Histological Study on Soybean Somatic Embryogenesis

Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryogenesis system of soybean was used to conduct genetic transformation of soybean because of its several advantages such as higher transformational efficiency, beetter synchronism and fewer plant chimeras among transgenic plants. After infected with agrobacterium tumefaciens,the initiation, differentiation and development of young cotyledon embryogenic cell of soybean which was cultured on selective culture medium with kanamycin were investigated through histological study. The result showed that somatic embryo was differentiated in non-bud differentiation way. The embryogenic cells were differentiated from epidermis of explant or cells in 1 layer or 2 layers, with the division of embryogenic cells and degradation and disorganization of surrounding cells, the embryogenic cells would form embryoid with analogous suspensor structure. Later, globular embryoid would extrude from epidermis then developed into heart-shape embryo. The experiment was expected to provide theoretical reference for the construction of high transformational system of using plant somatic embryogenesis induced by young cotyledon of soybean.

锅炉KKS编码

介绍KKS编码的由来、KKS编码国际标准 ,阐述了锅炉KKS编码的方法。

Clinical Research on the Changes of Plasma TFPI and uPA System in Malignant Tumor

Objective: To investigate the expression levels and significance of TFPI, uPA, uPAR and PAI in malignant patients. Methods: The levels of TFPI, uPA and uPAR were measured by using ELISA and the level of PAI was determined by method of chromogenic substrates in 44 patients with malignant solid tumors (group A1) and 30 patients with acute leukemia (AL, group A2). Results: The levels of TFPI, uPA, and uPAR in group A1 were higher than those in normal control group (group B). TFPI, uPAR levels in group A2 were higher than those in group B, while the level of PAI in group A2 was lower than that in group B. Among the groups, TFPI was increased in the combined infection group; PAI decreased in the hemorrhage group; TFPI, uPA, uPAR and PAI increased in relapsing and metastasis group; TFPI decreased in one-week dead group, while uPA and uPAR increased. Conclusion: The patients with malignant solid tumor and AL had different anticoagulation or fibrinolysis states. TFPI, uPA, Upar and PAI can be used to evaluate the disease condition and the prognosis.

A hybrid neural network system for prediction and recognition of promoter regions in human genome

This paper proposes a high specificity and sensitivity algorithm called PromPredictor for recognizing promoter regions in the human genome. PromPredictor extracts compositional features and CpG islands information from genomic sequence,feeding these features as input for a hybrid neural network system (HNN) and then applies the HNN for prediction. It combines a novel promoter recognition model, coding theory, feature selection and dimensionality reduction with machine learning algorithm.Evaluation on Human chromosome 22 was ～66% in sensitivity and ～48% in specificity. Comparison with two other systems revealed that our method had superior sensitivity and specificity in predicting promoter regions. PromPredictor is written in MATLAB and requires Matlab to run. PromPredictor is freely available at http://www.whtelecom.com/Prompredictor.htm.

Kondo Effect Versus Magnetic Coupling in Indirectly Coupled Double Quantum Dots

We theoretically investigate a device consisting of two quantum dots （QDs） side-coupled to a quantum wire which has many physical ingredients of an artificial heavy fermion system. An extra parameter, the distance L between the two QDs, is introduced and it plays an important role on the competition of the Kondo temperature and magnetic coupling. Three different phases are found： antiferromagnetic phase, Kondo phase with spin S = 1/2, and Kondo phase with S = 1, depending on the distance L, the magnetic properties are qualitatively different for different phases： conductance tends to the unitary value 2e2 /h; for the S ： the distance. coupling, and the Kondo temperature. Quantum transport for the S = 1 Kondo and the antiferromagnetic phases, the 1/2 Kondo phase the conductance is strongly dependent onthe distance.

A planar parallel manipulator based novel MEMS device bonding system

A novel MEMS device boning system is presented. Aiming at the high velocity, high precision and high flexibility requirements, a novel manipulator of planar parallel structure is developed to substitute ordinary X-Y table. In addition, the machine vision is implemented to improve the system＇ s flexibility. The initial angular positions of the joints are estimated by the extended Kalman filter algorithm. As a resuh, the manipulator＇s absolute locating accuracy in its workspace is guaranteed indirectly. For any MEMS device, the bonding system itself can be used as measurement equipment to create the device＇ s geometry model, which is the base to do off-line programming. A quite ideal trade-off between the system＇ s flexibility and efficiency is got. Finally, some verified motion specification of the manipulator, the bonding experimental results and the verified qualities of the bonded devices are provided.

Bell's Inequality for a System Composed of Particles with Different Spins

For two particles with different spins, we derive the Bell＇s inequality. The inequality is investigated for two systems combining spin-1 and spin-1/; spin-1/2 and spin-3/2. We show that for these states Bell＇s inequality is violated.

Blended Learning and Applying New Tools and Services of E-Learning Support

The strengths and weaknesses of different online technologies and learning methods have been examined by combining the blended learning program with practical work on institutional e-learning projects. There are differences between the traditional e-learning system and the new E-Learning 2.0 training. At present time, the new technologies provided by Web 2.0 better correspond to the needs of both students and teaching staff. The new Web 2.0 allows the ＂promoting of individual creativity＂, together with the ＂use of collective intelligence＂. A comparison of the two development stages of the e-learning tools has been completed and the advantages of E-Learning 2.0 are shown. The main components of Web 2.0 are provided and the use ofwiki as an up-to-date collaboration tool for on-line teamwork is shown.

Marked Advance in Widths and Heights of Harmonic Plateau

We propose a scheme to simultaneously widen and heighten the high-order harmonic plateau on a large scale. More specifically, by adopting a united two-atom system with a suitable inter-nuclear separation instead of a single-atom, the harmonic plateau is widened from Ip ＋ 3.2Up to Ip ＋8.5Up; further, by adopting the combined pulse, the extended plateau （harmonics near Ip＋ 5.6Up） is selectively heightened in excess of 4 orders of magnitude compared with the case of the low-frequency pulse alone. By means of the wavelet transform for the induced dipole of these harmonics, a single x-ray pulse as short as 210 asec is achieved.

Analysis and design of excitation system in homopolar inductor machine

The excitation system of the homopolar inductor machine(HIM)is analyzed and designed to establish the design approach and evaluation criteria of the excitation system.The finite element method is used to calculate the three-dimensional electromagnetic field in the HIM,and the distribution and characteristics of the magnetic field are described.The analytical method is applied to investigate the design process of the excitation winding.The ratio of the axial length of the armature winding to the excitation winding and the ratio of the axial length of the working air-gap to the non-working air-gap are investigated by the numerical calculation method.A prototype HIM is designed and manufactured,and some experiments are implemented to verify the correctness of the theoretical analysis and numerical calculation results.The research results show that the established design method of the excitation winding is practical and feasible.Under the conditions of constant excitation magnetomotive force or constant excitation power,the optimum range of the axial length ratio of the armature winding and the excitation winding is 0.45 to 0.5.The optimal axial length of the non-working air-gap can be determined by the ratio of the stator inner diameter and pole-pairs.

The Applicability of the Density Rule of Pathwardhan and Kumer and the Rule Based on Linear Isopiestic Relation

The applicability of the density rule of Pathwardhan and Kumer and the rule based on the linear isopiestic relation is studied by comparison with experimental density data in the literature. Predicted and measured values for 18 electrolyte mixtures are compared. The two rules are good for mixtures with and without common ions, including those containing associating ions. The deviations of the rule based on the linear isopiestic relation are slightly higher for the mixtures involving very strong ion complexes, but the predictions are still quite satisfactory.The density rule of Pathwardhan and Kumer is more accurate for these mixtures. However, it is not applicable for mixtures containing non-electrolytes. The rule based on the linear isopiestic relation is extended to mixtures involving non-electrolytes. The predictions for the mixtures containing both electrolytes and non-electrolytes and the non-electrolyte mixtures are accurate. All these results indicate that this rule is a widely applicable approach.

The latest progress in research of plastics processing technology

According to the great demand for the＂ green＂ plastics processing technology of the low energy consumption, high efficiency and environmental protection in plastics industry, the plastics processing method and technology based on the elongation rheology, with continuing evolution and innovation of the plastics plasticating and conveying method, are presented and researched on the basis of the plastics dynamic processing method arid equipment, and the plastics plasticating and conveying process in the vane extrusion system, the technical characteristics and the applications of vane plasticating and conveying technology are discussed. The research results show that compared with the conventional processing equipment, this new technology and equipment shows many outstanding advantages, such as shortening the thermo-meehanical history of the plastics processing by more than 50 % , reducing the energy consumption by 30 % or so, improving the mixing and blending effects, improving the quality of the products and the adaptability to materials, etc. , and it is found that the new technology and equipment has special superiority in the fields of the processing for material systems, such as the multiphase and multicomponent composite materials, the shear heat sensitive macromolecular materials, etc.

Self-organization of Atoms into Nanosystems

The mechanism of addition of separate atoms to a growing center is considered with application of model of a pair interpenetration of atoms. Features of geometrical model are related with the electronic structure of atoms and the Pauli＇s exclusion principle. The forces providing self-organization of atoms in the bulk of a condensed substance are shown. The calculated interatomic distances in graphite and alloys of gold with silver coincide with those known from experiments with accuracy of 0.1%.

Molecular Partners of Escherichia coil Transcriptional Modulator AidB

The AidB protein is involved in the adaptive response to DNA alkylation damages in Escherichia coli. Functional proteomic experiments were designed to elucidate AidB biological functions in the presence and in the absence of methyl methanesulfonate as methylating agent. Several proteins were identified in both conditions and according to their reported biological activities, the inter-actors were grouped into three different functional categories： stress response, energetic metabolic pathways and nucleic acid metabolism. Particularly, the interaction between AidB and UvrA, a member of the UvrABCD nucleotide excision system, suggested a new interesting putative role for AidB.

Integrability Aspects and Soliton Solutions for a System Describing Ultrashort Pulse Propagation in an Inhomogeneous Multi-Component Medium

For the propagation of the ultrashort pulses in an inhomogeneous multi-component nonlinear medium, a system of coupled equations is analytically studied in this paper. Painleve analysis shows that this system admits the Painleve property under some constraints. By means of the Ablowitz-Kaup-Newell-Segur procedure, the Lax pair of this system is derived, and the Darboux transformation （DT） is constructed with the help of the obtained Lax pair. With symbolic computation, the soliton solutions are obtained by virtue of the DT algorithm. Figures are plotted to illustrate the dynamical features of the soliton solutions. Characteristics of the solitons propagating in an inhomogeneous multi-component nonlinear medium are discussed： （i） Propagation of one soliton and two-peak soliton; （ii） Elastic interactions of the parabolic two solitons; （iii） Overlap two head-on solitons and two head-on two-peak solitons; （v） Two （vi） Decomposition phenomenon of one soliton into two solitons. phenomenon between two solitons; （iv） Collision of different types of interactions of the three solitons; ultrashort-pulse propagation in the inhomogeneous multi-component The results might be useful in the study on the nonlinear media.

Asymptotic Structure in the Classically Forbidden Region of the Hooke’s Atoms

The two-electron Hooke's atom - a quantum mechanical system with two electrons bound in a harmonic potential - is well known for its exact analytical properties at certain oscillator strengths. The Hooke's atoms with more than two electrons offer more scope for valuable practical applications. In this work, we study the asymptotic structure of these Hooke's atoms in the classically forbidden region. The leading-order term of the long-range expression for the KS exchange-correlation potential v xc (r) is shown to be-1/r. The second and third higher order terms are also exactly obtained. Various components of v xc (r) are also studied. It is shown that the leading term of O(1/r) in vxc (r) is due to the pure Pauli correlation, while the leading contribution of the Coulomb correlation is of O(1/r3 ). Neither of them makes contribution to the term of O(1/r2 ), which is shown to be solely due to the kinetic correlation effect. Results for the two-electron Hooke's atom were obtained before in the literature. Our results reduce to those of the two-electron Hooke's atom as a special case.

Template-module assembly to prepare low-molecular-weight gene transport system with enhanced transmembrane capability

Based on specific host-guest interactions between amine-modified [3-cyclodextrin （CD-TAEA） and functional adamantane （AD） derivatives, a module-template strategy has been proposed for the construction of low-molecular-weight cationic assem- blies for gene transport. This strategy offers great flexibility in terms of the introduction of mono- or multi-functionality by the inclusion of one or more adamantane-based modules with the desired functionalities. As proof of concept, phenylboronic acid （PB） containing adamantane （PB-AD） was used as a model module in the hope of offering enhanced cytosolic delivery in con- sideration of the special affinity of PB groups with cell membranes. The physicochemical properties of the complexes formed with plasmid DNA, such as particle size, zeta potential and morphology were investigated. Confocal laser scanning microsco- py and flow cytometry experiments demonstrated the important contribution of the functional PB-AD module to the consider- ably enhanced intracellular internalization and uptake by cellular nuclei. Compared to the parent CD-TAEA, PB-AD/CD- TAEA assemblies mediated higher transfection rates, which were even comparable to that of PEI25K. In addition, PB-AD/CD- TAEA displayed much lower cytotoxicity than PEI25K in both 293T and HeLa cell lines. The encouraging results suggest that CD-TAEA can be developed as a powerful template capable of readily accommodating various AD-based modules giving versatile functionalities for improved transfection.

Optimized Ewald sum for electrostatics in molecular self-assembly systems at interfaces

We extend the recent formulation of the Ewald sum for electrostatics in a two-dimensionally periodic three-dimensional multi- atom layer or two-dimensional single-atom layer system with a rectangular periodic boundary condition （J Chem Theory, Comput, 2014, 10： 534-542） to that with a parallelogrammic periodic boundary condition in general. Following the discussion of an efficient implementation of the formula, we suggest a simple setup of parameters using a relatively smaller screening factor and the associated larger real space cutoff distance to reach an optimized algorithm of an order N computational cost. The connection between the previous application of the Ewald sum to ionic crystal systems and the future application to mo- lecular self-assembly or disassembly systems on solid surfaces or at liquid-liquid interfaces ate illustrated to demonstrate the applicability of the present work to simulate the self-assembly process and to produce dynamical, structural and thermody- namic properties of experimental self-assembly systems of interest.

The influence of agglomerates on the densification and microstructural evolution in sintering of a multi-particle system

Effects of agglomerates on the densification behavior and microstructural evolution during solid-state sintering of a cube of copper particles have been studied with discrete element method (DEM).It is found that the densification of the sintering system decreases as the volume fraction of agglomerates increases.At a given volume fraction of agglomerates,the smaller the size of agglomerates,the poorer the densification and more inhomogeneous the compact is.The morphology and distribution of agglomerates have negligible effects on the densification,especially for the case with a low volume fraction of agglomerates.Agglomerates with a smaller average coordination number would have more restriction on the densification of sintering bodies.To our best knowledge,it is the first time to study the effect of agglomerates on sintering behavior using DEM.This study should be useful for further investigations of the effect of various inhomogeneities of microstructure on the complex sintering process by DEM.

Multi-solitons for a generalized Davey-Stewartson system

This paper studies multi-solitons of non-integrable generalized Davey-Stewartson system in the elliptic-elliptic case. By extending the method for constructing multi-solitons of non-integrable nonlinear SchrSdinger equations and systems developed by Martel et al. to the present non-integrable generalized Davey- Stewartson system and overcoming some new difficulties caused by the nonlocal operator B, we prove the existence of multi-solitons for this system. Furthermore, we also give a generalization of this result to a more general class of equations with nonlocal nonlinearities.