AIM： To enrich hepatic progenitors using epithelial cell adhesion molecule （EpCAM） as a marker from human fetal liver and investigate the expression of human leukocyte antigen （HLA） and their markers associated w...AIM： To enrich hepatic progenitors using epithelial cell adhesion molecule （EpCAM） as a marker from human fetal liver and investigate the expression of human leukocyte antigen （HLA） and their markers associated with hepatic progenitor cells. METHODS： EpCAM ＋ve cells were isolated using magnetic cell sorting （MACS） from human fetuses （n = 10） at 15-25 wk gestation. Expression of markers for hepatic progenitors such as albumin, alpha-fetoprotein （AFP）, CD29 （integrin ~1）, CD49f （integrin c^6） and CD90 （Thy 1） was studied by using flow cytometry, immunocytochemistry and RT-PCR; HLA class Ⅰ （A, B, C） and class Ⅱ （DR） expression was studied by flow cytometry only. RESULTS： FACS analysis indicated that EpCAM ＋ve cells were positive for CD29, CD49f, CD90, CD34, HLA class I, albumin and AFP but negative for HLA class Ⅱ （DR） and CD45. RT PCR showed that EpCAM ＋ve cells expressed liver epithelial markers （CK18）, biliary specific marker （CK19） and hepatic markers （albumin, AFP）. On immunocytochemical staining, EpCAM ＋ve cells were shown positive signals for CK18 and albumin. CONCLUSION： Our study suggests that these EpCAM ＋ve cells can be used as hepatic progenitors for cell transplantation with a minimum risk of alloreactivity and these cells may serve as a potential source for enrichment of hepatic progenitor.展开更多
Objective: To investigate a possible role of apoptosis in the pathophysiologic mechanisms of PIH (pregnancy-induced hypertension syndrome).Methods: In this study, placental samples were obtained from 16 uncomplicated ...Objective: To investigate a possible role of apoptosis in the pathophysiologic mechanisms of PIH (pregnancy-induced hypertension syndrome).Methods: In this study, placental samples were obtained from 16 uncomplicated third-trimester pregnancies and from 16 cases of PIH.We used light microscopy, electron microscopy to identify apoptosis.Light microscopy was used to quantify their incidence of apoptosis.Electron microscopy was used to confirm the occurrence of apoptosis.Results: Apoptosis has been conclusively demonstrated within human third-trimester placental tissue.Medians and interquartile ranges of normal placenta (n=16) was 0.12% (0.08%-0.19%); Medians and interquartile ranges of PIH group (n=16) was 0.37% (0.15%-0.49%).Compared to normal placentas, the incidence of apoptosis was higher in placentas from gestations complicated by PIH (P<0.05, T'-test).Conclusion: Placental apoptosis increases significantly in PIH, and it may play a role in the pathophysiologic mechanisms of this syndrome.展开更多
目的探讨孕期不同时期增重对子痫前期、妊娠期高血压、妊娠期糖尿病、小于胎龄儿(small for gestational age,SGA)和大于胎龄儿等妊娠并发症的影响。方法本研究为前瞻性纵向队列研究。于2017年1月至2019年11月在南京大学医学院附属鼓楼...目的探讨孕期不同时期增重对子痫前期、妊娠期高血压、妊娠期糖尿病、小于胎龄儿(small for gestational age,SGA)和大于胎龄儿等妊娠并发症的影响。方法本研究为前瞻性纵向队列研究。于2017年1月至2019年11月在南京大学医学院附属鼓楼医院招募孕11~13周^(+6)单胎妊娠孕妇,分别于孕19~23周^(+6)、29~34周^(+6)、35~40周^(+6)进行3次面访,现场测量孕妇的身高、体重、血压、超声指标等,并随访妊娠结局。依据早孕期体重指数将孕妇分为偏瘦组(<18.50 kg/m^(2))、正常组(18.50~23.99 kg/m^(2))和超重/肥胖组(≥24.00 kg/m^(2))。采用χ2检验和秩和检验进行组间比较。将周增重转化为Z分数,Z<-1定义为增重不足,-1≤Z≤1定义为增重适宜,Z>1定义为增重过度。利用二元logistic回归计算孕期不同时期周增重对妊娠并发症的影响。结果4143例孕妇进入队列,排除327例,最终3816例纳入分析,其中偏瘦组394例,正常组2668例,超重/肥胖组754例。中孕早期周增重过度是大于胎龄儿的危险因素(aOR=1.78,95%CI:1.31~2.42,P<0.001)。中孕晚期周增重过度是早产型子痫前期(aOR=3.00,95%CI:1.26~7.10,P=0.013)、妊娠期高血压(aOR=2.38,95%CI:1.44~3.94,P=0.001)和大于胎龄儿(aOR=1.59,95%CI:1.15~2.22,P=0.005)的危险因素。晚孕期增重过度是足月产型子痫前期(aOR=2.70,95%CI:1.61~4.54,P<0.001)和妊娠期高血压(aOR=1.84,95%CI:1.05~3.21,P=0.033)的危险因素。晚孕期增重不足是SGA的危险因素(aOR=1.58,95%CI:1.01~2.48,P=0.045),是足月产型子痫前期的保护因素(aOR=0.37,95%CI:0.14~0.97,P=0.041)。中孕早期周增重不足和过度对妊娠期糖尿病的发生无影响(P值均>0.05)。结论孕期不同时期增重对妊娠并发症的影响不同。适当放宽中孕早期增重,相对严格控制中孕晚期和晚孕期增重,可能是既有利于预防早产型子痫前期又不增加SGA发生率的合理选择。展开更多
在美国,儿童心脏缺陷能在出生前被发现的不超过1/10。波兰Oregon卫生科学大学的心脏病专家Mary Jo Rice在一次由美国心脏协会主办的会议上说道:如果妊娠妇女在妊娠头三个月以后作超声检查,那么发现此缺陷的机会将大大增加,随之患先天性...在美国,儿童心脏缺陷能在出生前被发现的不超过1/10。波兰Oregon卫生科学大学的心脏病专家Mary Jo Rice在一次由美国心脏协会主办的会议上说道:如果妊娠妇女在妊娠头三个月以后作超声检查,那么发现此缺陷的机会将大大增加,随之患先天性心脏病的小孩的生存率也会提高。在妊娠头三月的超声检查主要用于预测产期和检查胎儿的一般发育状况,但它不能得到微小胎心的清晰图象。展开更多
基金Council of Scientific and Industrial Research Network Grant CMM002ICMR Grant (GAP 0215)
文摘AIM： To enrich hepatic progenitors using epithelial cell adhesion molecule （EpCAM） as a marker from human fetal liver and investigate the expression of human leukocyte antigen （HLA） and their markers associated with hepatic progenitor cells. METHODS： EpCAM ＋ve cells were isolated using magnetic cell sorting （MACS） from human fetuses （n = 10） at 15-25 wk gestation. Expression of markers for hepatic progenitors such as albumin, alpha-fetoprotein （AFP）, CD29 （integrin ~1）, CD49f （integrin c^6） and CD90 （Thy 1） was studied by using flow cytometry, immunocytochemistry and RT-PCR; HLA class Ⅰ （A, B, C） and class Ⅱ （DR） expression was studied by flow cytometry only. RESULTS： FACS analysis indicated that EpCAM ＋ve cells were positive for CD29, CD49f, CD90, CD34, HLA class I, albumin and AFP but negative for HLA class Ⅱ （DR） and CD45. RT PCR showed that EpCAM ＋ve cells expressed liver epithelial markers （CK18）, biliary specific marker （CK19） and hepatic markers （albumin, AFP）. On immunocytochemical staining, EpCAM ＋ve cells were shown positive signals for CK18 and albumin. CONCLUSION： Our study suggests that these EpCAM ＋ve cells can be used as hepatic progenitors for cell transplantation with a minimum risk of alloreactivity and these cells may serve as a potential source for enrichment of hepatic progenitor.
文摘Objective: To investigate a possible role of apoptosis in the pathophysiologic mechanisms of PIH (pregnancy-induced hypertension syndrome).Methods: In this study, placental samples were obtained from 16 uncomplicated third-trimester pregnancies and from 16 cases of PIH.We used light microscopy, electron microscopy to identify apoptosis.Light microscopy was used to quantify their incidence of apoptosis.Electron microscopy was used to confirm the occurrence of apoptosis.Results: Apoptosis has been conclusively demonstrated within human third-trimester placental tissue.Medians and interquartile ranges of normal placenta (n=16) was 0.12% (0.08%-0.19%); Medians and interquartile ranges of PIH group (n=16) was 0.37% (0.15%-0.49%).Compared to normal placentas, the incidence of apoptosis was higher in placentas from gestations complicated by PIH (P<0.05, T'-test).Conclusion: Placental apoptosis increases significantly in PIH, and it may play a role in the pathophysiologic mechanisms of this syndrome.
文摘在美国,儿童心脏缺陷能在出生前被发现的不超过1/10。波兰Oregon卫生科学大学的心脏病专家Mary Jo Rice在一次由美国心脏协会主办的会议上说道:如果妊娠妇女在妊娠头三个月以后作超声检查,那么发现此缺陷的机会将大大增加,随之患先天性心脏病的小孩的生存率也会提高。在妊娠头三月的超声检查主要用于预测产期和检查胎儿的一般发育状况,但它不能得到微小胎心的清晰图象。
