在初中英语教学中,笔者通过利用现代信息技术（Modern information technology）与＂导学式（Guided teaching method）＂相结合,对英语教学进行了改革尝试。实践证明,现代信息技术与＂导学式＂融合,像是给予了学生学习英语的＂点金术...在初中英语教学中,笔者通过利用现代信息技术（Modern information technology）与＂导学式（Guided teaching method）＂相结合,对英语教学进行了改革尝试。实践证明,现代信息技术与＂导学式＂融合,像是给予了学生学习英语的＂点金术＂——使课堂教学改变了以往注入式教学的各种不良现象,使学生完全成为学习的主人,展开更多
With the continuous progress on affinity peptide research, it has become more and more popular in pharmacology and medicine. lt is promising to study these viruses affinity peptide to treat infectious diseases. And th...With the continuous progress on affinity peptide research, it has become more and more popular in pharmacology and medicine. lt is promising to study these viruses affinity peptide to treat infectious diseases. And the analysis on the virus affinity peptide with high selectivity and high sensitivity could provide valuable means for disease detection, treatment as wel as the study on the molecular mechanism of virus affinity peptide. Therefore, we reviewed the bioinformatics pre-diction technologies of computer simulation, molecular docking and homology model-ing, as wel as the research method on analyzing and screening virus affinity pep-tide, such as Phage display technology.展开更多
Modern science and technology is being developed towards destroying human and human calls for a new era of science ＆ technology. Contemporary science is seeing a significant breakthrough and a new revolution of scien...Modern science and technology is being developed towards destroying human and human calls for a new era of science ＆ technology. Contemporary science is seeing a significant breakthrough and a new revolution of science and technology is beginning. The theoretical basis of the new revolution of science and technology is ＂state space-time＂, ＂the unity of physics and biology＂, ＂thinking motion and automatic organization of the universe＂ and ＂thinking＇s active control＂. The new revolution of science and technology takes material （microscopic particle） science, life （organic and inorganic life） science and thinking science as the fundamental sciences, takes life technology, new information technology, new material technology and new energy technology as the basis of the overall technological system, progresses in all aspects, including life technology, medicine, new material technology, new information technology, new energy technology, new robot technology, new manufacturing technology, laser technology, astronavigation technology, ocean technology, military technology, new educational technology, etc., and at the same time Qigong technology with thinking control as its core will emerge. In the era of life, thinking and Qigong technology, technology proceeds very rapidly, global society and economy develop at a high speed, and globalization is accelerated. Life, thinking and Qigong technologies feature improvement of human internal energy and quality, purify human soul, reduce material desire, and greatly reduce environmental disruption. The overall （thinking and Qigong） technology takes the establishment of the cosmic era of human as its ultimate goal.展开更多
AIM:To investigate the effect of chloride intracellular channel 1(CLIC1) on the cell proliferation,apoptosis,migration and invasion of gastric cancer cells.METHODS:CLIC1 expression was evaluated in human gastric cance...AIM:To investigate the effect of chloride intracellular channel 1(CLIC1) on the cell proliferation,apoptosis,migration and invasion of gastric cancer cells.METHODS:CLIC1 expression was evaluated in human gastric cancer cell lines SGC-7901 and MGC-803 by real time polymerase chain reaction(RT-PCR).Four segments of small interference RNA(siRNA) targeting CLIC1 mRNA and a no-sense control segment were designed by bioinformatics technology.CLIC1 siRNA was selected using Lipofectamine 2000 and transfected transiently into human gastric cancer SGC-7901 and MGC-803 cells.The transfected efficiency was observed under fluorescence microscope.After transfection,mRNA expression of CLIC1 was detected with RT-PCR and Western blotting was used to detect the protein expression.Proliferation was examined by methyl thiazolyl tetrazolium and apoptosis was detected with flow cytometry.Polycarbonate membrane transwell chamber and Matrigel were used for the detection of the changes of invasion and migration of the two cell lines.RESULTS:In gastric cancer cell lines SGC-7901 and MGC-803,CLIC1 was obviously expressed and CLIC1 siRNA could effectively suppress the expression of CLIC1 protein and mRNA.Proliferation of cells transfected with CLIC1 siRNA3 was enhanced notably,and the highest proliferation rate was 23.3%(P = 0.002) in SGC-7901 and 35.55%(P = 0.001) in MGC-803 cells at 48 h.The G2/M phase proportion increased,while G0/G1 and S phase proportions decreased.The apoptotic rate of the CLIC1 siRNA3 group obviously decreased in both SGC-7901 cells(62.24%,P = 0.000) and MGC-803 cells(52.67%,P = 0.004).Down-regulation of CLIC1 led to the inhibition of invasion and migration by 54.31%(P = 0.000) and 33.62%(P = 0.001) in SGC-7901 and 40.74%(P = 0.000) and 29.26%(P = 0.002) in MGC-803.However,there was no significant difference between the mock group cells and the negative control group cells.展开更多
Suppliers become an essential factor of enterprise competitiveness. The analytic hierarchy process is a kind of Decision-making method with the combination of quantity and quality. This paper presents the evaluation s...Suppliers become an essential factor of enterprise competitiveness. The analytic hierarchy process is a kind of Decision-making method with the combination of quantity and quality. This paper presents the evaluation system of supplier selection, using Analytic Hierarchy Process (AHP) method to select the optimum supplier in Enterprise Resource Planning (ERP) enterprise. In order to reduce factor of manmade and increase exactitude, this paper innovates to use a real database in ERP system for evaluation. It also gives an example that one enterprise selects supplier.展开更多
Selecting optimization ship form scheme is an important content in the process of concept design of ship. Multi-objective fuzzy decision-making model for ship form demonstration is set up according to the fuzzy patter...Selecting optimization ship form scheme is an important content in the process of concept design of ship. Multi-objective fuzzy decision-making model for ship form demonstration is set up according to the fuzzy pattern-recognition theory. Weight coefficients of each target of ship form scheme are determined by information entropy and individual subjective partiality. This model is used to select the optimal ship form scheme, the example shows that the model is exact and the resuh is credible. It can provide a reference for choosing the optimization scheme of ship form.展开更多
文摘在初中英语教学中,笔者通过利用现代信息技术（Modern information technology）与＂导学式（Guided teaching method）＂相结合,对英语教学进行了改革尝试。实践证明,现代信息技术与＂导学式＂融合,像是给予了学生学习英语的＂点金术＂——使课堂教学改变了以往注入式教学的各种不良现象,使学生完全成为学习的主人,
基金Supported by the Key Science and Technology Program of Henan Province(162102110136)the Science and Technology Fund for Outstanding Young People of Henan Academy of Agricultural Sciences(2016YQ28)~~
文摘With the continuous progress on affinity peptide research, it has become more and more popular in pharmacology and medicine. lt is promising to study these viruses affinity peptide to treat infectious diseases. And the analysis on the virus affinity peptide with high selectivity and high sensitivity could provide valuable means for disease detection, treatment as wel as the study on the molecular mechanism of virus affinity peptide. Therefore, we reviewed the bioinformatics pre-diction technologies of computer simulation, molecular docking and homology model-ing, as wel as the research method on analyzing and screening virus affinity pep-tide, such as Phage display technology.
文摘Modern science and technology is being developed towards destroying human and human calls for a new era of science ＆ technology. Contemporary science is seeing a significant breakthrough and a new revolution of science and technology is beginning. The theoretical basis of the new revolution of science and technology is ＂state space-time＂, ＂the unity of physics and biology＂, ＂thinking motion and automatic organization of the universe＂ and ＂thinking＇s active control＂. The new revolution of science and technology takes material （microscopic particle） science, life （organic and inorganic life） science and thinking science as the fundamental sciences, takes life technology, new information technology, new material technology and new energy technology as the basis of the overall technological system, progresses in all aspects, including life technology, medicine, new material technology, new information technology, new energy technology, new robot technology, new manufacturing technology, laser technology, astronavigation technology, ocean technology, military technology, new educational technology, etc., and at the same time Qigong technology with thinking control as its core will emerge. In the era of life, thinking and Qigong technology, technology proceeds very rapidly, global society and economy develop at a high speed, and globalization is accelerated. Life, thinking and Qigong technologies feature improvement of human internal energy and quality, purify human soul, reduce material desire, and greatly reduce environmental disruption. The overall （thinking and Qigong） technology takes the establishment of the cosmic era of human as its ultimate goal.
基金Supported by The National Natural Science Foundation of China,No.30560151the Key Research Project of Guangxi Municipal Health Bureau,No.200824+1 种基金the Research Project of Guangxi Educational Department,No.201012MS062 and No. 2011105981002M204the Natural Science Foundation of Guangxi,No.0832113
文摘AIM:To investigate the effect of chloride intracellular channel 1(CLIC1) on the cell proliferation,apoptosis,migration and invasion of gastric cancer cells.METHODS:CLIC1 expression was evaluated in human gastric cancer cell lines SGC-7901 and MGC-803 by real time polymerase chain reaction(RT-PCR).Four segments of small interference RNA(siRNA) targeting CLIC1 mRNA and a no-sense control segment were designed by bioinformatics technology.CLIC1 siRNA was selected using Lipofectamine 2000 and transfected transiently into human gastric cancer SGC-7901 and MGC-803 cells.The transfected efficiency was observed under fluorescence microscope.After transfection,mRNA expression of CLIC1 was detected with RT-PCR and Western blotting was used to detect the protein expression.Proliferation was examined by methyl thiazolyl tetrazolium and apoptosis was detected with flow cytometry.Polycarbonate membrane transwell chamber and Matrigel were used for the detection of the changes of invasion and migration of the two cell lines.RESULTS:In gastric cancer cell lines SGC-7901 and MGC-803,CLIC1 was obviously expressed and CLIC1 siRNA could effectively suppress the expression of CLIC1 protein and mRNA.Proliferation of cells transfected with CLIC1 siRNA3 was enhanced notably,and the highest proliferation rate was 23.3%(P = 0.002) in SGC-7901 and 35.55%(P = 0.001) in MGC-803 cells at 48 h.The G2/M phase proportion increased,while G0/G1 and S phase proportions decreased.The apoptotic rate of the CLIC1 siRNA3 group obviously decreased in both SGC-7901 cells(62.24%,P = 0.000) and MGC-803 cells(52.67%,P = 0.004).Down-regulation of CLIC1 led to the inhibition of invasion and migration by 54.31%(P = 0.000) and 33.62%(P = 0.001) in SGC-7901 and 40.74%(P = 0.000) and 29.26%(P = 0.002) in MGC-803.However,there was no significant difference between the mock group cells and the negative control group cells.
文摘Suppliers become an essential factor of enterprise competitiveness. The analytic hierarchy process is a kind of Decision-making method with the combination of quantity and quality. This paper presents the evaluation system of supplier selection, using Analytic Hierarchy Process (AHP) method to select the optimum supplier in Enterprise Resource Planning (ERP) enterprise. In order to reduce factor of manmade and increase exactitude, this paper innovates to use a real database in ERP system for evaluation. It also gives an example that one enterprise selects supplier.
文摘Selecting optimization ship form scheme is an important content in the process of concept design of ship. Multi-objective fuzzy decision-making model for ship form demonstration is set up according to the fuzzy pattern-recognition theory. Weight coefficients of each target of ship form scheme are determined by information entropy and individual subjective partiality. This model is used to select the optimal ship form scheme, the example shows that the model is exact and the resuh is credible. It can provide a reference for choosing the optimization scheme of ship form.
