16 h, and were then upregulated to near the initial level at 10 d.

Regulation of apoptosis by the papillomavirus E6 oncogene

Infection with human papillomaviruses is strongly associated with the development of multiple cancers including esophageal squarnous cell carcinoma. The HPV E6 gene is essential for the oncogenic potential of HPV. The regulation of apoptosis by oncogene has been related to carcinogenesis closely; therefore, the modulation of E6 on cellular apoptosis has become a hot research topic recently. Inactivation of the pro-apoptotic tumor suppressor p53 by E6 is an important mechanism by which E6 promotes cell growth; it is expected that inactivation of p53 by E6 should lead to a reduction in cellular apoptosis, numerous studies showed that E6 could in fact sensitize cells to apoptosis. The molecular basis for apoptosis modulation by E6 is poorly understood. In this article, we will present an overview of observations and current understanding of molecular basis for E6-induced apoptosis.

One new species in the genus Nephrocerus Zetterstedt(Diptera:Pipunculidae),with a key to species from the Palaearctic Region

One new species, Nephrocerus bullatus sp. nov., is described from China. A key to species of this genus from the Palaearctic Region is presented.

Closure behavior of rock joint under dynamic loading

The normal compression tests on intact samples and artificial joints with different saw-tooth shape under cyclic loading and half-sine waves of different frequencies were performed by using Instron1342 servo-controlled material testing machine. The specimens were made artificially with mortar. The loading frequency ranged from 0.005 Hz to 0.1 Hz. The experimental results show that joint closure curves are non-linear and concave up. The stress-deformation curves under cyclic loading exhibit hysteresis and permanent set that diminish rapidly and keep constant finally on successive cycles. Normal displacement successively decreases from the joint J1 to J2, to J3 under the same normal loads regardless of frequency. Considering the loading frequency effect, normal displacement of joint J1 decreases with increasing the loading frequency except that the loading frequency is 0.05 Hz. Normal displacement of joint J2 increases with increasing the loading frequency. Normal displacement of joint J3 increases with increasing the loading frequency when the frequency ranges from 0.005 Hz to 0.05 Hz. Its normal displacement, however, becomes least when the loading frequency is 0.1 Hz.

Research progress of microRNA in prevention and treatment of osteonecrosis of the femoral head

In the 1980s,Feinbaum et al.first discovered miRNA in the development of worms and termed it as Lin-4.With the development of microbial science and continuous improvement in the gene sequencing technology,miRNA has been gradually gaining attention for intensive research in clinical medicine.Among these,one group of miRNAs has been confirmed to play fundamental roles in gene regulation in various orthopedics diseases,such as bone tumors,osteoarthritis,and rheumatoid arthritis.The study of miRNAs in the osteonecrosis of the femoral head(ONFH)can improve the understanding of the pathogenesis of the disease.ONFH is an orthopedic disease that is the primary cause of disrupted blood supply to the femoral head and the main symptoms of bone and muscle dysfunction.Recent studies showed that miRNA played a major role in the regulation of the microcirculation of ONFH,damage and repair of blood vessels,local microcirculation dysfunction caused by other diseases,and apoptosis of bone cells.In this study,recent related research results of miRNA and ONFH were analyzed and summarized,and the prospective in the prevention and treatment of the disease was also discussed.

MSCT and MRCP features of intraductal papillary mucinous tumor of the pancreas

Objective:To study the multi-slice CT(MSCT) and magnetic resonance cholangio-pancreatography(MRCP) features of intraductal papillary mucinous tumor(IPMT) of the pancreas.Methods:The clinical information,laboratory val-ues,imaging features and pathological findings of 8 cases were reviewed.Results:There were 6 males and 2 females with average 71.3 years old in this series.These lesions involved pancreatic branch ducts or main duct.Among them,2 cases were involved in the pancreatic uncinate process,1 case in the pancreatic head,1 in the pancreatic body,2 in the pancreatic tail and 2 cases involved multiple pancreatic duct.The common complaint was chronic upper abdominal pain.The imaging features included cystic lesion and it's association with the dilated pancreatic main duct,and bulging of the duodenal papilla.Mucin plug,septa or mural nodule were found in these cystic lesions.5 cases were confirmed with endoscopic retrograde cholangio-pancreatography(ERCP).3 cases had undergone surgery.Pathological findings of the 3 cases were adenoma,adenocarcinoma and borderline tumor,respectively.Conclusion:The MSCT and MRCP features of IPMT are somewhat characteristic,and the correct diagnosis can be made with the combination of clinical features.Diameter of branch duct ≥ 3 cm with multiple or large mural nodules,or severe dilated main duct indicates the probability of malignant IPMT.

SPECIFIC UP-REGULATION OF DNA POLYMERASE BY HUMAN PAPILLOMAVIRUS 16

Obje, etive To analyze how the infection of human papillomavirus 16 （ HPV16 ） affects expression of DNA poly- merase β（DNA polB） with the aim of probing the mechanism of over-expression of DNA polB in human cancers. Methods Four fragments of human DNA polB promoter were amplified and constructed into luciferase reporter vec- tor pGL-Basic, generating pGL-BP, pGL-BMH, pGL-BMS, and pGL-BAT constructs respectively, and co-transfected with HPV16 or HPV6 into Hep2 cells. Luciferase activity was assayed 48 hours after transfectiorL Semi-quantitative RT- PCR was used to measure mRNA expression of endogenous DNA polB. Immunohistochemistry and in situ hybridization were used to analyze DNA polB expression and HPV16 or HPV6 infection in 38 cases of cervical lesions respectively. Results With co-transfection of HPV16 and DNA polB promoter-driving reporters into Hep2 cells, pGL-BP re- porter in full-length DNA polB promoter presented markedly elevated luciferase activities （ P 〈 0.05 ）. However, the other three mutant reporters： pGL-BMH, pGL-BMS, and pGL-BAT, generated no reporting activities in the presence of HPV16 （P 〉0.05 ）. On the contrary, all of polB promoter reporters were little stimulated in co-transfection of HPV6 （P 〉0.05 ）. The transfection of HPV16 could enhance the endogenous polB mRNA expression compared with that of HPV6 （3.42 vs. 0.80, P 〈0.05）. The DNA polB expression was found in 8 of 10 HPV16-positive cervical intraepi- thelial neoplasia grade Ⅲ（ CIN Ⅲ） cases, while was only found in 3 of 11 HPV6-positive condyloma accuminatum cases, but was negative in all chronic cervicitis cases. The correlation of DNA polB expression with HPV16 infection in cervical lesions was significant （ P 〈0.05 ）. Conclusions HPV16 is able to specifically stimulate the expression of DNA polB in human epithelial cells through interaction with the core upstream regulatory sequences of DNA polB promoter. Over-expression of DNA polB might be an explanation for the molecular mechanism underlying HPV-related human cancers.

Port and Marine Structures Made of Sheet Piling with Staggered Toe

Some new approaches to designing and calculation of maritime structures made of sheet piling with staggered toe are considered and discussed. Obtained results allow determination of piles spacing efficiency in staggered embedment wall. The specificity of interaction of piles in ＂comb＂ with the soil foundation Practical application is illustrated by example of calculation. regarding transition from continuous to ＂comb＂ wall is investigated.

Screening and analysis of differentially expressed genes of dermal papillae cells with aggregative behavior

Objective: To screen and clone differentially expressed genes of dermal papillae cells (DPC) with aggregative behavior, and to explore the molecular mechanism of their aggregation. Methods: Total RNAs were extracted from DPC with and without aggregative behavior and double strand cDNAs were synthesized by using SMART cDNA synthesis, respectively. The cDNA fragments of differentially expressed genes in DPCs with aggregative behavior were isolated by suppression subtractive hybridization. Positive clones were screened by PCR method and verified by cDNA dot blot, Northern blot and then analyzed through homologous retrieving. Results: A subtractive cDNA library of DPC with aggregative behavior has been successfully constructed. The result of screening and cloning of the library showed that, DPC with aggregative behavior could expresse genes related to homologous aggregation, proliferation and cycle control, including known genes (capping protein, paladin, vascular endothelial growth factor), hematopoietic stem/progenitor cells (HSPC) related clone (HSPC011 and HSPC016) and a new gene. Conclusion: The construction of subtracted library of DPC lays solid foundation for screening and cloning new and specific genes related to aggregative behavior of DPC. Several genes might be cooperatively involved in the homologous aggregation, proliferation and cycle control of DPC. Among these genes, capping protein and palladin might be closely related to the aggregative behavior of dermal papilla cells, and VEGF and HSPC related clone would be responsible for the status of higher proliferation of dermal papilla cells.