妊娠期高血压疾病胎盘滋养细胞中缺氧诱导因子的表达及意义

目的:通过观察胎盘滋养细胞中缺氧诱导因子-Ⅰα(HIF-1α)在妊娠期高血压疾病孕妇与正常孕妇胎盘中的表达差异,探讨其在妊娠期高血压疾病发病中的作用。方法:采用免疫组化法检测112例妊娠期高血压疾病患者(观察组)及110例正常孕妇(对照组)胎盘组织中的HIF-1α蛋白的表达。结果:妊娠期高血压疾病组缺氧诱导因子HIF-1α的表达明显高于正常妊娠组。妊娠高血压疾病组中,病情较重,阳性表达率越高,三组两两比较,均有统计学差异。结论:妊娠期高血压疾病患者胎盘组织HIF-1α明显高于正常孕妇,且随患者病情加重其表达明显升高,说明缺氧诱导因αHIF-1α在妊娠期高血压疾病发病中起重要作用。

妊娠期糖尿病孕妇外周血单个核细胞TNF-α基因表达水平的实验性研究

目的:观察妊娠期糖尿病(GDM)患者外周血单个核细胞(PBM C)中肿瘤坏死因子-α(TNF-α)基因转录和表达的水平,探讨其在GDM发病过程中的意义。方法:选取GDM、正常妊娠和健康非妊娠组各30例,采用EL ISA法检测空腹血清中的TNF-α水平。收集研究对象外周血单个核细胞(PBM C),采用半定量逆转录-聚合酶链反应(RT-PCR)和免疫印记分析(W estern b lot)技术测定实验对象外周血单个核细胞TNF-αmRNA转录和蛋白质表达的水平。结果:GDM组、正常妊娠组空腹血清TNF-α水平显著高于健康非妊娠组,而GDM组显著高于正常妊娠组。三组间的外周血单个核细胞TNF-αmRNA转录水平、TNF-α蛋白的相对含量无显著性差异。结论:TNF-α可能参与了GDM的发生发展过程,但是PBM C可能不是GDM时TNF-α增加的来源。

Placental expressions of estrogen receptor α,estrogen receptor β in intrahepatic cholestasis of pregnancy

To investigate the association of the expression of estrogen receptor ct, estrogen receptor 13 in placenta with intrahepatic cholestasis of pregnancy （ICP） susceptibility. Methods： In 14 cases of mild ICP, 14 cases of severe ICP and 14 cases of normal cases （control group） with corresponding age and gestation weeks, the expressions of ERa and ERD were detected by means of immunohistochemical method S-P. Results： The mean grey numbers of ERa in each group mentioned above were 151.684±3.76, 149.854±3.69, 153.184±3.18, without significant difference （P〉0.05） The mean grey numbers of ERβ in each group mentioned above were 146.51±3.81, 139.434±9.97, 149.87±4.17, with significant difference （P〉0.05）; the expression of ERI3 of severe ICP group was significantly higher than that of the mild ICP group and the control group （P〈0.05）. The expression of ERβ in every group was higher than that of ERa （P〈0.05）. Conclusion： ERβ maybe play an important part in the etiology and development of ICP