Under conditions of a warmer climate,the advance of the alpine treeline into alpine tundra has implications for carbon dynamics in mountain ecosystems.However,the above- and below-ground live biomass allocations among...Under conditions of a warmer climate,the advance of the alpine treeline into alpine tundra has implications for carbon dynamics in mountain ecosystems.However,the above- and below-ground live biomass allocations among different vegetation types within the treeline ecotones are not well investigated.To determine the altitudinal patterns of above-/below-ground carbon allocation,we measured the root biomass and estimated the above-ground biomass(AGB) in a subalpine forest,treeline forest,alpine shrub,and alpine grassland along two elevational transects towards the alpine tundra in southeast Tibet.The AGB strongly declined with increasing elevation,which was associated with a decrease in the leaf area index and a consequent reduction in carbon gain.The fine root biomass(FRB) increased significantly more in the alpine shrub and grassland than in the treeline forest,whereas the coarse root biomass changed little with increasing altitudes,which led to a stable below-ground biomass(BGB) value across altitudes.Warm and infertile soil conditions might explain the large amount of FRB in alpine shrub and grassland.Consequently,the root toshoot biomass ratio increased sharply with altitude,which suggested a remarkable shift of biomass allocation to root systems near the alpine tundra.Our findings demonstrate contrasting changes in AGB and BGB allocations across treeline ecotones,which should be considered when estimating carbon dynamics with shifting treelines.展开更多
With continuous and slow oxygen-release characteristic, calcium peroxide (CaO2) has been a new source of supplying oxygen for aerobic microbes in bioremediation of contaminated groundwater. Batch experiments were co...With continuous and slow oxygen-release characteristic, calcium peroxide (CaO2) has been a new source of supplying oxygen for aerobic microbes in bioremediation of contaminated groundwater. Batch experiments were conducted to evaluate the oxygen-release rate of CaO2reacting with water, the regulation of high pH, as well as the growth of mixed aerobic microbes in the medium containing CaO2, The results show that the oxygen-release process of CaO2 comprises three phases. In the first phase, dissolved oxygen levels of water increased sharply, and average oxygen-release rates increased as the adding weight of CaO2 increased, However, the rates almost kept invariable in the latter phases and were approximately 0.48; and 0. 084 mg O2·h^-1, respectively. As the necessary components of medium, potassium dihydrogen phosphate (KH2PO4 ) and amrnoniurn sulphate ((NH4)2SO4) at a certain ratio could regulate pH caused by CaO2 from 12, 1 to the range of 6.5 -8.5, which is helpful for microbial growth, In addition, diauxic growth curve observed in the medium containing CaO2 suggested that the growth of mixed aerobic microbes could be stimulated by the addition of CaO2.展开更多
Di-n-butyl phthalate (DBP),one of phthalate acid esters (PAEs),was investigated to determine its biodegradation rate using Xiangjiang River sediment and find potential DBP degraders in the enrichment culture of the se...Di-n-butyl phthalate (DBP),one of phthalate acid esters (PAEs),was investigated to determine its biodegradation rate using Xiangjiang River sediment and find potential DBP degraders in the enrichment culture of the sediment. The sediment sample was incubated with an initial concentration of DBP of 100 mg/L for 5 d. The biodegradation rate of DBP was detected using HPLC and the degraded products were analyzed by GC/MS. Subsequently,the microbial diversity of the enrichment culture was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results reveal that almost 100% of DBP is degraded after merely 3 d,generating two main degraded products:mono-butyl phthalate (MBP) and 9-octadecenoic acid. After a six-month enrichment period under the pressure of DBP,the dominant family in the final enrichment culture is clustered with the Comamonas sp.,the remaining are affiliated with Sphingomonas sp.,Hydrogenophaga sp.,Rhizobium sp.,and Acidovorax sp. The results show the potential of these bacteria to be used in the bioremediation of DBP in the environment.展开更多
Increased attention has been given to the fate of pollutants such as polycyclic aromatic hydrocarbons (PAHs) introduced to the wastewater treatment plants.Dissolved and adsorbed PAHs were detected in the centralized w...Increased attention has been given to the fate of pollutants such as polycyclic aromatic hydrocarbons (PAHs) introduced to the wastewater treatment plants.Dissolved and adsorbed PAHs were detected in the centralized wastewater treatment plant of a chemical industry zone in Zhejiang Province,China.The most abundant PAHs were the low molecular weight PAHs (e.g.,Acy,Ace,Flu and Phe),accounting for more than 80% of the total 16 PAHs in each treatment stage.Phase partitioning suggested that the removal of PAHs in every treatment stage was influenced greater by the sorption of particles or microorganisms.The removal efficiencies of individual PAHs ranged between 4% and 87% in the primary sedimentation stage,between 1% and 42% in anaerobic hydrolysis stage,between <1% and 70% in aerobic bio-process stage,between 1.5% and 80% in high-density clarifier stage,and between 44% and 97% in the whole treatment process.Mass balance calculations in primary stage showed significant losses for low molecular weight PAHs and relatively good agreements for high molecular weight PAHs as well as in anaerobic hydrolysis,high-density clarifier stage and sludge stream for most PAHs.Great gains of 60%-150% were obtained for high molecular weight PAHs in aerobic bio-process stage due to biosorption and bioaccumulation.Our investigations found that PAHs entering the wastewater treatment plant (WWTP) could be derived from the dyeing chemical processes as the byproducts,and the contribution supported by the largest dyeing chemical group was up to 48%.展开更多
Understanding the mechanisms underlying the assembly of nucleobases is a great challenge. The ability to deeply understand how nucleobases interact with themselves as well as with other molecules will allow us to gain...Understanding the mechanisms underlying the assembly of nucleobases is a great challenge. The ability to deeply understand how nucleobases interact with themselves as well as with other molecules will allow us to gain valuable insights into how we might be able to harness these interesting biological molecules to construct complex nanostructures and materials. Uracil and thymine derivatives have been reported for use in biological applications and in self-assembling triple hydrogen bonded systems. Either uracil or thymine possesses three binding sites (Site 1, Site 2, and Site 3) that can induce strong directional N-H...O=C hydrogen bonding interaction. In this paper, theoretical calculations are carded out on the structural features and binding energies of hydrogen-bonded dimers and trimers formed by uracil and thymine bases. We find that the hydrogen bonds formed through Site 1 are the strongest, those formed through Site 3 are next, while those formed through Site 2 are the weakest. The atoms in molecules analysis show that the electron densities at the bond critical points and the corresponding Laplacians have greater values for those hydrogen bonds formed through Site 1 than through Site 2. All these results indicate that a uracil (or thymine) would interact with another uracil or thymine most likely through Site 1 and least likely through Site 2. We also find that a simple summation rule roughly exists for the binding energies in these dimers and trimers.展开更多
基金supported by the National Natural Science Foundation of China (41561011)the Natural Science Foundation of Jiangxi Province, China (20151BAB213029)
文摘Under conditions of a warmer climate,the advance of the alpine treeline into alpine tundra has implications for carbon dynamics in mountain ecosystems.However,the above- and below-ground live biomass allocations among different vegetation types within the treeline ecotones are not well investigated.To determine the altitudinal patterns of above-/below-ground carbon allocation,we measured the root biomass and estimated the above-ground biomass(AGB) in a subalpine forest,treeline forest,alpine shrub,and alpine grassland along two elevational transects towards the alpine tundra in southeast Tibet.The AGB strongly declined with increasing elevation,which was associated with a decrease in the leaf area index and a consequent reduction in carbon gain.The fine root biomass(FRB) increased significantly more in the alpine shrub and grassland than in the treeline forest,whereas the coarse root biomass changed little with increasing altitudes,which led to a stable below-ground biomass(BGB) value across altitudes.Warm and infertile soil conditions might explain the large amount of FRB in alpine shrub and grassland.Consequently,the root toshoot biomass ratio increased sharply with altitude,which suggested a remarkable shift of biomass allocation to root systems near the alpine tundra.Our findings demonstrate contrasting changes in AGB and BGB allocations across treeline ecotones,which should be considered when estimating carbon dynamics with shifting treelines.
基金Supported by National Natural Science Foundation of China (No. 20276048).
文摘With continuous and slow oxygen-release characteristic, calcium peroxide (CaO2) has been a new source of supplying oxygen for aerobic microbes in bioremediation of contaminated groundwater. Batch experiments were conducted to evaluate the oxygen-release rate of CaO2reacting with water, the regulation of high pH, as well as the growth of mixed aerobic microbes in the medium containing CaO2, The results show that the oxygen-release process of CaO2 comprises three phases. In the first phase, dissolved oxygen levels of water increased sharply, and average oxygen-release rates increased as the adding weight of CaO2 increased, However, the rates almost kept invariable in the latter phases and were approximately 0.48; and 0. 084 mg O2·h^-1, respectively. As the necessary components of medium, potassium dihydrogen phosphate (KH2PO4 ) and amrnoniurn sulphate ((NH4)2SO4) at a certain ratio could regulate pH caused by CaO2 from 12, 1 to the range of 6.5 -8.5, which is helpful for microbial growth, In addition, diauxic growth curve observed in the medium containing CaO2 suggested that the growth of mixed aerobic microbes could be stimulated by the addition of CaO2.
基金Project(50621063) supported by the National Nature Science Foundation of ChinaProject(NCET-06-0691) supported by the Program for New Century Excellent Talents in University
文摘Di-n-butyl phthalate (DBP),one of phthalate acid esters (PAEs),was investigated to determine its biodegradation rate using Xiangjiang River sediment and find potential DBP degraders in the enrichment culture of the sediment. The sediment sample was incubated with an initial concentration of DBP of 100 mg/L for 5 d. The biodegradation rate of DBP was detected using HPLC and the degraded products were analyzed by GC/MS. Subsequently,the microbial diversity of the enrichment culture was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results reveal that almost 100% of DBP is degraded after merely 3 d,generating two main degraded products:mono-butyl phthalate (MBP) and 9-octadecenoic acid. After a six-month enrichment period under the pressure of DBP,the dominant family in the final enrichment culture is clustered with the Comamonas sp.,the remaining are affiliated with Sphingomonas sp.,Hydrogenophaga sp.,Rhizobium sp.,and Acidovorax sp. The results show the potential of these bacteria to be used in the bioremediation of DBP in the environment.
基金support to this study provided by National Key Technology R&D Program of China (2008BAC32B06)the National Natural Science Foundation of China (21076188,20836008, 20976158,20990221 & 21076189)the Key Innovation Team for Science and Technology of Zhejiang Province of China (2009R50047)
文摘Increased attention has been given to the fate of pollutants such as polycyclic aromatic hydrocarbons (PAHs) introduced to the wastewater treatment plants.Dissolved and adsorbed PAHs were detected in the centralized wastewater treatment plant of a chemical industry zone in Zhejiang Province,China.The most abundant PAHs were the low molecular weight PAHs (e.g.,Acy,Ace,Flu and Phe),accounting for more than 80% of the total 16 PAHs in each treatment stage.Phase partitioning suggested that the removal of PAHs in every treatment stage was influenced greater by the sorption of particles or microorganisms.The removal efficiencies of individual PAHs ranged between 4% and 87% in the primary sedimentation stage,between 1% and 42% in anaerobic hydrolysis stage,between <1% and 70% in aerobic bio-process stage,between 1.5% and 80% in high-density clarifier stage,and between 44% and 97% in the whole treatment process.Mass balance calculations in primary stage showed significant losses for low molecular weight PAHs and relatively good agreements for high molecular weight PAHs as well as in anaerobic hydrolysis,high-density clarifier stage and sludge stream for most PAHs.Great gains of 60%-150% were obtained for high molecular weight PAHs in aerobic bio-process stage due to biosorption and bioaccumulation.Our investigations found that PAHs entering the wastewater treatment plant (WWTP) could be derived from the dyeing chemical processes as the byproducts,and the contribution supported by the largest dyeing chemical group was up to 48%.
基金supported by the National Natural Science Foundation of China (20973088)the Educational Department of Liaoning Province (2007T091, 2008T106)
文摘Understanding the mechanisms underlying the assembly of nucleobases is a great challenge. The ability to deeply understand how nucleobases interact with themselves as well as with other molecules will allow us to gain valuable insights into how we might be able to harness these interesting biological molecules to construct complex nanostructures and materials. Uracil and thymine derivatives have been reported for use in biological applications and in self-assembling triple hydrogen bonded systems. Either uracil or thymine possesses three binding sites (Site 1, Site 2, and Site 3) that can induce strong directional N-H...O=C hydrogen bonding interaction. In this paper, theoretical calculations are carded out on the structural features and binding energies of hydrogen-bonded dimers and trimers formed by uracil and thymine bases. We find that the hydrogen bonds formed through Site 1 are the strongest, those formed through Site 3 are next, while those formed through Site 2 are the weakest. The atoms in molecules analysis show that the electron densities at the bond critical points and the corresponding Laplacians have greater values for those hydrogen bonds formed through Site 1 than through Site 2. All these results indicate that a uracil (or thymine) would interact with another uracil or thymine most likely through Site 1 and least likely through Site 2. We also find that a simple summation rule roughly exists for the binding energies in these dimers and trimers.