Clinico-pathological study of experimental Eimeria species infection （coccidiosis） such as E. magna, E. intestinalis, E. irresidua, E. media and E. perforans in rabbits was conducted with dose inocula of 1 lac sporu...Clinico-pathological study of experimental Eimeria species infection （coccidiosis） such as E. magna, E. intestinalis, E. irresidua, E. media and E. perforans in rabbits was conducted with dose inocula of 1 lac sporulated oocysts. These rabbits were divided into two groups each consisting of 12 rabbits. Two rabbits of infected untreated group died of severe disease on the 27^th ＆ 28^th day post infection. The intestine of dead animals showed elevated lesions in the middle half portion with congestion and destruction of villar epithelium.展开更多
Telocytes （TCs） are a novel type of interstitial cells that are thought to be involved in tissue regeneration and repair. However, the possible roles of TCs in vascular diseases remain unclear. In this study, we use...Telocytes （TCs） are a novel type of interstitial cells that are thought to be involved in tissue regeneration and repair. However, the possible roles of TCs in vascular diseases remain unclear. In this study, we used a rat model of carotid artery balloon injury （CABI） to study the changes and potential roles of vascular TCs after vascular injury. Transmission electron microscopy （TEM） and CD34/vimentin immunolabeling were used to identify and quantify TCs in normal and injured carotid arteries. Quantita- tive immunofluorescence analysis revealed that, compared with the sham group, the number of TCs in the CAB1 group in- creased from 7.2＋1.0 to an average of 20.4＋1.8 per l-ram2 vascular area. The expression level of miR-24 in TCs was three times higher than in vascular smooth muscle cells （VSMCs）. The percentage of VSMCs in S phase and G2/M phase increased by approximately 5% when VSMCs were incubated with the supematant of TCs. The antagomir of miR-24 in TCs reduced the ratio of VSMCs in S ohase and G2/M phase. This study illuminates the function of TCs in the proliferation of VSMCs.展开更多
文摘Clinico-pathological study of experimental Eimeria species infection （coccidiosis） such as E. magna, E. intestinalis, E. irresidua, E. media and E. perforans in rabbits was conducted with dose inocula of 1 lac sporulated oocysts. These rabbits were divided into two groups each consisting of 12 rabbits. Two rabbits of infected untreated group died of severe disease on the 27^th ＆ 28^th day post infection. The intestine of dead animals showed elevated lesions in the middle half portion with congestion and destruction of villar epithelium.
基金supported by the National Natural Science Foundation of China (91339105)the Beijing Natural Science Foundation (7142165)the Science and Technology Commission of Beijing Municipality (z141100000214006)
文摘Telocytes （TCs） are a novel type of interstitial cells that are thought to be involved in tissue regeneration and repair. However, the possible roles of TCs in vascular diseases remain unclear. In this study, we used a rat model of carotid artery balloon injury （CABI） to study the changes and potential roles of vascular TCs after vascular injury. Transmission electron microscopy （TEM） and CD34/vimentin immunolabeling were used to identify and quantify TCs in normal and injured carotid arteries. Quantita- tive immunofluorescence analysis revealed that, compared with the sham group, the number of TCs in the CAB1 group in- creased from 7.2＋1.0 to an average of 20.4＋1.8 per l-ram2 vascular area. The expression level of miR-24 in TCs was three times higher than in vascular smooth muscle cells （VSMCs）. The percentage of VSMCs in S phase and G2/M phase increased by approximately 5% when VSMCs were incubated with the supematant of TCs. The antagomir of miR-24 in TCs reduced the ratio of VSMCs in S ohase and G2/M phase. This study illuminates the function of TCs in the proliferation of VSMCs.
