姬松茸高产栽培技术关键

我所于１９９４年从日本引进姬松茸８号菌种，通过小面积试种取得了成功。１９９５年后在各地进行较大面积推广，均普遍获得了良好经济效益。在短短４年中，我们对姬松茸的栽培工艺、原料配方、原料增温发酵、技术管理等进行反复试验，不断总结，积累了较丰富的栽培技术，...

姬松茸多糖对肥大细胞脱颗粒的影响

目的:观察姬松茸多糖(Agaricus blazei polysaccharides,ABP)的抗过敏作用并探讨其可能的作用机制。方法:通过IgE诱导大鼠被动皮肤过敏反应(PCA)实验,用比色法测定姬松茸多糖在体内对肥大细胞影响。体外实验观察姬松茸多糖对IgE诱导组胺释放、肿瘤坏死因子-α、白细胞介素6及p38MAPK的影响。结果:体内实验显示,姬松茸多糖高(400mg/kg)、中(200mg/kg)剂量明显抑制大鼠PCA。姬松茸多糖高(100μg/L)、中(50μg/L)剂量了肥大细胞脱颗粒、组胺的释放、以及肿瘤坏死因子-α、白细胞介素6的释放以及p38MAPK、NF-κB p65蛋白的表达。结论:姬松茸多糖抗过敏作用部分可能与抑制p38MAPK、NF-κB p65信号通路有关。

新型试管花卉姬松高效再生体系的研究

以新型试管花卉多肉类植物姬松(Crassula clavata N.E.Brown)为材料进行高效再生体系的研究。结果表明:以姬松茎尖为外植体的初代培养中,MS培养基添加IBA并未诱导出根系生成,而是直接诱导出丛生芽,在MS+IBA0.3 mg·L-1培养基上,由叶片表面诱导出的丛生芽质量最佳;在继代培养中,最佳培养基为MS+NAA 0.1 mg·L-1+6-BA 0.5 mg·L-1,诱导产生大量胚性愈伤组织,且表面有芽体分化;在彩色培养中,在1/2MS中添加0.25 mg·L-1的食用色素亮蓝,观赏效果佳且对植株生长无毒害。

Transcriptome Sequencing and Analysis of Agaricus blazei Mycelia Under Cadmium Stress

To explore the mechanism of Agaricus blazei Murrill enrichment of the heavy metal cadmium,we employed Illumina high-throughput sequencing to analyze the transcriptomes of A.blazei mycelia treated with and without exogenous cadmium addition,and then the differentially expressed genes(DEGs)of the strains with high and low cadmium enrichment between the control and cadmium treatment were screened out.The results showed that the DEGs were mainly involved in steroid biosynthesis,antibiotic biosynthesis,protein processing in endoplasmic reticulum,glutathione metabolism and other pathways.Carbon metabolism and glutathione metabolism may play an important role in the response of A.blazei mycelium to cadmium stress.