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哺乳动物卵母细胞孤雌激活及孤雌胚发育研究进展 被引量:4
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作者 张果平 黄永宏 李峰 《内蒙古畜牧科学》 2003年第1期32-34,共3页
简述了哺乳动物卵母细胞孤雌激活的方法和影响因素、孤雌激活卵的核型、孤雌激活的机制及孤雌胚体内外发育研究的进展情况,并指出了雌性和雄性基因组在个体发育中的作用以及孤雌生殖的意义。
关键词 哺乳动物 卵母细胞 孤雌激活 孤雌胚发育
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不同冷冻方法对牛卵母细胞及孤雌胚发育的影响
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作者 卞江涛 倪明 +6 位作者 胡高博 赵鑫 吕自力 卢全晟 薛利 宋彦军 张居农 《中国草食动物》 2010年第1期12-15,共4页
研究了麦管和OPS管法冷冻以及OPS法中保护剂种类对牛卵母细胞体外成熟及孤雌胚发育的影响。结果发现,OPS管冷冻牛卵母细胞形态正常率、卵裂率、囊胚率极显著高于麦管法(P<0.05)。在OPS法中,应用两种保护剂冷冻后,卵母细胞形态正常率... 研究了麦管和OPS管法冷冻以及OPS法中保护剂种类对牛卵母细胞体外成熟及孤雌胚发育的影响。结果发现,OPS管冷冻牛卵母细胞形态正常率、卵裂率、囊胚率极显著高于麦管法(P<0.05)。在OPS法中,应用两种保护剂冷冻后,卵母细胞形态正常率、卵裂率、囊胚率差异极显著(P<0.01);采取38℃与25℃温度平衡,冷冻后卵母细胞各发育指标差异不显著(P>0.05);采用4℃平衡,冷冻后的卵母细胞激活后没有出现囊胚,各发育指标极显著降低(P<0.01)。结果表明,OPS法可以有效地保护卵母细胞,保证其后孤雌激活;采用低温平衡会对孤雌发育的囊胚阶段有较大影响。 展开更多
关键词 冷冻方法 牛卵母细胞 体外成熟 孤雌胚发育
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Nuclear reprogramming: the strategy used in normal development is also used in somatic cell nuclear transfer and parthenogenesis 被引量:1
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作者 Tianlong Gao Junke Zheng +7 位作者 Fengying Xing Haiyan Fang Feng Sun Ayong Yan Xun Gong Hui Ding Fan Tang Hui Z Sheng 《Cell Research》 SCIE CAS CSCD 2007年第2期135-150,共16页
Somatic cell nuclear transfer (SCNT) and parthenogenesis are alternative forms of reproduction and development, building new life cycles on differentiated somatic cell nuclei and duplicated maternal chromatin, respe... Somatic cell nuclear transfer (SCNT) and parthenogenesis are alternative forms of reproduction and development, building new life cycles on differentiated somatic cell nuclei and duplicated maternal chromatin, respectively. In the preceding paper (Sun F, et al., Cell Res 2007; 17:117-134.), we showed that an "erase-and-rebuild" strategy is used in normal development to transform the maternal gene expression profile to a zygotic one. Here, we investigate if the same strategy also applies to SCNT and parthenogenesis. The relationship between chromatin and chromatin factors (CFs) during SCNT and parthenogenesis was examined using immunochemical and GFP-fusion protein assays. Results from these studies indicated that soon after nuclear transfer, a majority of CFs dissociated from somatic nuclei and were redistributed to the cytoplasm of the egg. The erasure process in oogenesis is recaptured during the initial phase in SCNT. Most CFs entered pseudo-pronuclei shortly after their formation. In parthenogenesis, all parthenogenotes underwent normal oogenesis, and thus had removed most CFs from chromosomes before the initiation of development. The CFs were subsequently re-associated with female pronuclei in time and sequence similar to that in fertilized embryos. Based on these data, we conclude that the "erase-and-rebuild" process observed in normal development also occurs in SCNT and in parthenogenesis, albeit in altered fashions. The process is responsible for transcription reprogramming in these procedures. The "erase" process in SCNT is compressed and the efficiency is compromised, which likely contribute to the developmental defects often observed in nuclear transfer (nt) embryos. Furthermore, results from this study indicated that the cytoplasm of an egg contains most, if not all, essential components for assembling the zygotic program and can assemble them onto appropriate diploid chromatin of distinct origins. 展开更多
关键词 nuclear reprogramming SCNT PARTHENOGENESIS chromatin factors CHROMATIN EMBRYOGENESIS
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Shape change in viable eggs of the collembolan Folsomia candida provides insight into the role of Wolbachia endosymbionts
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作者 Nina Hafer Nathan Pike 《Zoological Research》 CAS CSCD 北大核心 2010年第6期623-626,共4页
The endosymbiotic bacteria of the genus Wolbachia that infect the collembolan species Folsomia candida are responsible for facilitating parthenogenetic reproduction in their hosts. This study made empirical observatio... The endosymbiotic bacteria of the genus Wolbachia that infect the collembolan species Folsomia candida are responsible for facilitating parthenogenetic reproduction in their hosts. This study made empirical observations of the development of eggs of F. candida which contained normal populations of Wolbachia and of eggs which were cured of Wolbachia by treatment with the antibiotic rifampicin. A marked increase in egg size accompanied by a significant change in shape from spherical to discoid occurred in viable eggs three to four days after laying. These changes did not occur in the universally inviable eggs which came from the antibiotic treatment or in the 7% of untreated eggs which were naturally inviable. We infer that Wolbachia plays a critical role in zygotic or embryonic development during or before the first three days after laying and we draw on existing knowledge in speculating on the developmental mechanisms that Wolbachia may influence. 展开更多
关键词 PARTHENOGENESIS embryonic development COLLEMBOLA hatching rate
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