To improve antagonistic metabolites production of Bacillus subtilis strain BS501a, physical parameters of fermentation and metal inorganic salts in medium, namely initial pH value, culture temperature, fermentation ti...To improve antagonistic metabolites production of Bacillus subtilis strain BS501a, physical parameters of fermentation and metal inorganic salts in medium, namely initial pH value, culture temperature, fermentation time, concentrations of CaC12, FeSO4, ZnSO4, MnSO4 and MgSO4, were optimized using one-factor-at-a-time and orthogonal tests. The results show that the optimal physical parameters of fermentation are an initial pH of 7.0, a culture temperature of 30 ~C, and a fermentation time of 48 h. The optimal concentrations of metal inorganic salts in basal medium are 10.2 mmol/L CaCl2, 0.4 mmol/L FeSO4, 3.5 mmol/L ZnSO4, 0.6 mmol/L MnSO4 and 2.0 mmol/L MgSO4. Among the metal inorganic salts, MgSO4 and MnSO4 play important roles in the improvement of the antagonistic metabolites production of B. subtilis strain BS501a; especially, MgSO4 contributes a highly significant effect. The average diameter of inhibition zone of the BS501a filtered fermentation supernatant （FFS） cultured in the optimal fermentation conditions against Magnaporthe grisea DWBJ329 reaches 71.4 mm, and there is 2.4-fold increase in antifungal activity as compared with 21.2 mm under the pre-optimized conditions.展开更多
[ Objective] The aim was to study the fermentation technology of monascus pigment of monascus strains JF-02. [ Method] Single factor experiment was carried out to study the influence of temperature, initial pH, cultur...[ Objective] The aim was to study the fermentation technology of monascus pigment of monascus strains JF-02. [ Method] Single factor experiment was carried out to study the influence of temperature, initial pH, culture time, different agricultural byproduct, and nitrogen source on monascus pigment in fermentation solution. Meanwhile, orthogonal experiment was conducted to get the optimal culture medium and cultivation condition. [ Resultl The optimal gene in the pigment of monascus pigment was 200 g/L of rice, 30 g/L of sweet potato powder, 10 g/L of glucose, 15 g/L of monosodium glutamate, 0.1% of zinc sulfate, and 0.1% of magnesium sulfate. The optimal culture condition was 30 ℃ and initial pH was 6.0. Fermentation time was 72 h, but when 24-L fermentation pot was used, culture time can last to 84 h. [ Condusion] The study provided theoretical basis for the development and application of monascus strains.展开更多
This paper, using the dataset of BBFs （bursty bulk flows） observed by two Cluster satellites C1 and C4, studies the difference between onset times of BBFs observed by C1 and C4. It is found that the onset time diffe...This paper, using the dataset of BBFs （bursty bulk flows） observed by two Cluster satellites C1 and C4, studies the difference between onset times of BBFs observed by C1 and C4. It is found that the onset time differences of most of BBFs observed by Cl and C4 are smaller than 60 s. The average onset time difference of BBFs of CI and C4 is 68.5 s. The probabilities of onset time difference of BBFs of C1 and C4 larger than 30, 60, 90 and 120 s are respectively 55%, 35%, 27% and 23%. The largest onset time difference of BBFs of C1 and C4 decreases with the increase of earthward component of maximum velocities of BBFs. The onset time difference of BBFs of C1 and C4 results from the velocity inhomogeneity inside the flow channel of BBF, which may be produced in propagation path and/or in source region of BBFs. Such a wide range of onset time difference of BBFs suggests that the velocity inhomogeneity inside the flow channel of BBF is various. These results are very important to the current study of substorm research based on THEMIS data because they indicate that it is impossible to determine the onset time of BBF with a single satellite.展开更多
基金Project(2010A210003) supported by Henan Province Natural Sciences Research PlanProject(0910SGYS34370-2) supported by Zhengzhou City Science and Technology Research PlanProject supported by the Youth Backbone Teacher of Universities in Henan Province Grants Plan
文摘To improve antagonistic metabolites production of Bacillus subtilis strain BS501a, physical parameters of fermentation and metal inorganic salts in medium, namely initial pH value, culture temperature, fermentation time, concentrations of CaC12, FeSO4, ZnSO4, MnSO4 and MgSO4, were optimized using one-factor-at-a-time and orthogonal tests. The results show that the optimal physical parameters of fermentation are an initial pH of 7.0, a culture temperature of 30 ~C, and a fermentation time of 48 h. The optimal concentrations of metal inorganic salts in basal medium are 10.2 mmol/L CaCl2, 0.4 mmol/L FeSO4, 3.5 mmol/L ZnSO4, 0.6 mmol/L MnSO4 and 2.0 mmol/L MgSO4. Among the metal inorganic salts, MgSO4 and MnSO4 play important roles in the improvement of the antagonistic metabolites production of B. subtilis strain BS501a; especially, MgSO4 contributes a highly significant effect. The average diameter of inhibition zone of the BS501a filtered fermentation supernatant （FFS） cultured in the optimal fermentation conditions against Magnaporthe grisea DWBJ329 reaches 71.4 mm, and there is 2.4-fold increase in antifungal activity as compared with 21.2 mm under the pre-optimized conditions.
基金Supported by Zhenjiang Science and Technology Plan Foundation in Jiangsu Province(NY2008047)
文摘[ Objective] The aim was to study the fermentation technology of monascus pigment of monascus strains JF-02. [ Method] Single factor experiment was carried out to study the influence of temperature, initial pH, culture time, different agricultural byproduct, and nitrogen source on monascus pigment in fermentation solution. Meanwhile, orthogonal experiment was conducted to get the optimal culture medium and cultivation condition. [ Resultl The optimal gene in the pigment of monascus pigment was 200 g/L of rice, 30 g/L of sweet potato powder, 10 g/L of glucose, 15 g/L of monosodium glutamate, 0.1% of zinc sulfate, and 0.1% of magnesium sulfate. The optimal culture condition was 30 ℃ and initial pH was 6.0. Fermentation time was 72 h, but when 24-L fermentation pot was used, culture time can last to 84 h. [ Condusion] The study provided theoretical basis for the development and application of monascus strains.
基金supported by the National Natural Science Foundation of China (Grant Nos. 40931054 and 41174141)National Basic Research Program of China ("973" Program) (Grant No. 2011CB811404)
文摘This paper, using the dataset of BBFs （bursty bulk flows） observed by two Cluster satellites C1 and C4, studies the difference between onset times of BBFs observed by C1 and C4. It is found that the onset time differences of most of BBFs observed by Cl and C4 are smaller than 60 s. The average onset time difference of BBFs of CI and C4 is 68.5 s. The probabilities of onset time difference of BBFs of C1 and C4 larger than 30, 60, 90 and 120 s are respectively 55%, 35%, 27% and 23%. The largest onset time difference of BBFs of C1 and C4 decreases with the increase of earthward component of maximum velocities of BBFs. The onset time difference of BBFs of C1 and C4 results from the velocity inhomogeneity inside the flow channel of BBF, which may be produced in propagation path and/or in source region of BBFs. Such a wide range of onset time difference of BBFs suggests that the velocity inhomogeneity inside the flow channel of BBF is various. These results are very important to the current study of substorm research based on THEMIS data because they indicate that it is impossible to determine the onset time of BBF with a single satellite.
