The human gene ATP 13A2 has been proposed to code for an ATP powered ion transporter of the PsB subfamily. Mutations of the human gene ATP1 3A2 were found to underlie an autosomal recessive form of early-onset parkins...The human gene ATP 13A2 has been proposed to code for an ATP powered ion transporter of the PsB subfamily. Mutations of the human gene ATP1 3A2 were found to underlie an autosomal recessive form of early-onset parkinsonism （PD） with pyramidal degeneration and dementia. The ion transported by the ATP13A2 pump is not known, but several studies have shown that the Ps-ATPases influence the homeostasis of intracellular Ca2＋, and thus it has been suggested that they transport Ca2＋. In order to evaluate this possibility Chinese hamster ovary （CHO） cells stably expressing the human ATP13A2 protein have been obtained and the Ca2＋ transport activity of ATP 13A2 was assessed by measuring the ATP-dependent uptake of Ca2＋ into microsomal vesicles. As a positive control vesicles containing the human plasma membrane Ca2＋ pump （PMCA） were used. No significant differences were found between vesicles containing the ATP 13A2 protein and the control. Moreover, Ca2＋ was unable to induce the formation of the P-ATPase acylphosphate intermediate in vesicles containing the expressed ATPl3A2. These results favor the idea that the ATPI3A2 does not transport Ca2＋.展开更多
β-amyloid (Aβ) and copper play important roles in the pathogenesis of Alzheimer’s disease (AD).However,the behavioral correlativity and molecular mechanisms of Aβ and copper toxicity have been investigated less of...β-amyloid (Aβ) and copper play important roles in the pathogenesis of Alzheimer’s disease (AD).However,the behavioral correlativity and molecular mechanisms of Aβ and copper toxicity have been investigated less often.In the present study,we investigated the interaction and toxicity of Aβ1-42 and copper in the Aβ1-42 transgenic Caenorhabditis elegans worm model CL2006.Our data show that the paralysis behavior of CL2006 worms significantly deteriorated after exposure to 10-3 mol L-1 copper ions.However,the paralysis behavior was dramatically attenuated with exposure to 10-4 mol L-1 copper ions.The exogenous copper treatment also partially changed the homeostatic balance of zinc,manganese,and iron.Our data suggest that the accumulation of reactive oxygen species (ROS) was responsible for the paralysis induced by Aβ and copper in CL2006.The ROS generation induced by Aβ and copper appear to be through sod-1,prdx-2,skn-1,hsp-60 and hsp-16.2 genes.展开更多
Terminalia chebula(family:Combretaceae)is widely used in the traditional medicine of India and Iran to treat diseases that include dementia,constipation,and diabetes.This tree is known in Iranian traditional medicine(...Terminalia chebula(family:Combretaceae)is widely used in the traditional medicine of India and Iran to treat diseases that include dementia,constipation,and diabetes.This tree is known in Iranian traditional medicine(ITM)as halileh or halilaj and the fruit is used to develop treatments.It is described in ITM as an astringent that has a"cold"and"dry"temperament.References to the medicinal properties of Terminalia chebula were collected from important ITM sources and from modern medical databases(Pub Med,Scirus,Science Direct,and Scopus).The medicinal properties described for thistree in ITM were compared with those reported in studies of modern phytotherapy.The results confirm that the tree referred to as halileh in traditional books is the Terminalia chebula used in present-day studies.Treatments that have not been evaluated in modern phytotherapy but have been traditionally treated with Terminalia chebula include fever,and psychological and psychiatric issues.This article confirms the medicinal uses of Terminalia chebula.展开更多
文摘The human gene ATP 13A2 has been proposed to code for an ATP powered ion transporter of the PsB subfamily. Mutations of the human gene ATP1 3A2 were found to underlie an autosomal recessive form of early-onset parkinsonism （PD） with pyramidal degeneration and dementia. The ion transported by the ATP13A2 pump is not known, but several studies have shown that the Ps-ATPases influence the homeostasis of intracellular Ca2＋, and thus it has been suggested that they transport Ca2＋. In order to evaluate this possibility Chinese hamster ovary （CHO） cells stably expressing the human ATP13A2 protein have been obtained and the Ca2＋ transport activity of ATP 13A2 was assessed by measuring the ATP-dependent uptake of Ca2＋ into microsomal vesicles. As a positive control vesicles containing the human plasma membrane Ca2＋ pump （PMCA） were used. No significant differences were found between vesicles containing the ATP 13A2 protein and the control. Moreover, Ca2＋ was unable to induce the formation of the P-ATPase acylphosphate intermediate in vesicles containing the expressed ATPl3A2. These results favor the idea that the ATPI3A2 does not transport Ca2＋.
基金supported by the National Natural Science Foundation of China (Grant No. 30870578)the National Basic Research Program of China (Grant No. 2006CB500700)funded by the US National Institutes of Health for providing nematode strains used in this work
文摘β-amyloid (Aβ) and copper play important roles in the pathogenesis of Alzheimer’s disease (AD).However,the behavioral correlativity and molecular mechanisms of Aβ and copper toxicity have been investigated less often.In the present study,we investigated the interaction and toxicity of Aβ1-42 and copper in the Aβ1-42 transgenic Caenorhabditis elegans worm model CL2006.Our data show that the paralysis behavior of CL2006 worms significantly deteriorated after exposure to 10-3 mol L-1 copper ions.However,the paralysis behavior was dramatically attenuated with exposure to 10-4 mol L-1 copper ions.The exogenous copper treatment also partially changed the homeostatic balance of zinc,manganese,and iron.Our data suggest that the accumulation of reactive oxygen species (ROS) was responsible for the paralysis induced by Aβ and copper in CL2006.The ROS generation induced by Aβ and copper appear to be through sod-1,prdx-2,skn-1,hsp-60 and hsp-16.2 genes.
文摘Terminalia chebula(family:Combretaceae)is widely used in the traditional medicine of India and Iran to treat diseases that include dementia,constipation,and diabetes.This tree is known in Iranian traditional medicine(ITM)as halileh or halilaj and the fruit is used to develop treatments.It is described in ITM as an astringent that has a"cold"and"dry"temperament.References to the medicinal properties of Terminalia chebula were collected from important ITM sources and from modern medical databases(Pub Med,Scirus,Science Direct,and Scopus).The medicinal properties described for thistree in ITM were compared with those reported in studies of modern phytotherapy.The results confirm that the tree referred to as halileh in traditional books is the Terminalia chebula used in present-day studies.Treatments that have not been evaluated in modern phytotherapy but have been traditionally treated with Terminalia chebula include fever,and psychological and psychiatric issues.This article confirms the medicinal uses of Terminalia chebula.
