Objective:To investigate the effect of Zi Chong Granules(资冲颗粒)on estradiol(E_2)synthesis in the cultured ovarian granular cells of the mice,so as to probe into the mechanism of the drug in promoting follicular dev...Objective:To investigate the effect of Zi Chong Granules(资冲颗粒)on estradiol(E_2)synthesis in the cultured ovarian granular cells of the mice,so as to probe into the mechanism of the drug in promoting follicular development.Methods:Testosterone(T)with different concentration were added to the cultured ovarian granular cells,and 24 and 48 hours later the enzyme immunoassay(EIA)was used to determine the required concentration of T and time for E_2 synthesis;Zi Chong Granules,its active components for tonifying the kidney and removing blood stasis,and the mouse serum containing Zi Chong Granules were respectively added to the medium of the cultured mouse ovarian granular cells and the mixtures were cultured for 48 hours to determine the E_2 concentration in various cultures,with insulin growth factor-1(IGF-1),follicle-stimulating hormone(FSH)and NJin Dan(女金丹丸 Golden Pill for Women)as positive control drugs.Results:Zi Chong Granules and NJin Dan(were found to be able to increase the E_2 level in the medium of the cultured ovarian granular cells to a certain extent,and the active components for tonifying the kidney or those for removing the blood stasis did not increase the E_2 level, but the mouse serum containing Zi Chong Granules could significantly increase the level of E_2. Conclusion:To promote the E_2 synthesis in the ovarian granular cells is one of mechanisms of Zi Chong Granules in stimulating follicular development.展开更多
文摘Objective:To investigate the effect of Zi Chong Granules(资冲颗粒)on estradiol(E_2)synthesis in the cultured ovarian granular cells of the mice,so as to probe into the mechanism of the drug in promoting follicular development.Methods:Testosterone(T)with different concentration were added to the cultured ovarian granular cells,and 24 and 48 hours later the enzyme immunoassay(EIA)was used to determine the required concentration of T and time for E_2 synthesis;Zi Chong Granules,its active components for tonifying the kidney and removing blood stasis,and the mouse serum containing Zi Chong Granules were respectively added to the medium of the cultured mouse ovarian granular cells and the mixtures were cultured for 48 hours to determine the E_2 concentration in various cultures,with insulin growth factor-1(IGF-1),follicle-stimulating hormone(FSH)and NJin Dan(女金丹丸 Golden Pill for Women)as positive control drugs.Results:Zi Chong Granules and NJin Dan(were found to be able to increase the E_2 level in the medium of the cultured ovarian granular cells to a certain extent,and the active components for tonifying the kidney or those for removing the blood stasis did not increase the E_2 level, but the mouse serum containing Zi Chong Granules could significantly increase the level of E_2. Conclusion:To promote the E_2 synthesis in the ovarian granular cells is one of mechanisms of Zi Chong Granules in stimulating follicular development.
