In mapping out China’s future development,policymakers must bear in mind the challenges from a falling birthrate and aging society.Based on previous demographic censuses and original data of large sample surveys,this...In mapping out China’s future development,policymakers must bear in mind the challenges from a falling birthrate and aging society.Based on previous demographic censuses and original data of large sample surveys,this study employed an indirect method for estimating China’s total fertility rate(TFR)and a demographic forecast method based on the parity progression ratio(PPR).Our analysis details the socioeconomic implications behind demographic change,and we have proposed public policy countermeasures.The findings include:(i)China’s ultra-low fertility rate over the past three decades has led to an increasingly aging society,and China’s future fertility rate is likely to continue to decrease.(ii)Around 2024,China’s total population is expected to peak at 1.407 billion,followed by chronic negative population growth at an accelerating pace.By 2050,China’s newborn population will shrink to 8.73 million and the aging population will increase to roughly 30%of the total population resulting in the total dependency ratio above 50%.(iii)With a falling birthrate and aging society,China will face unprecedented challenges with respect to education,employment,and pension.(iv)As a public policy response,China should remove birth control altogether.If the fertility rate continues to stall,policymakers should consider issuing policies to encourage birth and create a birth-friendly society for all birth cycles.展开更多
Objective: To study the influences of different time intervals between loop electrosurgical excision (LEEP) and abdominal hysterectomy or radical hysterectomy on postoperative complications. Methods: Sixty-eight p...Objective: To study the influences of different time intervals between loop electrosurgical excision (LEEP) and abdominal hysterectomy or radical hysterectomy on postoperative complications. Methods: Sixty-eight patients, who received subsequent abdominal hysterectomy or radical hysterectomy after LEEP due to C1N III and cervical cancer (IA1, IA2 and IB1), were included in the present study. The hospital and clinic records of these patients were reviewed. The patients were divided into three groups according to the time intervals between LEEP and hysterectomy or radical hysterectomy: group l(within 48 h), group 2 (between 48 h to 6 weeks), and group 3(〉 6 weeks ).Results: General characteristics of patients, including the mean age, delivery history, BMI, menopausal status, clinical stage and HPV infection, were comparable between patients of different groups. There were no significant differences in the mean transfusion amount, posthysterectomy hospital stay or operation time between different groups. The frequencies and spectrum of complications were not significantly affected by the time interval between LEEP and hysterectomy or radical hysterectomy. Conclusion: It is concluded that whenever the LEEP is done, the operation including hysterectomy or radical hysterectomy can be conducted at any time as it is necessary for the patients.展开更多
Children, who are the most weak people about sensibility and telling their desires clearly, act differently at every age phases. Because of this, child oriented designs must appeal to children about whole ages and fea...Children, who are the most weak people about sensibility and telling their desires clearly, act differently at every age phases. Because of this, child oriented designs must appeal to children about whole ages and features. Playgrounds are places where children of all ages and features spend their times commonly. But when these playgrounds were observed, that can be seen, playgrounds can not meet children's need and, because of this, they become quitted places. Within this article, child, play and playground concepts have been examined and the parameters of playground design have been classified. The aim of article is revealing the parameters for playgrounds design and offering a guide so that it can give an idea for the new- designs.展开更多
The insulin/insulin-like growth factor 1 (IGF-1) signaling pathway is evolutionary conserved in diverse speciesincluding C.elegans, saccharomyces cerevisiae, Drosophila melanogaster, rodents and humans, which is invol...The insulin/insulin-like growth factor 1 (IGF-1) signaling pathway is evolutionary conserved in diverse speciesincluding C.elegans, saccharomyces cerevisiae, Drosophila melanogaster, rodents and humans, which is involved in many interrelated functions that are necessary for metabolism, growth and reproduction. Interestingly, more and more research has revealed that insulin/IGF-1 signaling pathway plays a pivotal role in the regulation of longevity. Generally, disruption of the power of this pathway will extend longevity in species ranging from C.elegansto humans. The role of insulin/IGF-1 in longevit yis probably related to stress resistance. Although the underlying mechanisms of longevity are not fully understood, the Insulin/IGF-1 signaling pathway has attracted substantial attention and it will be a novel target to prevent or postpone age-related diseases and extend life span. In this review, we mainly focus on the similar constitution and role of insulin/IGF-1 signaling pathway in C.elegans, saccharomyces cerevisiae, rodents and humans.展开更多
Cadmium-induced DNA degradation in gill cells of the scallop Mizuhopectenyessoensis was assessed using the comet assay (single-cell gel electrophoresis). Accumulation of highly toxic cadmium in the gill cells of biv...Cadmium-induced DNA degradation in gill cells of the scallop Mizuhopectenyessoensis was assessed using the comet assay (single-cell gel electrophoresis). Accumulation of highly toxic cadmium in the gill cells of bivalve is accompanied by the damage of the cell genome revealed as DNA migration in the comet assay. The main mechanisms of Cd effects on the integrity of the DNA structure are discussed.展开更多
Fortifying commonly consumed foods and beverages offers a great opportunity of filling the nutrient gap between actual and the needed nutrient intakes of young children. Ready-to-drink beverage as a vehicle for fortif...Fortifying commonly consumed foods and beverages offers a great opportunity of filling the nutrient gap between actual and the needed nutrient intakes of young children. Ready-to-drink beverage as a vehicle for fortification is easy to administer, more consistent and least obstructive, because it can be consumed without further processing/cooking. This study aimed to evaluate the effectiveness of fortified beverage on the improving of iron status and school performance test of schoolchildren. About 5,000 schoolchildren aged 6-12 years old from 19 schools in Quirino province, Philippines were invited to participate in the study. Samples of 4,875 children with parental consent were screened for hemoglobin and serum ferritin levels. About 4,495 children had complete participation in the school performance test, which were developed and administered by school supervisors. Children were fed 200 mL ready-to-drink juice fortified with iron, zinc, lysine, vitamins A and C for 120 d. The beverage was delivered through the baseline. At end of the intervention, only 1,050 children had complete data set and the anemia rate reduced from 100% to 60%. Iron deficiency has increased insignificantly from 3.6% to 4.5%. Mean percentage of test scores increased significantly between base and end of the study: English (40% to 60%), math (35% to 58%) and science (32% to 58%). In conclusion, consumption of 200 mL fortified juice drink had contributed to the reduction of anemia and increased tests scores of children.展开更多
A bacterial artificial chromosome (BAC) library is an invaluable resource tool to initiate tea plant genomics research, and the preparation of high molecular weight (HMW) genomic DNA is a crucial first step for co...A bacterial artificial chromosome (BAC) library is an invaluable resource tool to initiate tea plant genomics research, and the preparation of high molecular weight (HMW) genomic DNA is a crucial first step for constructing a BAC Library. In order to construct a BAC library for enhancing tea plant genomics research, a new method for the preparation of tea pant high molecular weight (HMW) genomic DNA must be developed due to young tea plant leaves and shoots are notably rich in both tea polyphenols and tea polysaccharides. In this paper, a modified method for preparing high quality tea plant HMW genomi~ DNA was optimized, and the quality of tea plant genomic DNA was evaluated. The results were as follows: Critical indicators of HMW DNA preparation were the appearance of the smooth nuclei in solution (as opposed to sticky-gummy) before agarose plug solidification, non-dark colored nuclei plugs after lysis with an SDS/proteinase K solution, and the quality and quantity of HMW DNA fragments after restriction enzyme digestion. Importantly, 1% dissolved PVP-40 and 1% un-dissolved PVP-40 during the nuclei extraction steps, in conjunction with the removal of PVP-40 from the plug washing and nuclei lysis steps, were critical for achieving HWM tea plant DNA suitable for BAC library construction. Additionally, a third PFGE fraction selection step to eliminate contaminating small DNA fragments. The modifications provided parameters that may have prevented deleterious interactions from tea polyphenols and tea polysaccharides. The HMW genomic DNA produced by this new modified method has been used to successfully construct a large-insert tea plant BAC library, and thus may be suitable for BAC library construction from other plant species that contain similarly interfering compounds.展开更多
The momentum of rapid aging, old-age service supply has become a big problem. Our country also could not escape the threat of this trend, the implementation of the one-child policy, the core of the family, the old lon...The momentum of rapid aging, old-age service supply has become a big problem. Our country also could not escape the threat of this trend, the implementation of the one-child policy, the core of the family, the old lonely man, and the children in the competitive society, under the strong pressure of endowment service supply. Aiming at the problem of community endowment service insufficient supply, scholars have also launched a rich discussion and a lot of research, this article will talk about the definition, the concept of community endowment service supply and supply problems.展开更多
Objective To investigate the clinical features and treatment of placenta previa complicated with previous caesarean section. Methods The clinical data of 29 patients with placenta previa complicated with a previous ca...Objective To investigate the clinical features and treatment of placenta previa complicated with previous caesarean section. Methods The clinical data of 29 patients with placenta previa complicated with a previous caesarean section (RCS group) admitted in Peking Union Medical College Hospital during a period from 2003 to 2011 were retrospectively reviewed and compared with those of 243 patients with placenta previa without a previous caesarean section (FCS group) during the same period. Results There was no difference in the mean age (28.9±3.6 vs. 28.1±4.5 years) and the average gravidity (2.35±1.48 vs. 2.21±1.53) between RCS group and FCS group (all P>0.05). The RCS group had more preterm births (24.1% vs. 13.2%), complete placenta previa (55.2% vs. 4.9%), placenta accreta (34.5% vs. 2.5%), more blood loss during caesarean section (1412±602 vs. 648±265 mL), blood transfusion (51.7% vs. 4.9%), disseminated intravascular coagulation (13.8% vs. 2.1%), and obstetric hysterectomy (13.8% vs. 0.8%) than the FCS group (all P<0.05). The preterm infant rate (30.0% vs. 13.0%), neonatal asphyxia rate (10.0% vs. 4.9%), and perinatal mortality rate (6.7% vs. 0.4%) of the RCS group were higher than those of the FCS group (all P<0.05). Conclusions More patients had complete placenta previa and placenta accreta, postpartum hemorrhage, transfusion, uterine packing, obstetric hysterectomy, and perinatal morbidity in the placenta previa patients with previous caesarean section. The patient should be informed of the risk and unnecessary first cesarean sections should be avoided.展开更多
Micro-filtration membrane (1.2 μm), hyper-filtration membrane (0.45 μm) and nano-filtration membrane (1000 Dalton) were used to characterize the particle size distribution of the organic matters in effluent le...Micro-filtration membrane (1.2 μm), hyper-filtration membrane (0.45 μm) and nano-filtration membrane (1000 Dalton) were used to characterize the particle size distribution of the organic matters in effluent leachate treated by the aged-refuse-bioreactor(ARB) installed at Laogang Refuse Landfill, Shanghai. The results showed that the particle size distribution of the treated fresh and aged leachate using the ARB were quite consistent. The organic matter with particle size below 1000 Dalton accounted for about 90%, balanced with the suspended (〉1.2 μm) and colloidal (1000 Dalton-l.2μm) matters. Hence, it is proposed that coagulation, nano-filtration and electrically chemical oxidation may be not applicable for the advanced treatment for the effluents to reach the discharge standard limit value defined in Chinese national Standard for Pollution Control on the Landfill Site of Municipal Solid Wastes (GB16889-2008), CODCr〈100 mg/L. Aged refuse bioreactor combined reverse osmosis was suggested as an economically and technically feasible technology to treat sanitary landfill leachate.展开更多
AIM: To determine the efficacy of our therapeutic strategy for Helicobacter pylori (H. pylori) eradication and to identify predictive factors for successful eradication. METHODS: From April 2006 to June 2010, we retro...AIM: To determine the efficacy of our therapeutic strategy for Helicobacter pylori (H. pylori) eradication and to identify predictive factors for successful eradication. METHODS: From April 2006 to June 2010, we retrospectively assessed 2428 consecutive patients (1025 men, 1403 women; mean age 55 years, age range 18-92 years) with gastric histology positive for H. pylori infection referred to our unit for 13-C urea breath test(UBT), after first-line therapy with proton pump inhibitor (PPI) b.i.d. + amoxicillin 1 g b.i.d. + clarithromycin 500 mg b.i.d. for 7 d. Patients who were still positive to UBT were recommended a second-line therapy (PPI b.i.d. + amoxicillin 1 g b.i.d. + tinidazole 500 mg b.i.d. for 14 d). Third choice treatment was empirical with PPI b.i.d. + amoxicillin 1 g b.i.d. + levofloxacin 250 mg b.i.d. for 14 d. RESULTS: Out of 614 patients, still H. pylori-positive after first-line therapy, only 326 and 19 patients respectively rechecked their H. pylori status by UBT after the suggested second and third-line regimens. "Per protocol" eradication rates for first, second and thirdline therapy were 74.7% (95% CI: 72.7%-76.4%), 85.3% (95% CI: 81.1%-89.1%) and 89.5% (95% CI: 74.9%-103%) respectively. The overall percentage of patients with H. pylori eradicated after two treatments was 97.8% (95% CI: 97.1%-98.4%), vs 99.9% (95% CI: 99.8%-100%) after three treatments. The study found that eradication therapy was most effective in patients with ulcer disease (P < 0.05, P = 0.028), especially in those with duodenal ulcer. Smoking habits did not significantly affect the eradication rate. CONCLUSION: First-line therapy with amoxicillin and clarithromycin produces an H. pylori eradication rate comparable or superior to other studies and secondline treatment can still be triple therapy with amoxicillin and tinidazole.展开更多
The fatigue behaviors of 2E12 aluminum alloy in T3 and T6 conditions at room temperature in air were investigated.The microstructures and fatigue fracture surfaces of the alloy were examined by transmission electron m...The fatigue behaviors of 2E12 aluminum alloy in T3 and T6 conditions at room temperature in air were investigated.The microstructures and fatigue fracture surfaces of the alloy were examined by transmission electron microscopy(TEM) and scanning electron microscopy(SEM).The results show that the alloy exhibits higher fatigue crack propagation(FCP) resistance in T3 condition than in T6 condition,the fatigue life is increased by 54% and the fatigue crack growth rate(FCGR) decreases significantly.The fatigue fractures of the alloy in T3 and T6 conditions are transgranular.But in T3 condition,secondary cracks occur and fatigue striations are not clear.In T6 condition,ductile fatigue striations are observed.The effect of aging conditions on fatigue behaviors is explained in terms of the slip planarity of dislocations and the cyclic slip reversibility.展开更多
AIM:To investigate endoscopic findings in patients with Schatzki rings(SRs) with a focus on evidence for eosinophilic esophagitis(EoE).METHODS:We consecutively approached all adult patients scheduled for elective outp...AIM:To investigate endoscopic findings in patients with Schatzki rings(SRs) with a focus on evidence for eosinophilic esophagitis(EoE).METHODS:We consecutively approached all adult patients scheduled for elective outpatient upper endoscopy for a variety of indications at the German Diagnostic Clinic,Wiesbaden,Germany between July 2007 and July 2010.All patients with endoscopically diagnosed SRs,defined as thin,symmetrical,mucosal structures located at the esophagogastric junction,were prospectively registered.Additional endoscopic findings,clinical information and histopathological findings with a focus on esophageal eosinophilia(≥ 20 eosinophils/high power field) were recorded.The criteria for active EoE were defined as:(1) eosinophilic tissue infiltration ≥ 20 eosinophils/hpf;(2) symptoms of esophageal dysfunction;and(3) exclusion of other causes of esophageal eosinophilia.Gastroesophageal reflux disease was excluded by proton pump inhibitor treatment prior to endoscopy.The presence of ≥ 20 eosinophils/hpf in esophageal biopsies in patients that did not fulfil the criteria of EoE was defined as esophageal hypereosinophilia.RESULTS:A SR was diagnosed in 171(3.3%;128 males,43 females,mean age 66 ± 12.9 years) of the 5163 patients that underwent upper gastrointestinalendoscopy.Twenty of the 116 patients(17%) from whom esophageal biopsies were obtained showed histological hypereosinophilia(≥ 20 eosinophils/hpf).Nine of these patients(8 males,1 female,mean age 49 ± 10 years) did not fulfill all diagnostic criteria of EoE,whereas in 11(9%) patients with ≥ 20 eosinophils/hpf,a definite diagnosis of EoE was made.Three of the 11 patients(27%) with definite EoE had no suspicious endoscopic features of EoE.In contrast,in the 25 patients in whom EoE was suspected by endoscopic features,EoE was only confirmed in 7(28%) patients.Patients with EoE were younger(mean age 41.5 ± 6.5 vs 50.5 ± 11.5 years,P = 0.012),were more likely to have a history of allergies(73% vs 29%,P = 0.007) and complained more often of dysphagia(91% vs 34%,P = 0.004) and food impaction(36% vs 6%,P = 0.007) than patients without EoE.Endoscopically,additional webs were found significantly more often in patients with EoE than in patients without EoE(36% vs 11%,P = 0.04).Furthermore,the SR had a tendency to be narrower in patients with EoE than in those without EoE(36% vs 18%,P = 0.22).The percentage of males(73% vs 72%,P = 1.0) and frequency of heartburn(27% vs 27%,P = 1.0) were not significantly different in both groups.The 9 patients with esophageal hypereosinophilia that did not fulfil the diagnostic criteria of EoE were younger(mean age 49 ± 10 years vs 58 ± 6 years,P = 0.0008) and were more likely to have a history of allergies(78% vs 24%,P = 0.003) than patients with < 20 eosinophils/hpf.Predictors of EoE were younger age,presence of dysphagia or food impaction and a history of allergies.CONCLUSION:A significant proportion of patients with SRs also have EoE,which may not always be suspected according to other endoscopic features.展开更多
Objective To analyze the clinical effects after one year follow-up of LASIK for myopia. Methods According to the preoperative diopters of the myopia, patients, undergoing LASIK, were divided into three groups, group ...Objective To analyze the clinical effects after one year follow-up of LASIK for myopia. Methods According to the preoperative diopters of the myopia, patients, undergoing LASIK, were divided into three groups, group Ⅰ: 140 eyes ( -1. 25D ~ -6. 00 D) , group Ⅱ : 205 eyes ( -6. 25D ~ -10. 00D) , group Ⅲ: 226 eyes ( -10.25D- -15.00D). The patients were followed up at least I year. Results By groups, the percentage of the uncorrected visual acuity equal or more than 1. 0 were 94. 29% , 80. 30% , 42. 04% respectively. The residual refractive error within 0. 5D were 99. 29% , 85. 71% , 59. 70%. The spherical equivalent was stable 3 months after LASIK. The complications were few. Conclusion LASIK is a safe, effective and good predictable method for the treatment of myopia. It is one of the best choices to treat myopia below -10. 00D.展开更多
The museum education focusing on the children is different from adult acceptors for the mental development of the children presents obvious stage features. Therefore, a clear definition on the ages of the children in ...The museum education focusing on the children is different from adult acceptors for the mental development of the children presents obvious stage features. Therefore, a clear definition on the ages of the children in the service must be made. The children in different ages can find their own educational project groups in a short time according to their own categories and ages. To facilitate the branding of routine childhood education programs, improve the implementing benefit of childhood education programs and realize the maximization of resource utilization in the Museum Children Education, this study firstly generalizes and extracts one of the common problems that affect the implementing benefit of Chinese Museum Children Education project based on the case analysis - focus and age-group of service objects. Then this paper (article) explores it step by step. Finally the article combines the analyses with the empirical researches on feedback information acquired in on-site observations and semi-structured interviews, to propose improvement suggestions for the problem.展开更多
Through the scientific investigation on Lushan for a long time, detailed studies have been carried out on the geologic stratum section of Late Quaternary in Dajiaochang. The series age data of complete stratigraphic s...Through the scientific investigation on Lushan for a long time, detailed studies have been carried out on the geologic stratum section of Late Quaternary in Dajiaochang. The series age data of complete stratigraphic section and full time formation since 400 kaBP were based on the results of the analyses using the dating methods with various instruments such as the ancient geomagnetism, electronics spin resonance (ESR), 36C1 and lumines- cence dating, etc. Corresponding to these data, it identified the paleomagnetic polarity events including Biwa- III event (320 kaBP), Biwa- 1I event (260 kaBP), Biwa- I event (180 kaBP, scarcity due to the disturbance of the iron dish), Blake event (100 kaBP) and Laschamp event (20 kaBP, didn't grow due to the activities of the surface), etc. Combined with the sequence stratigraphy, layer type characteristics and its sediment environment, the strati- graphic can be divided into 4 stages of development respectively : Stage [ is the climate period of glacial epoch (200-400 kaBP); stage 11 is the climate period of interglacial epoch (100-200 kaBP); stage llI is the climate peri- od of periglacial epoch (10-100 kaBP); stage IV is the climate period ofpostglacial epoch (0-10 kaBP).展开更多
To investigate the status of mineral metabolism in people residing in huge regional centers of north-western and north-eastern parts of Russia by AES-IBP (atom-emission spectrometry with inductively bonded argon pla...To investigate the status of mineral metabolism in people residing in huge regional centers of north-western and north-eastern parts of Russia by AES-IBP (atom-emission spectrometry with inductively bonded argon plasma), the content of 25 elements in hair samples of surveyed people was examined. The results of the study carried out in Russia's north could testify to significant deformation found in the elderly people bodies' element profiles. The pronounced deficit in such essential elements as Ca, Cr, Mg can be considered the common north-related profile typical for the elderly people from European and Asian north. In addition, age-related formation of all the elements' deficit due to their risen spending at pathologies as well as the increased need in such elements as Zn, Se, Cr, Cu, Mn. The age-related excess of arsenic proved to be typical for Magadan residents. In Anadyr, the age-related excess of lead was found, in Petrozavodsk mercury, and in Arkhangelsk--boron and aluminium.展开更多
文摘In mapping out China’s future development,policymakers must bear in mind the challenges from a falling birthrate and aging society.Based on previous demographic censuses and original data of large sample surveys,this study employed an indirect method for estimating China’s total fertility rate(TFR)and a demographic forecast method based on the parity progression ratio(PPR).Our analysis details the socioeconomic implications behind demographic change,and we have proposed public policy countermeasures.The findings include:(i)China’s ultra-low fertility rate over the past three decades has led to an increasingly aging society,and China’s future fertility rate is likely to continue to decrease.(ii)Around 2024,China’s total population is expected to peak at 1.407 billion,followed by chronic negative population growth at an accelerating pace.By 2050,China’s newborn population will shrink to 8.73 million and the aging population will increase to roughly 30%of the total population resulting in the total dependency ratio above 50%.(iii)With a falling birthrate and aging society,China will face unprecedented challenges with respect to education,employment,and pension.(iv)As a public policy response,China should remove birth control altogether.If the fertility rate continues to stall,policymakers should consider issuing policies to encourage birth and create a birth-friendly society for all birth cycles.
文摘Objective: To study the influences of different time intervals between loop electrosurgical excision (LEEP) and abdominal hysterectomy or radical hysterectomy on postoperative complications. Methods: Sixty-eight patients, who received subsequent abdominal hysterectomy or radical hysterectomy after LEEP due to C1N III and cervical cancer (IA1, IA2 and IB1), were included in the present study. The hospital and clinic records of these patients were reviewed. The patients were divided into three groups according to the time intervals between LEEP and hysterectomy or radical hysterectomy: group l(within 48 h), group 2 (between 48 h to 6 weeks), and group 3(〉 6 weeks ).Results: General characteristics of patients, including the mean age, delivery history, BMI, menopausal status, clinical stage and HPV infection, were comparable between patients of different groups. There were no significant differences in the mean transfusion amount, posthysterectomy hospital stay or operation time between different groups. The frequencies and spectrum of complications were not significantly affected by the time interval between LEEP and hysterectomy or radical hysterectomy. Conclusion: It is concluded that whenever the LEEP is done, the operation including hysterectomy or radical hysterectomy can be conducted at any time as it is necessary for the patients.
文摘Children, who are the most weak people about sensibility and telling their desires clearly, act differently at every age phases. Because of this, child oriented designs must appeal to children about whole ages and features. Playgrounds are places where children of all ages and features spend their times commonly. But when these playgrounds were observed, that can be seen, playgrounds can not meet children's need and, because of this, they become quitted places. Within this article, child, play and playground concepts have been examined and the parameters of playground design have been classified. The aim of article is revealing the parameters for playgrounds design and offering a guide so that it can give an idea for the new- designs.
基金Supported by the Major State Basic Research Development Program of China (973 Program), No. G2000057010
文摘The insulin/insulin-like growth factor 1 (IGF-1) signaling pathway is evolutionary conserved in diverse speciesincluding C.elegans, saccharomyces cerevisiae, Drosophila melanogaster, rodents and humans, which is involved in many interrelated functions that are necessary for metabolism, growth and reproduction. Interestingly, more and more research has revealed that insulin/IGF-1 signaling pathway plays a pivotal role in the regulation of longevity. Generally, disruption of the power of this pathway will extend longevity in species ranging from C.elegansto humans. The role of insulin/IGF-1 in longevit yis probably related to stress resistance. Although the underlying mechanisms of longevity are not fully understood, the Insulin/IGF-1 signaling pathway has attracted substantial attention and it will be a novel target to prevent or postpone age-related diseases and extend life span. In this review, we mainly focus on the similar constitution and role of insulin/IGF-1 signaling pathway in C.elegans, saccharomyces cerevisiae, rodents and humans.
文摘Cadmium-induced DNA degradation in gill cells of the scallop Mizuhopectenyessoensis was assessed using the comet assay (single-cell gel electrophoresis). Accumulation of highly toxic cadmium in the gill cells of bivalve is accompanied by the damage of the cell genome revealed as DNA migration in the comet assay. The main mechanisms of Cd effects on the integrity of the DNA structure are discussed.
文摘Fortifying commonly consumed foods and beverages offers a great opportunity of filling the nutrient gap between actual and the needed nutrient intakes of young children. Ready-to-drink beverage as a vehicle for fortification is easy to administer, more consistent and least obstructive, because it can be consumed without further processing/cooking. This study aimed to evaluate the effectiveness of fortified beverage on the improving of iron status and school performance test of schoolchildren. About 5,000 schoolchildren aged 6-12 years old from 19 schools in Quirino province, Philippines were invited to participate in the study. Samples of 4,875 children with parental consent were screened for hemoglobin and serum ferritin levels. About 4,495 children had complete participation in the school performance test, which were developed and administered by school supervisors. Children were fed 200 mL ready-to-drink juice fortified with iron, zinc, lysine, vitamins A and C for 120 d. The beverage was delivered through the baseline. At end of the intervention, only 1,050 children had complete data set and the anemia rate reduced from 100% to 60%. Iron deficiency has increased insignificantly from 3.6% to 4.5%. Mean percentage of test scores increased significantly between base and end of the study: English (40% to 60%), math (35% to 58%) and science (32% to 58%). In conclusion, consumption of 200 mL fortified juice drink had contributed to the reduction of anemia and increased tests scores of children.
文摘A bacterial artificial chromosome (BAC) library is an invaluable resource tool to initiate tea plant genomics research, and the preparation of high molecular weight (HMW) genomic DNA is a crucial first step for constructing a BAC Library. In order to construct a BAC library for enhancing tea plant genomics research, a new method for the preparation of tea pant high molecular weight (HMW) genomic DNA must be developed due to young tea plant leaves and shoots are notably rich in both tea polyphenols and tea polysaccharides. In this paper, a modified method for preparing high quality tea plant HMW genomi~ DNA was optimized, and the quality of tea plant genomic DNA was evaluated. The results were as follows: Critical indicators of HMW DNA preparation were the appearance of the smooth nuclei in solution (as opposed to sticky-gummy) before agarose plug solidification, non-dark colored nuclei plugs after lysis with an SDS/proteinase K solution, and the quality and quantity of HMW DNA fragments after restriction enzyme digestion. Importantly, 1% dissolved PVP-40 and 1% un-dissolved PVP-40 during the nuclei extraction steps, in conjunction with the removal of PVP-40 from the plug washing and nuclei lysis steps, were critical for achieving HWM tea plant DNA suitable for BAC library construction. Additionally, a third PFGE fraction selection step to eliminate contaminating small DNA fragments. The modifications provided parameters that may have prevented deleterious interactions from tea polyphenols and tea polysaccharides. The HMW genomic DNA produced by this new modified method has been used to successfully construct a large-insert tea plant BAC library, and thus may be suitable for BAC library construction from other plant species that contain similarly interfering compounds.
文摘The momentum of rapid aging, old-age service supply has become a big problem. Our country also could not escape the threat of this trend, the implementation of the one-child policy, the core of the family, the old lonely man, and the children in the competitive society, under the strong pressure of endowment service supply. Aiming at the problem of community endowment service insufficient supply, scholars have also launched a rich discussion and a lot of research, this article will talk about the definition, the concept of community endowment service supply and supply problems.
基金Supported by Beijing Clinical Study(Z111107058811025)Beijing Government Excellent Person Sponsor Program
文摘Objective To investigate the clinical features and treatment of placenta previa complicated with previous caesarean section. Methods The clinical data of 29 patients with placenta previa complicated with a previous caesarean section (RCS group) admitted in Peking Union Medical College Hospital during a period from 2003 to 2011 were retrospectively reviewed and compared with those of 243 patients with placenta previa without a previous caesarean section (FCS group) during the same period. Results There was no difference in the mean age (28.9±3.6 vs. 28.1±4.5 years) and the average gravidity (2.35±1.48 vs. 2.21±1.53) between RCS group and FCS group (all P>0.05). The RCS group had more preterm births (24.1% vs. 13.2%), complete placenta previa (55.2% vs. 4.9%), placenta accreta (34.5% vs. 2.5%), more blood loss during caesarean section (1412±602 vs. 648±265 mL), blood transfusion (51.7% vs. 4.9%), disseminated intravascular coagulation (13.8% vs. 2.1%), and obstetric hysterectomy (13.8% vs. 0.8%) than the FCS group (all P<0.05). The preterm infant rate (30.0% vs. 13.0%), neonatal asphyxia rate (10.0% vs. 4.9%), and perinatal mortality rate (6.7% vs. 0.4%) of the RCS group were higher than those of the FCS group (all P<0.05). Conclusions More patients had complete placenta previa and placenta accreta, postpartum hemorrhage, transfusion, uterine packing, obstetric hysterectomy, and perinatal morbidity in the placenta previa patients with previous caesarean section. The patient should be informed of the risk and unnecessary first cesarean sections should be avoided.
基金Acknowledgments: This work was financially supported by Science and Technology Support Plan of The Ministry of Science and Technology of People's Republic of China (No. 2006BAC02A19) China National Natural Science Foundation with Grant (No. 20177014).
文摘Micro-filtration membrane (1.2 μm), hyper-filtration membrane (0.45 μm) and nano-filtration membrane (1000 Dalton) were used to characterize the particle size distribution of the organic matters in effluent leachate treated by the aged-refuse-bioreactor(ARB) installed at Laogang Refuse Landfill, Shanghai. The results showed that the particle size distribution of the treated fresh and aged leachate using the ARB were quite consistent. The organic matter with particle size below 1000 Dalton accounted for about 90%, balanced with the suspended (〉1.2 μm) and colloidal (1000 Dalton-l.2μm) matters. Hence, it is proposed that coagulation, nano-filtration and electrically chemical oxidation may be not applicable for the advanced treatment for the effluents to reach the discharge standard limit value defined in Chinese national Standard for Pollution Control on the Landfill Site of Municipal Solid Wastes (GB16889-2008), CODCr〈100 mg/L. Aged refuse bioreactor combined reverse osmosis was suggested as an economically and technically feasible technology to treat sanitary landfill leachate.
文摘AIM: To determine the efficacy of our therapeutic strategy for Helicobacter pylori (H. pylori) eradication and to identify predictive factors for successful eradication. METHODS: From April 2006 to June 2010, we retrospectively assessed 2428 consecutive patients (1025 men, 1403 women; mean age 55 years, age range 18-92 years) with gastric histology positive for H. pylori infection referred to our unit for 13-C urea breath test(UBT), after first-line therapy with proton pump inhibitor (PPI) b.i.d. + amoxicillin 1 g b.i.d. + clarithromycin 500 mg b.i.d. for 7 d. Patients who were still positive to UBT were recommended a second-line therapy (PPI b.i.d. + amoxicillin 1 g b.i.d. + tinidazole 500 mg b.i.d. for 14 d). Third choice treatment was empirical with PPI b.i.d. + amoxicillin 1 g b.i.d. + levofloxacin 250 mg b.i.d. for 14 d. RESULTS: Out of 614 patients, still H. pylori-positive after first-line therapy, only 326 and 19 patients respectively rechecked their H. pylori status by UBT after the suggested second and third-line regimens. "Per protocol" eradication rates for first, second and thirdline therapy were 74.7% (95% CI: 72.7%-76.4%), 85.3% (95% CI: 81.1%-89.1%) and 89.5% (95% CI: 74.9%-103%) respectively. The overall percentage of patients with H. pylori eradicated after two treatments was 97.8% (95% CI: 97.1%-98.4%), vs 99.9% (95% CI: 99.8%-100%) after three treatments. The study found that eradication therapy was most effective in patients with ulcer disease (P < 0.05, P = 0.028), especially in those with duodenal ulcer. Smoking habits did not significantly affect the eradication rate. CONCLUSION: First-line therapy with amoxicillin and clarithromycin produces an H. pylori eradication rate comparable or superior to other studies and secondline treatment can still be triple therapy with amoxicillin and tinidazole.
基金Project(2005CB623705) supported by the National Basic Research Program of China
文摘The fatigue behaviors of 2E12 aluminum alloy in T3 and T6 conditions at room temperature in air were investigated.The microstructures and fatigue fracture surfaces of the alloy were examined by transmission electron microscopy(TEM) and scanning electron microscopy(SEM).The results show that the alloy exhibits higher fatigue crack propagation(FCP) resistance in T3 condition than in T6 condition,the fatigue life is increased by 54% and the fatigue crack growth rate(FCGR) decreases significantly.The fatigue fractures of the alloy in T3 and T6 conditions are transgranular.But in T3 condition,secondary cracks occur and fatigue striations are not clear.In T6 condition,ductile fatigue striations are observed.The effect of aging conditions on fatigue behaviors is explained in terms of the slip planarity of dislocations and the cyclic slip reversibility.
文摘AIM:To investigate endoscopic findings in patients with Schatzki rings(SRs) with a focus on evidence for eosinophilic esophagitis(EoE).METHODS:We consecutively approached all adult patients scheduled for elective outpatient upper endoscopy for a variety of indications at the German Diagnostic Clinic,Wiesbaden,Germany between July 2007 and July 2010.All patients with endoscopically diagnosed SRs,defined as thin,symmetrical,mucosal structures located at the esophagogastric junction,were prospectively registered.Additional endoscopic findings,clinical information and histopathological findings with a focus on esophageal eosinophilia(≥ 20 eosinophils/high power field) were recorded.The criteria for active EoE were defined as:(1) eosinophilic tissue infiltration ≥ 20 eosinophils/hpf;(2) symptoms of esophageal dysfunction;and(3) exclusion of other causes of esophageal eosinophilia.Gastroesophageal reflux disease was excluded by proton pump inhibitor treatment prior to endoscopy.The presence of ≥ 20 eosinophils/hpf in esophageal biopsies in patients that did not fulfil the criteria of EoE was defined as esophageal hypereosinophilia.RESULTS:A SR was diagnosed in 171(3.3%;128 males,43 females,mean age 66 ± 12.9 years) of the 5163 patients that underwent upper gastrointestinalendoscopy.Twenty of the 116 patients(17%) from whom esophageal biopsies were obtained showed histological hypereosinophilia(≥ 20 eosinophils/hpf).Nine of these patients(8 males,1 female,mean age 49 ± 10 years) did not fulfill all diagnostic criteria of EoE,whereas in 11(9%) patients with ≥ 20 eosinophils/hpf,a definite diagnosis of EoE was made.Three of the 11 patients(27%) with definite EoE had no suspicious endoscopic features of EoE.In contrast,in the 25 patients in whom EoE was suspected by endoscopic features,EoE was only confirmed in 7(28%) patients.Patients with EoE were younger(mean age 41.5 ± 6.5 vs 50.5 ± 11.5 years,P = 0.012),were more likely to have a history of allergies(73% vs 29%,P = 0.007) and complained more often of dysphagia(91% vs 34%,P = 0.004) and food impaction(36% vs 6%,P = 0.007) than patients without EoE.Endoscopically,additional webs were found significantly more often in patients with EoE than in patients without EoE(36% vs 11%,P = 0.04).Furthermore,the SR had a tendency to be narrower in patients with EoE than in those without EoE(36% vs 18%,P = 0.22).The percentage of males(73% vs 72%,P = 1.0) and frequency of heartburn(27% vs 27%,P = 1.0) were not significantly different in both groups.The 9 patients with esophageal hypereosinophilia that did not fulfil the diagnostic criteria of EoE were younger(mean age 49 ± 10 years vs 58 ± 6 years,P = 0.0008) and were more likely to have a history of allergies(78% vs 24%,P = 0.003) than patients with < 20 eosinophils/hpf.Predictors of EoE were younger age,presence of dysphagia or food impaction and a history of allergies.CONCLUSION:A significant proportion of patients with SRs also have EoE,which may not always be suspected according to other endoscopic features.
文摘Objective To analyze the clinical effects after one year follow-up of LASIK for myopia. Methods According to the preoperative diopters of the myopia, patients, undergoing LASIK, were divided into three groups, group Ⅰ: 140 eyes ( -1. 25D ~ -6. 00 D) , group Ⅱ : 205 eyes ( -6. 25D ~ -10. 00D) , group Ⅲ: 226 eyes ( -10.25D- -15.00D). The patients were followed up at least I year. Results By groups, the percentage of the uncorrected visual acuity equal or more than 1. 0 were 94. 29% , 80. 30% , 42. 04% respectively. The residual refractive error within 0. 5D were 99. 29% , 85. 71% , 59. 70%. The spherical equivalent was stable 3 months after LASIK. The complications were few. Conclusion LASIK is a safe, effective and good predictable method for the treatment of myopia. It is one of the best choices to treat myopia below -10. 00D.
文摘The museum education focusing on the children is different from adult acceptors for the mental development of the children presents obvious stage features. Therefore, a clear definition on the ages of the children in the service must be made. The children in different ages can find their own educational project groups in a short time according to their own categories and ages. To facilitate the branding of routine childhood education programs, improve the implementing benefit of childhood education programs and realize the maximization of resource utilization in the Museum Children Education, this study firstly generalizes and extracts one of the common problems that affect the implementing benefit of Chinese Museum Children Education project based on the case analysis - focus and age-group of service objects. Then this paper (article) explores it step by step. Finally the article combines the analyses with the empirical researches on feedback information acquired in on-site observations and semi-structured interviews, to propose improvement suggestions for the problem.
基金Open Foundation for State Key Laboratory of Loess and Quaternary Geology,Institute of Earth Environment,Chinese Academy of Sciences(No.SKLLQG0701)Hunan Province Key Subject Construction Item(No.ZRDL0701)Open Foundation for State Key Laboratory of Continental Dynamics,Northwest University(No.DL2006001)
文摘Through the scientific investigation on Lushan for a long time, detailed studies have been carried out on the geologic stratum section of Late Quaternary in Dajiaochang. The series age data of complete stratigraphic section and full time formation since 400 kaBP were based on the results of the analyses using the dating methods with various instruments such as the ancient geomagnetism, electronics spin resonance (ESR), 36C1 and lumines- cence dating, etc. Corresponding to these data, it identified the paleomagnetic polarity events including Biwa- III event (320 kaBP), Biwa- 1I event (260 kaBP), Biwa- I event (180 kaBP, scarcity due to the disturbance of the iron dish), Blake event (100 kaBP) and Laschamp event (20 kaBP, didn't grow due to the activities of the surface), etc. Combined with the sequence stratigraphy, layer type characteristics and its sediment environment, the strati- graphic can be divided into 4 stages of development respectively : Stage [ is the climate period of glacial epoch (200-400 kaBP); stage 11 is the climate period of interglacial epoch (100-200 kaBP); stage llI is the climate peri- od of periglacial epoch (10-100 kaBP); stage IV is the climate period ofpostglacial epoch (0-10 kaBP).
文摘To investigate the status of mineral metabolism in people residing in huge regional centers of north-western and north-eastern parts of Russia by AES-IBP (atom-emission spectrometry with inductively bonded argon plasma), the content of 25 elements in hair samples of surveyed people was examined. The results of the study carried out in Russia's north could testify to significant deformation found in the elderly people bodies' element profiles. The pronounced deficit in such essential elements as Ca, Cr, Mg can be considered the common north-related profile typical for the elderly people from European and Asian north. In addition, age-related formation of all the elements' deficit due to their risen spending at pathologies as well as the increased need in such elements as Zn, Se, Cr, Cu, Mn. The age-related excess of arsenic proved to be typical for Magadan residents. In Anadyr, the age-related excess of lead was found, in Petrozavodsk mercury, and in Arkhangelsk--boron and aluminium.